• Journal of mucopolysaccharidosis and rare diseases
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• v.2 no.1
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• pp.1-4
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• 2016

Mucolipidosis type II (MLII; MIM#252500) and type III alpha/beta (MLIIIA; MIM#252600) very rare lysosomal storage disease cause by reduced enzyme activity of GlcNAc-1-phosphotransferase. ML II is caused by a total or near total loss of GlcNAc-1-phosphotransferase activity whether enzymatic activity in patient with ML IIIA is reduced. While ML II and ML III share similar clinical features, including skeletal abnormalities, ML II is the more severe in terms of phenotype. ML III is a much milder disorder, being characterized by latter onset of clinical symptoms and slower progressive course. GlcNAc-1-phosphotransferase is encoded by two genes, GNPTAB and GNPTG, mutations in GNPTAB give rise to ML II or ML IIIA. To date, more than 100 different GNPTAB mutations have been reported, causing either ML II or ML IIIA. Despite development of new diagnostic approach and understanding of disease mechanism, there is no specific treatment available for patients with ML II and ML IIIA yet, only supportive and symptomatic treatment is indicated.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.13 no.11
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• pp.2405-2411
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• 2009

MMSE, ZF and ML are the decoding mechanisms for V-BLAST system, and ML shows the best performance decoding the original signal among them. However, it has a problem that the computation complexity is increased exponentially according to the number of transmit antennas and transmit degrees. In this paper, we propose a low complexity linear ML detection algorithm having low computation complexity, then analyze the system performance in BER and computation complexity comparing with other algorithms. In the simulation, the BER performance of the proposed algorithm is superior than ZF and MMSE detection algorithms, and similar to ML detection algorithm. However, its computation complexity was 50% less than ML algorithm. From the results, we confirm that the proposed algorithm is superior than other ML detection algorithms.

• Journal of Environmental Health Sciences
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• v.8 no.2
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• pp.47-51
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• 1982

135 Raw milk samples corrected from 4 dairy plants before processing were held at 63$\circ$C for 30 minutes and 80$\circ$C for 10 minutes. Thermoduric mesophiles, psychrotrophs and thermophilic bacteria were estimated from the samples treated at 63$\circ$C for 30 minutes and aerobic spore formers from the samples treated at 80$\circ$C for 10 minutes. The results obtained were as follows. 1) The distribution of thermoduric mesophiles were $10^4 - 10^5$ cfu/ml (45.2%), $10^2 - 10^4$ cfu/ml (21.5%), $10^5$ cfu/ml over (20.7%), $10^2 - 10^3$ cfu/ml (8.9%) and $10 - 10^2$ cfu/ml (3.7%). 2) The distribution of thermoduric psychrotrophs were 10 - $10^2$ cfu/ml (40.2%), $10^2 - 10^3$ cfu/ml (29.5%), 10 cfu/ml below (22.3%) and $10^3 - 10^4$ cfu/ml (8.0%). Isolated psychrotrophs had the characteristics of Streptococcus, Micrococcus and Alcaligenes group. 3) The distribution of thermophilic bacteria were 10 cfu/ml below (91.9%), and 10 - $10^2$ cfu/ml (81.8%). 4) Aerobic spore formers counts were 10 cfu/ml below (27.4%), $10^3 - 10^4$ cfu/Illl (26.5%), $10^2 - 10^3$ cfu/ml (23.9%)and 10 - $10^2$ cfu/ml (22.1%).

• Journal of Periodontal and Implant Science
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• v.33 no.3
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• pp.415-437
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• 2003

Ginseng Radix(GR) had been used widely from oriental medicine and the effects of it have been investigated by many researchers. The purpose of present study was to investigate the effects of GR on the cell cycle progression and its molecular mechanism in human fetal osteoblast. The results were as follows. Increased cell proliferation was observed in cells exposed to 100 ng/ml, 10 ng/ml of GR-1 at 12 hours and 24 hours, 1 ${\mu}g$/ml of GR-1 at 48 hours, and 100 ${\mu}g$/ml, 10 ${\mu}g$/ml of GK-2 at 12 hours, all treatment groups of GR-2 at 24 hours(p<0.05). S phase and G1 phase was increased in the group of treated with 100 ng/ml of GR-1, with 10 ${\mu}g$/ml and 1 ${\mu}g$/ml of GR-2, with 100 ${\mu}g$/ ml and 10 ${\mu}g$/ml of GR-3 in the cell cycle analysis. The cell cycle regulation protein levels of Cyclin D1, Cyclin E, CDK 2. CDK 4 and CDK 6 were increased in the group of treated with 1 ${\mu}g$/ml and 100 ng/ml of GR-1, with 10 ${\mu}g$/ml and 1 ${\mu}g$/ml of GR-2, with 100 ${\mu}g$/ ml and 10 ${\mu}g$/ml of GR-3. On the other hand, p21 was decreased in the treatment group with 1 ${\mu}g$/ml and 100 ng/ml of GR-1, with 10 ${\mu}g$/ml and 1 ${\mu}g$/ml of GR-2, 10 ${\mu}g$/ml of GR-3, and p53 and p16 was decreased in the treatment group with 100 ng/ml of GR-1, 100 ${\mu}g$/ml and GR-3 and pRb was decreased in the all treatment groups except 1 ${\mu}g$/ml of GR-1. These results suggested that GR increases the cell proliferation and the cell cycle progression in human fetal osteoblast, which is linked to increased cell cycle regulation protein levels of Cyclin D1 , Cyclin E, CDK 2, CDK 4, CDK 6 and decreased cell cycle regulation protein levels of p21, pRb.

• Korean Journal of Animal Reproduction
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• v.13 no.2
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• pp.85-92
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• 1989

The study was carried out to find out the changes of the sex hormone concentrations in the blood plasma and antrum of follicular and lutein cystic ovaries of Holstein cows. The progesterone, estradiol-17$\beta$, testosterone, FSH and LH from samples of the blood plasma and antrum of cystic ovaries of cows assayed by radioimmunoassay method. The results of this study were summarized as follows : 1. The concentrations fo progesterone and estradiol-17$\beta$ in the blood plasmaat estrous and luteal phase were 0.95$\pm$0.18ng/ml, 11.45$\pm$3.12pg/ml and 4.25$\pm$0.27ng/ml, 6.27$\pm$0.82pg/ml respectively. The concentrations of progesterone and estradiol-17$\beta$ in the antrum fluid of follicles at estrous and luteal phase were 24.8$\pm$4.12ng/ml, 54.3$\pm$7.25pg/ml and 21.7$\pm$3.79ng/ml, 14.3$\pm$2.72pg/ml respectively, and showed significant changes among the estrous and luteal phase and blood plasma and antrum fluid of follicles. 2. The concentrations of progesterone, estradiol-17$\beta$, testosterone and LH in the blood plasma of follicular cystic ovareis of cows were 0.85$\pm$0.25ng/ml, 9.23$\pm$2.72pg/ml, 17.12$\pm$3.26pg/ml and 3.78$\pm$1.02mIU/ml respectively. And the concentrations of progesterone, estradiol-17$\beta$, testosterone and LH in the antrum fluid of follicular cystic ovareis of cows were 284$\pm$48.21ng/ml, 389$\pm$67.23ng/ml, 12.84$\pm$0.29ng/ml and 1.84$\pm$0.17mIU/ml respectively, and showed significant changes between in the blood plasma and antrum fluid of cystic ovaries. 3. The concentrations of progesterone, estradiol-17$\beta$, testosterone and LH in the blood plasma of lutein cystic ovaries of cows were 3.40$\pm$0.78ng/ml, 4.02$\pm$0.42pg/ml, 10.72$\pm$2.74pg/ml and 0.76$\pm$0.12mIU/ml respectively. And the concentrations of progesterone, estradiol-17$\beta$, testosterone and LH in the antrum fluid of lutein cystic ovareis of cows were 427$\pm$35.79ng/ml, 0.76$\pm$0.07ng/ml, 3.45$\pm$0.57ng/ml and 0.29$\pm$0.07mIU/ml respectively, and showed significant changes between the blood plasma and antrum fluid of cystic ovaries. 4. Accordingly, the diagnosis of follicular and lutein cystic ovareis of cows from progesterone, estradiol-17$\beta$ and LH levels in the blood plasma and antrum of cystic ovaries of cows is thought to be possible a diagnostic means.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.28 no.1B
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• pp.153-160
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• 2008

To estimate microbial contaminant loading discharged from diffuse sources, rainfall runoff of six rainfall events were monitored at three study watersheds of forestry and agricultural land use. Monitored indicator microorganism constituents were total coliform (TC), fecal coliform (FC), Escherichia coli (EC), and fecal streptococcus (FS). Soil loss during elevated flow rate caused higher suspended solid concentrations. Indicator microorganism concentrations were closely related with flow rate. TC event mean concentration (EMC) from unpolluted forestry was $5.3{\times}10^3CFU/100ml$, FC EMC was $1.4{\times}10^3CFU/100ml$, EC EMC was $1.1{\times}10^3CFU/100ml$, and FS EMC was $2.9{\times}10^2CFU/100ml$. From a watershed with agricultural-forestry land use, TC EMC was $1.7{\times}10^5CFU/100ml$, FC EMC was $8.5{\times}10^4CFU/100ml$, EC EMC was $8.9{\times}10^4CFU/100ml$, and FS EMC was $3.4{\times}10^4CFU/100ml$. Mixed land use of agricultural-forestry with bigger area, TC EMC was $1.9{\times}10^5CFU/100ml$, FC EMC was $9.6{\times}10^4CFU/100ml$, EC EMC was $7.0{\times}10^4CFU/100ml$, and FS EMC was $5.1{\times}10^4CFU/100ml$.

• Korean Journal of Food Science and Technology
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• v.10 no.2
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• pp.189-193
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• 1978

Effects of the special media on the mycelium growth in Agaricus campestris has been studied. The results are summarized as follows; 1. The mycelium growth of Agaricus campestris were considerably Stimulated on the Carrot (Dancus carota L.) basal medium which was added 4ml. of carrot extract, Cucumber(Cucumis sativus L.) basal medium added 3ml of cucumber extract, and Radish (Rhaphanus sativus L.) basal medium added 3ml. of radish extract during the culture for 144 hours. 2. The mycelium of Agaricus campestris on the media which was added the several kinds of vegetable extracts shows a lot of growth for 144 hours. The orders are as follows; Carrot basal medium(4ml/100ml)>Cucumber basal medium (3ml/100ml)>Radish basal medium (3ml/100ml)>Lettuce basal medium (2ml/100ml)>Cabbage basal medium (2ml/100ml). However, the Lettuce (Lactuca scariota L.) basal medium and the Cabbage (Brassica chinensis L.) basal medium among these five media are no significant differences.

• Journal of Food Hygiene and Safety
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• v.23 no.2
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• pp.163-168
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• 2008

This study investigated the anti-oxidative and anti-cancer activities by extracting of Yacon by fraction with organic solvents. The content of total phenol was the highest by 45.53% in ethyl acetate fraction and the results of electron donating abilities were 65.20% ($100\;{\mu}g/ml$), 91.81% ($500\;{\mu}g/ml$) and 95.06% ($1000\;{\mu}g/ml$) and those of nitrite scavenging abilities were 11.71% ($100\;{\mu}g/ml$), 36.81% ($500\;{\mu}g/ml$), 59.70% ($1000\;{\mu}g/ml$) in ethyl acetate fraction, which were higher concentration than the control group. Xanthine oxidase inhibitory activities were 23.74% ($100\;{\mu}g/ml$) and 43.41% ($500\;{\mu}g/ml$) in low concentration, which showed higher activities than the control group. After measuring the growth inhibitory activities for cancer cells, it has been found that hexane fraction had the highest growth inhibitory activities by 23.75% ($10\;{\mu}g/ml$), 34.67% ($50\;{\mu}g/ml$), 54.21% ($100\;{\mu}g/ml$) for SNU-1. Meanwhile, hexane fraction demonstrated high growth inhibitory activities by 41.38% ($10\;{\mu}g/ml$), 50.53% ($50\;{\mu}g/ml$), 60.91% ($100\;{\mu}g/ml$) and butanol fraction had 17.05% ($10\;{\mu}g/ml$), 43.87% ($50\;{\mu}g/ml$), 62.99% ($100\;{\mu}g/ml$) for HeLa. Therefore, it has been believed that there were peculiar properties of anti-oxidative and anti-cancer activities depending on each fraction. Furthermore, it has been expected that further studies on the development of functional food and concrete components depending on the components of each fraction to be conducted.

• Korean Journal of Agricultural Science
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• v.11 no.2
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• pp.233-243
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• 1984

The present study was carried out to determine the changes of the sex hormone levels in serum throughout the estrous cycle and the gestation period on the Landrace gilts. The blood samples were taken from the vein of six gilts. LH, FSH, prolactin, progesterone, $estradiol-17{\beta}$ and cortisol in serum were analyzed by the radioimmunoassay methods. The results obtained on this study were summarized as follows; 1. The age at puberal estrus was 179.5 days, the weight at puberal estrus was 88.2kg, the length of estrous cycle was 21.3days, the gestation length was 114days and the litter size was 9.5 head in the Landrace gilts. 2. During the estrous cycle, the serum LH and prolactin concentrations were below 1.56mIU/ml and 2.4ng/ml, respectively, under the limit of detection of the assay. The FSH concentrations ranged from 1.50 to 2.20mIU/ml for day 6~15 after the estrus and they were below 1.25mIU/ml from day 3 to day + 3, with day 0 being the first day of the estrus. 3. Progesterone concentrations were 1.90ng/ml at day 0 of the estrus and increased about 13.1ng/ml at day 3 of the estrus, and reached peak levels at day 9. $Estradiol-17{\beta}$ concentrations were below 27.2pg/ml throughout the luteal phase, and reached about 27.2pg/ml at day 0 and day 18. Cortisol concentrations reached peak levels at dey 0 and ranged from 24.65 to 28.57ng/ml throughout the luteal phase. 4. During the gestation period, the concentrations of LH, FSH and prolactin ranged of 3.10~4.37mIU/ml, 1.30~1.80mIU/ml and 2.60~6.70ng/ml, respectively. 5. Progesterone concentrations declined from 38.90~16.85ng/ml throughout the pregnancy to 1.90ng/ml at the time of parturition. $Estradiol-17{\beta}$ concentrations increased from 27.20pg/ml at 15 days after the pregnancy to 620.17pg/ml at the time of parturition. Cortisol concentrations reached peak levels at the time of parturition and ranged from 13.58 to 22.31ng/ml throughout the pregnancy.

• Journal of dental hygiene science
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• v.2 no.1
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• pp.31-38
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• 2002

This study was to investigate the antibacterial effect of 63 species herbal medicine extracts on S. mutans growth by paper disc method and then examined the effects of S. mutans growth by some selected herbal medicines. Antibacterial effect of 63 species medicinal herb extracts on S. mutans was superior to Lycopi herba, Scutellariae radix in regular order and antibacterial activity(20 mg/ml concentration) through paper disc method were measured 6.3 mm and 3.5 mm, respectively. The growth of S. mutans in control medium was the highest at 8hrs, while that of medicinal herbs extract added-medium(2 mg/ml) was at 16 hrs. The pH values of the control media and media supplemented with Lycopi herba and Scutellariae radix extract were 5.08, 5.80, and 5.74 at 8 hrs, respectively. In the change of total carbohydrate of the control media and supplemented with Lycopi herba and Scutellariae radix extract were 0.81 mg/ml, 1.70 mg/ml and 1.84 mg/ml at 8 hrs, respectively. In the change of proteins of the control media and supplemented with Lycopi herba and Scutellariae radix extract were 8.39 mg/ml, 9.52 mg/ml and 9.28 mg/ml at 8 hrs, respectively. The polysaccharide contents of the control media and supplemented with Lycopi herba and Scutellariae radix extract were 300 mg/100 ml, 200 mg/100 ml and 220 mg/100 ml at 8hrs, respectively.