• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2003년도 학술발표대회 발표논문초록집
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• pp.75-75
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• 2003

Since the establishment of embryonic stem cell, pluripotency of the cells was known to allow differentiation of the cells into various cell types consisting whole body. Several protocols have been developed to induce expression of specific genes.. However, no precise protocol that will generate a single type of the cells from stem cells has been reported. In order to produce cells suitable for transplantion into brain of PD animal model, which arouse due to a progressive degeneration of dopaminergic neurons in midbrain, human embryonic stem cell (hESC, MB03) was transfected with cDNAs cording for tyrosine hydroxylase (TH). Successful transfection was confirmed by western immunoblotting. Newly transfected cell line (TH#2/MB03) was induced to differentiate by the two neurogenic factors retinoic acid (RA) and b-FGF. Exp. I) Upon differentiation using RA/ascorbic acid (AA), embryoid bodies (EB, for 4days) derived from hES cells were exposed to RA (10$^{-6}$ M)/AA (50 mM) for 4 days, and were allowed to differentiate in N2 medium for 7, 14, 21, or 28 days. Exp. II) When bFGF was used, neuronal precursor cells were selected for 8 days in N2 medium after EB formation. After selection, cells were expanded at the presence of bFGF (20 ng/ml) for another 6 days followed by a final differentiation in N2 medium for 7, 14, 21 or 28 days. By indirect immunocytochemical studies, proportion of cells expressing NF200 increased rapidly from 20% at 7 days to 70 % at 28 days in RA/AA-treated group, while those cells expressing NF160 decreased from 80% at 7 days to 10% at 28 days upon differentiation in N2 medium. However, in differentiation by RA/AA treatment system, there was a significant increase in proportion of neuron maturity (73%) at day 14 after N2 medium. TH#2/MB03 cells expressing TH are >90% when matured at the absence of either bDNF or TGF-$\alpha$. These results suggested that TH#2/MB03 cells could be differentiated in vitro into mature neurons by RA/AA.

• 한국통신학회논문지
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• 제30권5B호
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• pp.295-303
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• 2005

최근 들어, 인터넷 사용자의 폭발적인 증가로 인하여 차별화된 웹 서비스를 제공해주는 웹 응용프로그램들의 개발이 활발해지고 있다. 이에 따라 웹 서버내의 품질향상을 보장해주는 웹 QoS 기술은 전자상거래나 웹 호스팅 같은 부분에서 점점 더 중요한 문제로 대두되고 있다. 그러나 대부분의 웹 서버들은 FIFO 방식의 최선 서비스만을 제공하고 있으며, 정보의 중요도나 정보를 제공받는 사용자의 중요도에 따라 차별화된 품질보장을 제공하지 못한다. 본 논문에서는 클러스터링 웹 서버 환경에서 차별화 서비스를 위한 3단계 동적 부하분산 기법을 제안한다. 먼저, 커널 수준 접근 방식에서는 커널 상에 실시간 스케줄링 프로세스를 두어 웹 서버에서 수행중인 스케줄링 프로세스와 연동시키고, 커널 내부에서도 웹 서버에서 할당된 사용자 요청 우선순위를 유지하도록 한다. 둘째, 웹 서비스의 신뢰성과 반응속도를 개선하기 위하여 IP수준의 가장법과 터널링 기술을 이용하여 웹 서버의 부하를 분산을 수행한다. 셋째, 동적 부하분산을 제공하기 위해 SNMP중에 시스템 부하관련 MIB-II 정보를 검출하여 부하 분산에 반영한다.

• 한국정보처리학회논문지
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• 제6권3호
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• pp.699-709
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• 1999

This paper is aimed at defining items of analysis using SNMP MIB-II for the purpose of managing the Internet based network running on TCP/IP protocol, and then utilize these items, in conjunction with various Web technology and JAVA to design and implement a Web based interface of a management system to analyze the performance and status of the Internet. Among the required items in order to accomplish this task are utilization, interface packet transmission rate, I/O traffic ratio, and so on. Based on these items, the actual analysis is carried out by the Web interface according to the type of analysis. For instance, the interface executes the function of real-time analysis, collection processing, elementary analysis and detailed analysis. The demand of the user is fed into the Web interface which carried out a real-time analysis with the client system which in turn will eventually produce the results of the analysis. In order words, the interface acts as a mediator server for the analysis system. Furthermore, a protocol for exchange of data and messages between the server and the analysis system, the MATP protocol, was also designed. Finally, the results obtained through the system presented in this paper were displayed on screen according to the type of analysis. The system realized in this paper uses We technology and is independent of platform and allows the user to determine the performance of Internet at his/her own host according to the selected items of analysis.

• Journal of Chest Surgery
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• 제31권9호
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• pp.845-854
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• 1998

Background: S-2-(3 aminoprophlamino) ethylphosphorothioic acid(WR-2721) is one of the radical scavenging thiols. We tested its protective effects in the reperfused heart. Material and Method: The experimental setup was the constant pressure Langendorffs perfusion system. We investigated the radical scavenging properties of this compound in isolated rat hearts which were exposed to 20 minutes ischemia and 20 minutes reperfusion. Four experimental groups were used:group I, control, Amifostine 50 mg(1 mL) peritoneal injection 30 minutes before ischemia(group II), Amifostine 10 mg(0.2 mL) injection during ischemia through coronary artery(group III),and Amifostine 50 mg(1 mL) peritoneal injection 2 hrs before ischemia(group IV). The experimental parameters were the levels of latate, CK-MB, and adenosine deaminase(ADA) in frozen myocardium, the quantity of coronary flow,and left ventricular developed pressure, and it's dp/dt. Statistical analysis was performed using repeated measured analysis of variance and student t-test. Result: The coronary flow of group II and IV were less than group I and III at equilibrium state but recovery of coronary flow at reperfusion state of group II, III, and IV were more increased compared with group I. The change of systolic left ventricular devoloping pressure of group II and IV were less than control group at equilibrium state, which seemed to be the influence of the pharmacological hypotensive effect of amifostine. But it was higher compared with group I at reperfusion state. The lactic acid contents of group II were less than control group in frozen myocardium.(Group I was 0.20 0.29 mM/g vs Group II, which was 0.10 0.11 mM/g). The quantity of CK-MB in myocardial tissue was highest in group IV (P=0.026 I: 120.0 97.8 U/L vs IV: 242.2 79.15 U/L). The adenosine deaminase contents in the coronary flow and frozen myocardium were not significantly different among each group. Conclusion: Amifostine seemed to have significant cardioprotective effect during ischemia and reperfusion injuries of myocardium.

• 정보처리학회논문지C
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• 제8C권6호
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• pp.865-874
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• 2001

본 논문에서는 Home Phoneline Neworking Aliance(Home PNA) 장치에 연결된 많은 가입자들로부터 야기될수 있는 대역폭 서비스 불균형 현상을 해결하고 이 장비를 이용하는 가입자들을 관리하기 위한 웹 기반 Home PNA 장치 관리시스템을 구현하였다. Simple-Net-work Managment Protocol(SNMP) 를 탑대한 Home PNA장치를 관리하기 위해서 관리항목을 Muti Dewling Unit(MDU) 장비으 Management Information Base(MB)로 부터 추출한 MIB 오브젝트를 이용하여 시스템관리, 포트관리, 성능관리, 장애관리로 분류하였다. 시스템관리는 개별 MDU 장비에 대해 구성정보를 제공하고, 포트관리는 현재 가입되어 있는 가입자에 대한 현황정보를 제공하고 비인가 가입자에 대한 필터링을 수행한다 그리고 성능관리는 트렁크 라인과 가입자 라인의 트랙픽 정보를 제공한다. 마지막으로 장애관리는 예외 상황에 대한 장애로그 및 trap 메시지를 제공한다. 구현된 시스템의 운영성을 시험하기 위하여 실제 네트워크에서 그 동작성을 검증하였다.

• Animal Bioscience
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• 제36권8호
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• pp.1252-1262
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• 2023

Objective: Microencapsulation technologies have been developed and successfully applied to protect the probiotic bacterial cells damaged by environmental exposure. This study aimed to investigate the effects of microencapsulation of Lactobacillus plantarum MB001 on the growth performance, ileal nutrient digestibility, jejunal histomorphology and cecal microbiome of broiler chickens in a tropical climate. Methods: A total of 288 one-day-old female broilers (Ross 308) were randomly allocated into 4 groups (6 replicates of 12 birds). Treatments included, i) a basal diet (NC), ii) NC + avilamycin (10 mg/kg) (PC), iii) NC + non-encapsulated L. plantarum MB001 (1×10⁸ colony-forming unit [CFU]/kg of diet) (N-LP), iv) NC + microencapsulated L. plantarum MB001 (1×10⁸ CFU/kg of diet) (ME-LP). Results: Dietary supplementation of ME-LP improved average daily gain, and feed conversion ratio of broilers throughout the 42-d trial period (p<0.05), whereas ME-LP did not affect average daily feed intake compared with NC group. Both N-LP and ME-LP improved apparent ileal digestibility of crude protein and ether extract compared with NC group (p<0.05). The broilers fed ME-LP supplemented diet exhibited a beneficial effect on jejunal histomorphology of villus height (VH), crypt depth (CD) and villus height to crypt depth ratio (VH:CD) of broilers compared to NC group (p<0.05). At the phylum level, Firmicutes was enriched (p<0.05) and Proteobacteria was decreased (p<0.05) only in the ME-LP group. At the genus level, the ME-LP diets increased (p<0.05) the number of both Lactobacillus and Enterococcus compared to NC, PC, and N-LP groups (p<0.05). Conclusion: Microencapsulation assists the efficient functioning of probiotics. ME-LP could be potentially used as a feed additive for improvement of cecal microbiota, gut integrity and nutrient utilization, leading to better performance of broilers.

• 운동영양학회지
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• 제17권2호
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• pp.25-34
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• 2013

This study was conducted to investigate the relationship between ACE gene polymorphism and muscle damage parameters after eccentric exercise. 80 collegiate males were instructed to take an eccentric exercise with the elbow flexor muscle through the modified preacher curl machine for 2 sets of 25 cycles (total 50 cycles). The maximal isometric strength, muscle soreness, creatine kinase (CK), and myoglobin (Mb) were measured before exercise, and 0, 24, 48, 72, and 96 hrs after exercise. The result showed that after the eccentric exercise, the maximal isometric strength significantly decreased by more than 50% (p < 0.001) and the muscle soreness, CK, and Mb significantly increased compared to those before the exercise (p < 0.001). The ACE gene polymorphism of the subjects was classified using real-time polymerase chain reaction (real-time PCR). The result showed that it consisted of 38 cases of type II (46.4%), 33 cases of type ID (43.4%), and 9 cases of type DD (10.2%). The Hardy-Weinberg equilibrium for ACE gene polymorphism was shown to have p = 0.653, which showed that each allele was evenly distributed. Although significant differences in the changes in the maximal isometric strength, muscle soreness, CK, and Mb were found according to time course (p < 0.001), no significant differences in the changes in the maximal isometric strength, muscle soreness, CK, and Mb were found according to ACE gene polymorphism. Furthermore, no significant difference in the changes in the muscle damage parameters was found according to interaction between ACE gene polymorphism and time course (p > 0.05). In conclusion, the level of the muscle damage parameters changed in the injured muscle after eccentric exercise, but these changes in the muscle damage parameters were not affected by ACE gene polymorphism. The result of this study indicates that ACE gene is not a candidate gene that explains muscle damage.

• Bulletin of the Korean Chemical Society
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• 제23권12호
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• pp.1769-1772
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• 2002

Previous $^{19}F\;and\;^{1,2}H$ electron-nuclear double resonance (ENDOR) study of fluorometmyoglobin (MbF) in frozen-solution state provided sensitive tools sensing subtle structural changes of the heme that are not obtainable from X-ray. [Fann et al., J. Am. Chem. Soc. 1995, 117, 6019] Because of the intrinsic inhomogeneouse EPR line broadening effect of MbF in frozen-solution state, detection of the intrinsic inhomogeneouse EPR line broadening effect of MbF in frozen-solution state, detection of the electronic and geometrical changes of the heme ring itself and the proximal histidine by using $^{14}N$ CW ENDOR was interfered. In the present study, hyperfine-sensitive $^{14}N$ Mims ENDOR technique of pulsed-EPR was employed to probe the changes. With two different $\tau$ values of 128 and 196 ns, $^{14}N$ ENDOR signals of the heme and proximal histidine were completely resolved at $g'_{II}(=g_e=2)$. This study present that X-band $^{14}N$ Mims ENDOR sequence can sensitively detect the small changes of the spin densities and p orbital populations of the proximal and the heme nitrogens, caused by ligand and pH variation of the distal site.

• 농업과학연구
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• 제26권2호
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• pp.19-24
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• 1999

본 실험은 배지 및 호르몬이 돼지 난포란의 체외성숙 및 체외발생능에 미치는 영향을 구명하기 위하여 실시하였다. 기본 배지는 TCM-199, Waymouth MB751/1 및 BMOC-II를 사용하였으며, 각각의 배지에 첨가된 호르몬은 hCG와 FSH를 사용하였다. 실험에서 얻어진 결과를 요약하면 다음과 같다. 1. TCM-199을 기본배양액으로 하여 돼지난포란을 체외배양하였을 때, 체외성숙률은 배양액내에 hCG 단독첨가의 경우가 78.05%, FSH 단독첨가에서 72.50%, hCG와 FSH의 병용첨가의 경우가 67.50%로서 호르몬을 첨가하지 않은 처리구의 54.29%에 비하여 유의적(P<0.05)으로 높은 성적을 나타냈으며, 수정후 같은 배지조건하에서 난할률은 호르몬 첨가의 경우(hCG 46.88%, FSH 31.04%, hCG+FSH 37.04%)가 무첨가구(89.47%)에 비하여 유의적(P<0.05)으로 낮았다. 2. Waymouth MB751/1을 배양액으로 하여 체외배양하였을 때, 체외성숙률은 배양액내에 hCG가 71.43%, hCG+FSH 병용첨가의 경우가 80.00%로서 호르몬을 첨가하지 않은 처리구의 25.64%에 비하여 유의적(P<0.05)으로 높은 성적을 나타냈다. 그러나, 난할률은 호르몬 첨가의 경우(hCG 46.67%, FSH 36.00%, hCG+FSH 35.71%)가 무첨가구(60.00%)에 비하여 낮은 성적을 나타냈다. 3. BMOC-II를 기본배양액으로 하여 체외배양 하였을 때, 체외성숙률은 배양액내에 hCG 단독첨가 66.7%, FSH 단독첨가 91.89%, hCG+FSH 병용첨가 81.82% 및 무첨가구 66.67%로 각각 나타나서 FSH 단독첨가의 경우가 가장 높은 성적을 나타냈다. 또한, 난할률은 hCG 단독첨가 79.17%, FSH 단독첨가 50.00%, hCG_FSH 병용첨가 66.67% 및 무첨가 81.82%로 각각 나타났다.

• 대한임베디드공학회논문지
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• 제1권1호
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• pp.20-23
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• 2006

본 논문은 MB-OFDM UWB(Multi Band-Orthogonal Frequency Division Multiplexing Ultra Wide Band) 시스템을 위한 변형된 가변 파라미터를 이용한 큐빅 인터폴레이터를 제안한다. MB-OFDM UWB 시스템은 고속의 동작속도가 필요하기 때문에, 기존 가변 파라미터를 이용한 큐빅 인터폴레이터에 병렬 처리 기술과 파이프라인 기법을 동시에 적용한다. 실험 결과, Stratix II 2S60F101020C3 디바이스를 타켓으로 최대지연경로 속도와 최대지연경로 주기가 각각 최대 88.79MHz와 11.262ns가 되었고, 동작속도는 최대 대략 200% 이상 향상되었음을 알 수 있다.