• The Journal of Engineering Geology
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• v.24 no.2
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• pp.237-246
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• 2014

Seasonal variations of major contaminants in groundwater around livestock burial areas in Gyeonggi province, Korea, were examined. Seven typical contamination indicators ($NO_3$-N, $NH_3$-N, chloride, pH, DO, ORP, and EC) were monitored in groundwater samples collected from 84 wells located within 60 m of livestock burial sites for the leachate plume emanating from the livestock burial sites. The monitoring results of pH, DO, ORP, and EC revealed minimal seasonal variations, providing no evidence for leachate plumes. The $NO_3$-N concentrations were below 30 mg/L and exhibited minimal seasonal fluctuations, even in the wells located close to (< 20 m) the burial sites; the $NH_3$-N and chloride concentrations also showed similar results. The contamination indicators examined in this study indicate that the observed groundwater contamination is primarily from preexisting pervasive contamination due to agricultural activities and livestock farming, not leachates derived from nearby livestock burial sites.

• Animal Bioscience
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• v.34 no.6
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• pp.957-966
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• 2021

Objective: Ovarian follicular development, which dependent on the proliferation and differentiation of granulosa cells (GCs), is a complex biological process in which miRNA plays an important role. Our previous study showed that miR-458b-5p is associated with ovarian follicular development in chicken. The detailed function and molecular mechanism of miR-458b-5p in GCs is unclear. Methods: The luciferase reporter assay was used to verify the targeting relationship between miR-458b-5p and catenin beta-1 (CTNNB1), which is an important transcriptional regulatory factor of the Wnt/β-catenin pathway. The cell counting kit-8 (CCK-8) assay, flow cytometry with propidium iodide (PI) and annexin V-fluorescein isothiocyanate (FITC) labeling were applied to explore the effect of miR-458b-5p on proliferation, cell cycle and apoptosis of chicken GCs. Quantitative real-time polymerase chain reaction and Western blot were used to detect the mRNA and protein expression levels. Results: We demonstrated that the expression of miR-458b-5p and CTNNB1 showed the opposite relationship in GCs and theca cells of hierarchical follicles. The luciferase reporter assay confirmed that CTNNB1 is the direct target of miR-458b-5p. Using CCK-8 assay and flow cytometry with PI and Annexin V-FITC labeling, we observed that transfection with the miR-458b-5p mimics significantly reduced proliferation and has no effects on apoptosis of chicken GCs. In addition, miR-458b-5p decreased the mRNA and protein expression of CD44 molecule and matrix metallopeptidase 7, which are the downstream effectors of CTNNB1 in Wnt/β-Catenin pathway and play functional roles in cell proliferation. Conclusion: Taken together, the data indicate that miR-458b-5p regulates ovarian GCs proliferation through Wnt/β-catenin signaling pathway by targeting CTNNB1, suggesting that miR-458b-5p and its target gene CTNNB1 may potentially play a role in chicken ovarian follicular development.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.4
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• pp.2265-2268
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• 2013

Several lines of evidence indicate that cancer is a multistep process. To survey the mechanisms involving gene alteration and miRNAs in adrenocortical cancer, we focused on transcriptional factors as a point of penetration to build a regulatory network. We derived three level networks: differentially expressed; related; and global. A topology network ws then set up for development of adrenocortical cancer. In this network, we found that some pathways with differentially expressed elements (genetic and miRNA) showed some self-adaption relations, such as EGFR. The differentially expressed elements partially uncovered mechanistic changes for adrenocortical cancer which should guide medical researchers to further achieve pertinent research.

• BMB Reports
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• v.48 no.12
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• pp.643-644
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• 2015

In eukaryotes, small RNAs play important roles in both gene regulation and resistance to viral infection. Argonaute proteins have been identified as a key component of the effector complexes of various RNA-silencing pathways, but the mechanistic roles of Argonaute proteins in these pathways are not clearly understood. To address this question, we performed single-molecule fluorescence experiments using an RNA-induced silencing complex (core-RISC) composed of a small RNA and human Argonaute 2. We found that target binding of core-RISC starts at the seed region of the guide RNA. After target binding, four distinct reactions followed: target cleavage, transient binding, stable binding, and Argonaute unloading. Target cleavage required extensive sequence complementarity and accelerated core-RISC dissociation for recycling. In contrast, the stable binding of core-RISC to target RNAs required seed-match only, suggesting a potential explanation for the seed-match rule of microRNA (miRNA) target selection.

• Proceedings of the SAREK Conference
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• 2006.11a
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• pp.100-100
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• 2006

• The Korean Journal of Pain
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• v.26 no.3
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• pp.295-298
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• 2013

Glomus tumors are benign tumors that account for 1% to 5% of all soft tissue tumors of the hand and are characterized by a triad of sensitivity to cold, localized tenderness and severe paroxysmal pain. Paroxysmal pain is a symptom common not only in glomus tumors but also in CRPS, and the hand is one of the commonly affected sites in patients with both glomus tumors and CRPS. Therefore, it is not easy to clinically diagnose glomus tumors superimposed on already affected region of CRPS patients. We report a case of glomus tumor concomitantly originating with CRPS at the hand.

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.220-221
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• 2003

Bacterial infection is a very complex process in which both pathogens and host cells play crucial roles, and the host cells undergo drastic changes in their physiology, releasing various proteins in response to the pathogenic infection. Human airway epithelial surface serves as a first line of defense against microorganisms and the external environment. It is well known that bronchial epithelial cells secrete various chemokines and cytokines such as IL-6 and IL-8 to cope with various respiratory pathogens. (omitted)

• Animal Systematics, Evolution and Diversity
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• v.30 no.1
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• pp.22-25
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• 2014

Two species of small gobiid fishes genus Luciogobius, i.e., L. elongatus, L. platycephalus were described as the first records from Korea based on specimens collected from gravel beaches of Gijang-gun, Busan-si, Korea. Luciogobius elongatus is distinguished from congeners by very elongated and naked body, 42-44 vertebrae, 6-7 dorsal soft rays, and no free rays on pectoral fin, and L. platycephalus is characterized by having very depressed head, naked body, 40-41 vertebrae, 10-11 dorsal soft rays, and pectoral fin with only upper 2-4 free rays. We propose the new Korean name "Ga-neun-mi-kkeun-mang-dug", "Nab-jak-mi-kkeun-mang-dug" for L. elongatus and L. platycephalus, respectively.

• Journal of electromagnetic engineering and science
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• v.9 no.2
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• pp.53-60
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• 2009

Remarkable developments in the electric and electronic industry have given people's daily life much more convenience and abundance. However, in electrical communication, or electric and electronic fields, making electrical and electronic devices more functional, more integrated, and faster have put electromagnetic interference(EMI) into more complex forms, and lots of problems such as interruption, malfunction, or interference by noise between electrical communication systems occurs every day. In this research, the electromagnetic compatibility(EMC) filter in the high powered breaker was designed and fabricated as a counter measure. The filter attenuated noise more than 20${\sim}$50 dB in the range of 10 MHz${\sim}$1.5 GHz. And, when the electric fast transient(EFT) of 4 kV in the level 4 of IEC 61000-4-4 was induced, it was soon suppressed to 600 V.

• Proceedings of the Korean Geotechical Society Conference
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• 2001.03a
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• pp.49-56
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• 2001

The techniques to make assessment of the structural integrity of underground structures include Infrared thermagraphy, GPR using the reflection of the electromagnetic wave, ultrasonic test, seismic methods using the propagation of elastic wave, and etc These methods have pros and cons in the assessment of the structural integrity in the complex environment of the underground structure, so that a single method alone is not enough to evaluate parameters required for the assessment. In this study, a new seismic method was proposed to improve the existing methods and to provide an additional information like stiffness of concrete. The proposed method combines the advantages of the modified impact-echo test and the SASW method. To verify the validity of the proposed method, a large scale model of a tunnel concrete liner was built and the proposed method was applied to the center of the model and also to the corner of the model which has several distinct reflection boundaries.