• Title/Summary/Keyword: MPB70

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Detection of Mycobacterium bovis in the lymph node of tuberculin positive cattle by guanidium isothiocyanate/silica DNA extraction and polymerase chain reaction

  • Cho, Yun-Sang;Jung, Suk-Chan;Yoo, Han-Sang;Kim, Jong-Man
    • Korean Journal of Veterinary Service
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    • v.30 no.2
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    • pp.233-241
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    • 2007
  • Tuberculin positive cattle without gross tubercle lesions should be confirmed by the bacteriological examination to determine the state of the infection. To overcome the time-consuming and laborious identification by culture and biochemical tests, polymerase chain reaction (PCR) has been used to identify Mycobacterium bovis. Due to various lipids in the cell wall of Mycobacterium spp, novel methods of DNA extraction from Mycobacterium spp have been developed. In this study, a newly developed guanidium isothiocyanate/silica DNA extraction method was directly applied to specimens from the tuberculin positive cattle. DNAs were directly extracted from the lymph nodes and the major polymorphic tandem repeat (MPTR) and mycobacterial protein of BCG 70 (MPB70) were amplified using PCR. The DNA extraction method using guanidium isothiocyanate/silica was efficient and safe, and the MPTR and MPB70 primers were specific to M bovis. Therefore, MPTR and MPB70 PCRs will be useful for the detection of M bovis in the lymph node from skin-test positive cattle.

Effect of Sulfate and Heavy Metals on Methanogenic Activation of in the Anaerobic Digestion of Tannery Wastes (피혁폐수의 혐기성 소화시 황산염과 중금속이 메탄균 활성에 미치는 영향)

  • Shin, Hang Sik;Oh, Sae Eun
    • Journal of the Korea Organic Resources Recycling Association
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    • v.4 no.1
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    • pp.13-21
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    • 1996
  • For treating tannery wastewater containing high sulfate and heavy metals, test was performed to assess their performance, competition between SRB (sulfate reducing bacteria) and MPB (methane producing bacteria), and the activity of MPB according to change of chromium concentrations. COD removal efficiency was above 70% at VLR (volumetric loading rate) of 2.0 gCOD/I.day and HRT (hydraulic retention time) of 18hrs at $35^{\circ}C$. In the competition between SRB and MPB, about 15% of the removed COD was utilized by SRB in the begining, but it became 43% at the end. It indicated that MPB was strongly suppressed by the occurrence of significant sulfate reduction since a large electron flow was uptaken by SRB. For the entire experiment, removal efficiencies of chromium concentration were more than 90%. Despite high removal efficiencies of chromium concentration, performance of reactor did not change significantly during the experimental periods. Expecially, chromium (III) is tannery wastewater is less toxic than chromium (VI).

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Electro-optic Characteristics of 7.5/70/30 PLZT Ceramics According to the Second Stage Sintering Time (2단 소성시간이 7.6/70/30 PLZT 세라믹의 전기적 특성에 미치는 영향)

  • Park, Chang-Yub;Chong, Ik-Chae;Jeong, Hoy-Seung
    • Proceedings of the KIEE Conference
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    • 1987.11a
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    • pp.220-223
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    • 1987
  • In this paper, electro-optic characteristics of PLZT 7.6/70/30 composition in MPB regions, which antiferroelectric and ferroelectric phases coexist together, were studied. As sintering times are increased, Curie temperatures ore linearly decreased. And the antiferroelectric phase becomes more stronger when Curie temperatures are decreased. The crystal structure investigated by lie of X-ray analysis is proved to cubic in the thermally depoled specimen and rhombohedral in the poled specimen. Accordingly, the applicability of 7.6/70/30 specimen as image storage and display device is expected by the method for applying field induced reversible transitions between antiferroelectric(AFE) and ferroelectric(FE) phase.

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Activity of Methanogens in the High Rate Anaerobic Digestion of Swine Wastewater Containing High Ammonia (고농도 암모니아를 함유한 돈사폐수의 고율혐기성 소화시 메탄균의 활성연구)

  • Oh, Sae-Eun;Lee, Chae-Young
    • Journal of Korean Society of Environmental Engineers
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    • v.22 no.5
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    • pp.981-987
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    • 2000
  • Upflow anaerobic sludge blanket(UASB) reactor was operated for treating swine wastewater containing high ammonia nitrogen to assess their performance and toxicity of free ammonia concentration. In the reactor, chemical oxygen demand(COD) removed about 70% at $2.6kgCOD/m^3.day$ of organic loading rate(OLR) and 3 days of hydraulic retention time (HRT), while it was decreased when OLR and HRT was maintained $7kg\;COD/m^3.day$ and 2 days, respectively. Also UASB reactor was evaluated the activity of methane producing bacteria(MPB) according to change of free ammonia concentrations, MPB activity of applied sludge in the 500 and $1000mg-N/{\ell}$ of free ammonia concentration was inhibited by 4% and 40%, respectively. This clearly showed that free ammonia concentration less than $500mg-N/{\ell}$ showed no inhibition to MPB in anaerobic treatment of organics, UASB reactor was stabilized easily less than $1000mgVSS/{\ell}$ due to degradation of organic solids by the high activities of anaerobes.

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Change of Morphologic Pelvis and Deviation According to Gender, Age -Radiological Pelvimetry- (성별과 나이에 따른 골반 형태 변화와 골반 변위 -방사선영상을 이용한 골반 계측-)

  • Kang, Yeong-Han;Kim, Seon-Chil
    • Journal of radiological science and technology
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    • v.34 no.2
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    • pp.91-96
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    • 2011
  • The purpose of this study was to analyze the change of morphologic pelvis according to age and gender. Pelvis AP(anterior-posterior) projection were taken from 382 individuals (men: 194, women: 188) at the age of twenty and older. We measured four values of pelvis using CR (computed radiological system) including maximum pelvic breadth (MPB), sacral breadth (SB), transverse diameter of pelvic inlet (TD), and distance between femoral heads (DFH). Average (t-test, ANOVA) and Pearson correlation were calculated for comparison. While there was no significant difference of gender in case of MPB, significant differences were observed in SB (men 97.76 mm, women 102.00 mm), TD (men 138.0 mm, women 149.79 mm), and DFH (men 141.94 mm, women 150.39 mm). The DFH values were negatively correlated with the age (men r=-0.280, women r=-0.207). The morphological changes of pelvis were measured high in the men of 50's and in the women of 60's and 70's. The SB, TD and DFH were greater in the women than in the man. As the ages get higher, the deviation of pelvis were gradually increased, which indicates that the morphological changes of pelvis with respect to the age reflects an aging process.

Effect of MnO2 Addition on Dielectric and Piezoelectric Properties of 0.985[Li0.04(Na0.545K0.46)0.96(Nb0.81Ta0.15Sb0.04)]O3 Ceramics (MnO2 첨가가 0.985[Li0.04(Na0.545K0.46)0.96(Nb0.81Ta0.15Sb0.04)]O3+0.015KNbO3 세라믹스의 유전 및 압전 특성에 미치는 영향)

  • Kim, YouSeok;Yoo, JuHyun
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.27 no.6
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    • pp.361-366
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    • 2014
  • $MnO_2$-doped $0.985[Li_{0.04}(Na_{0.545}K_{0.46})_{0.96}(Nb_{0.81}Ta_{0.15}Sb_{0.04})]O_3+0.015KNbO_3(0.985LNKNTS+0.015KNbO_3)$ lead-free ceramics were fabricated by conventional solid state method to develop excellent dielectric and piezoelectric properties. The result of X-ray diffraction patterns obviously indicated that all of the specimen has pure perovskite structure without secondary phase. In addition, orthorhombic phase and coexistance region of orthorhombic-tetragonal phase (MPB) were observed with amount of $MnO_2$. The optimal values of ${\rho}$=4.70 $g/cm^3$, $d_{33}=238$ pC/N, $k_P=0.46$, $Q_m=121$, ${\varepsilon}_r=849$, and $T_C=225^{\circ}C$ were obtained at 0.01 mol% $MnO_2$ doped $0.985LNKNTS+0.015KNbO_3$ ceramics sintered at $990^{\circ}C$ for 5 h, respectively. Hence, it was indicated that the suitable amount of $MnO_2$ could improve the electrical properties of $0.985[Li_{0.04}(Na_{0.545}K_{0.46})_{0.96}(Nb_{0.81}Ta_{0.15}Sb_{0.04})]O_3+0.015KNbO_3$ ceramics.

Processing and Properties of RAINBOW Piezoelectric Actuator (RAINBOW 압전 액츄에이터의 제조와 물성)

  • Paik Jong-Hoo;Lim Eun-Kyeong;Kim Chang-il;Lee Mi-Jae;Jee Mi-Jung;Choi Byung-Hyun;Kim Sei-Ki
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.19 no.3
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    • pp.222-227
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    • 2006
  • RAINBOW(Reduced And Internally Biased Oxide Wafers) are a new class of high-displacement, piezoelectric actuator produced by selectively removing oxygen from one surface of ceramic using a high-temperature chemical reduction process. In this paper, RAINBOW actuator materials of $0.4Pb(Ni_{1/3}Nb_{2/3})O_3-0.6Pb(Zr_{x}Ti_{1-x})O_3$ ceramics were prepared. Its dielectric and piezoelectric properties were investigated in the vicinity of MPB. The piezoelectric properties showed the maximum value of ${\epsilon}r$ = 4871, $d_{33}$ = 610 ($10^{-12}$ m/V), $d_{31}$ = -299 ($10^{-12}$ m/V), $k_{33}$ = $71\%$, Qm = 70, in $0.4Pb(Ni_{1/3}Nb_{2/3})O_{3}-0.6Pb(Zr_{405}Ti_{595})O_3$ composition sintered at $1250^{\circ}C$. The strain - electric field characteristics of RAINBOW actuator were significantly improved comparison with the conventional bulk actuator. The prepared RAINBOW actuator showed about $390\;{\mu}m/100\;V$ displacement.

Effects of $Ba(La_{1/2}Nb_{1/2})O_3$ content and Zr/Ti composition ratio on electrooptic properties of the $Ba(La_{1/2}Nb_{1/2})O_3-Pb(Zr, Ti)O_3$ ceramics ($Ba(La_{1/2}Nb_{1/2})O_3$의 고용량과 Zr/Ti 조성비가 $Ba(La_{1/2}Nb_{1/2})O_3-Pb(Zr, Ti)O_3$ 세라믹의 전기광학 특성에 미치는 영향)

  • 류기원;이성갑;배선기;이영희
    • The Transactions of the Korean Institute of Electrical Engineers
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    • v.44 no.5
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    • pp.644-648
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    • 1995
  • Transparent xBa(La$_{1}$2/Nb$_{1}$2/)O$_{3}$-(1-x)Pb(Zr, Ti)O$_{3}$ (x=8.5, 9.0[mol.%], Zr/Ti=70/30~40/60) ceramics were fabricated by the two-stage sintering method, the electrooptic properties were investigated with Ba(La$_{1}$2/Nb$_{1}$2/)O$_{3}$ content and Zr/Ti composition ratio. Decreasing the Zr/Ti ratio, the electrooptic property was changed from a quadratic electrooptic effect to a linear electrooptic effcet. In the BLN-PZT 9.0/50/50 specimen having the tetragonal structure, the linear electrooptic coefficient had the highest value of 6.01*10$^{-10}$ [m/V] and in the BLN-PZT 9.0/55/45 specimen which located near the MPB region, the quadratic electrooptic coefficient had the highest value of 10.53*10$^{-16}$ [m$^{2}$/V$^{2}$].

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Structural and Piezoelectric Properties of MnO2-Doped PZT-PSN Ceramics for Ultrasonic Vibrator (초음파 진동자용 MnO2가 Doping된 PZT-PSN 세라믹스의 구조 및 압전 특성)

  • Cha, Yoo-Jeong;Kim, Chang-Il;Kim, Kyoung-Jun;Jeong, Young-Hun;Lee, Young-Jin;Lee, Hai-Gun;Paik, Jong-Hoo
    • Korean Journal of Materials Research
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    • v.19 no.4
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    • pp.198-202
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    • 2009
  • For use in ultrasonic actuators, we investigated the structural and piezoelectric properties of $(1\;-\;x)Pb(Zr_{0.515}Ti_{0.485})O_3$ - $xPb(Sb_{1/2}Nb_{1/2})O_3$ + 0.5 wt% $MnO_2$ [(1 - x)PZT - xPSN + $MnO_2$] ceramics with a variation of x (x = 0.02, 0.04, 0.06, 0.08). All the ceramics, which were sintered at $1250^{\circ}C$ for 2 h, showed a typical perovskite structure, implying that they were well synthesized. A homogeneous micro structure was also developed for the specimens, and their average grain size was slightly decreased to $1.3{\mu}m$ by increasing x to 0.8. Moreover, a second phase with a pyrochlore structure appeared when x was above 0.06, which resulted in the deterioration of their piezoelectric properties. However, the 0.96PZT-0.04PSN+$MnO_2$ ceramics, which corresponds with a morphotropic phase boundary (MPB) composition in the (1 - x)PZT - xPSN + $MnO_2$ system, exhibited good piezoelectric properties: a piezoelectric constant ($d_{33}$) of 325 pC/N, an electromechanical coupling factor ($k_p$) of 70.8%, and a mechanical quality factor ($Q_m$) of 1779. The specimens with a relatively high curie temperature ($T_c$) of $305^{\circ}C$ also showed a significantly high dielectric constant (${\varepsilon}_r$) value of 1109. Therefore, the 0.96PZT - 0.04PSN + $MnO_2$ ceramics are suitable for use in ultrasonic vibrators.

Effect of $Cr_2O_3$ and $Nb_2O_5$ Additives on the Microstructure and Piezoelectric Properties of PZT Ceramics for Piezoelectric Composite Sensor (압전복합센서용 PZT 세라믹스의 미세구조 및 압전특성에 미치는 $Cr_2O_3$$Nb_2O_5$ 첨가효과)

  • Paik, Jong-Hoo;Eom, Heyung-Keun;Lim, Eun-Kyeong;Kim, Chang-Il;Lee, Mi-Jae;Choi, Byung-Hyun;Kim, Sei-Ki
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2005.11a
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    • pp.27-28
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    • 2005
  • 본 연구에서는$Cr_2O_3$$Nb_2O_5$ 를 첨가한 $Pb(Zr_{0.54}Ti_{0.48})O_3$ 세라믹스에서 $Nb_2O_5$ 첨가량에 따른 소결 및 압전, 유전특성을 조사하였다. $Pb(Zr_{0.54}Ti_{0.48})O_3$ + 0.2 wt% $Cr_2O_3$ + wt% $Nb_2O_5$ ($0.{\sim}2wt.%$)의 첨가량에 따른 압전, 유전특성 및 미세구조에 관해 연구하였다. 본조성에서 $Nb_2O_5$ 첨가량이 증가함에 따라 입경의 크기는 증가하였으며, 0.5 wt% $Nb_2O_5$ 첨가조성에서 $4\sim5\mu m$의 최대 평균입경을 보이다가 그 이상의 첨가 조성에서 급격히 감소하였다. 유전상수와 kp 는 $Nb_2O_5$ 첨가량이 1.0 wt% 조성까지 증가하였다가 그 이상 조성에서 감소하였다. $Nb_2O_5$ 첨가량이 증가함에 따라 삼방정(rhombohedral)구조에서 정방정(tetragonal)구조로 상전이 일어났으며, 본 조성의 상경계 영역인 0.5 wt% 조성에서 $\varepsilon_r$ = 730, $k_p$ = 0.72, $d_{33}$ = 450, $g_{33}$ = 70의 우수한 압전 특성을 나타내었다. 이러한 조성은 접촉센서용 복합압전소재의 실용가능성을 제시하였다.

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