• Applied Biological Chemistry
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• v.34 no.4
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• pp.366-372
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• 1991

The volatile components, normal hydrocarbons, organic acids, and nonvolatile fatty acids in the various parts of Luffa cylindrica(L.) Roemer(sponge-gourd) were analyzed by GC and GC/MS. More than 150 volatile components were separated and thirty seven components were identified and quantified. The contents of essential oil were 0.05%, 0.05%, and 0.08% on a dried weight base in leaves, stem, and seeds respectively. Fruit juice and sap contained 0.06% and 0.03% oil on the fresh weight base. Twenty six components of normal hydrocarbons in leaves, stem, seed, and unripe fruit were separated, identified, and quantified. The total concentrations of the hydrocarbons were $75.5\;{\mu}g/g$ in leaf, $52.0\;{\mu}g/g$ in stem, $46.6\;{\mu}g/g$ in fruit juice, and $32.8\;{\mu}g/g$ in seed fractions. The major hydrocarbons in leaves, stem, and fruit juice were $nC_{25}$, $nC_{27}$, $nC_{29}$, and $nC_{31}$, $nC_{16}$, $nC_{17}$, $nC_{18}$, and $nC_{19}$ were abundant in seeds mainly. The concentration of malonic acid among the five organic acids was highest in leaves, stem, and flowers. Unripe fruit contained 24.5 mg/g of the five organic acids and malic and citric acids were higher. The concentrations of palmitic, linoleic, and linolenic acids were higher concentration in the various parts of sponge-gourd and palmitic acid was distributed in the most parts. The concentrations of organic and fatty acids in the sap were negligible.

• Food Science and Preservation
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• v.21 no.5
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• pp.694-701
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• 2014

The quality characteristics of blanched Aster scaber with the blanching condition and various solutes (non-treated, NT; soluble Ca, SC; sodium bicarbonate, SB; and magnesium sulfate, MS) were evaluated with different blanching times and solutes. The blanching process decreased the total polyphenolics, ascorbic acid contents, hardness, and cutting strength of the leaves. As for the pectinesterase and polygalacturonase, the blanching process increased their inhibitory activities, and more than 90% of them were inactivated in all the samples that were blanched. For the total counts and the number of coliform groups, the number of total aerobes at 5.92-log CFU/g before the blanching process was reduced to the approximately 2-3 log scale, and the coliform group was not detected after the blanching. The blanching also significantly decreased the total chlorophyll a and b ratios. The sensory characteristics of the Aster scaber according to the test group showed that the leaves blanched for 3 min were the most highly evaluated in terms of their overall acceptability. The phenolic compound, chlorophyll, and carotenoid contents tended to increase from before their blanching, and the Ca/Cb ratio was higher in the SC. These results showed that SC and MS treatment had greater effects on the quality characteristics and the pigmentation.

• Applied Biological Chemistry
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• v.35 no.2
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• pp.99-105
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• 1992

Artemisia annua contains the antimalarial principle, artemisinin. The possibility of the production of this compound through tissue culture technique was studied. The optimum combinations of hormones for the induction of callus were p-chlorophenoxyacetic acid(pcPA) and 6-benzylaminopurine(BAP) or pcPA and N-isopentenylaminopurine(2iP) in 0.05 mg/l each. For the growth of callus, the same combination of pcPA and BAP was optimum in concentrations of $1.0\;{\mu}M\;and\;0.5\;{\mu}M$, respectively, and the optimal concentration of sucrose was also found to be 2%(w/v). Tissue culture from the crown gall grew faster than normal callus. In the suspension culture broth and the cells of normal callus or Agrobacterium-transformed tumors, arteannuic acid and 11,12-dihydroarteannuic acid were found together with common phytosterols, whereas artemisinin was not found.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.24-24
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• 2018

본 연구는 응달고사리[Cornopteris crenulatoserrulata (Makino) Nakai]의 전엽체 대량증식 및 포자체 형성을 위한 배지구성물질 및 배양토의 최적조건을 구명하고자 수행되었다. 실험재료는 기내에서 포자발아를 통해 획득한 전엽체를 계대배양하여 실험에 사용하였다. 배지구성물질이 전엽체의 증식 및 형태 형성에 미치는 영향을 알아보고자, 배지구성물질의 농도를 1/4, 1/2, 1배로 조절한 MS배지와 Knop배지를 조성하였다. 기내 배양된 전엽체 300mg을 메스로 다진 후 상기 5종의 배지에 배양하였다. 실험의 결과, 전엽체의 생체중은 Knop배지에서 3.93g으로 가장 많이 증가되었으며, 현미경으로 관찰한 결과 기관형성도 정상적으로 이루어졌다. 선행연구에서 선발된 Knop배지를 기준으로 sucrose(0, 0.5, 1.0, 2.0 및 3.0%) 및 활성탄(0, 0.1, 0.2 및 0.4%)의 농도를 달리 첨가하여 전엽체에 미치는 영향을 확인하였다. 실험결과, 0.5%의 sucrose를 첨가한 배지에서 생체중이 5.90g으로 증가하였으며, 활성탄은 첨가하지 않는 것이 효과적이었다. 포자체 형성을 위한 최적의 토양조건을 구명하고자, 원예상토와 피트모스, 펄라이트, 마사토 등 인공토양의 비율을 달리하여 8종류의 토양을 사각분에 충진하였으며, 기내에서 배양된 전엽체를 10초간 분쇄한 후 사각분에 1g씩 균일하게 분주하여 16주간 배양하였다. 배양결과, 원예상토를 단용으로 처리한 실험구에서 포자체가 171.5개로 가장 많이 발생하였으며, 원예상토와 마사토를 2:1(v:v)로 혼합한 토양에서 125.0개, 원예상토, 피트모스, 마사토를 1:1:1(v:v:v)로 혼합한 토양에서 113.3개 순으로 포자체가 발생하였다. 포자체의 생육은 포자체의 형성이 가장 많았던 원예상토를 단용한 토양에 비해 원예상토와 마사토를 2:1(v:v)로 혼합한 토양에서 생체중, 엽장, 엽폭, 근장 등이 우수하였다. 한편 피트모스가 혼합된 토양에서는 전엽체와 포자체 형성이 억제되는 경향을 보였다. 위 결과로 보아 응달고사리의 전엽체 기내배양에는 sucrose 0.5%가 첨가된 Knop배지가 적합하며, 포자체 대량생산을 위해서는 원예상토를 단용한 토양을 사용하는 것이 좋다고 판단되었다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.22-22
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• 2018

실고사리[Lygodium japonicum (Thunb.) Sw.]는 국내 자생하는 덩굴성 양치식물로 유인재배가 용이하여 실내 외 조경 및 관상소재로 활용이 가능하다. 한방에서는 전초를 해금사초, 포자를 해금사, 뿌리와 지하경을 해금사근이라 약재로 사용한다. 본 연구는 관상 및 약재로 이용이 가능한 실고사리의 대량생산을 위한 기내 외 번식방법을 개발하고자 수행되었다. 식물재료는 경상북도 의성군 일대에서 성체를 수집하여 청주의 일반하우스에 식재하여 성숙한 포자를 채취하였다. 포자를 기내 발아시켜 전엽체를 획득한 다음 8주 간격으로 계대하면서 연구의 재료를 확보하였다. 전엽체의 증식과 생육에 적합한 배지를 비교 하고자, 1/4, 1/2, 1, 2MS배지와 Knop배지를 조성하여 배양하였다. 배양방법은 전엽체 300mg을 메스로 균일하게 다져서 배양하는 방법을 이용하였으며, 배양환경은 온도($25{\pm}1.0^{\circ}C$), 광도($30{\pm}1.0{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$), 광주기(16/8h light/dark)로 조절되었다. 연구결과, 모든 처리구의 생체중은 초기접종량 보다 11배 이상 증가하였고, 그 중 1MS배지에서는 전엽체의 생체중이 7.3g으로 가장 많이 증가하였다. 뿐만 아니라, 형태형성도 우수하여 모두 정상적인 전엽체의 형태인 하트형으로 발달이 유도되었다. 전엽체로부터 포자체 형성을 유도하고자, 원예상토, 피트모스, 펄라이트 및 마사토의 혼합비율을 5종류로 달리하여 사각분($7.5{\times}7.5{\times}7.5cm$)에 혼합토양을 충진하였다. 전엽체 1g과 증류수 25mL를 핸드블랜더로 10초간 분쇄하여 사각분의 토양표면에 균일하게 분주하는 방법을 사용하였다. 이후 온도($25{\pm}1.0^{\circ}C$), 광도($43{\pm}2.0{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$), 광주기(16/8h light/dark), 습도($72{\pm}2.0%$)를 유지하면서 10주간 재배되었다. 연구결과, 원예상토 단용, 원예상토와 펄라이트 및 마사토가 2:1(v:v)로 혼합된 토양에서 각 498.0, 402.5, 482.5개의 포자체가 형성되어 사각분 면적대비 7.16개($cm^2$)가 생산되었다. 한편 포자체의 생육은 원예상토와 펄라이트가 2:1(v:v)로 혼합된 토양에서 생체중, 엽장, 엽폭, 근장 및 SPAD value 등이 우수하였다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.23-23
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• 2018

본 연구는 참우드풀과 우드풀의 중간형으로 알려진 좀우드풀(Woodsia intermedia Tagawa)의 전엽체 증식 및 포자체 형성을 위한 기내 외 번식조건을 구명하고자 수행되었다. 실험재료는 포자를 발아시켜 획득한 전엽체를 8주 간격으로 계대배양하면서 확보하였다. 전엽체 증식에 적합한 배양조건을 탐색하고자, 전엽체 300mg을 다진 후 배지종류(Knop, 1/4, 1/2 및 1MS)와 배지구성물질(sucrose와 활성탄)의 농도를 달리하여 8주간 배양하였다. 배양실은 온도 $25{\pm}1.0^{\circ}C$와 광도 $30{\pm}1.0{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$(16/8h)로 조절되었다. 연구결과, Knop배지에서 생체중이 2.4g으로 전엽체의 증식이 가장 왕성하였다. 한편 MS계열 배지는 농도에 관계없이 매우 저조한 증식을 보였다. Sucrose는 0.5%를 첨가한 배지에서 생체중의 증가량이 가장 컸으며, 활성탄은 첨가농도에 관계없이 유사한 증식수준을 나타냈다. 포자체 형성에 적합한 토양조건을 확인하고자, 인공토양(원예상토, 피트모스, 펄라이트 및 마사토)의 비율을 달리하여 5종류의 혼합토양을 조성하였다. 혼합토양을 사각분($7.5{\times}7.5{\times}7.5cm$)에 충진하고, 준비된 전엽체 1g을 10초간 분쇄한 다음 토양표면에 분주하여 11주간 재배하였다. 재배환경은 온도 $25{\pm}1.0^{\circ}C$, 광도 $43{\pm}2.0{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$(16/8h) 및 습도 $72{\pm}2.0%$로 조절되었다. 연구결과, 모든 처리구에서 포자체의 형성이 확인되었으며, 특히 원예상토와 마사토를 2:1(v:v)로 혼합한 토양에서 포트당 421.0개의 많은 포자체가 형성되었다. 다음으로 원예상토와 펄라이트를 2:1(v:v)로 혼용한 토양, 원예상토, 피트모스, 마사토를 1:1:1(v:v:v)로 혼합한 토양, 원예상토 단용, 원예상토, 피트모스, 펄라이트를 1:1:1(v:v:v)로 혼합한 토양 순으로 각 228.0, 203.3, 126.8, 91.5개 형성되었다. 따라서 좀우드풀의 포자체를 대량으로 생산하기 위해서는 원예상토와 마사토를 2:1(v:v)로 혼용한 토양에 전엽체를 분주하여 재배하는 방법이 효과적일 것으로 판단된다.

• The Korean Journal of Community Living Science
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• v.28 no.4
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• pp.525-535
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• 2017

The purpose of this study was to compare the of ash and minerals contents in different types of soybeans (Baektae, Seoritae, Heuk Tae and Seomoktae) with different cooking methods. Raw, boiled and pan-fried soybean samples were prepared by the Rural Development Administration. Samples were digested by using a microwave and analyzed using ICP-OES and ICP-MS for determining the ash and minerals contents. Ash content in four raw soybeans ranged from 5.07 to 5.59 g/100g. There was no significant difference in the ash contents in raw and pan-fried soybeans (p>0.05). However, the ash content of boiled soybeans was 9 to 14% lower than that of raw soybeans (p<0.05). The minerals contents of Baektae and Seomoktae were high and the major minerals of various soybeans were K, P, Mg, and Ca, which accounted for ash 60.80-65.61%, 20.73-24.26%, 6.90-8.46% and 5.79-8.60%, respectively. The mineral contents of soybeans were significantly different depending on the type of cultivar (p<0.05), but the tendency was not constant. Because the process of soaking and boiling of soybeans made the soluble minerals elute, the ash and mineral contents were reduced. And process of pan-frying of soybeans make Because process of soaking and boiling of soybeans make soluble minerals elute (Ed- this section is very confused and seems to have 2 sentences combined: please check your intended meaning), the ash and mineral contents were reduced. As the soybeans are cooked by pan-frying, the water in the soybeans is evaporated and the mineral content is concentrated or kept constant. These results can serve as the basic data of mineral content during processing and cooking of soybeans.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.6
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• pp.885-891
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• 2005

The study was carried out to find the effect of freeze drying on the volatile organic components in garlic (Allium sativum L.). The volatile organic compounds from fresh and freeze dried garlic were extracted by simultaneous steam distillation and extraction (SDE) method and identified with GC/MS analysis. A total of 42 and 32 compounds were identified in fresh and freeze-dried garlic, respectively. Sulfur containing compounds in the garlic samples were detected as the major compounds, and alcohols, aldehydes and esters were detected as minor compounds. Diallyl disulfide, diallyl trisulfide, allyl methyl disulfide and ally1 methyl trisulfide were the main sulfur compounds in fresh and freeze dried garlic. The amount of sulfur containing compounds were decreased freeze-drying but methyl propyl trisulide, 3- allylthiopropionic acid, cyclopentyl ethyl sulfide etc. were increased. The others, non- sulfur containing compounds such as ethyl acetate, ethanol, 2-propenol, 2- propenal and hexanal were increased in freeze-dried garlic. Consequently, the total amount of volatile organic compounds in garlic became lower during freeze-drying from 853.42 mg/kg to 802.21 /kg, and the composition of major components were nearly same in fresh and freeze-dried garlic.

• Korean Journal of Plant Tissue Culture
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• v.28 no.2
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• pp.103-108
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• 2001

The in vitro plantlets of cassava (Manihot esculenta Crantz cv. MColl 22) could be regenerated from nodal explant cultures in a liquid MS basal medium containing 0.01 mg/L zeatin for 2 weeks. The plantlets of 1.5∼2.5 cm in shoot length were transplanted to a glass bottle containing fine sand and acclimated under non-sterile conditions after excising their intact roots by: 1) prune leaving roots base of 1∼1.5 cm; 2) complete removal of roots; and 3) cutting off the rooting zone. The majority of in vitro-formed intact roots continued growth after transferred to soil, and all of the damaged roots stopped further growth. The plantlets with excised roots began to develop new roots within 7∼10 days after being transferred to a glass bottle, and a few of the pruned roots developed lateral roots from the remaining portion. Pruning and removal of in vitro roots resulted in a high survival rate (>87%), and did not significantly affect ex vitro root regeneration and acclimation, but the plantlets in which the rooting zone had been cut-off showed 73% survival rate. Pruning or removal of in vitro roots before transfer of plantlets is recommended for useful method of commercial micropropagation because of easier handling and high survival rate of plantlets.

• Korean Journal of Food Science and Technology
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• v.46 no.5
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• pp.630-635
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• 2014

Pine nut oil (PNO) is well known to impart beneficial effects in overweight individuals, but the mechanisms underlying PNO-mediated weight loss remain unclear. To investigate how PNO promotes weight loss, its composition was determined by gas chromatography coupled with mass spectrometry (GC-MS). In addition, the effects of PNO on cytotoxicity, lipid accumulation, expression of lipid metabolism-related biomarkers, and leptin secretion were assessed in 3T3-L1 cells. GC-MS analyses revealed that PNO contains several components, including linoleic acid, oleic acid, palmitic acid, and stearic acid. Moreover, PNO did not have a cytotoxic effect on 3T3-L1 cells. However, it inhibited the expression of peroxisome proliferator-activated receptor (PPAR) and adipocyte protein 2 (aP2). Finally, PNO significantly increased leptin secretion in a dose-dependent manner. Taken together, these results support the notion that PNO is useful for weight management in overweight individuals.