• Korean Journal of Veterinary Service
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• v.39 no.4
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• pp.227-230
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• 2016

Mycobacterium avium subspecies paratuberculosis (MAP) causes Johne's disease (JD) in ruminants. This is the first large scale report to estimate the herd-level prevalence of antibodies against MAP by using an ELISA to detect antibodies in bulk tank milk (BTM) samples. The samples were collected from January 2011 to November 2011, from 636 herds of the dairy farms in the Gyeonggi and Chungbuk areas of Korea. The overall apparent prevalence of MAP antibody-positive herds was 8.5%, and regional prevalence were 32/440 (7.3%) and 22/196 (11.2%) of dairy farms in the Gyeonggi and Chungbuk areas, respectively. The results did not differ significantly by region. While we have determined the prevalence rate of MAP in the Gyenoggi and Chungbuk areas in this study, there is a continuing need for well-designed studies to calculate the prevalence of MAP in dairy herds based on culture and molecular findings.

• Journal of Microbiology and Biotechnology
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• v.21 no.12
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• pp.1352-1355
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• 2011

Forty-one Enterococcus faecalis (E. faecalis) isolates from feces of pigs and chickens in Korea were screened for the presence of virulence factors. Gelatinase activity (85.4%, 35/41) was the more commonly observed phenotype of virulence in E. faecalis, compared with hemolytic activity (12.2%, 5/41). Thirty-one of 35 (88.6%) gelatinase-positive E. faecalis isolates harbored the gelE and fsrABC genes. A gene encoding for the enterococcal surface protein (Esp) was detected in 24.4% (10/41) of the isolates. All beta-hemolysin-producing isolates harbored the esp gene.

• Korean Journal of Veterinary Service
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• v.35 no.3
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• pp.169-174
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• 2012

Japanese encephalitis virus (JEV) is one of the main causes of viral encephalitis in human and animals. For over 30 years, a live attenuated JEV vaccine strain has been used in the veterinary field, and it is required to conduct quality evaluation studies on the commercial vaccines. For the quality control of live attenuated JEV vaccine, we investigated the nucleotide sequence similarity of prME gene derived from five JEV vaccines commercially available in pigs in Korea. The Vero cells infected with JEV vaccines showed specific cytopathic effect, which was characterized by rounding and detached cells. In the phylogenetic analysis, all of the vaccine strains showed a close relationship with the original vaccine seed strain (Anyang 300) and clustered into the genotype 3. In comparison of the nucleotide and deduced amino acid sequences of prME genes with the original strain, all JEV vaccine strains showed high amino acid similarity ranging from 98.9% to 99.5%, but had several point mutations, probably due to high mutation rates of viral RNA polymerase by several virus passages. Even though the current JEV vaccine strains have been maintained and produced for a long period of time, the genetic characterization of them have been rarely changed. However, since the mid 1990's, molecular epidemiology of JEV has been changed sharply from genotype 3 to genotype 1 in Korea, further studies on new vaccine strains to genotype 1 is required for more effective prevention in the field.

• Korean Journal of Breeding Science
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• v.43 no.4
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• pp.322-329
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• 2011

Microsatellite markers were utilized to investigate genetic diversity among 70 Korean barley varieties (Hordeum vulgare). Ninety nine microsatellite primer pairs were screened for 9 varieties. Twenty primer pairs showed highly polymorphic. The relationship between markers genotypes and 70 varieties was analyzed. A total of 124 polymorphic amplified fragments were obtained by using 20 microsatellite markers. Two to nine SSR alleles were detected for each locus with an average of 6.2 alleles per locus. Average polymorphism information content (PIC) was 0.734, ranging from 0.498 to 0.882. A total of 124 marker loci were used to calculate Jaccard's distance coefficients for cluster analysis using UPGMA. Clustering group was divided 2 groups corresponding to 2-rowed and 6-rowed barley varieties. The phenogram was discriminated all varieties by markers genotypes. These markers may be used wide range of practical application in variety identification and genetic purity assessment of barley.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.41 no.5
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• pp.399-404
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• 2008

This paper proposes revisions in the law governing assessments of the environmental impact of marine projects. In particular, we suggest strengthening the consultative role of the Ministry for Food, Agriculture, Forestry and Fisheries (MIFAFF), the agency responsible for regulating coastal area utilization and development, in order to improve the system by which fishery resources are managed, thus preventing negative environmental impact. Moreover, such an improved impact assessment statement would include reasonable evaluations of the dispersion of pollutants, such as the suspended solids generated by construction projects, as well as of the dredging, dumping and sand mining involved in coastal area reclamation. Thus, public confidence in the latter would be increased by the development and implementation of standardized and consistent guidelines addressing environmental research, simulation processes and evaluations of data.

• Korean Journal of Plant Resources
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• v.22 no.6
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• pp.571-583
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• 2009

Lam Dong Province of Vietnam has an exceptional diversity of orchids and herbs. At least 920 herb species are widely mentioned in Vietnamese traditional medicinal literature and over 239 Lam Dong wild orchid species are used for attention under the Conservation Vietnamese wild orchids projects. In collaboration with Department of Plant Biotechnology, Dongguk University - South Korea working under supported ODA funds from Korea government (MIFAFF), we will consider how to collecting and preserving these plants in situ or conservating in vitro as a genetic resource.

• Food Science of Animal Resources
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• v.30 no.6
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• pp.918-922
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• 2010

Due to the large amount of beef imported from the USA to Korea, Korean consumers have become increasingly interested in the country of origin since it can affect market prices. Previously, Bos indicus and Bos taurus-specific markers were developed for the purpose of cattle breed identification, specifically discrimination of Australian beef. In this study, six SNP markers derived from Illumina 50K bovine SNP chip data were used for the discrimination between Korean cattle (Hanwoo) and imported beef from USA. PCR-RFLP genotyping methods were also developed, which indicates that these markers can be applied relatively easily compared to other markers. Taking into account a discrimination rate of 55% based on MC1R marker between Hanwoo and imported beef from USA, two additional markers, SNPs 23803 and 34776, were ideal and resulted in probability of identification of 0.942 and probability of misjudgment of 0.03. Therefore, the markers developed in this study can greatly contribute to the correct discrimination between beef from USA and Hanwoo beef.

• The Journal of Fisheries Business Administration
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• v.41 no.2
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• pp.153-174
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• 2010

This study is aimed to analyze the establishing of fisheries management consulting system by case study of similar organizations. For a gradual extension of new technology of fisheries, National Fisheries Research and Development Institute(NFRDI) has launched fisheries consulting project including management in 2010. Fisheries management consulting needs practical and theoretical background regarding its standardization of process, systematization. To achieve this, fisheries management consulting system has to follow farm management consulting system by means of benchmarking. In order to analyze farm management consulting system, I investigated its role organizations(MIFAFF, RDA, KFRI), farm consulting guide. And also, it was conducted to investigated establishing of fisheries management consulting system through development of standard management consulting table and consulting indicators. The research results showed that the realm of fisheries management consulting should be redefined and systematized apposite to the case of fisheries actual conditions. In recent years, to enhance improvement of the fisheries income, there is need to be focused on the efficient utilization of fisheries management consulting system. It is expected that fisheries management consulting system be main issue in fisheries industry.

• Journal of Korean Society of Rural Planning
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• v.19 no.1
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• pp.55-70
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• 2013

Since 2004, Ministry for Food, Agriculture, Forestry and Fisheries(MIFAFF) has been carrying out comprehensive rural village development project as the core one of Rural Area Development Schemes in Korea. Up to now, 56 projects had been completed after having with 5-year plan implementation period, on which it is possible to try post-project evaluation works. This study aims to propose a rational income-generation works model of comprehensive rural village development project, based on fulfillment checking results of their statutory stepwise working procedures specified in the project guidelines and their interim/post project performance analysis results in 56 project areas above mentioned. In comprehensive rural village development project, income-generation works generally have both quantitative and qualitative effects to rural communities; the former is focussed on income increasement of villagers, while the latter on community revitalization through job creation and spill-over effects of other linked community activities. So, the research works of this study focussed on these two comprehensive effects above mentioned.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.48 no.4
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• pp.352-359
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• 2012

For rebuilding and managing fish stocks and fisheries, the biological and socioeconomic evaluations on fisheries management regulations are important. This study aimed to estimate the economic effectiveness of different mesh sizes in the yellow croaker gillnet fishery. In particular, by comparing economic effects of mesh sizes, 50mm and 52mm, it aimed to provide the economic validity for increasing mesh sizes. Analytical results showed that the fishing revenue was higher in the vessels using a mesh size over 52mm than that in the vessels using a mesh size less than 50mm. In addition, the effect of fishing cost reduction was also larger in the vessels using a mesh size over 52mm than that in the vessels using a mesh size less than 50mm.