• Title/Summary/Keyword: MICs

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Study on Activities of the Essential Oil from Elsholtzia ciliata against Some Antibiotic-Susceptible and -Resistant Strains of Pathogenic Bacteria (향유 지상부의 정유의 수종 항생제 내성 병원성 균주에 대한 억제효과)

  • Shin, Seung-Won
    • YAKHAK HOEJI
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    • v.54 no.2
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    • pp.122-125
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    • 2010
  • The essential oil fraction was extracted from the aerial parts of the plant by steam distillation method and its composition was analyzed by GC-MS (gas chromatography-mass spectrometry) which led to the identification of 43 compounds. Dehydroelsholtzia ketone (56.81%) and elsholtzia ketone (30.05%) were identified as the predominant components of this oil. The antibacterial activities of the essential oil fraction were assessed by micro-dilution tests against antibioticsusceptible and -resistant strains of Streptococcus pneumoniae, Staphylococcus aureus, Salmonella enteritidis, and S. typhimurium. The oil inhibited most of the tested strains significantly resulting MICs (minimum inhibiting concentrations) between 2 mg/ml and >16 mg/ml. In most cases of this study Streptococcus pneumoniae and Staphylococcus aureus showed higher sensitivity to this oil than Salmonella strains.

Prevalence and Antibiotic Susceptibility of Vancomycin-Resistant Enterococci in Chicken Intestines and Fecal Samples from Healthy Young Children and Intensive Care Unit Patients

  • Kim, Shin-Moo;Shim, Eun-Sook;Seong, Chi-Nam
    • Journal of Microbiology
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    • v.39 no.2
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    • pp.116-120
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    • 2001
  • The prevalence resistance genotype and antibiotic susceptibility of vancomycin-resistant enterococci (VRE) were determined. Prevalence of VRE in chickens, healthy children and intensive care unit (ICU) patients was 43.0%, 12.7% and 24.1%, respectively. Forty out of 56 isolates from chicken intestines were identified as Enterococcus faecium, and 12 were E. faecalis. All the isolates contained the vanA gene. Nine out of 13 VRE isolates from patients and two out of 21 from healthy young children were identified as E. faecium. The resistance types of E. faecium, E. gallinarium and E. casseliflavus were VanA, VanCl, and VanC2, respectively. The minimum inhibitory concentrations (MICs) of E. faecium, E. gallinarium, and E. casseliflavus to vancomycin were 512,8 and 4 g/ml, respectively. Specifically, E. faecium isolates were resistant to most of antibiotics except ampicillin and gentamicin. This is the first report of high VanA type VRE prevalence in nonhospitalized young children in Korea.

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Effect of Heat Treatments on the Antimicrobial Activities of Garlic (Allium sativum)

  • Kim, Jeong-Youn;Lee, Young-Chun;Kim, Keun-Sung
    • Journal of Microbiology and Biotechnology
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    • v.12 no.2
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    • pp.331-335
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    • 2002
  • Aqueous extracts of garlic (Allium sativum) preparation were prepared after the samples were exposed to various heat treatments. A quantitative assessment of antimicrobial activities was carried out by determining the minimum inhibitory and microbicidal concentrations (MICs and MMCs) of the various extracts against some selected bacteria and fungi. The antimicrobial activity of garlic decreased as the heating temperature increased. This fact implies that alliinase may be the most critical rate-determinant to produce the activity when garlic is heated.

A Triple Band Deep-Tissue Antenna for Biomedical Implants (심부 조직 인체 삽입용 세 가지 밴드 안테나)

  • Yoo, Hyoungsuk
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.66 no.2
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    • pp.383-386
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    • 2017
  • In this study, we introduce a triple-band flexible implantable antenna that is tuned by using a ground slot in three specific bands, namely Medical Implanted Communication Service (MICS: 402-405 MHz) for telemetry, the midfield band (lower gigahertz: 1.45-1.6 GHz) for Wireless Power Transfer (WPT), and the Industrial, Scientific and Medical band (ISM: 2.4-2.45 GHz) for power conservation. This antenna is wrapped inside a printed 3D capsule prototype to show its applicability in different implantable or ingestible devices. The telemetry performance of the proposed antenna was simulated and measured by using a porcine heart. From the simulation and measurement, we found that use of a ground slot in the implantable antenna can improve the antenna performance and can also reduce the Specific Absorption Rate (SAR).

Nano-scale high-accuracy displacement measurement using the Michelson laser interferometer controlled with a feedback circuit (되먹임 회로로 제어하는 Michelson 레이저 간섭계를 이용한 Nano-scale 미세변위 측정)

  • Ahn, Seong-Joon;Oh, Tae-Sik;Ahn, Seung-Joon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.8 no.5
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    • pp.1007-1012
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    • 2007
  • A novel Michelson interferometer controlled with a feedback circuit(MIFC) has been developed and its performance has been evaluated. This new interferometer can measure the displacement of the sample by directly reading the feedback bias applied to the PZT whose piezoelectric characteristic is known. The experimental result showed that the step height the silicon membrane measured by using MIFC was actually same with the value measured by SEM, which confirms that MICS is an easy and accurate method for the nano-scale displacement measurement.

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Activity of Essential Oil from Mentha piperita against Some Antibiotic-Resistant Streptococcus pneumoniae Strains and Its Combination Effects with Antibiotics

  • Choi, Sung-Hee;Shin, Seung-Won
    • Natural Product Sciences
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    • v.13 no.2
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    • pp.164-168
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    • 2007
  • To investigate natural antibiotics from plant essential oils and to evaluate their synergism with current antimicrobial drugs in inhibiting antibiotic-resistant strains of Streptococcus pneumoniae. The minimal inhibitory concentrations (MICs) of eleven plant essential oils and their main components were established for two antibiotic-susceptible and two antibiotic-resistant strains of S. pneumoniae, using broth microdilution tests. Potential synergism with oxacillin, norfloxacin, or erythromycin was evaluated using a checkerboard microtitre assay. Among the tested oils, Mentha piperita oil and its main component, menthol, exhibited the strongest inhibitory activities against all of the tested strains. The activity of antibiotics against antibiotic-resistant strains of S. pneumoniae was enhanced significantly by combination with Mentha piperita oils and its main component, menthol. In conclusion, the combination Mentha piperita essential oil or menthol with antibiotics could be used to reduce the effective dose of antibiotic and to modulate the resistance of S. pneumoniae strains.

Antimicrobial Susceptibility of Bordetella bronchiseptica Isolates from Pigs (돼지에서 분리한 Bordetella bronchiseptica의 항생제 감수성)

  • 조정곤
    • Journal of Veterinary Clinics
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    • v.15 no.1
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    • pp.30-35
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    • 1998
  • Susceptibility or resistance of the Bordetella bronchiseptica was determined by using the broth microdilution method. Each Bordetella bronchiseptica was tested fur sutraceptluility with 7 different antimicrobial agents.4 high prevalence of resistance (greater than 80 %) was found when Bordetella bronchiseptica was tested with amikacin (AK), ciprofloxacin ILrJl gentamlcin IGAgf, kanamycin (Kbf) and tobramycin (TM). However, the Bordetella bronchiseptica were sensitive to chloramphenicol (CP) and tetracycline (TC). Three different combination of drug resistance were observed : AM-CF-GM-TM (1 strain), AM-CF-GM- KM-TM (45 strains), and AM-CF-GM-KM-TM-CP (3 strains). The MICs against Bordetella bronchiseptica were 0.13 to >2.00 for TC, 4 to >648 for CP, 8 to >128 for CF 16 to >256 for GM, 16 to >256 for TM, 32 to >256 for KM and 64 to 256 for AM.

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Antimicrobial Resistance Patterns of Vancomycin-Resistant Streptococcus equinus Isolated from Animal Foods and Epidemiological Typing of Resistant S. equinus by Microbial Uniprimer Kit

  • Choi, Sung-Sook;Lee, Jin-Woo;Kang, Byoung-Yong;Ha, Nam-Joo
    • Archives of Pharmacal Research
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    • v.26 no.8
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    • pp.638-643
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    • 2003
  • Raw milk samples, and cow and chicken intestines were tested to isolate vancomycin-resistant, gram-positive bacteria. From these samples, we isolated seven vancomycin-resistant Streptococcus equinus, two vancomycin-resistant viridans Streptococcus and two vancomycin-resistant Enterococcus faecium. The MICs of several antibiotics, including vancomycin, against these strains were tested. Seven isolates of S. equinus showed high level resistance to vancomycin and teicoplanin (>100 $\mu$ g/mL). The cell wall thickness of these strains was compared with that of the sensitive strain by TEM and no differences were obserbed between these strains. We compared the strains of vancomycin-resistant Streptococcus equinus using PCR with Microbial Uniprimer Kit. We concluded that it is necessary to combine other methods in order to cluster and identify all isolates of S. equinus.

Study on antifungal activity of herb oils against Trichophyton spp

  • Shin, Seung-Won;Kim, Ji-Hyun;Lim , Sook;Pyun, Mi-Sun
    • Proceedings of the PSK Conference
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    • 2002.10a
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    • pp.384.1-384.1
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    • 2002
  • The antifungal activities of the essential oils from Citrus borgamia. Ciderus atlantica. Cymbopogon ditratus, Eucalyptus globulus. Juniperus communis. Lavandula angustifolia. Melaeuca aterinfolia. Pelargonium graveolens. Pogestemon patchouli. Rosmarinus officinalis. Styrax tonkinensis. and Thymus vulgaris, which are recommended for the treatment of microbial infections in aromatherapy and complementary medicines. were tested against Trichophyton spp. The activities were measured by broth dilution method and disk diffusion assay. As the results, most of the test oils inhibited growth of T. tonsurans. T.mentagrophytes. T. ferugineum. and T. rubrum. Eapecially, the essential oils from C. atlantica. C. ditratus. e. globulus, and P. graveolens showed the strongest activity among the tested herb oils showing MICs between < 0.09 and 0.39 mg/ml.

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High-level mupirocin resistance in Gram-positive bacteria isolated from diseased companion animals

  • Sum, Samuth;Park, Hee-Myung;Oh, Jae Young
    • Journal of Veterinary Science
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    • v.21 no.3
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    • pp.40.1-40.8
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    • 2020
  • The purpose of this study was to investigate the high-level mupirocin resistance (HLMR) in Gram-positive bacteria isolated from companion animals. A total of 931 clinical specimens were collected from diseased pets. The detection of mupirocin-resistant bacteria and plasmid-mediated mupirocin resistance genes were evaluated by antimicrobial susceptibility tests, polymerase chain reactions, and sequencing analysis. Four-hundred and six (43.6%) bacteria were isolated and 17 (4.2%), including 14 staphylococci and 3 Corynebacterium were high-level mupirocin-resistant (MICs, ≥ 1,024 ug/mL) harboring mupA. Six staphylococci of HLMR strains had plasmid-mediated mupA-IS257 flanking regions. The results show that HLMR bacteria could spread in veterinary medicine in the near future.