• 한국습지학회지
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• 제16권3호
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• pp.315-325
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• 2014

월악산국립공원에 발달되어 있는 신갈나무림에서 2012년 5월부터 2013년 4월까지 유기탄소 분포와 순환을 통한 생태계 서비스 가치를 파악하였다. 지상부와 지하부 생물량에 분포되어 있는 유기탄소량은 각각 81.94 및 20.53 ton C/ha 이었으며, 낙엽층과 토양의 유기탄소량은 각각 6.49 ton C $ha^{-1}$, 141.23 ton C $ha^{-1}$ $50cm-depth^{-1}$로 조사되였다. 조사지 신갈나무림의 전체 유기탄소량은 250.19 ton C $ha^{-1}$이었으며, 이중 41.0%가 식물체에 분포하였다. 신갈나무림의 전체 유기탄소량을 원화로 환산하면 약 527만원 $ha^{-1}$의 가치를 갖는 것으로 추정되었다. 조사기간 동안 토양호흡을 통하여 방출되는 탄소량은 7.31 ton C $ha^{-1}yr^{-1}$으로 이중 미생물호흡과 뿌리호흡을 통해 방출되는 탄소량은 각각 3.58, 3.73 ton C $ha^{-1}yr^{-1}$이었다. 유기탄소 순 생산량과 미생물호흡량의 차이로 추정했을 때 본 신갈나무림에서 연간 대기로부터 흡수하는 순 유기탄소는 1.61 ton C $ha^{-1}yr^{-1}$로서, 이를 원화로 환산하면 약 33,000원 $ha^{-1}$의 가치를 갖는 것으로 추정되었다.

• 한국습지학회지
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• 제17권4호
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• pp.332-338
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• 2015

월악산국립공원에 발달되어 있는 소나무림에서 2013년 1월부터 2013년 12월까지 유기탄소 분포와 순환을 통한 생태계 서비스 가치를 평가하였다. 지상부와 지하부 생물량에 분포되어 있는 유기탄소량은 각각 32.17 및 8.04 ton C $ha^{-1}$이었으며, 낙엽층과 토양의 유기탄소량은 각각 5.55 ton C $ha^{-1}$ 및 58.62 ton C $ha^{-1}$ 50cm-$depth^{-1}$로 조사되었다. 조사지 소나무림의 전체 유기탄소량은 104.38 ton C $ha^{-1}$이었으며, 이중 37.9%가 식물체에 분포하였다. 소나무림의 전체 유기탄소량을 원화로 환산하면 약 1,044 만원 $ha^{-1}$의 가치를 갖는 것으로 추정되었다. 조사기간 동안 토양호흡을 통하여 방출되는 탄소량은 4.44 ton C $ha^{-1}yr^{-1}$으로 이중 미생물호흡과 뿌리호흡을 통해 방출되는 탄소량은 각각 2.18 및 2.27 ton C $ha^{-1}yr^{-1}$이었다. 유기탄소 순 생산량과 미생물호흡량의 차이로 추정했을 때 본 소나무림에서 연간 대기로부터 흡수하는 순 유기탄소는 0.44 ton C $ha^{-1}yr^{-1}$로서, 이를 원화로 환산하면 약 44,000원 $ha^{-1}$의 가치를 갖는 것으로 추정되었다.

• 한국환경생태학회지
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• 제27권5호
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• pp.561-570
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• 2013

월악산국립공원 송계계곡에 발달되어 있는 소나무림에서 2011년 5월부터 2012년 4월까지 지상부와 지하부 생물량, 낙엽생산량, 낙엽층의 낙엽량, 그리고 토양의 유기탄소 분포를 조사하였으며, 탄소수지를 파악하기 위해 토양호흡량을 측정하였다. 지상부와 지하부 생물량에 분포되어 있는 유기탄소량은 각각 52.25, 14.52 ton C $ha^{-1}$ 이었으며, 낙엽층과 토양의 유기탄소량은 각각 4.71 ton C $ha^{-1}$, 58.56 ton C $ha^{-1}$ 50cm-$depth^{-1}$ 로 조사되었다. 조사지 소나무림의 전체 유기탄소량은 130.04 ton C $ha^{-1}$ 이었으며, 이중 51.4%가 식물체에 분포하는 것으로 나타났다. 본 소나무림에서 연간 광합성을 통하여 식물체에 고정된 유기탄소량은 4.26 ton C $ha^{-1}yr^{-1}$이었고, 층위별로는 교목층, 관목층, 초본층에 각각 4.12, 0.10 및 0.04 ton C $ha^{-1}yr^{-1}$의 유기탄소가 고정되었다. 조사기간 동안 낙엽생산을 통해 임상으로 유입되는 유기탄소량은 1.62 ton C $ha^{-1}$ 이었으며, 토양호흡을 통하여 방출되는 탄소량은 6.25 ton C $ha^{-1}yr^{-1}$으로 이중 미생물호흡과 뿌리호흡을 통해 방출되는 탄소량은 각각 3.19, 3.06 ton C $ha^{-1}yr^{-1}$이었다. 유기탄소 순 생산량과 미생물호흡량의 차이로 추정했을 때 본 소나무림에서 연간 대기로부터 순 흡수하는 유기탄소는 1.07 ton C $ha^{-1}yr^{-1}$ 로 조사되었다.

• 한국해양바이오학회지
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• 제15권2호
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• pp.82-89
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• 2023

Microalgae, as photosynthetic organisms, possess the ability to produce a diverse array of bioactive compounds. This study focused on the transformant Chlamydomonas reinhardtii dZL and subjected it to cultivation under varying light intensities (60, 120, 180, and 240 µmol/m²/s). Our aim was to assess the impact of light intensity on both microalgal biomass and carotenoid production. The cultivation took place in 80 mL bubble column photobioreactors, specifically the Multi-cultivator. Notably, the culture exposed to 240 µmol/m²/s exhibited the most rapid cell growth, surpassing even the cell concentration achieved at 180 µmol/m²/s by day 8. A detailed analysis of the specific irradiance rate over time unequivocally revealed a sharp decline in growth rates when the rate fell below 2 × 10^-10 µmol/cell/s. Although the culture with 60 µmol/m²/s yielded the highest carotenoid content (1.2% of dry weight), the culture exposed to 240 µmol/m²/s recorded the highest carotenoid concentration at 8.9 mg/L owing to its higher biomass. Our findings reveal the critical importance of maintaining a specific irradiance rate above 2 × 10^-10 µmol/cell/s to enhance biomass and carotenoid productivity. This study lays the groundwork for defining optimal light intensity conditions applicable to mass culture systems, with the objective of augmenting C. reinhardtii biomass and optimizing carotenoid productivity.

• Journal of Microbiology and Biotechnology
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• 제24권11호
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• pp.1559-1565
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• 2014

Cellulase and xylanase are main hydrolysis enzymes for the degradation of cellulosic and hemicellulosic biomass, respectively. In this study, our aim was to develop and test the efficacy of a rapid, high-throughput method to screen hydrolytic-enzyme-producing microbes. To accomplish this, we modified the 3,5-dinitrosalicylic acid (DNS) method for microwell plate-based screening. Targeted microbial samples were initially cultured on agar plates with both cellulose and xylan as substrates. Then, isolated colonies were subcultured in broth media containing yeast extract and either cellulose or xylan. The supernatants of the culture broth were tested with our modified DNS screening method in a 96-microwell plate, with a $200{\mu}l$ total reaction volume. In addition, the stability and reliability of glucose and xylose standards, which were used to determine the enzymatic activity, were studied at $100^{\circ}C$ for different time intervals in a dry oven. It was concluded that the minimum incubation time required for stable color development of the standard solution is 20 min. With this technique, we successfully screened 21 and 31 cellulase- and xylanase-producing strains, respectively, in a single experimental trial. Among the identified strains, 19 showed both cellulose and xylan hydrolyzing activities. These microbes can be applied to bioethanol production from cellulosic and hemicellulosic biomass.

• 한국식품과학회지
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• 제47권2호
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• pp.224-232
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• 2015

CP 처리의 CBPE 필름의 물리화학적 특성 및 생분해 특성에 대한 영향을 연구하였다. CP 처리는 CBPE 필름의 인장 강도, 신장률, 그리고 광투과도와 같은 물리적 특성에는 영향을 주지 않았으나, air-CP 처리는 CBPE 필름의 유연성, 수분 방벽 특성, 그리고 인쇄적성을 향상시켰다. Air-CP 처리는 저장 중 CBPE 필름 표면의 조도와 O와 N 원소를 포함한 작용기의 양을 증가시켰고, 인쇄적성, 열분해성, 그리고 미생물 분해성을 향상시켰다. 본 연구에서 CP 처리 후 CBPE 필름 표면에서 보여지는 노화 현상을 XPS 결과를 통해 확인할 수 있었다. 본 연구는 CP 처리가 CBPE 필름을 비롯하여 다른 생분해성 식품 포장재의 물리적 특성과 생분해성을 향상시킬 수 있는 기술로서 적용될 수 있다는 가능성을 보여주었다.

• 한국토양비료학회지
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• 제44권4호
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• pp.581-590
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• 2011

Organic farming is rapidly expanding worldwide. Crop growth in organic systems greatly depends on the functions performed by soil microbes, and nutrient supply weed suppression by green manure crops input. Four red-pepper production systems were compared: 1) bare ground (conventional system); 2) hairy vetch monoculture; 3) rye monoculture; and 4) hairy vetch-rye mixture. Soil inorganic N reached the peak at 30 DAI and hairy vetch monoculture was the highest ($192mg\;kg^{-1}$) and soil total carbon was fluctuated sporadically during the experiment. Carbohydrate and phenolic compounds in soil kept significantly higher in green manure crops systems from 10 DBI to 30 DAI, however the level was the maximum at 10 DBI (carbohydrate) and 30 DAI (phenolic comounds). Incorporation of green manure crops residue enhanced soil microbial biomass C and N throughout the growing season except that MBN in rye was reduced after incorporation. Green manure crops systems suppressed weed occurrence and, in particular, it was prominent in rye monoculture. Mineral elements composition and production in red-pepper fruits were markedly decreased in green manure crops systems although hairy vetch monoculture has come close to bare ground (NPK-applied). Therefore, it was suggested that higher biomass production should be performed not only to improve soil quality and suppress weeds but to yield suitable red-pepper fruits in green manure crops-based organic farming.

• 한국환경농학회지
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• 제28권4호
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• pp.397-402
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• 2009

In Korea, salt stress is one of the major problems limiting crop production and eco-environmental quality in greenhouse soil. The objective of this study was to evaluate the effectiveness of organic residues (Chinese milk vetch, maize stalk, rice straw, and rye straw) for reducing salt activity in greenhouse soil. Organic residues was incorporated with salt-accumulated soil (EC, 3.0 dS $m^{-1}$) at the rate of 5% (wt $wt^{-1}$) and the changes of electrical conductivity (EC) was determined weekly for 8 weeks under incubation condition at $30^{\circ}C$. The EC, microbial biomass carbon (MBC), and water soluble ions in soil was strongly affected by C/N ratio of organic residues. After 8 weeks incubation, the concentration of water soluble $NO_3{^-},\;Ca^{2+}$, and $Mg^{2+}$ was significantly decreased in organic residues having high C/N ratio (maize stalk, rice straw, and rye straw) incorporated soil compared to organic residues having lower C/N ratio (Chinese milk vetch) incorporated soil. The EC value in Chinese milk vetch incorporated soil was higher than control treatment. In contrast, maize stalk, rice straw, and rye straw amended soil was highly decreased the EC value compared to control and Chinese milk vetch applied soil after 4 weeks incubation. Our results indicated that incorporation of organic residues having high C/N ratio (>30) could reduce salt activity resulting from reducing concentration of water soluble ions.

• Journal of Microbiology and Biotechnology
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• 제32권10호
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• pp.1292-1298
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• 2022

Soil autotrophic bacterial communities play a significant role in the soil carbon (C) cycle in paddy fields, but little is known about how rhizosphere soil microorganisms respond to different long-term (35 years) fertilization practices under double rice cropping ecosystems in southern China. Here, we investigated the variation characteristics of rhizosphere soil RubisCO gene cbbL in the double rice ecosystems of in southern China where such fertilization practices are used. For this experiment we set up the following fertilizer regime: without any fertilizer input as a control (CK), inorganic fertilizer (MF), straw returning (RF), and organic and inorganic fertilizer (OM). We found that abundances of cbbL, 16S rRNA genes and RubisCO activity in rhizosphere soil with OM, RF and MF treatments were significantly higher than that of CK treatment. The abundances of cbbL and 16S rRNA genes in rhizosphere soil with OM treatment were 5.46 and 3.64 times higher than that of CK treatment, respectively. Rhizosphere soil RubisCO activity with OM and RF treatments increased by 50.56 and 45.22%, compared to CK treatment. Shannon and Chao1 indices for rhizosphere soil cbbL libraries with RF and OM treatments increased by 44.28, 28.56, 29.60, and 23.13% compared to CK treatment. Rhizosphere soil cbbL sequences with MF, RF and OM treatments mainly belonged to Variovorax paradoxus, uncultured proteobacterium, Ralstonia pickettii, Thermononospora curvata, and Azoarcus sp.KH33C. Meanwhile, cbbL-carrying bacterial composition was obviously influenced by soil bulk density, rhizosphere soil dissolved organic C, soil organic C, and microbial biomass C contents. Fertilizer practices were the principal factor influencing rhizosphere soil cbbL-carrying bacterial communities. These results showed that rhizosphere soil autotrophic bacterial communities were significantly changed under conditions of different long-term fertilization practices Therefore, increasing rhizosphere soil autotrophic bacteria community with crop residue and organic manure practices was found to be beneficial for management of double rice ecosystems in southern China.

• Journal of Microbiology and Biotechnology
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• 제22권3호
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• pp.371-377
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• 2012

The present study describes medium-chain-length polyhydroxyalkanoates (mcl-PHAs) production by the Pseudomonas Gl01 strain isolated from mixed microbial communities utilized for PHAs synthesis. A two-step fed-batch fermentation was conducted with glucose and waste rapeseed oil as the main carbon source for obtaining cell growth and mcl-PHAs accumulation, respectively. The results show that the Pseudomonas Gl01 strain is capable of growing and accumulating mcl-PHAs using a waste oily carbon source. The biomass value reached 3.0 g/l of CDW with 20% of PHAs content within 48 h of cultivation. The polymer was purified from lyophilized cells and analyzed by gas chromatography (GC). The results revealed that the monomeric composition of the obtained polyesters depended on the available substrate. When glucose was used in the growth phase, 3-hydroxyundecanoate and 3-hydroxydodecanoate were found in the polymer composition, whereas in the PHAs-accumulating stage, the Pseudomonas Gl01 strain synthesized mcl-PHAs consisting mainly of 3-hydroxyoctanoate and 3-hydroxydecanoate. The transcriptional analysis using reverse-transcription real-time PCR reaction revealed that the phaC1 gene could be transcribed simultaneously to the phaZ gene.