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Effect of Filtrate of Loess Suspension on Growth and Quality of Soybean Sprouts (콩나물의 생장과 품질에 미치는 황토 지장수의 효과)

  • Kang, Jeng-Yeol;Kang, Sun-Chul;Park, Shin
    • Applied Biological Chemistry
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    • v.43 no.4
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    • pp.266-270
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    • 2000
  • Using the filtrate of loess suspension, we cultivated soybean sprouts and investigated its effect on growth and quality in soybean sprouts. In comparison with soybean sprouts cultivated by tap water, the soybean sprouts cultivated by the filtrate of loess suspension at $20^{\circ}C$ showed increases in its weight by 11.4% and length by 14.9%. When cultivated at $25^{\circ}C$, the soybean sprouts by filtrate of loess suspension also showed increases in its weight by 9.9% and length by 11.0%. We compared inorganic element contents and pH level between the filtrate of loess suspension and tap water. Contents of Ca, Mg, K, Na, Zn, Mn, and Cu did not show any difference, while only P was higher in the tap water. pH value did not show much difference either. Consequently, it seemed that inorganic element contents and pH in the filtrate of loess suspension did not give any effect on the growth of the soybean sprouts. And also there was no any significant difference in inorganic element and amino acid contents in two kinds of soybean sprouts. However in a sensory test, the color and overall acceptability of the soybean sprouts cultivated by the filtrate of loess suspension showed better than the soybean sprouts cultivated by the tap water.

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Purification and Characterization of the Lectins from Mushroom Flammulina velutipes (팽이버섯으로부터 Lectin의 정제와 특성)

  • Kim, Hyung-Suk;Son, Seung-Yeol;Hwang, Se-Young;Hong, Bum-Shik
    • Applied Biological Chemistry
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    • v.42 no.4
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    • pp.304-309
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    • 1999
  • Two Lectins, designated FVL-1 and FVL-2, were isolated and purified from the fruiting bodies of edible mushroom Flammulina veluripes using ammonium sulfate fractionation, ethanol treatment, DEAE-TOYPEARL ion-exchange column chromatography, and TSK-Gel HW-55F column chromatography. Specific activity increased 18 folds for FVL-1 and 7.9 folds for FVL-2 from ethanol treated sample. SDS-PAGE of FVL-1 and FVL-2 gave apparent molecular mass of 10.6 kDa and 37 kDa, respectively. FVL-2 agglutinated all type of human erythrocytes (A, B, AB, and O). However, FVL-1 agglutinated more human erythrocyte type O than type A, B, and AB. The hemagglutination activities of the FVL-1 were effectively inhibited by bovine submaxillary and porcine stomach mucins(BSM and PSM), fetuin, asialofetuin and cations, such as $Cu^{2+}$, $Mg^{2+}$, $Ca^{2+}$, $Mn^{2+}$ and $Fe^{2+}$. However, FVL-2 was not inhibited by any cations. The hemagglutination activities of the two lectins were not inhibited by the sugar, such as lactose, galactose and sugar derivatives. FVL-1 and FVL-2 were stable at pH $5{\sim}11$ and pH $4{\sim}7$, respectively. FVL-1 was stable below $55^{\circ}C$ and FVL-2 was below $45^{\circ}C$.

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Uncertainty, Self-Efficacy & Coping in Parents′ of Children with Cancer (암환아 부모의 질병에 대한 불확실성, 자기효능감 및 대처정도)

  • Oh Won Oak;Park Eun Sook
    • Child Health Nursing Research
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    • v.4 no.2
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    • pp.274-285
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    • 1998
  • Improvements in therapy have resulted in increasing numbers of children being successfully treated for cancer. However the agrressiveness of therapy & uncertainty about prognosis are associated with many adverse effects, psychological as well as physical for both the child & family. The purpose of this study were to measure the degree of perceived uncertainty, self-efficacy & coping, and then to examine the relationship between the perceived uncertainty, self-efficacy & coping in parents of children cancer patients. The subjects of this study consist of 140 parents with pediatric cancer, registered at pediatric cancer ward & Out Patient Department. Data was collected from July 1st to August 15th 1998. The tools used in this study were Mishel's the Parents' Perception of Uncertainty Scale (28 item, 4 likert scale), Shere's Self-Efficacy Scale (17 item, 5 likert scale) & Folkman & Lazarus Ways of Coping Checklist(34 items, 4 likert scale). Data was analyzed by t-test, Anova, Pearson Correlation coefficient. Results of this study are summerized as follows 1. Parents perceived their uncertainty to be slightly high(Mn 2.41). The degree of perceived uncertainty by the four components were followed as lack of clarity (2.60), unpredictability(2.59), ambiguity(2.51) & lack of information(1.90). The degree of perceived uncertainty of parents with pediatric cancer revealed to be influenced significantly by the family outcome, reliability about health care provider & perceived severity of illness. 2. The range of parents' self-efficacy was measured iron 35 to 85 point, so revealed slightly high. The degree of self-efficacy related to be Influenced signiicantly by the sequency of child birth, family religion & degree of perceived support. 3. The degree of parents' coping was measured slightly high (Mn 2.78). The degree of coping related to be influnced significantly by the sequency of child birth, number of sible & degree of perceived support. 4. parents' uncertainty was related inversely to the parents' self-efficacy(r=-.38, p<.001) & coping(r=-.26, p<.001). And also parents' self-efficacy was positively related to coping(r=.56, p<.001) From the above results, it can be concluded that predicting & controlling parents' uncertainty with children cancer are necessary to improve positive coping strategies. This information may be used as a foundation for developing nursing interventions to decrease perceived uncertainty & to foster self-efficacy & coping for parents with children cancer.

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Component(mineral) difference of Phellinus linteus on cultured to timber (기주식물에 따른 목질진흙버섯의 일반성분(무기물) 함량변화 I)

  • Lee, Soo-Won;Han, Ki-Won;Jang, Won-Cheoul;Lee, Dae-Jin;Jung, Dong-Sik;Lee, Byung-Eui
    • Journal of Mushroom
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    • v.3 no.3
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    • pp.100-104
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    • 2005
  • We investigated mineral difference from mushroom Phellinus linteus and Phellinus baumii on mulberry, oak and elm. ICP test, The major mineral components were K, Ca, and Fe, while Mg, Na, P, Cr, Mn, Cu and Zn were determined as micro mineral components. In case of P.linetus and P.baumii the component of K was shown as 699.0mg/100g and 630mg/100g, in oak, 340.7mg/100g and 314.6mg/100g in elm, and 311.6mg/100g and 311.6mg/100g in mulberry respectively. In Ca, mulberry fruiting body were shown about 2 times highest and expecially in case of Na was oak fruiting body were shown as 10 times highest as against the others sample groups. In micro mineral components, Zn component was shown very diffeence about sample groups. We concluded that the highest mineral component of K was shown in mushroom cultivated on oak of both P.linetus and P.baumii. As a results, mineral component has showed very difference on cultivated to timber. According to the results of mineral component we concluded that Phellinus linteus and Phellinus baumii has mineral component different depending on timber.

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Screening of Biodegradable Function of Indigenous Ligno-degrading Mushroom Using Dyes

  • Jang, Kab-Yeul;Cho, Soo-Muk;Seok, Soon-Ja;Kong, Won-Sik;Kim, Gyu-Hyun;Sung, Jae-Mo
    • Mycobiology
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    • v.37 no.1
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    • pp.53-61
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    • 2009
  • The process of biodegradation in lingo-cellulosic materials is critically relevant to biospheric carbon. The study of this natural process has largely involved laboratory investigations, focused primarily on the biodegradation and recycling of agricultural by-products, generally using basidiomycetes species. In order to collect super white rot fungi and evaluate its ability to degrade lingo-cellulosic material, 35 fungal strains, collected from forests, humus soil, livestock manure, and dead trees, were screened for enzyme activities and their potential to decolorize the commercially used Poly-R 478 dye. In the laccase enzymatic analysis chemical test, 33 white rot fungi and 2 brown rot fungi were identified. The degradation ability of polycyclic aromatic hydrocarbons (PAHs) according to the utilized environmental conditions was higher in the mushrooms grown in dead trees and fallen leaves than in the mushrooms grown in humus soil and livestock manure. Using Poly-R 478 dye to assess the PAH-degradation activity of the identified strains, four strains, including Agrocybe pediades, were selected. The activities of laccase, MnP, and Lip of the four strains with PAH-degrading ability were highest in Pleurotus incarnates. 87 fungal strains, collected from forests, humus soil, livestock manure, and dead trees, were screened for enzyme activities and their potential to decolorize the commercially used Poly-R 478 dye on solid media. Using Poly-R 478 dye to assess the PAHdegrading activity of the identified strains, it was determined that MKACC 51632 and 52492 strains evidenced superior activity in static and shaken liquid cultures. Subsequent screening on plates containing the polymeric dye poly R-478, the decolorization of which is correlated with lignin degradation, resulted in the selection of a strain of Coriolus versicolor, MKACC52492, for further study, primarily due to its rapid growth rate and profound ability to decolorize poly R-478 on solid media. Considering our findings using Poly-R 478 dye to evaluate the PAH-degrading activity of the identified strains, Coriolus versicolor, MKACC 52492 was selected as a favorable strain. Coriolus versicolor, which was collected from Mt. Yeogi in Suwon, was studied for the production of the lignin-modifying enzymes laccase, manganese-dependent peroxidase (MnP), and lignin peroxidase (LiP).

Examination of the Production of Extracellular $\alpha$-Amylase by Bacillus thuringiensis, 19 serotypes (Bacillus thuringiensis, 19 혈청형의 세포외 $\alpha$-Amylase 생산 검색)

  • 이건주;박동왕;이형환;이영주
    • Microbiology and Biotechnology Letters
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    • v.16 no.5
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    • pp.348-351
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    • 1988
  • The production of extracellular $\alpha$-amylase by Bacillus thuringiensis 19 serotypes was examined by the hydrolysis test of starch. Thirteen serotypes produced the amylase. B. thuringiensis serovar thuringiensis alesti, kurstaki, sotto, kenya, israelensis, morrisoni, entomocidus, tolworthi, thompsoni, toumanoffi, pakistani, and indiana produced tee enzyme. The amylase produced by B. thuringiensis serovar israelensis showed highest activity around pH 6.7 to 7.2 and 55$^{\circ}C$ to $65^{\circ}C$. The high production medium of the amylase was composed of 1% bactopeptone, 0.3% beef-extract, 0.3% yeast ex-tract, 0.5% NaCl, 0.3% $K_2$HPO$_4$, 0.1% KH$_2$PO$_4$, 0.2% Soluble Starch, 0.012% CaCl$_2$.2$H_2O$$_2$, 0.005% MnCl$_2$, and 0.03% MgCl$_2$.7$H_2O$. The highest production of the enzyme was observed at 4 hours culture in the soluble starch (0.6 units/$m\ell$) or glucose (0.43 units/$m\ell$) substrate.

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Electrode characteristics of $AB_2$ type hydrogen storage alloy modified by Cr, La addition and fluorination ($AB_2$계 수소저장합금의 전극특성에 미치는 Cr, La 첨가 효과 및 표면 불화처리 효과)

  • Chang I.;Lee B. H.;Cho W. I.;Jang H.;Cho B. W.;Yun K. S.
    • Journal of the Korean Electrochemical Society
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    • v.1 no.1
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    • pp.45-51
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    • 1998
  • [ $AB_2-type$ ] alloy, one kind of hydrogen storage alloys used as an anode of Ni-MH batteries, has large discharge capacity but has remaining problems regarding initial activation, cycle life and self-discharge. This study investigates the effects of Cr-addition and fluorination after La-addition on $Zr_{0.7}Ti_{0.3}V_{0.4}Mn_{0.4}Ni_{1.2}$, composition $AB_2-type$ alloy. EPMA and SEM surface analysis techniques were used and the crystal structure was characterized by XRD analysis. In addition, electrodes were fabricated out of the alloys and characterized by constant current cycling test, electrochemical impedance spectroscopy and potentiodynamic polarization. Cr-addition was found to be effective to cycle life and self-discharge but ineffective to initial activation due to formation of stable oxide film on surface. Fluorination after La-addition to the alloys improved initial activation remarkably due to formation of highly reactive particles on surface.

Vertical Flow Zeolite-Filled Reed Bed with Intermittent Feeding for Sewage Treatment (수직 흐름 제올라이트 갈대 여과상에 의한 생활하수 처리)

  • Seo, Jeoung-Yoon
    • KSBB Journal
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    • v.22 no.2
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    • pp.102-108
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    • 2007
  • A sewage was treated using a vertical flow zeolite-filled reed bed. The sewage from the student dormitory of Changwon National University was fed into the reed bed for 10 minutes every 6 hours at the hydraulic load of $314L/m^3{\cdot}$day. The filtering height of the reed bed was 100 em and the zeolite mixture was filled in the reed bed. The mixture consisted of the same volume of two types of zeolite: 0.5$\sim$1 mm and 1$\sim$3 mm in diameter. Annual average removal efficiency was 88 89.9%, $COD_{Cr}$ 86.1 %, $COD_{Mn}$ 81.2%, T-N 34.0%, $NH_4^+$-N 97.3% and T-P 34.6%. T-N of effluent was mostly $NO_3^-$-N and the concentration of $NO_2^-$-N in effluent was lower than 0.1 mg/L. All removal efficiencies did not show a remarkable seasonal change. The ranking of phosphorous fractions fixed to the zeolite in column test was Ca-P > Fe-P > reductant soluble Fe-P > occluded P > saloid P > AI-P at all depths of the filter. All phosphorous fractions except for AI-P reduced at deeper filter layer, while their content ratios increased at deeper filter layer. Organic matter content was the highest at the highest layer (0$\sim$5 cm from the top of the filter) and only small differences were observed at the deeper filter layer than 5 em from the top. Organic matter content increased at all depths of the filter with the operating time.

Charge-Discharge Characteristics of Lithium Metal Polymer Battery Adopting PVdF-HFP/(SiO2, TiO2) Polymer Electrolytes Prepared by Phase Inversion Technique (상반전 기법으로 제조한 PVdF-HFP/(SiO2, TiO2) 고분자 전해질을 채용한 리튬금속 고분자 2차전지의 충방전 특성)

  • Kim, Jin-Chul;Kim, Kwang-Man
    • Korean Chemical Engineering Research
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    • v.46 no.1
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    • pp.131-136
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    • 2008
  • Silica- or titania-filled poly (vinylidene fluoride-co-hexafluoropropylene)-based polymer electrolytes were prepared by phase inversion technique using N-methyl-2-pyrrolidone and dimethyl acetamide as solvent and water as non-solvent. The polymer electrolytes were adopted to the lithium metal polymer battery using high-capacity cathode $Li[Ni_{0.15}Co_{0.10}Li_{0.20}Mn_{0.55}]O_2$ and lithium metal anode. After the repeated charge-discharge test for the cell, it was proved that the cell adopting the polymer electrolyte based on the phase-inversion membrane containing 40~50 wt% silica showed the highest discharge capacity (180 mAh/g) until 80th cycle and then abrupt capacity fade was just followed. The capacity fade might be due to the deposition of lithium dendrite on the polymer electrolyte, in which the capacity retention was no longer sustainable.

A Study of Mechanical Properties With Variation of Heattreatments on HSLA Cast Steels Microalloyed With Nb, Ti, and V (Nb, Ti 및 V를 첨가한 HSLA 주강의 열처리 변화에 따른 기계적 특성 연구)

  • Park, Jae-Hyeon;Kim, In-Bae
    • Korean Journal of Materials Research
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    • v.10 no.11
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    • pp.760-769
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    • 2000
  • Mechanical properties of HSLA cast steels alloyed with 0.15% Nb, Ti or V were tested as variations of austenizing temperatures and tempering times. The test results are as follows. The hardness of HSLA cast steels austenized for 2hrs at 115$0^{\circ}C$ was shown the highest value regardless of alloying elements and then decreased as the temperature decreased below 110$0^{\circ}C$. The hardness of HSLA cast steels with 0.15% Ti austenized for 2 hrs at $1150^{\circ}C$ was higher than that of any other HSLA cast steels, and chich was mainly attributed to the relatively high amount of bainite, and solid solution hardening. Charpy impact energy of HSLA cast steels was comparable to the C-Mn cast steel except HSLA cast steels with 0.15% Ti austenized at 115$0^{\circ}C$. The hardness of HSLA cast steels austenized for 2 hrs at $1150^{\circ}C$ increased at a ten-minute tempering, and after that, the hardness kept almost sililar level except HSLA cast steels with 0.15% V.

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