• Title/Summary/Keyword: MP90

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Measurement of Dynamic Fracture Toughness Using Chevron Notched Ceramic Specimen (세브론노치 세라믹시편을 이용한 동적파괴인성측정)

  • Lee, Yeon-Soo;Lee, Young-Sun;Park, Rae-Seok;Moon, Young-Deuk;Yoon, Hi-Seak
    • Journal of the Korean Society for Precision Engineering
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    • v.18 no.5
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    • pp.98-104
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    • 2001
  • A dynamic fracture toughness test method with a chevron notched ceramic specimens is proposed. The notch angles of the chevron specimens were 90, 100$^{\circ}$and 110$^{\circ}$. Finite element analysis(FEA) were done to determine the geometrical properties of chevron-notch specimens according to notch angles. The static fracture toughness of the chevron notched alumina specimen was 3.8MP$\alpha$√m similar to that of the general fracture specimen with a precrack. Dynamic fracture toughness was 4.5 MP$\alpha$√m slightly higher than the static one. These research showed the possibility of the split Hopkinson pressure bar test method using the newly proposed chevron notched specimens to get the dynamic fracture toughness of extremely brittle materials such as ceramics.

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Expression of Mouse Adenosine Deaminase Gene in Transgenic Tobacco (Nicotiana tabacum L.) (형질전환 연초(Nicotiana tabacum L.)의 Mouse Adenosine Deaminase 유전자 발현)

  • 양덕춘;박지창;최광태;이정명
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.4
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    • pp.195-200
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    • 1995
  • The mammalian adenosine deaminase(ADA) gene was stably expressed in transgenic tobacco plane. The chimeric ADA gene 35S/35S/AMV/ADA/Tnos, has been constructed. This chimeric gene was introduced into the binary vector pRD400, which was thereafter mobilized into Agrobacterium tumefaiens strain MP90 harboring disarmed Ti-plasmid. The resulting strains were used to transform Nicofiana tabacum L. using the leaf disc. Incorporation of the chimeric gene into plant were confirmed by PCR and Northern blot analyses. Immunoblot analysis showed that ADA protein was successfully synthesized in the transgenic tobacco plants.

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Automatic defect detection using intensity and shape information in industrial CT (산업용 CT 영상에서 밝기값 및 형태 정보를 이용한 기공 결함 자동 검출)

  • Ji, Hye-Rim;Hong, Helen
    • Proceedings of the Korean Information Science Society Conference
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    • 2012.06c
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    • pp.415-417
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    • 2012
  • 본 논문에서는 산업용 CT 영상에서 다중 해상도 기반의 밝기값 정보와 형태 정보를 이용하여 내부 기공결함을 정확하고 빠르게 검출하는 기법을 제안한다. 첫째, 대용량 CT 데이터에서 계산량을 줄이기 위하여 1/2 해상도로 변환 후 관심영역을 자동 산정하고, 링 또는 금속 인공물 등의 잡음을 제거하기 위해 비등방성 확산 필터링을 수행한다. 둘째, 기공 결함 후보군을 검출하기 위해 밝기값 기반의 결함 검출 기법을 제안한다. 셋째, 결함 검출의 민감도를 향상시키기 위해 형태 정보를 이용한 기공 결함 검출 기법을 제안한다. 넷째, 수행시간 가속화를 위하여 다중 해상도 영상 처리 및 Open MP를 적용한다. 제안방법의 평가를 위하여 육안평가와 정확성 평가, 수행시간을 측정하였다. 정확성 평가는 실제 기공 결함과 제안방법 적용 후 결함 간 중복 픽셀 수로 측정하였다. 실험 결과 평균 중복 픽셀 비율은 91%로 측정되었고, 가장 큰 비율은 99%, 가장 작은 비율은 80%로 측정되었다. 다중 해상도 기법 및 Open MP를 적용함으로써 해상도 데이터 수행시간보다 90% 가속화되었다.

Expression of Chitinase Gene in Solanum tuberosum L.

  • Park, Kyung-Hwa;Yang, Deok-Chun;Jeon, Jae-Heung;Kim, Hyun-Soon;Joung, Young-Hee;Hyouk Joung
    • Journal of Plant Biotechnology
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    • v.1 no.2
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    • pp.85-90
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    • 1999
  • In order to protect fungal diseases, leaf disc explants of Solanum tuberosum cultivar, Belchip, was infected with an Agrobacterium MP90 strain containing chimeric gene construct, consisting of antibiotic resistance and chitinase gene driven by the CaMV 35S promoter, for transformation. Regenerated multiple shoots were selected on a medium containing kanamycin and carbenicillin after exposure to Agrobacterium. The presence and integration of the npt II and chitinase gene were confirmed by polymerase chain reaction(PCR). Northern blot analysis indicated that the genes coding for the enzyme could be expressed in potato plants. The chitinase activity of transgenic potato plants was higher than the control potato.

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Transformation of Potato using the Phosphinothricin Acetyltransferase Gene as the Selectable Marker Gene (감자의 형질전환을 위한 표지유전자로서 Phosphinothricin Acetyltransferase 유전자의 이용)

  • Jeong, J.H.;Yang, D.C.;Bang, K.S.;Han, S.S.
    • Korean Journal of Weed Science
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    • v.18 no.3
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    • pp.205-213
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    • 1998
  • This experiment was carried out to produce herbicide resistant potatoes hawing only chimeric phosphinothricin acetyltransferase (PAT) genes without using antibiotic selectable marker. The pDY502 vector having only PAT gene was reconstructed for transformation of potato. The reconstructed vector was introduced to Agrobacterium tumefaciens MP90 disarmed, and they were used for potato transformation. Hormonal requirement for plant regeneration from leaves and stem explants of potato was investigated. From this experiment, MS medium treated with IBA 0.1 mg/L + BA 0.5 mg/L was the best for potato regeneration, and the ratio of shoot regeneration was 54% for leaf and 46% for stem in that condition. For transformation, explants of potato leaves and stems were cocultured with A. tumefaciens MP90 containing reconstructed vector harvoring only PAT gene. When the potato explants were placed on various concentrations of bialaphos and all the potato explants were dead on medium with over 5.0mg/L bialaphos. By this selection methods, the explants cocultured with Agrobacterium produced the putative transgenic shoots on medium with 5mg/L bialaphos treatment after 3-4 weeks. Second selection was performed by transferring the shoot tips of putative transgenic to medium containing 20mg/L of bialaphos. The shoot tips grew well on the second selection medium, indicating the production of successful transgenic plants. But normal shoots were dead in same cytotoxic medium. Incorporation of the PAT gene into transgenic potatos were confirmed by PCR analysis of DNA and Southern hybridization. These results show that the PAT gene can serve as a selectable marker and herbicide resistant genes for transformation of potato.

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Effects of Dietary Protein Level and Phase Feeding Regimen on Growth Performance, Carcass Characteristics and Pork Quality in Growing-finishing Pigs

  • Choi, Jae-Young;Shinde, Prashant;Jin, Zheng;Kim, Jin-Soo;Chae, Byung-Jo
    • Journal of Animal Science and Technology
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    • v.52 no.3
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    • pp.205-212
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    • 2010
  • Two experiments were conducted to study the effects of dietary protein level and phase feeding regimens on performance, carcass characteristics and pork quality in growing-finishing pigs. In both experiments, growing pigs (n=90; barrows; Landrace $\times$ Yorkshire $\times$ Duroc) were allotted to 3 treatments. Each treatment had 3 replicate pens comprising 10 pigs in each. In Exp. 1, pigs (average body weight $25.06{\pm}0.57\;kg$) were fed diets having low (17.50-14.50-12.51%), medium (19.49-17.50-13.87%) or high (22.47-19.49-16.36%) CP concentration (LP, MP and HP diets, respectively) diets during grower-finisher I-finisher II phases, respectively. Feeding of MP diet resulted in higher (P<0.05) ADG and better FCR than pigs fed LP diet. Pigs fed HP diet had lower (P<0.05) pork color and marbling score. Meat of pigs fed LP diet had higher (P<0.05) Thiobarbituric acid reactive substances (TBARS), while meat of pigs fed HP and LP diets had greater drip loss than pigs fed MP diet. In addition, meat from pigs fed LP diet was darker while meat of pigs fed HP and MP diets was paler and redder. In Exp. 2, pigs (average body weight $10.40{\pm}0.61\;kg$) were subjected during 126 days to three or four phase regimes of different duration. The treatments T1 and T2 were subjected to four phase feeding regimens (grower I, grower II, finisher I and finisher II) for 22, 42, 32 and 30 days (T1) and 40, 40, 25 and 21 days (T2), respectively, while T3 was subjected to three phase feeding regimen (grower I, grower II and finisherI) for 40, 56 and 30 days, respectively. Duration of phase feeding regimens had no effect (P>0.05) on the performance, carcass traits and meat quality of pigs. These results suggest that both high and low levels of CP have a negative effect on performance and pork quality; however, alterations in the phase feeding regimens do not have any discernible effect on the performance and pork quality in growing-finishing pigs.

Nutrient Utilization, Body Composition and Lactation Performance of First Lactation Bali Cows (Bos sondaicus) on Grass-Legume Based Diets

  • Sukarini, I.A.M.;Sastradipradja, D.;Sutardi, T.;Mahardika, IG.;Budiarta, IG.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.12
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    • pp.1681-1690
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    • 2000
  • A study on energy and protein utilization, and milk production of Bali cows on grass-legume diets was carried out using 12 first lactation cows (initial BW $263.79{\pm}21.66kg$) during a period of 16 weeks starting immediately post calving. The animals were randomly allotted into 4 dietary treatment groups R1, R2, R3 and R4, receiving from the last 2 months of pregnancy onwards, graded improved rations based on a mixture of locally available grass and legume feed ad libitum. R1 contained on a DM basis 70% elephant grass (PP, Penisetum purpureum) plus 30% Gliricidia sepia leaves (GS), R2 was 30% PP plus 55% GS supplemented with 15% Hibiscus tilliactus leaves (HT, defaunating effect), R3 and R4 were 22.5% PP+41.25% GS+11.25% HT+25% concentrate, where R3 was not and R4 supplemented with zinc di-acetate. TDN, CP and zinc contents of the diets were 58.2%, 12.05% and 18.3 mg/kg respectively for R1, 65.05%, 16.9% and 25.6 mg/kg respectively for R2, 66.03%, 16.71% and 29.02 mg/kg respectively for R3 and 66.03%, 16.71% and 60.47 mg/kg respectively for R4. Milk production and body weight were monitored throughout the experimental period. In vivo body composition by the urea space technique validated by the body density method and supported by carcass data was estimated at the start and termination of the experiment. Nutrient balance and rumen performance characteristics were measured during a balance trial of 7 days during the 3rd and 4th week of the lactation period. Results indicated that quality of ration caused improvement of ruminal total VFA concentration, increments being 52 to 65% for R2, R3 and R4 above R1, with increments of acetate being less (31 to 48%) and propionate being proportionally more in comparison to total VFA increments. Similarly, ammonia concentrations increased to 5.24 to 7.07 mM, equivalent to 7.34 to 9.90 mg $NH_3-N/100ml$ rumen fluid. Results also indicated that feed quality did not affect DE and ME intakes, and heat production (HP), but increased GE, UE, energy in milk and total retained energy (RE total) in body tissues and milk. Intake-, digestible- and catabolized-protein, and retained-protein in body tissues and milk (Rprot) were all elevated increasing the quality of ration. Similar results were obtained for milk yield and components with mean values reaching 2.085 kg/d (R4) versus 0.92 kg/d (R1) for milk yield, and 170.22 g/d (R4) vs 71.69 g/d (R1), 105.74 g/d (R4) vs 45.35 g/d (R1), 101.34 g/d (R4) vs 46.36 g/d (R1) for milk-fat, -protein, and -lactose, respectively. Relatively high yields of milk production was maintained longer for R4 as compared to the other treatment groups. There were no significant effects on body mass and components due to lactation. From the relationship $RE_{total}$ (MJ/d)=12.79-0.373 ME (MJ/d); (r=0.73), it was found that $ME_{m}=0.53MJ/kgW^{0.75}.d$. Requirement of energy to support the production of milk, ranging from 0.5 to 3.0 kg/d, follows the equation: Milk Prod. ($Q_{mp}$, kg/d)=[-2.48+4.31 ME($MJ/kg^{0.75}.d$)]; (r=0.6) or $Q_{mp}$=-3.4+[0.08($ME-RE_{body\;tissue}$)]MJ/d]; (r=0.94). The requirement for protein intake for maintenance ($IP_m$) equals $6.19 g/kg^{0.75}.d$ derived from the relationship RP=-47.4+0.12 IP; (r=0.74, n=9). Equation for protein requirement for lactation is $Q_{nl}$=[($Q_{mp}$)(% protein in milk)($I_{mp}$)]/100, where $Q_{nl}$ is g protein required for lactation, $Q_{mp}$ is daily milk yield, Bali cow's milk-protein content av. 5.04%, and $I_{mp}$ is metabolic increment for milk production ($ME_{lakt}/ME_{m}=1.46$).

Analysis of Surface EMG Power Spectrum and Muscle Fatigue Depending on the Variable of Neuromuscular Electrical Stimulation (표면근전도를 이용한 신경근 전기자극 치료변수에 따른 근피로도 분석)

  • Kim, Gi-Won;Kim, Junesun
    • The Journal of Korean Physical Therapy
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    • v.26 no.5
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    • pp.280-289
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    • 2014
  • Purpose: This study was conducted in order to determine the stimulation variables which should be considered when neuromuscular electrical stimulation (NMES) is applied for a muscle under the normal innervation to minimize muscle fatigue and increase force-generating ability. Methods: A total of 23 healthy men participated in the study and all subjects were randomly assigned to the 1:1 group, 1:3 group, 1:5 group, and control group with on-off ratio of NMES. The subjects performed a fatigue task, consisting of 10 times of isometric contraction sustained by NMES. NMES using Russian current stimulation was applied to muscle fatigue and divided into three sessions by pulse frequency (10 bps, 30 bps, 90 bps). The EMG was recorded using an MP 100 system from the quadriceps femoris muscle in four groups. Results: The differences of delta MdF and delta MF of between on-off ratio groups of 10 bps, 30 bps, and 90 bps pulse frequencies were very significant (p<0.05). According to the results of post hoc of 10, 90 bps, it was greater in the 1:1 group and the 1:3 group compared with the 1:5 group, and no fatigue was observed in the control group. In 30 bps, it was greater in the 1:1 group compared with 1:3, 1:5, and control group (p<.05). Conclusion: Among NMES variables to minimize muscle fatigue, the larger on-off ratio by pulse frequency showed the lower muscle fatigue. Therefore, on-off ratio needs to be great enough, and will be more efficient with the frequency 30 bps rather than of 10 bps and 90 bps.

Consumer Satisfaction Model for Digital Convergence Products (디지털컨버전스 제품에 대한 소비자만족모델)

  • Yoo, Hyun-Jung
    • Journal of Families and Better Life
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    • v.27 no.5
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    • pp.25-42
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    • 2009
  • This study examines the actual state of using digital convergence products by consumers, and analyzes the expectation that consumers have regarding digital convergence products and the actual satisfaction of the digital convergence products. In addition, it analyzes factors affecting the satisfaction with digital convergence products. First, mobile phones, which 99.4% of those surveyed had, cameras, which 90.6% had, and mp3s, which 71.8% had, were found to be necessities. Second, the ratio of owning digital convergence products was 79.5%, 48.1%, and 52.4%, respectively. In the case of a mobile phones, a digital convergence type is already common. Third, regarding the reason for purchasing digital convergence products, they chose convergence products because there was no product with a simple function that they wanted to have: 38.2% in terms of a mobile phone, 47.2% for a camera, and 28.2% for an mp3. This shows that a consumer's choice was infringed in a great deal at the back of remarkable quantitative growth in digital convergence products. Fourth, the analysis of the factor regarding the consumer's expectation on digital convergence products showed 3 kinds of factors, i.e., expectation of benefit, expectation of risk, and expectation of situation. Fifth, as a result of analyzing the structural model regarding the effect on satisfaction by a consumer's expectation and the outcome on digital convergence products, all the expectations and outcomes of benefit, risk, and situation did not have a direct effect on satisfaction. Only an indirect effect through inconsistency showed a statistical significance.

A Stochastic Serving MPP Selection Method for Increasing the Efficiency of a Wireless Mesh Network (무선메쉬망에서 효율 증대를 위한 확률적 접속 MPP 선정 기법)

  • Park, Jae-Sung;Lim, Yu-Jin
    • Journal of the Korea Society of Computer and Information
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    • v.14 no.6
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    • pp.83-90
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    • 2009
  • Since traffic is aggregated to a MPP that acts as an Internet gateway, if traffic load is not balanced among the MPPs in a WMN, the overall performance of a WMN becomes poor even though the total traffic load is far below the capacity of the WMN. Therefore, in this paper, we propose a stochastic load balancing scheme where each MP (Mesh Point) probabilistically selects its serving MPP according to the congestion levels of MPPs. Through extensive simulations using ns-2, we have verified that our scheme can stabilize a WMN fast when congestion occurs and reduce packet loss rate by distributing traffic load of a congested MPP to multiple MPPs in the inverse proportional to their congestion levels. Compared to queue-based load balancing scheme, our method can decrease network stabilization time by 34 seconds, and reduce packet loss rate by 7.6%. Since the proposed scheme can reduce network stabilization time by efficiently using network resource, it is expected to contribute to the reliable operation of a WMN.