• The Korean Journal of Physiology and Pharmacology
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• 제5권3호
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• pp.279-285
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• 2001

Although only a minority of the infected individuals develops atrophic gastritis and the malignancy, factors governing clinical outcomes subsequent to Helicobacter pylori (H. pylori) infection have not yet been defined. H. pylori infection is characterized by extensive infiltration of neutrophils. Myeloperoxidase (MPO) in neutrophils amplifies the oxidative potential of hydrogen peroxides that induce gastric mucosal damage, thus MPO is suspected to play a role in H. pylori-induced gastric injury. Therefore, we explored the association of host MPO genetic polymorphism with atrophic gastritis upon H. pylori infection. Biopsy specimens taken from the gastric mucosa were examined histologically in 87 patients. The PCR-RFLP assay was used to characterize MPO genotypes. The distributions of MPO genotypes were MPO (G/G) 82% and MPO (G/A) 18%. None of MPO (A/A) genotype was observed. A strong positive correlation between the levels of neutrophil infiltration and gastric atrophy found only in MPO (G/G) but not in MPO (G/A) genotype. These results suggest that MPO genotype is a critical determinant in the pathogenesis of atrophic gastritis subsequent to H. pylori infection. Further works need to clarify the functional relevance of MPO genetic polymorphisms on gastric cell injury.

• Journal of Periodontal and Implant Science
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• 제25권3호
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• pp.733-740
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• 1995

This investigation was undertaken to determine the relationship between the amount of polymorphonuclear leukocyte(PMN) enzyme myeloperoxidase(MPO) in gingival crevicular fluid(GCF) collected from active or control site and gingival disease status described by clinical indices(gingival index, papillary bleeding index, pocket depth, periotron unit). The results were as follows : 1. MPO activity/site was greater at active sites than at control sites. 2. According to increasing the clinical parameters, MPO/sites was higher statistically (P<0. 01, P<0.05). 3. High MPO(unit/site) groups was higher statistically than low MPO(unit/site) groups in various clinical parameters. 4. Correlation coefficients between MPO(unit/site) and GI, MPO($unit/{\mu}l$ GCF) and periotron unit were 0.4782, -0.5901, respectively.

• 대한약리학회지
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• 제20권1호
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• pp.1-11
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• 1984

인체 백혈구에서 추출한 myeloperoxidase(MPO)에 의한 NADH산화 및 methional에서의 ethylene 생성을 관찰하여 cell-free MPO/$H_2O_2/Cl^-$ system에서 관여하는 oxygen metabolites가 무엇인지를 규명하기 위하여 본 실험을 수행하였다. 1) NADH는 MPO/$H_2O_2/Cl^-$에 의하여 산화됨을 확인하였다. 즉 MPO, $H_2O_2$ 및 $Cl^-$가 존재하는 medium에서 NADH의 산화는 azide와 catalase에 의하여, 그리고 medium에서 $Cl^-$를 제거하였을 때 완전히 제거되었다. 2) 이와같은 MPO/$H_2O_2/Cl^-$에 의한 NADH산화는 $^1O_2$ 제거물질인 1,4-diazabicyclo(2,2,2) octane(DABCO)에 의하여 완전히 억제 되었으나 $OH{\cdot}$의 제거물질인 mannitol, benzoate, formate 및 methanol에 의해서는 영향을 받지 아니하였다. 3) 또한 methinal을 MPO/$H_2O_2/Cl^-$으로 처리하였을 때는 ethylene이 전혀 검출되지 아니하였으나 기타 $OH{\cdot}$을 생성할 것으로 알려진 산화계인 xanthine/xanthine oxidase 및 $Ca^{++}-H_2O_2$에 의해서는 현저한 ethylene생성을 관찰하였다. 이상의 결과는 Cell-free MPO/$H_2O_2/Cl^-$ 산화계에서는 $^1O_2$이 산화반응에 관여하는 주된 산소 대사물이며 $OH{\cdot}$은 생성되지 아니함을 알 수 있었다.

• 대한의생명과학회지
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• 제6권4호
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• pp.281-288
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• 2000

심장수술에 따른 백혈구의 활성화는 여러 염증성 물질을 생성시켜 수술 후 병태생리에 깊이 관여한다. 본 연구는 심장수술 동안 백혈구의 과립성분인 myeloperoxidase의 농도 변화와 심장 및 폐에 대한 영향을 규명하기 위해 시행되었다. 심장수술 전후로 말초혈액 총 백혈구 수 및 분획세포 수와 관상정맥 혈액의 myeloperoxidase 농도 (MPO) 및 troponin-T 농도 (TnT), 그리고 폐혈관 저항을 측정하였다. 수술 직후, 호중구 수의 상승에 기인한 총 백혈구 수의 증가가 있었고 (p＜0.01), MPO 및 TnT 역시 증가하였으나 (p＜0.05), 폐혈관 저항은 감소하였다(p＜0.05). 수술 후, MPO는 TnT, 총 백혈구 수, 호중구 수, 그리고 심장수술 소요시간과 각각 양의 상관관계가 있었다(p＜0.05). 수술 후 폐혈관 저항은 총 백혈구 수 및 호중구 수와 각각 음의 상관성이 있었다(p<0.05). 본 연구의 결과들은 심장수술이 호중구로 부터 MPO 분비를 증가시키며, 유리된 MPO는 폐보다는 심장에 유해한 효과를 미칠 수 있고 호중구의 또 다른 기전이 수술 후 폐 손상에 관여할 수 있음을 시사하고 있다.

• Bulletin of the Korean Chemical Society
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• 제31권11호
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• pp.3259-3264
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• 2010

A label-free amperometric immunosensor has been proposed for the detection of myeloperoxidase (MPO) in human serum. To fabricate such an immunosensor, a composite film consisting of N,N-dimethylformamide (DMF), multiwall carbon nanotubes (MWCNTs) and 1-ethyl-3-methyl imidazolium tetrafluoroborate ($EMIMBF_4$) suspension was initially formed on a glassy carbon electrode (GCE). Then cerium dioxide ($CeO_2$) dispersed by chitosan was coated on the GCE. After that, MPO antibodies (anti-MPO) were attached onto the nano$CeO_2$ surface. With a noncompetitive immunoassay format, the antibody-antigen complex formed between the immobilized anti-MPO and MPO in sample solution. The immunosensor was characterized by cyclic voltammetry, transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The factors influencing the performance of the immunosensor were studied in detail. Under optimal conditions, the current change before and after the immunoreaction was proportional to MPO concentration in the range of 5 to $300\;ng\;mL^{-1}$ with a detection limit of $0.2\;ng\;mL^{-1}$.

• 한국전문물리치료학회지
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• 제10권2호
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• pp.61-70
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• 2003

The purposes of this study was to evaluate the effect of low power GaAsAl laser on tissue contraction in a linear incision wound on rat skin. The linear incision wound was made on the midline of the backside in the experimental animals. Low power laser applications with different intensities such as 3, 6, or 10 mW were applied to the experimental animals twice a day for 10 days. On either the seventh or tenth postoperative day, the quantitative analysis of the inflammatory reaction surrounding the linear incision wounds on the rats were performed using enzymatical analysis of myeloperoxidase (MPO) activity. The number of neutrophil was $.07-1.0{\times}106/m{\ell}$ from a normal blood sample that was obtained from the normal experimental animals. Each concentration of neutrophil showed .04-.62 unit activity of MPO. Therefore, the 6 unit activity of MPO per neutrophil was $.57{\pm}.014{\times}10^{-6}$ unit. On the 7th and 10th post operative day, non treated tissues demonstrated increased MPO activity as compared to that of normal tissue. These data indicated that the inflammatory reaction of tissue was induced after wound induction and the MPO activity were increased in the inflammed tissues. While both 3 mW or 6 mW intensity of laser treatments did not affect the tissue MPO activity, 10 mW intensity of laser treatment significantly decreased the tissue MPO activity on the 7th and 10th post operative day. These data demonstrated that only 10 mW intensity of laser treatment successfully suppressed tissue inflammatory reaction after wound induction. In conclusion, these findings suggested that 10 mW of GaAIAs laser treatments effectively suppressed the inflammatory reaction of tissue that was induced during the wound healing process.

• Toxicological Research
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• 제20권3호
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• pp.187-193
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• 2004

This study aimed to determine the association of maternal oxidative stress and adverse pregnancy outcome with serum vitamin C concentration and a myeloperoxidase (MPO) genetic polymorphism during pregnancy. We investigated 450 pregnant women who visited Ewha Womans University Hospital for prenatal care during gestational weeks 24～28. During the second trimester, we measured serum vitamin C levels and urinary 8-hydroxyde-2'-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) as an oxidative stress biomarker. We determined the presence of a maternal MPO polymorphism (G-to-A substitution at nucleotide 463) using a PCR-RFLP assay. We compared the level of oxidative stress and birth weight with the vitamin C concentration and the presence of the MPO polymorphism. The mean level of maternal oxidative stress tended to be higher and the birth weight lower for MPO type A/A than for types A/G and G/G. Vitamin C levels above the 75 percentiles were associated with reduced concentrations of urinary MDA and 8-OHdG but increased birth weight. Our data demonstrate that oxidative stress and neonatal birth weight are associated with the MPO genetic polymorphism, with the association modified by the maternal vita-min C levels.

• 대한한방내과학회지
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• 제26권2호
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• pp.379-389
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• 2005

Objectives: It is well known that aging and aging-related diseases are linked to the increased level of oxidative stress caused by reactive oxygen species(ROS) and reactive nitrogen species(RNS). Nonprotein-SH decreases during aging, while substances such as ROS, nitric oxide(NO), peroxynitrite($ONOO^-$), myeloperoxidase(MPO), and dityrosine show a significant increase. This study investigated the effect of Ichungwhan on the aging process by examining its effect on the generation of the above-mentioned substances. Methods: Four comparison groups of SD rats were used. They were 6 month-old rats, 24 month-old rats, and 24 month-old rats fed with food containing 0.1% and 0.3% of Ichungwhan extract. The amount of NO, $ONOO^-$, and ROS in the rats' kidneys were examined using a fluorescence microplate reader. The reagents used for this purpose include: dihydrorhodamine 123 (DHR 123), 2',7' -dichlorodihydrofluorescein, diacetate(DCFDA), and 4,5-diaminofluorescein(DAF-2). A spectrophotometer was used to investigate the reactivity of nonprotein-SH and myeioperoxidase(MPO), using reagents such as trichloroacetic acid(TCA) and tetramethylbenzidine(TMB). The amounts of MPO protein and dityrosine were measued by western blot. Results: The observed effects of Ichungwhan on rats were as follows: increase of nonprotein-SH; effective decrease of RNS level by suppression of the generation system of $ONOO^-$ and NO; decrease of ROS level; decrease of the MPO reactivity and the subsequent reduction of amount of MPO protein; retardation of dityrosine formation. It can be hypothesized, therefore, that Ichungwhan affects both the earlier and later phases of the molecular inflammatory process, and retards the aging process. Conclusions: Empirical evidence in this study supports a role for Ichungwhan in generation mechanisms of aging process-linked substances ROS, NO, $ONOO^-$, nonprotein-SH, MPO and dityrosine. Affects contrary to the aging process observed in rats beg further empiricism to investigate potential application of Ichungwhan as a medication for age-related diseases in humans.

• 교통기술과정책
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• 제2권2호
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• pp.53-78
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• 2005

지역교통계획과정에서 중요한 요소의 하나는 교통계획과 실행을 위한 관할구역 판단에서의 일관성인데 각 관할기관이 그 관할구역의 범위 안에서만 교통시설에 대한 권한을 갖는다. 계획과정에서 그러한 일관성을 획득하기 위해서는 MPO들이 협력, 계속성, 종합의 3C(cooperation, continuing, cooperative)와 종합적이고 일관성 있는 교통계획과정(요구되긴 하나 연방정책이나 프로그램에 의해 꼭 제약받지는 않는)을 갖는 것이 필수 불가결하다. MPO들은 도시화 지역을 위한 교통계획과 프로그램을 개발하는데 있어 주와 지역도시들, 관계기관들과 협력적이어야 한다. 이 교통계획과정은 지역도시와 군들의 종합적으로 계획된 개발과 일관성 있는 계획과 프로그램들로 결과되어 진다. 이 협력적인 교통의사결정과정은 멤버인 관할기관이 그 자격을 위한 지역교통이슈와 계획교통개선을 하기 위한 포럼을 제공한다. 각 도시와 군의 레벨에서, 계획 부서의 교통계획가들이 지역자본시설계획(Capital Facility Plan, CFP)과 지역 교통개선프로그램(Transportation Improvement Program, TIP)을 통해 계획과정을 수행하는데 반해, 공공사업(Public Works)부서의 교통기술자들은 예측되는 혹은 예상되는 교통수요를 맞추는 시설을 설계하고 건설하는 일을 하도록 되어 있다. MPO 스태프들은 지역도시와 군들에 지역맥락과 연방정책 및 프로그램들의 가이드 내에서 종합적이고 일관성 있는 교통자료와 정보를 제공하도록 되어 있다. MPO들은 특히 지역교통수요분석과 예측에서 그러한 전문성이 없는 도시와 군들에 기술적 조력을 제공하도록 되어 있다. MPO의 지역교통계획에 대한 원칙과 가이드는 도시들, 군들, 다른 기관들의 참여(상향식 계획과정인 공공참여를 포함하여)에 의해 개발되어진다. 모든 참여자들은 지역교통 계획에서 지역 목적과 목표를 달성하기 위해 함께 일하게 된다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권8호
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• pp.4739-4742
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• 2013

Carboplatin, a second generation platinum drug, is widely used to treat different types of cancers. However, myelosuppression remains a major consideration in its use. Genetic polymorphisms of enzymes involved in drug disposition can influence therapeutic outcome. The homozygous null deletion of phase II metabolic gene GSTT1 that abolishes its xenobiotic- detoxifying ability may be associated with carboplatin toxicity. Further, since carboplatin generates oxidative stress, polymorphisms of oxidative stress genes that regulate the cellular level of free radicals may have important roles in generating drug- related adverse effects. We here investigated the null polymorphism of GSTT1, and the -463G>A promoter polymorphism of oxidative stress gene myeloperoxidase (MPO) for carboplatin toxicity in a population of northern India. Cancer patients who were treated with carboplatin, and developed toxicity was considered. The study group comprised of 10 patients who developed therapy- related adverse effects. Peripheral blood was taken from patients for DNA isolation. GSTT1 null genotype was determined by conducting duplex PCR and MPO-463 G>A was determined by PCR followed by RFLP. Hematologic toxicity was experienced by 5 patients, 2 of them had grade 3 and 4 toxicity and 3 others had grade 2 toxicity. They also had gastrointestinal (GI) toxicity. Remaining 5 individuals developed GI toxicity but no hematological toxicity. While GG homozygous of MPO was present in majority of patients having hematologic toxicity (in 4 out of 5 individuals), one A allele (AG genotype) was present in 4 patients who did not have any hematological toxicity. Thus variant A allele of MPO -463G>A may be related to lower hematological toxicity. These preliminary data, however, are required to be confirmed in larger studies along with other relevant polymorphisms.