• Journal of Embryo Transfer
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• v.31 no.2
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• pp.123-129
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• 2016

This study is performed to evaluate the effect of insulin in the porcine parthenogenetic embryo development. In porcine embryo culture, insulin is helpful factor in the process of embryo development. To identify this, insulin is used in pig embryos development. Therefore, this study was performed to investigate the effect of insulin on early embryonic development in pigs. For that, insulin positive or negative (0, 10 ug/mL) was supplemented in the porcine IVM media and then compared two groups divided by the cytoplasm of the black groups and white ring groups based on the distribution of lipid material of the cell cytoplasm in microscope. In maturation rates of porcine oocytes, significant higher black group rates were shown in the insulin positive groups compared with other groups ($56.0{\pm}2.1$ vs $46.2{\pm}0.3$). In the embryo culture, black groups were showed the significant higher cleavage rates ($82.1{\pm}0.8$, $78.3{\pm}0.1$ vs $63.2{\pm}0.3$, $63.4{\pm}0.0$), and blastocyst formation rates ($15.5{\pm}3.6$, $16.6{\pm}0.4$ vs $11.7{\pm}1.3$, $7.4{\pm}0.2$) regardless of whether the addition of insulin. Also, black groups were showed higher cell number of blastocyst ($33.2{\pm}2.5$, $35.5{\pm}2.6$ vs $31.2{\pm}2.1$, $31.3{\pm}2.2$). In conclusion, supplement of insulin producing black group in vitro maturation, it was effective in vitro maturation and embryonic development of pig embryos.

• Journal of Embryo Transfer
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• v.31 no.3
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• pp.235-242
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• 2016

This study tested the association between genotypes of the single nucleotide polymorphism (SNP) marker, rs81437607 and capric acid (FA_C10_0) compositions in longissimus dorsi muscle in pigs. Eighteen fatty acid (FA) compositions were measured in a total of 974 $F_2$ animals among 1,106 $F_2$ progeny produced between Landrace and Jeju Black Pig (JBP). Among FA compositions tested, we identified a cluster of highly significant SNPs for capric acid compositions on 58 Mb position of Sus scrofa chromosome 12 (SSC12) using genome-wide association study (GWAS) with $F_2$ genotypes from SNP panel analysis. GWAS results showed that the rs81437607 was the highest trait-related SNP marker with capric acid levels. Three genotypes (C/C, C/T and T/T) of rs81437607 marker were found in $F_2$ population by further pyrosequencing. Association analysis results showed the significant differences between rs81437607 genotypes and capric acid compositions (P<0.05). The $F_2$ pigs harboring rs81437607 C/C ($0.119{\pm}0.002%$) and C/T ($0.116{\pm}0.002%$) genotypes showed additively higher levels of capric acid content than those of T/T homozygotes ($0.109{\pm}0.002%$) ($P=1.30{\times}10^{-12}$). These results suggested that the genetic variations of rs81437607 may be helpful to find causative variants and assist as molecular genetic markers for improving the capric acid contents in longissimus dorsi muscle in pigs.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.101-101
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• 2003

Main strategy for a treatment of Parkinson's disease (PD), due to a progressive degeneration of dopaminergic neurons, is a pharmaceutical supplement of dopamine derivatives or ceil replacement therapy. Both of these protocols have pros and cons; former exhibiting a dramatic relief but causing a severe side effects on long-term prescription and latter also having a proven effectiveness but having availability and ethical problems Embryonic stem (ES) cells have several characteristics suitable for this purpose. To investigate a possibility of using ES cells as a carrier of therapeutic gene(s), human ES (hES, MB03) cells were transfected with cDNAs coding for tyrosine hydroxylase (TH) in pcDNA3.1 (+) and the transfectants were selected using neomycin (250 $\mu /ml$). Expression of TH being confirmed, two of the positive clone (MBTH2 & 8) were second transfected with GTP cyclohydrolase 1 (GTPCH 1) in pcDNA3.1 (+)-hyg followed by selection with hygromycin-B (150 $\mu /ml$) and RT-PCR confirmation. By immune-cytochemistry, these genetically modified but undifferentiated dual drug-resistant cells were found to express few of the neuronal markers, such as NF200, $\beta$-tubulin, and MAP2 as well as astroglial marker GFAP. This results suggest that over-production of BH4 by ectopically expressed GTPCH I may be involved in the induction of those markers. Transplantation of the cells into striatum of 6-OHDA- denervated PD animal model relieved symptomatic rotational behaviors of the animals. Immunohistochemical analyses showed the presence of human cells within the striatum of the recipients. These results suggest a possibility of using hES cells as a carrier of therapeutic gene(s).

• Journal of Oral Medicine and Pain
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• v.23 no.1
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• pp.11-20
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• 1998

저자는 치과용 레이저를 이용한 상아질지각과민증 치료효과를 임상적으로 평가하고자 상아질지각과민증환자 24명을 대상으로 각 환자마다 상아질 지각과민 치아 중 레이저로 치료 할 치아1개(실험군)와 치료하지 않을 치아 1개(대조군)를 선정하여, 치과용 air-syringe에 의한 냉자극과 치과용 탐침자에 의한 기계적 자극을 가한 후 Visual Analogue Scale(VAS)을 통해 지각과민증 정도를 평가하였으며, 이후 실험군 치아의 치경부 마모면에 광섬유 레이저에너지 전달방식의 펄스형 Nd:YAG 레이저의 일종인 Sunlase Master(Sunrise Technologies Inc., U.S.A., 파장 1064nm, 펄스지속시간 120(sec, 광섬유직경 320(m)를 사용하여, 0.3-1.0W, 10Hz 조건으로 환자의 반응에 따라 1회2분을 초과하지 않는 범위 내에서 비접촉식과 접촉식을 병행하여 3회에 걸쳐(초진 당일, 1일후, 3일후) 레이저를 조사한 후 처음조사 직후, 그리고 초진 1일후, 3일후, 7일후, 14일후 VAS를 채득하여 조사전과 비교, 분석, 평가하였던 바, 상아질 지각과민증이 각각 약 50%, 약36%, 약45%, 약 53% 감소하였으나 (p(0.05) 경시적으로는 유의한 차이를 보이지 않았다. 그리고 24명중 9명에 대해서는 초진 30일 후 까지 레이저조사치료효과를 평가하였던 바, 상아질지각과민증이 처음조사 직후, 감소하였으며 (p(0.05)역시 경시적으로는 유의한 차이를 보이지 않았다. 초진 30일 후 상아질지각과민증이 초진 14일 후에 비해 약간 증가되는 경향을 보여, 레이저조사 치료효과가 약화되는 경향인지 여부에 대해 향후 추가적인 연구가 필요할 것으로 사료된다.안되었다.기란 현실적으로 불가능하므로, 본 연구에서는 이러한 제약점을 극복할 수 있는 근사적인 지체시간을 계산하는 방법을 제시한 점에서 의미를 갖을 수 있다.수들은 직업의 선택이나 소득을 예측하기 위한 요소들로 포함될 수 없었다. 따라서 후속연구에서는 이를 보완해야 할 것이며, 최근 들어 우리 나라에서도 재택근무에 대한 관심이 대두되고 있으나 아직 개념정의나 그 중요성과 가치, 그리고 실태 파악과 같은 연구가 활발히 이루어지지 못하고 있으므로 이에 대한 심층적인 연구가 행해져야 할 것이다.d similar flower proceeding dates in all branches. but "Daepung" showed similar flower proceeding dates in all branches.est in HB. Mean period of wetting duration was in the order of DS＞HB＞MB, while the dew point depression was greatest in DS.ANCOVA, Pearson correlation을 이용하여 분석하였으며, 그 결과는 다음과 같다. 캠프 프로그램은 소아 당뇨병 환자의 자기 효능을 증진시키고 환자 역할 행위 이행을 높여주는데 효과적 이었다. 소아 당뇨병 환자의 자기 효능은 환자 역할 행위 이행과 순 상관 관계가 있어, 자기 효능이 증진될수록 환자 역할 행위 이행 정도가 높아졌다. 무조건 사주지 않는다(8.0%), 무조건 사준다(3.1%)로 식품광고에 나오는 식품 요구시 부모의 70.3%가 거절하는 것으로 나타났다. 거절 이유는 건강에 나쁘다는 것이 가장 큰 이유였으며 강남과 강북 어린이간에 유의적인 차이가 있었다(p＜0.001).

• Journal of The Korean Astronomical Society
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• v.28 no.1
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• pp.31-43
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• 1995

We present UBVRI CCD photometry of the Type Ie supernova SN 19941 in M51 which was discovered on April 2, 1994 (UT). UBVRI CCD photometry of SN 1994 I were obtained for the period of the first two months from April 4, 1994, using the Seoul National University Observatory 60 cm telescope. The light curves of SN 19941 show several interesting features: (a) SN 19941 reaches the maximum brightness at B-band on April 8.23 (B = 13.68 mag), at V-band on April 9.10 (V = 12.89 mag), and at I-band on April 10.32 (I = 12.48 mag); (b) The light curves around the maximum brightness are much narrower than those of other types of supernovae; (c) The light curves after the peak decline more steeply than those of other types of supernovae; and (d) The colors get redder from $(V-R){\approx}0.2 mag ((V - I){\approx} 0.3 mag, (B - V){\approx}0.7 mag)$ on April 4 to $(V-R){\approx}0.6 mag ((V-1){\approx}0.9 mag, (B-V){\approx}1.3 mag)$ on April 18. Afterwards (V - R) colors get bluer slightly $(by\~0.005 mag/day)$, while (V-I) colors stay almost constant around $(V-1){\approx}1.0 mag$. The color at the maximum brightness is (B-V)=0.9 mag, which is $\~1$mag redder than the mean color of typical Type la supernovae at the maximum brightness. The light curves of SN 1994I are similar to those of the Type Ie supernova SN 1962L in NGC 1073. Adopting the distance modulus of $(m-M)_0 = 29.2 mag$ and the reddening of E(B - V) = 0.45 mag [Iwamoto et al. 1994, preprint for ApJ], we derive absolute magnitudes at the maximum brightness of SN 1994I, Mv(max) = -17.7 mag and MB(max) = -17.4 mag. This result shows that SN 1994I was $\~2$mag fainter at the maximum brightness compared with typical Type Ia supernovae. A narrower peak and faster decline after the maximum in the light curve of SN 1994I compared with other types of supernovae indicate that the progenitor of SN 1994I might be a lower mass star compared with those of other types of supernovae.

• Applied Chemistry for Engineering
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• v.20 no.4
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• pp.402-406
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• 2009

$TiO_2$ thin films were surface-modified with atmospheric plasma and their photocatalytic activities were evaluated. The films were deposited on glass plates by dip-coating in a $TiO_2$ sol-gel solution and sintered at various temperatures for various times. Nitrogen plasma was used for the modification and the experiments were carried out varying operational parameters such as discharge power and treatment time. Photocatalytic activity was evaluated based on the degradation efficiency of methylene blue (MB) under irradiation of UV-A and fluorescent light. According to XPS analysis, a little amount of nitrogen was found to be doped in the film surface after the modification. As a result, photocatalytic activity increased under irradiation of UV-A and fluorescent light, especially fluorescent light.

• Biomolecules & Therapeutics
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• v.24 no.4
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• pp.402-409
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• 2016

It has been found that 4-isopropyl-2,6-bis(1-phenylethyl)phenol (KTH-13), a novel compound isolated from Cordyceps bassiana, is able to suppress tumor cell proliferation by inducing apoptosis. To mass-produce this compound, we established a total synthesis method. Using those conditions, we further synthesized various analogs with structural similarity to KTH-13. In this study, we aimed to test their anti-cancer activity by measuring anti-proliferative and pro-apoptotic activities. Of 8 compounds tested, 4-methyl-2,6-bis(1-phenylethyl)phenol (KTH-13-Me) exhibited the strongest anti-proliferative activity toward MDA-MB 231 cells. KTH-13-Me also similarly suppressed the survival of various cancer cell lines, including C6 glioma, HCT-15, and LoVo cells. Treatment of KTH-13-Me induced several apoptotic signs in C6 glioma cells, such as morphological changes, induction of apoptotic bodies, and nuclear fragmentation and chromatin condensation. Concordantly, early-apoptotic cells were also identified by staining with FITC-Annexin V/PI. Moreover, KTH-13-Me highly enhanced the activation of caspase-3 and caspase-9, and decreased the protein level of Bcl-2. In addition, the phosphorylation levels of Src and STAT3 were diminished in KTH-13-Me-treated C6 cells. Therefore, these results suggest that KTH-13-Me can be developed as a novel anti-cancer drug capable of blocking proliferation, inducing apoptosis, and blocking cell survival signaling in cancer cells.

• Korean Journal of Microbiology
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• v.30 no.2
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• pp.101-107
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• 1992

Cytochrome c oxida5e from chemotrophically grown R p , geliitinosu was purified by cytochrome c affinity chromatography and DEAE-Sephacel ion exchange chromatography. The molecular weight of the cytochrome c oxidase was approximately 110.000 Da by sephacryl s-300 gel chromatography and approximately 52, 000 Da by SDS-gel electrophoresis, respectively. Therefore. cytochrolne c oxidase of Rps. gehtinosu seems to be dimer. The cytochrome c oxidasc was very sensitive to temperature. It's Km and Vmax were 20 pM and 44 unitlmg protein for horsc heart cytochrome c as a substrate. respectively, and its optimum pH and temperature were 6.4 and 25$^{\circ}$C. respectively. The absorption peaks of the reduced cytochrome c oxidase showed at 554 nm, 523 nm. and 422 nm. The activiiy of cytochrome c oxidase was inhibited by KCN, and NaN3, but not by CO, antimycir~ A. and myxothiazol. The cytochrome c-551 was produced either in phototrophically or chemotrophically grown Rps. gelaiinosci. The rcduced cytochrome c-551 was oxidized by b-type cytochrome c oxidase from Rp.v. gc.lrtino.sc~. Km and Vmax of cytochrome c oxidase was 26 pM and 31 unitlnlg protein For cytochrome c-551 as a substrate. respectively. Thercfore. thc electron transfer chain of chemotrophically grown Rps. glatinosa seems lo be ubiquinol cytochrome bc, complex -'cytochrome c-55lMb-type cytochrome c oxidase+02.

• The Journal of Korean Physical Therapy
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• v.18 no.3
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• pp.71-78
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• 2006

Purpose: The purpose of this study was to investigate how induced microcurrent shoes influenced changes of the blood circulation in patients with Plantar Fasciitis. Methods: Initially, the subjects were comprised of 5 males and 5 females, who agreed with this research and are more than fifty years old, but 4 of those were dropped during the experiment. They all have plantar fasciitis and pain on their feet. Subjects wore the induced microcurrent shoes for more than 4 hours everyday during 4 weeks. When they wore those shoes, they also wore the specially produced shocks made of silver-mixed thread and they were asked to avoid intense exercise. Assessments were carried out before and after walking on a treadmill and we measured changes between the test before and after 4 weeks. In the examination of the before test, general shoes were used, and in the examination of the after test, induced microcurrent shoes were used. Temperature difference was measured by thermograpy DOREX spectrum 9000MB(USA). Subjects walked total 20 minutes and during gait, the walking rate on a treadmill was increased from 2Km/h to 3Km/h after 10 minutes. We measured plantar temperature by thermograpy especially both heel, 1st, and 5th metatarsal areas. Results: Firstly, in comparison of the blood circulation on the left and right foot at 0 week and 4 weeks, it tended toward increasing blood circulation but there was. no statistically significant difference (p>0.05). Secondly, in comparison of the blood circulation before and after treadmill with the induced microcurrent shoes, the blood circulation of the heel and the 5th area on the right foot was increased to all subjects(p<0.05). Conclusion: The results of this study revealed that subjects showed tendency to increase blood circulation in both right and left feet after wearing microcurrent shoes and specially after walking treadmill at 4 weeks. Therefore induced microcurrent shoes are useful to improve blood circulation for patients with plantar fasciitis.

• Journal of Chest Surgery
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• v.21 no.3
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• pp.425-446
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• 1988

After 24 hours of preservation under 15 mmHg perfusion pressure the recovery rates of isolated canine hearts were determined. Preservation was performed in a cold room maintained at 4*C with 4 different types of perfusates bubbled with a mixture of 95% 0y and 5% CO~ using a modified perfusion unit designed in our institute. The perfusates used were as follows; Group 1: Krebs-Henseleit solution, Group 2: Krebs solution added by albumin and PGE1. Group 3: Modified Wicomb*s solution, Group 4: Modified Collin*s solution. The extent of myocardial recovery was evaluated using a modified isolated carmine perfusion model by measuring heart rate, systolic arterial pressure, left atrial pressure[LAP] and cardiac output. In addition to the above hemodynamic parameters, biochemical and enzymatic assays from perfusates and electron microscopic changes of the myocardium were also studied. The results were as follows; 1] The heart recovery rates were 41.6%, 53.4% and 108.9% in groups 1, 2 and 3, respectively, and group 3 elicited the best result[p< 0.001]. The heart beat was never recovered in group 4. 2] Recovered systolic arterial pressures[mmHg] were 63.3% in group 1, 94.9% in group 2 and 94.3% in group 3. 3] LAPs[mmHg] were 20 in group 1, 13.5 in group 2 and 11.2 in group 3, which suggested that the best myocardial preservation was elicited in group 3[p< 0.05]. 4] Cardiac output, the sum of aortic stroke volume and coronary leakage, were 69.1% in group 2, and 90.7% in group 3, but these were not statistically significant[p=0.24]. No aortic stroke output was measured in group 1 and 4. 5] The degree of myocardial edema increase was 17.5` in group 1, 24.6% in group 2, 20.9% in group 3 and 55.3% in group 4. But there were no statistical differences in each group[p= 0.08]. 6] CPK-MB[U/L] levels were increased 750% and 332%[p< 0.05], glucose levels[mg/dl] 60.5% and 78.2% and SGOT[U/L] levels 523% and 333%, in groups 2 and 3, respectively. Biochemical and enzymatic assays could not be performed in group 1 and group 4, because of poor recovery of heart beat. 7] Electron microscopic findings in the myocardium of most groups revealed slight to moderate muscle cell and mitochondrial edema. But all these findings were within the limits of reversible change. From these above results, it is suggested that modified Wicomb*s solution seems to be the most useful physiologic salt solution for preservation of the heart. We propose that after further study and improvement, our portable continuous hypothermic perfusion system will contribute to the development of a better preservation method for donor hearts for human heart transplantation.