• Restorative Dentistry and Endodontics
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• 제32권5호
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• pp.459-468
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• 2007

이 연구에서는 법랑모세포 분화과정에서 APin의 기능을 알아보고자 APin-protein microarray를 시행한 후 치아발생과 관련이 있는 MEF2, Aurora kinase A, BMPR-IB와 EF-hand calcium binding protein을 분석하여 다음과 같은 결과를 얻었다. 1 CMV-APin construct를 transfection하여 APin의 과발현을 유도한 경우에는 MEF2와 Aurora kinase A 둘 모두에서 발현이 현저히 감소한 반면에, APin의 발현억제를 유도한 경우에는 둘 모두 변화가 없었다. 2. APin의 과발현을 유도한 경우에는 BMPR-IB와 EF-hand calcium binding protein 모두에서 발현이 크게 증가한 반면, APin을 발현억제 시킨 경우에는 BMPR-IB는 변화가 없었고, EF-hand calcium binding protein은 현저히 감소하였다. 위의 결과들로 보아 APin 단백질은 MEF2, Aurora kinase A, BMPR-IB, EF-hand calcium binding protein과 상호작용하여 법랑모세포의 분화와 석회화 과정 중에 중요한 역할을 하는 것으로 사료된다.

• 생명과학회지
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• 제14권1호
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• pp.82-90
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• 2004

곰보버섯을 약용과 식용으로 이용하고자 일반성분, 무기질, 총아미노산, 유리 아미노산, 비타민 등을 분석 검토한 결과 조지방이 3.8 g%, 탄수화물이 43.5 g%, 조단백질이 29.7 g%이었다. 무기물은 K가 3558.0 mg%로 가장 많이 함유되어있고, Ca, Mg, Fe, Na, Zn의 순으로 함유되었다. 총 아미노산은 glutamic arid가 1,433 mg%로 가장 많이 함유되어있고, leucine, alanine, arginine, valine, threonine등의 순으로 23종의 아미노산을 함유하고 있으며 필수아미노산은 3510mgmg% 함유되어 있다. 유리아미노산은 glutamic acid가 522 $\mug%$로 가장 많이 함유되어 있고, asparaginine, arginine등의 순이며, 총 함유량은 2,397 $\mug%$이고, 25종을 함유하고 있기 때문에 곰보버섯의 맛에 영향을 미치리아 생각되어 진다. 비타민의 경우 vitamin A가 2.23$\mug%$, vitamin $B_1$은 0.13 mg%, vitamin $B_2$는 0.07 mg%, vitamin $B_6$는 0.27 mg%, vitamin C는 0.17 mg%, vitamin $D_311$는 52.27$\mug%$, vitamin E는 5.26 mg%, vitamin$K_1$, 은 3.23 $\mug%$정도 함유하고 있었다. 특히 vitamin C와 vitamin E가 함유되어 있어 노화방지에 좋으리라 생각된다.

• 대한한방부인과학회지
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• 제25권2호
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• pp.78-88
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• 2012

Objectives: Paeonia japonica has been widely used for gynecopathy and analgesic effects in Korean Traditional Medicine. The aim of the present study is to determine the antioxidant effect of Paeonia japonica extracts(PJE) by using mouse embryonic fibroblast cells(MEF cells). Methods: We evaluated Radical Scavenging Activity of PJE by the DPPH assay. Protective effect of the PJE on the hydrogen peroxide($H_2O_2$) induced oxidative damage of MEF cells was analyzed by the MTT assay. The Morphological changes of MEF cells induced by P. japonica, $H_2O_2$ and P. japonica+$H_2O_2$ was evaluated by DAPI staining. And effect of PJE on the rate of apoptosis in MEF cells was measured using flow cytometry with Annexin V-FITC and PI double staining. Results: We observed that PJE contain significant DPPH radical scavenging activity. Cell viability of oxidative damaged cells treated with various concentrations of $H_2O_2$ was increased by treatment with PJE. Flow cytometric analysis of the cells treated with $H_2O_2$ in the absence or presence of PJE showed that the crumbled G1 peak was accumulated by the treatment with $H_2O_2$ alone, but restored by addition of PJE. Portion of cells that undergo apoptosis mediated by oxidative stress was decreased by treatment of PJE. The nuclear fragmentation occurred in the oxidative damaged MEF cells was also decreased by PJE treatment. Conclusions: Taken together, our results suggest that PJE exhibits significant antioxidant activity and functions to inhibit cell death mediated by oxidative damage induced apoptotic pathways.

• 대한의생명과학회지
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• 제18권2호
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• pp.165-168
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• 2012

Hox proteins containing DNA-binding homedomain act as transcription factors important for anteroposterior body patterning during vertebrate embryogenesis. However, the precise mechanisms by which signal pathways are transduced to regulate the Hox gene expression are not clear. In the course of an attempt to isolate an upstream regulatory factor(s) controlling Hox genes, protein kinase B alpha (Akt1) has been identified as a putative regulator of Hox genes through in silico analysis (GEO profile). In the Gene Expression Omnibus (GEO) dataset GDS1784 at the NCBI (National Center for Biotechnology Information) site, Hox genes were differentially expressed depending on the presence or absence of Akt1. Since it was not well known how Akt1 regulates the specific Hox genes, whose transcription was reported to be regulated by epigenetic modifications such as histone acetylation, methylation etc., the expression of Gcn5, a histone acetyltransferase (HAT), was analyzed in wild type (WT) as well as in $Akt1^{-/-}$ mouse embryonic fibroblast (MEF) cells. RT-PCR analysis revealed that the amount of Gcn5 mRNA was similar in both WT and $Akt1^{-/-}$ MEFs. However, the protein level of Gcn5 was significantly increased in $Akt1^{-/-}$ MEF cells. The half life of Gcn5 was 1 hour in wild type whereas 8 hours in $Akt1^{-/-}$ MEF. These data all together, indicate that Gcn5 is post-transcriptionally down-regulated and the protein stability is negatively regulated by Akt1 in MEF cells.

• Nutrition Research and Practice
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• 제13권2호
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• pp.95-104
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• 2019

BACKGROUND/OBJECTIVES: Inflammatory Bowel Disease (IBD) has rapidly escalated in Asia (including Korea) due to increasing westernized diet patterns subsequent to industrialization. Factors associated with endoplasmic reticulum (ER) stress are demonstrated to be one of the major causes of IBD. This study was conducted to investigate the effect of Lycium barbarum (L. barbarum) on ER stress. MATERIALS/METHODS: Mouse embryonic fibroblast (MEF) cell line and polarized Caco-2 human intestinal epithelial cells were treated with crude extract of the L. chinense fruit (LF). Paracellular permeability was measured to examine the effect of tight junction (TJ) integrity. The regulatory pathways of ER stress were evaluated in MEF knockout (KO) cell lines by qPCR for interleukin (IL) 6, IL8 and XBP1 spliced form (XBP1s). Immunoglobulin binding protein (BiP), XBP1s and CCAAT/enhancer-binding homologous protein (CHOP) expressions were measured by RT-PCR. Scanning Ion Conductance Microscopy (SICM) at high resolution was applied to observe morphological changes after treatments. RESULTS: Exposure to LF extract strengthened the TJ, both in the presence and absence of inflammation. In polarized Caco-2 pretreated with LF, induction in the expression of proinflammatory marker IL8 was not significant, whereas ER stress marker XBP1s expression was significantly increased. In wild type (wt) MEF cells, IL6, CHOP and XBP1 spliced form were dose-dependently induced when exposed to $12.5-50{\mu}g/mL$ extract. However, absence of XBP1 or $IRE1{\alpha}$ in MEF cells abolished this effect. CONCLUSION: Results of this study show that LF treatment enhances the barrier function and reduces inflammation and ER stress in an $IRE1{\alpha}$-XBP1-dependent manner. These results suggest the preventive effect of LF on healthy intestine, and the possibility of reducing the degree of inflammatory symptoms in IBD patients.

• 대한한방부인과학회지
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• 제22권3호
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• pp.36-50
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• 2009

Purpose: This study was to determine the protective effects of Dioscoreae Rhizoma on the Mouse Embrio Fibroblast (MEF) cells exposed to the ultraviolet rays(UVA). Methods: The samples were assigned randomly to five groups; control group without any treatments, UVA group exposed only to UVA, DR group exposed only to the Dioscoreae Rhizoma, UVA-DR group exposed to UVA before being treated with the Dioscoreae Rhizoma, and DR-UVA group treated with the Dioscoreae Rhizoma before being exposed to UVA. The survival rate of cells, metabolic rate of cells, transformation of nucleus within cells, alteration of cell cycle, effects on the apoptosis, the change of the amount of protein related to cell cycle were measured in order to determine the cell protective effects of the Dioscoreae Rhizoma on each group. Results: 1. DR-UVA group has more cell protective effects compared to the UVA group in all experiments, indicating that the Dioscoreae Rhizoma protects skin from UVA physically and chemically. 2. UVA-DR group shows more efficiency compared to UVA group in rapid recovery of damaged cell and leading highly damaged cells to apoptosis, preventing the expression of abnormal cells. Conclusions: Dioscoreae Rhizoma has effects of protecting MEF cells from UVA, of recovering cells damaged by UVA, and of prohibiting the expression of abnormal cells.

• 한국발생생물학회지:발생과생식
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• 제21권3호
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• pp.335-342
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• 2017

While adipose-derived stem cell-conditioned medium (ADSC-CM) has been demonstrated to promote skin wound healing, the mechanism regulating this effect remains unelucidated. In this study, we aimed to investigate the role of Ell3 in the wound healing activity of ADSC-CM. In vitro analysis revealed that Ell3 suppression in ADSCs impairs the promotive activity of ADSC-CM on the proliferation and migration of mouse embryonic fibroblasts (MEF) and normal human dermal fibroblasts (NHDF). Consistently, the expression of MMP family genes, which regulate cell proliferation and migration, was significantly suppressed in MEF and NHDF treated with siEll3-transfected ADSC-CM. Proinflammatory cytokines, such as interleukin-1 and interleukin-6, were highly expressed in MEF treated with siEll3-transfected ADSC-CM. The wound healing activity of siEll3-transfected ADSC-CM was significantly lower than that of the control in vivo. Our results suggest that Ell3 may contribute to the inhibition of inflammatory response during skin wound healing.

• 한국인터넷방송통신학회논문지
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• 제12권6호
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• pp.297-302
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• 2012

이동 백홀 망에서 RAN은 이동통신 기지국을 이동 백홀망에 연결시켜 준다. 이 시스템은 여러 세대의 이동 통신 기술에 따른 인터페이스, 즉 셀 사이트 영역에서는 TDM, ATM, 그리고 이더넷, 이동 백홀 영역에서는 이더넷 인터페이스로 구성된다. 조만간에 TDM 기반의 이동통신 네트워크는 모두 IP/Ethernet으로 전환될 것이고, 궁극적으로 이더넷 및 TDM 트래픽이 IP 네트워크를 통하여 전달될 것 이다. 따라서 이들 기술들을 이동 백홀 망으로 전송되기 위하여 각각은 캡슐화가 되어야 한다. 본 논문에서는 각각의 캡슐화, 즉, ATM, MPLS, IP/UDP, 그리고 MEF8에 따른 성능이 측정되었다.

• 생약학회지
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• 제45권2호
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• pp.127-134
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• 2014

A great interest is emerging about green tea as a tool against human cancer proliferation or inflammation, as pointed out by recent reports describing the inhibitory action of epigallocatechin gallate (EGCG) on angiogenesis, urokinase, metalloproteinases, and induction of inducible nitric oxide synthase. We proposed that EGCG may regulate a multi target signaling having wider spectra of action than those actions of single enzymes. CSK (c-terminal Src kinase) protein is a non-receptor tyrosine kinase involved in the cross-talk and mediation of many signaling pathways that promote cell proliferation, adhesion, invasion, migration, and tumorigenesis. Based on the knowledge that CSK activation is important for cancer proliferation we hypothesized that CSK could be a target of EGCG. Here we showed that EGCG effectively suppressed the growth of CSK MEF cell when compare with CSK knockout MEF cell growth. These results indicate that EGCG could be used as a chemoprevention to modulate CSK signal pathway in inflammatory processes and tumor formation.

• Natural Product Sciences
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• 제2권1호
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• pp.29-36
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• 1996

This study was to evaluate the antitumor and immunopotentiation effects of Manda Enzyme (ME). Oral administration of ME (0.2ml/mouse) to tumor bearing mice significantly prolonged survival rate compared to the control group with the prolongation ratio of 40%. The inhibition ratios for the first and the second experiments were 51.8% and 26.4%, respectively. Only the spleen index was significantly increased in the MEF-treated group, but not in the control group. Gamma globulin level of the MEF-treated group was elevated when mice were injected with sarcoma-180 cells on the left groin. Activities of natural killer (NK) and lymphokineactivated killer (LAK) cells were observed by $^{51}Cr-release$ method. Activities of NK cell against YAC-1 cells were significantly increased in the MEF treated group. And LAK cell activities against P815 cells were also significantly increased in the experimental group. These observations, therefore, suggest that ME may have an anticancer effect and immunopotentiating effect in vivo.