• Tuberculosis and Respiratory Diseases
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• 제59권2호
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• pp.157-163
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• 2005

목 적 : Myeloperoxidase (MPO)는 benzo(a)pyrene, aromatic amines과 같은 발암전구물질 활성화를 통해 폐암 발생에 관여한다. MPO 유전자 promoter 부위의 -463G>A 다형성은 MPO유전자의 발현량을 감소시킨다고 알려져 있다. 저자들은 MPO 유전자 promoter 부위의 -463G>A 다형성과 폐암 위험도의 상관 관계를 조사하였다. 방 법 : 경북대학교병원에서 폐암으로 진단된 432예를 대상으로 하였으며 대조군은 건강검진센타를 방문한 건강인 가운데 환자군과 연령 및 성을 match하여 무작위로 선택한 432명을 대상으로 하였다. 결 과 : MPO -463G>A의 유전자형은 폐암군의 경우 GG, GA, AA형이 각각 353명(81.7%), 76명(17.6%), 3명(0.7%)이였고 대조군의 경우 각각 356명(82.4%), 72명(16.7%), 4명(0.9%)으로 두 군간에 유의한 차이가 없었다. -463 AA+GA 유전자형은 -463 GG 유전자형에 비해 전체 폐암의 경우 위험도의 유의한 차이가 없었으며 (adjusted OR= 1.03, 95% CI= 0.72-1.47), 연령, 성별, 흡연력, 조직형으로 구분하였을 경우에도 유의한 차이가 없었다. 결 론 : MPO 유전자의 -463G>A 다형성은 한국인에서 폐암의 위험도를 결정하는 주요 인자가 아닌 것으로 생각된다.

• Journal of Veterinary Science
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• 제25권4호
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• pp.49.1-49.15
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• 2024

Importance: Endochondral ossification plays an important role in skeletal development. Recent studies have suggested a link between increased intracellular reactive oxygen species (ROS) and skeletal disorders. Moreover, previous studies have revealed that increasing the levels of myeloperoxidase (MPO) and osteopontin (OPN) while inhibiting NADPH oxidase 4 (NOX4) can enhance bone growth. This investigation provides further evidence by showing a direct link between NOX4 and MPO, OPN in bone function. Objective: This study investigates NOX4, an enzyme producing hydrogen peroxide, in endochondral ossification and bone remodeling. NOX4's role in osteoblast formation and osteogenic signaling pathways is explored. Methods: Using NOX4-deficient (NOX4^-/-) and ovariectomized (OVX) mice, we identify NOX4's potential mediators in bone maturation. Results: NOX4^-/- mice displayed significant differences in bone mass and structure. Compared to the normal Control and OVX groups. Hematoxylin and eosin staining showed NOX4^-/- mice had the highest trabecular bone volume, while OVX had the lowest. Proteomic analysis revealed significantly elevated MPO and OPN levels in bone marrow-derived cells in NOX4^-/- mice. Immunohistochemistry confirmed increased MPO, OPN, and collagen II (COLII) near the epiphyseal plate. Collagen and chondrogenesis analysis supported enhanced bone development in NOX4^-/- mice. Conclusions and Relevance: Our results emphasize NOX4's significance in bone morphology, mesenchymal stem cell proteomics, immunohistochemistry, collagen levels, and chondrogenesis. NOX4 deficiency enhances bone development and endochondral ossification, potentially through increased MPO, OPN, and COLII expression. These findings suggest therapeutic implications for skeletal disorders.

• 대한본초학회지
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• 제27권3호
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• pp.83-88
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• 2012

Objects : This study was performed in order to observe the effects of water extract of Dendrobii herba on intracerebral hemorrhage(ICH), Method : ICH was induced by the stereotaxic intrastriatal injection of bacterial collagenase type IV in Sprague-Dawley rats. After the water extracts of Dendrobii herba were administrated orally once a day for 3 days, hematoma volume, percentage of brain edema, expression of iNOS and MPO were observed using immunohistochemistry. Results : Rats fed with water extracts of Dendrobii herba showed reduction of hematoma volume and percentage of brain edema compared with controls. In addition, Infiltration of myeloperoxidase (MPO) expressing neutrophil and expression of inducible nitric oxide synthatase(iNOS) were significantly reduced in rats fed with water extracts of Dendrobii herba. Conclusion : These results demonstrated that water extracts of Dendrobii herba reduced brain damage of intracerebral hemorrhage(ICH) and subsequent ICH-induced cerebral edema, and inhibited neutrophil infiltration.

• 자원환경지질
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• 제19권1호
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• pp.57-66
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• 1986

The ore deposit of the Ohtani mine is one of representatives of plutonic tungsten-tin veins related genetically to acidic magmatism of Late Cretaceous in the Inner zone of Southwest Japan. Based on macrostructures of vein filling, three major mineralization stages are distinguished by major tectonic breaks. The constituents of ore minerals are scheelite, cassiterite, chalcopyrite, pyrrhotite, sphalerite, with small amounts of cubanite, stannite, galena, native bismuth, bismuthinite, arsenopyrite and pyrite. The relationship between the polymorphic variations of pyrrhotite and the kinds of the associated characteristic of ore mineral, in relation with hypogene mineralization, has been demonstrated. Pyrrhotite of stage I is predominantly of the hexagonal phase (Hpo>Mpo). Pyrrhotite of stage II is mainly of the monoclinic phase ($Hpo{\ll}Mpo$). Pyrrhotite of stage III is a single monoclinic phase ($Hpo{\ll}Mpo$). The compositions of the hexagonal pyrrhotite decrease in Fe content ranging from 47.44 atom % Fe in stage I to 46.88 atom % Fe in stage III.

• 대한약침학회지
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• 제20권1호
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• pp.52-56
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• 2017

Objectives: Neutrophils represent the front line of human defense against infections. Immediately after stimulation, neutrophilic enzymes are activated and produce toxic mediators such as pro-inflammatory cytokines, nitric oxide (NO) and myeloperoxidase (MPO). These mediators can be toxic not only to infectious agents but also to host tissues. Because flavonoids exhibit antioxidant and anti-inflammatory effects, they are subjects of interest for pharmacological modulation of inflammation. In the present study, the effects of rutin on stimulus-induced NO and tumor necrosis factor $(TNF)-{\alpha}$ productions and MPO activity in human neutrophils were investigated. Methods: Human peripheral blood neutrophils were isolated using Ficoll-Hypaque density gradient centrifugation coupled with dextran T500 sedimentation. The cell preparations containing > 98% granulocytes were determined by morphological examination through Giemsa staining. Neutrophils were cultured in complete Roswell Park Memorial Institute (RPMI) medium, pre-incubated with or without rutin ($25{\mu}M$) for 45 minutes, and stimulated with phorbol 12-myristate 13-acetate (PMA). Then, the $TNF-{\alpha}$, NO and MPO productions were analyzed using enzyme-linked immunosorbent assay (ELISA), Griess Reagent, and MPO assay kits, respectively. Also, the viability of human neutrophils was assessed using tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), and neutrophils were treated with various concentrations of rutin ($1-100{\mu}M$), after which MTT was appended and incubated at $37^{\circ}C$ for 4 hour. Results: Rutin at concentrations up to $100{\mu}M$ did not affect neutrophil viability during the 4-hour incubation period. Rutin significantly decreased the NO and $TNF-{\alpha}$ productions in human peripheral blood neutrophils compared to PMA-control cells (P < 0.001). Also, MPO activity was significantly reduced by rutin (P < 0.001). Conclusion: In this in vitro study, rutin had an anti-inflammatory effect due to its inhibiting NO and $TNF-{\alpha}$ productions, as well as MPO activity, in activated human neutrophils. Treatment with rutin may be considered as a therapeutic strategy for neutrophil-mediated inflammatory/autoimmune diseases.

• 대한예방의학회:학술대회논문집
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• 대한예방의학회 2004년도 제56차 추계 학술대회 연제집
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• pp.38.2-38.2
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• 2004

• 제어로봇시스템학회논문지
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• 제13권1호
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• pp.6-14
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• 2007

Modem version of supersonic jet fighter aircraft must have been guaranteed appropriate controllability and stability in HAoA(High Angle of Attack). Limit value of aircraft entering into the departure in HAoA is related to aircraft configuration design. But, the control law such as AoA and yaw-rate limiter is implemented in digital Fly-By-Wire flight control system of supersonic jet fighter to guarantee the aircraft's safety in HAoA. The HAoA flight control law have two parts, one is control law of departure prevention and the other is control law of departure recovery support. The control laws of departure prevention for advanced jet trainer consist AoA limiter, roll command limiter and rudder fader. The control laws of departure recovery support are consist yaw-rate limiter and MPO(Manual Pitch Override) mode. The guideline of pitch rocking using MPO mode is simple, but operating skill of pitch rocking is very difficult by the pilot with inexperience of departure situation. This paper addresses the design and validation of APR(Automatic Pitch Rocker) control law instead of MPO in order to automatic recovery without manual pitch rocking by the pilot. And, recovery characteristic with APR verifies by the nonlinear analysis and pilot evaluation.

• 한국항공우주학회지
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• 제33권7호
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• pp.85-91
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• 2005

군용항공기는 고받음각에서 적절한 조종성 및 항공기 이탈에 대한 안정성을 확보하고 있어야 한다. 고받음각에서 항공기가 이탈에 진입할 수 있는 한계값은 항공기 형상설계에 직결되는 문제이다. 하지만 현대의 고성능 전투기는 전기식 비행제어계통을 사용하여 고받음각 제어법칙을 설계함으로써 한계 값 내에서 항공기의 안정성을 보장하고 있으며 항공기가 이탈 시, 안전하게 회복할 수 있도록 고받음각 제어법칙을 설계한다. 현재 T-50에 적용되어 있는 고받음각 제어법칙은, 세로축 방향으로는 받음각 제한기 및 MPO(Manual Pitch Override) 모드, 가로-방향축으로는 고받음각 이탈제한기, 가로축 명령제한기, 방향축 조종사 명령제한기 및 스핀방지기가 설계되어 있다. 본 논문에서는 T-50 에 적용되어 있는 고받음각 제어법칙을 소개하며, 고받음각 비행시험을 통하여 항공기 안정성에 관한 연구를 수행하였다.

• 한방안이비인후피부과학회지
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• 제23권1호
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• pp.8-22
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• 2010

Objectives : The purpose of this study is to investigate the effects of Gyejijakyakjimo-Tang on the Allergic Contact Dermatitis caused by 2,4-dinitro-chlorobezene(DNCB). Methods : Twenty eight mice were divided into four groups ; normal, control, experimental group A and B. Control and experimental groups were induced allergic contact dermatitis by DNCB. Experimental group A was orally administered the Gyejijakyakjimo-Tang and experimental group B was orally administered the prednisolone. In this study, ear thickness measurement, auricle microphotograph observation, MPO(Myeloperoxidase) activity measurement, Reverse transcription-polymerase chain reaction(RT-PCR) analysis of the mRNA level of TNF-$\alpha$, IL-$1{\beta}$ were performed on these four groups. In addition, the effect of Gyejijakyakjimo-Tang on cell viability and the effect of Gyejijakyakjimo-Tang on the compound 48/80-induced histamine release from HMC and RPMC were measured. Results: 1. Both experimental group A and B had decreased ear thickness compared with control group In contact hypersensitivity assay. 2. In experimental group A, inflammatory edema was similarly observed comparing to control group. Nevertheless, inflammatory edema was obviously reduced in experimental group B. In both experimental group A and B, pathological lesion of dermatitis were alleviated. In addition, the numbers of infiltrated inflammatory cells were decreased compared with control group. 3. Compared to the normal group, there was a noticeable increase in MPO activity in control group. However, in experimental group A and B, it showed remarkable inhibition of the increase in MPO activity comparing with control group. 4. The level of expression of TNF-$\alpha$, IL-$1{\beta}$ in experimental group A and B were meaningfully lower than those in control group. 5. In MTT assay, the concentrations of Gyejijakyakjimo-Tang that were used on the test had no cytotoxicity. 6. Gyejijakyakjimo-Tang dose-dependently inhibited the compound 48/80-induced histamine release from both HMC and RPMC. Conclusions : According to above experiments, Gyejijakyakjimo-Tang was effective on allergic contact dermatitis.

• 대한본초학회지
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• 제29권1호
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• pp.19-26
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• 2014

Objectives : This study was performed in order to evaluate the effects of Salviae Miltiorrhizae Radix (SMR) water extract against the cerebral hemorrhage and the blood-brain barrier (BBB) impairment in the intracerebral hemorrhage (ICH). Method : ICH was induced by the stereotaxic intrastriatal injection of bacterial collagenase type IV in Sprague-Dawley rats. SMR was orally given three times every 20 hours during 3 days after the ICH induction. Hematoma volume, water content of brain tissue and volume of evans blue leakage were examined. Myeloperoxidase (MPO) positive neutrophils and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) were observed with immunofluorescence labeling and confocal microscope. Results : SMR significantly reduced the hematoma volume of the ICH-induced rat brain. SMR significantly reduced the water content of brain tissue of the ICH-induced rat brain. SMR reduced the percentage of the evans blue leakage around the hematoma on the caudate putamen compared to the ICH group, especially on the cerebral cortex. SMR significantly reduced the volume of the evans blue leakage level in the peri-hematoma regions of the ICH-induced rat brain. SMR significantly reduced MPO positive neutrophils in the peri-hematoma regions of the ICH-induced rat brain. SMR reduced the TNF-${\alpha}$ expression in peri-hematoma regions of the ICH-induced rat brain. TNF-${\alpha}$ immuno-labeled cells were coincided with MPO immuno-labeled neutrophils in peri-hematoma regions of the ICH-induced rat brain. Conclusion : These results suggest that SMR plays a protective role against the blood-brain barrier impairment in the ICH through suppression of inflammation in the rat brain tissues.