• Journal of Food Hygiene and Safety
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• v.19 no.3
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• pp.161-166
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• 2004

We investigated the antiplatelet effect of a newly synthesized guanidine derivative KR-32558, a sodium-hydrogen exchanger-1 (NHE-1) inhibitor, together with the elucidation of the possible mechanisms of action. KR-32558 concentration -dependently inhibited the aggregation of washed rabbit platelets induced by collagen (10 ${\mu}g/ml$) with an $IC_{50}$ value of 85.9 ${\mu}M$, but with much weaker potency against aggregation induced by thapsigargin (0.5 ${\mu}M$) or A23187 (5 ${\mu}M$). And had no effect on platelet aggregation induced by arachidonic acid (100 ${\mu}M$), thrombin (0.05 U/ml) and U46619 (1 ${\mu}M$) up to 100 ${\mu}M$. KR-32558 completely inhibited the $[Ca^{2+}]_i$ mobilization induced by collagen at concentration of 100${\mu}iM$. Taken together, these observation suggest that KR-32558 selectively inhibited collagen-mediated platelet aggregation by blocking the cytoplasmic calcium mobilization in addition to NHE-1 inhibition.

• Journal of Environmental Science International
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• v.14 no.2
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• pp.141-156
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• 2005

The importance of atmospheric conditions for the assessment of an air pollution situation has been demonstrated by their influence on the various compartments of an air pollution system, comprising all stages from emission to effects. Especially, air pollutants dispersion phenomenon are very sensitive according to wind data. But the discussions of how to apply representative meteorological data in air pollution dispersion model are not frequent in Korean environmental assessment processes. In this study, we investigated the difference of air pollutants dispersion phenomenon using U.S EPA ISCLT3 model according to applying the different meteorological data observed at two points for Seongseo industrial complex of Daegu. Two points are the spot site of Seongseo industrial complex and Daegu meteorological observatory. The winds speed of the spot site were smaller than those of Daegu meteorological observatory. In the winter season, the differences came to about $64\%$ for the period$(I\;February\;2001\~31\;January\;2002)$. Wind directions were also fairly different at two points. The air pollutants dispersion phenomenon estimated from our numerical experiments were also fairly different owing to the meteorological conditions at two points.

• Clinical and Experimental Reproductive Medicine
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• v.31 no.4
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• pp.217-223
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• 2004

Objective: To evaluate the effects of recombinant FSH (rFSH) and urinary FSH (uFSH) on the gene expressions of human endometrial stromal cells in vitro. Methods: Endometrial tissue was obtained from a pre-menopausal women undergoing hysterectomy. Primary endometrial stromal cells were isolated and in vitro cultured with FBS-free DMEM/F-12 containing 0, 10, 100, and 1, 000 mIU/ml of rFSH and uFSH for 48 hours, respectively. Total RNA was extracted from the cultured cells and subjected to real time RT-PCR for the quantitative analysis of progesterone receptor (PR), estrogen receptor $\alpha/\beta$ (ER-$\alpha/\beta$), cyclooxygenase 2 (Cox-2), leukemia inhibitory factor (LIF), homeobox A10-1 and -2 (HoxA10-1/-2). Results: Both hormone treatments slightly increased (< 3 folds) the expressions of PR, ER-$\beta$ and HoxA10-1/-2 gene. However, ER-$\alpha$ expression was increased up to five folds by treatments of both FSH for 48 hours. The LIF expression by the 10 mIU/ml of uFSH for 12 hours was significantly higher than that of rFSH (p<0.01). After 24 hours treatment of two kinds of hormones, the expression patterns of LIF were similar. The 100 and 1, 000 mIU/ml of rFSH induced significantly higher amount of Cox-2 expression than those of uFSH, respectively (p<0.05). Conclusion: This study represents no adversely effect of exogeneous gonadotropins, rFSH and uFSH, on the expression of implantation related genes. We suggest that rFSH is applicable for the assisted reproductive technology without any concern on the endometrial receptivity.

• The Journal of the Korea Contents Association
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• v.12 no.2
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• pp.349-357
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• 2012

Although u-healthcare service is emerged as an alternative method for effective chronic disease management services, the service has not yet been applied for real healthcare setting. The objective of this study is to explore the doctors' perception and influential factors on intention to use u-healthcare service. We conducted survey for physicians about u-healthcare service provision to compare characteristics by different groups. In addition, logistic regression analysis is conducted to find out factors affecting the usage intention. As a result, doctors responded only 16.0% of total respondents had experience of u-healthcare services, but also showed that as high as 70.1% had intention to use service. Also, respondents answered that u-healthcare services is appropriate to apply for chronic disease prevention and diabetes and hypertension are suggested as the most appropriate diseases in order. The intention to use the u-healthcare service by non-university hospital doctors was 3.7 times higher than university hospital doctor. This study shows that identifying the differences of doctors' awareness and also the intention to use about the u-healthcare services will contribute to develop more effective business model.

• The Korean Journal of Physiology and Pharmacology
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• v.16 no.4
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• pp.281-285
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• 2012

A previous animal study has shown the effects of erythropoietin (EPO) and its non-erythropoietic carbamylated derivative (CEPO) on neurogenesis in the dentate gyrus. In the present study, we sought to investigate the effect of EPO on adult hippocampal neurogenesis, and to compare the ability of EPO and CEPO promoting dendrite elongation in cultured hippocampal neural progenitor cells. Two-month-old male BALB/c mice were given daily injections of EPO (5 U/g) for seven days and were sacrificed 12 hours after the final injection. Proliferation assays demonstrated that EPO treatment increased the density of bromodeoxyuridine (BrdU)-labeled cells in the subgranular zone (SGZ) compared to that in vehicle-treated controls. Functional differentiation studies using dissociated hippocampal cultures revealed that EPO treatment also increased the number of double-labeled BrdU/microtubulea-ssociated protein 2 (MAP2) neurons compared to those in vehicle-treated controls. Both EPO and CEPO treatment significantly increased the length of neurites and spine density in MAP2(+) cells. In summary, these results provide evidences that EPO and CEPO promote adult hippocampal neurogenesis and neuronal differentiation. These suggest that EPO and CEPO could be a good candidate for treating neuropsychiatric disorders such as depression and anxiety associated with neuronal atrophy and reduced hippocampal neurogenesis.

• Speech Sciences
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• v.10 no.3
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• pp.145-155
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• 2003

This study compared $F_{0}$ and the first three formants of eight Korean monophthongs produced by nine transgenders (male to female) to those of eighteen normal adults. Voice analysis was done by Praat (version 4.049). A one-way ANOVA with Tukey HSD post hoc tests were performed to determine statistical differences in $F_{0}$ and formant values obtained from transgenders, and normal male and female subjects. Results indicated that there was no significant difference in $F_{1}$ of /u/, /$\Lambda$/, and /o/, $F_{2}$ of /u/, /$\Lambda$/, and /i/ and $F_{3}$ of /u/ among the 3 groups (transgenders, normal males and normal females). However, in the comparison of transgenders vs. males, a significant difference was observed in $F_{0}$ of /o/, and $F_{2}$ of /i/, /a/, /e/, and /${\ae}$/ and $F_{3}$ of /e/. Furthermore, in the comparison of transgenders vs. females, a significant difference was also observed in $F_{0}$ of all vowels, $F_{1}$ of /i/, /$\alpha$/, /e/, /${\ae}$/, and /i/. $F_{2}$ of /i/, and /${\ae}$/, and $F_{3}$ of /i/, /$\alpha$/, /$\Lambda$/, /e/, /${\ae}$/, /i/, and /o/. Also, perceptual judgment of the transgenders' voice came out somewhat correlated strongly with their $F_{0}$ values but not much with the formant values. It was concluded that the transgenders' acoustic parameters are placed in between those of the normal males and females in. terms of fundamental and formant frequency analyses of vowels. Thus, it was assumed that those differences might stem from the transgenders' original big resonating cavities.

• Natural Product Sciences
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• v.13 no.2
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• pp.123-127
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• 2007

We investigated effect of small black soybean fraction (SBSF) T cell-mediated responses for tumor surveillance and proliferation in leukemia cells in vitro. Each SBSF butanol fraction (SBSFBu) and SBSF chloroform fraction (SBSFCh) was administered p.o. once a day far 21 days in BALB/c mice and then levels of serum cytokines and subpopulation of lymphocytes were measured. Moreover, SBSF fraction was treated into the cultured various cell lines for proliferation in leukemia cell lines, NO production by RAW264.7 cells, and expression of p53 gene in U937 leukemia cells. These results showed that SBSFBu increased levels of serum IL-4but not IL-2 and IFN-${\gamma}$, and increased expression of CD4$^+$ T cells and CD8$^+$ T cells in splenocytes in vivo, while SBSFCh increased levels of serum IL-2 and IFN-${\gamma}$ but decreased IL-4, and increased CD8$^+$ T cells but not CD4$^+$ T cells. Moreover, both of SBSFBu and SBSFCh inhibited proliferation of HL60, U937, and L1210 leukemia cell lines in a dose-dependent manner, up-regulated NO production by RAW264.7 cells in a dose-dependent manner, and enhanced expression of p53 gene in U937 leukemia cells. Our findings indicate that SBSFBu and SBSFCh may enhance T cell-dependent immune responses, and that both of SBSFBu and SBSFCh may inhibit proliferation of leukemia cells by up-regulation of NO production and expression of p53 gene.

• Proceedings of the Korean Aquaculture Society Conference
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• 2006.05a
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• pp.476-478
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• 2006

To analyze subcellular localization of betanodavirus protein B2, a plasmid expressing Betanodavirus protein B2 fused to enhanced green fluorescent protein (EGFP-Nl) was constructed. The transient expression of full-length B2 fused to EGFP in GF cells confirmed the equal distribution of protein B2 between cytoplasm and nucleus. However, transfection of N-terminal half of the B2 revealed that this truncated form predominantly localized to the cytoplasm. By using several deletion mutants and point mutants, we determined the regions and/or motif responsible for the subcellular localization of betanodavirus.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2009.11a
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• pp.173-173
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• 2009

높은 휘도를 갖는 $Eu^{2+}$가 도핑된 $Sr_3MgSi_2O_8$ 형광체는 고상반응법을 사용하여 합성하였다. 몰 비에 따른 조성을 정밀전자저울을 사용 무게를 칭량하여 에탄올을 용매로 하여 혼합, 건조 후 하소 온도는 $1000^{\circ}C$, 합성 온도는 $1200^{\circ}C$에서 $1400^{\circ}C$까지 $H_2$ 5% Ar 95% 환원분위기에서 소성 온도 별 결정상을 관찰하였고, 그 형태에 따라서는 Powder상태일 때와 Powder를 Press하여 bulk상태로써의 결정상의 변화를 연구하였다. 그 결과 분말상태와 성형을 가한 pellet의 두 가지 형태로 합성하여 비교한 결과 성형공정을 거친 시료의 경우가 분말 상태일 때 보다 좋지 못한 결정상이 관찰되었다. $Sr_3MgSi_2O_8$의 미세구조는 SEM으로 관찰하였다.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.445-446
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• 2000

Disinfection of pathogenic protozoa Giardia has been done by using $TiO_2$ prepared by hydrothermal process. $TiO_2$ suspended in a photoreactor or immobilized on the optical-fiber surface immersed in a photoreactor has been applied with the ultraviolet light. It has been shown that disinfection effect with $TiO_2/UV$ system 2 times as that with only the UV light and disinfection capability of Giardia increased with an increased $TiO_2$ concentration up to 0.1g/L in a $suspended-TiO_2$ reactor.