• Food Science and Preservation
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• v.14 no.5
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• pp.445-450
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• 2007

This studies were engaged to investigate the potential us of Curcuma aromatica Salab. as a food ingredients. The antimicrobial activity of Curcuma aromatica Salab. extract (CE) and effect on shelf life of the cooked rice were examined. The antimicrobial activity of CE showed apparently against Bacillus cereus, B. subtilis and B. megaterium. The minimum inhibitory concentration (MIC) of CE was 0.15% for B. cereus md 0.2% for B. subtilis and B. megaterium. The antimicrobial activity of CE remained relatively high after heat treatments(30 min at 80 and $100^{\circ}C$, 15 min at $121^{\circ}C$). The shelf life of cooked rice containing above 0.05% CE increased more 1 or 2 days than that of control at $30^{\circ}C$, The texture of cooked rice was improved by addition of above 0.01% CE. The sensory quality of cooked rice with CE did not show significant difference between 0.00% and 0.05% in taste and overall acceptability but decreased above 0.10% in taste, color, flavor and overall acceptability.

• The korean journal of orthodontics
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• v.34 no.2 s.103
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• pp.143-152
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• 2004

It has not been elucidated whether the initiation of condylar development of the mandible is related with the periosteum of the mandible, or if it derives from a separate programmed blastema not related with the mandible. Also, although the mandibular condylar cartilage is known to promote growth, few studies have dealt with molecular-biologic mechanisms such as the expression of specific genes according to the differentiation of the mandibular condyle. To elucidate the unique cellular characteristics, development, and differentiation process of the mandibular condyle, an examination of expressions of genes characteristic of cartilage and bone were carried out using RT-PCR and mRNA in situ hybridization. 1. Type? collagen mRNA was detected with type II collagen mRNA in the differentiation and growth process of the cartilage of the mandibular condyle. TypeII collagen mRNA was demonstrated in the whole resting md upper part of the poliferative zone, whereas type II collagen mRNA was observed in the resting, proliferative and upper hypertrophic cartilage zone of the mandibular condyle. 2. The condylar cartilage rapidly increased in size due to the accumulation of hypertrophic chondrocytes as characterized by the expression of type II collagen mRNA during postnatal development. 3. BMP-4 mRNA was present in the anlage of the future condylar process and also in the ossifying mandibular body. 4. IHH mRNA was limited exclusively to the lower part of the proliferative zone and the upper part of the hypertrophic cartilage zone during condylar development. These findings were different from those in the growth-plate cartilage of the long bone, indicating a characteristic feature of the differentiation of the chondrocytes in the condylar cartilage present in prenatal and postnatal development. Furthermore, it was also suggested that chondroblasts of condylar cartilage rapidly differentiate into hypertrophic chondrocytes with increased functional Load force such as muscle activity and mastication.

• Journal of the Korean Home Economics Association
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• v.40 no.12
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• pp.85-108
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• 2002

The objectives of this study were to investigate the trend of the attitude and the behavior about consumer's internet premium advertisement and its application and the factors which influence on the attitude and the behavior about consumer's premium advertisement and its application; the demographic variables, internet communication-environment variables and variables in relation to consumer's premium advertisement application. For these purpose, the data collected two way: internet survey and questionnaire survey. The data used in this study included people who have looked an internet Premium Advertisement more than once during internet use. Major findings were as following: (1) The attitude of internet premium advertisement and its application could be described as the mean 34.16 md 24.61 separately(3.11 and 2.73 point on 5.00 points scale). The behavior of application about internet premium advertisement was measured as a behavior of application whether or not; application 18.7% and unapplication 81.3%. (2) The attitude of application about internet premium advertisement was significantly different according to age, joining frequency on internet, past experience of winning a premium, purchasing information search, interesting connection on the internet, the risk about the money loss and negative evaluation of other persons, the economical values of premiums, winning possibility, and preferable premiums. The behavior of application about internet premium advertisement, the behavior of application whether or not was significantly different according to sex, age, school career, a circuit speed, a period of using, joining frequency and a using place, past experience of winning a premium, purchasing information search, interesting connection on the internet, the risk about the negative evaluation of other persons, the economical values of premiums, winning possibility, and preferable premiums. (3) When looking at the influence of variables about the attitude of application about internet premium advertisement, the most influence variables was the past experience of winning a premium, and next was winning possibility, sex, the interest about the premiums which made the consumer want to apply, the economical values of premiums, the purpose of purchase information search and the using time. The explanation of those variables were 22.6%. As the result of the Logistic analysis, it was found that the frequency of the internet connection, the past experience of winning premiums, the risk about the outflow of credit information, interesting premiums, and premium advertisement were the most important variables to affect the possibility of the behavior of application about internet premium advertisement critically.

• The Korean Journal of Zoology
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• v.36 no.4
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• pp.439-451
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• 1993

Protein kinase C isozymes in a human prostate adenocarcinoma PC-3 cell line were characterized. Immunoreactive bands and immunocytochemical stains were obsenred in PC-3 cells with antibodies raised against protein kinase C ${\alpha}$, ${\beta}$, ${\gamma}$, $\delta$, $\varepsilon$, and ζ types, respectively. Protein kinase C ${\alpha}$ corresponded to a immunoreactive band at a molecular weight of 80,000-dalton, whereas molecular weights of other immunoreactive isozvmes of protein kinase C were detected at 68,000-dalton. Protein kinHse C $\delta$ and ζ antibodies detected additional bands at 55,000-dalton and 80,000-dalton, respectively Immunocvtochemical study confirmed the results of the immunoblotting experiments qualitatively: all six protein kinase C isozymes were detected in the cytoplasm of PC-3 cells. Translocation of protein kinase C in PC-3 cells were also examined with phorbol 12-myristate 13-acetate (PMA), bryostatin 2, diolein, and 1-oleoyl-2-acetyl glycerol (OAG). Differential reactions of protein kinase C isozvmes to these activators were obsenred. When PC-3 cells were treated with 10mM bryostatin 2, protein kinase C isozyme u was translocated into the nucleus, whereas s type was translocated into the plasma membrane and the nucleus. Protein kinase C ${\alpha}$ and ζ types were translocated into the nucleus following the treatment with 101M diolein, whereas protein kinase C ${\alpha}$, ${\beta}$, ${\gamma}$, and $\varepsilon$ types were translocated into the nucleus by the treatment with 10mM OAG. Protein kinase C ${\alpha}$ and $\varepsilon$ types were translocated into the nucleus in the presence of 100nM PMA. Protein kinase C $\delta$ type was translocated to the nuclear membrane by these activators, however, only PMA-induced translocation was inhibited by protein kinase C inhibitor, 1-(5-isoquinolinesulfonyll-2-methvlpiperazine dihvdrochloride (H7) . H7 inhibited translocation of protein kinase C ${\alpha}$ type induced by PMA, ${\beta}$ type by OAG and s type by PMA and OAG, whereas it did not affect translocations induced by bryostatin and diolein, respectively. These results suggest that there exist six isoformes of protein kinase C (${\alpha}$, ${\beta}$, ${\gamma}$, $\delta$, $\varepsilon$ and ζ types) in PC-3 cells and that each of these isozvmes distinctivelv reacts to bryostatin, diolein, OAG and PMA, in part due to an altered molecular size and conceivably discrete binding site(s).

• Journal of Animal Science and Technology
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• v.60 no.11
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• pp.27.1-27.8
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• 2018

Background: Enteric methane ($CH_4$) accounts for about 70% of total $CH_4$ emissions from the ruminant animals. Researchers are exploring ways to mitigate enteric $CH_4$ emissions from ruminants. Recently, nano zinc oxide (nZnO) has shown potential in reducing $CH_4$ and hydrogen sulfide ($H_2S$) production from the liquid manure under anaerobic storage conditions. Four different levels of nZnO and two types of feed were mixed with rumen fluid to investigate the efficacy of nZnO in mitigating gaseous production. Methods: All experiments with four replicates were conducted in batches in 250 mL glass bottles paired with the ANKOM$^{RF}$ wireless gas production monitoring system. Gas production was monitored continuously for 72 h at a constant temperature of $39{\pm}1^{\circ}C$ in a water bath. Headspace gas samples were collected using gas-tight syringes from the Tedlar bags connected to the glass bottles and analyzed for greenhouse gases ($CH_4$ and carbon dioxide-$CO_2$) and $H_2S$ concentrations. $CH_4$ and $CO_2$ gas concentrations were analyzed using an SRI-8610 Gas Chromatograph and $H_2S$ concentrations were measured using a Jerome 631X meter. At the same time, substrate (i.e. mixed rumen fluid+ NP treatment+ feed composite) samples were collected from the glass bottles at the beginning and at the end of an experiment for bacterial counts, and volatile fatty acids (VFAs) analysis. Results: Compared to the control treatment the $H_2S$ and GHGs concentration reduction after 72 h of the tested nZnO levels varied between 4.89 to 53.65%. Additionally, 0.47 to 22.21% microbial population reduction was observed from the applied nZnO treatments. Application of nZnO at a rate of $1000{\mu}g\;g^{-1}$ have exhibited the highest amount of concentration reductions for all three gases and microbial population. Conclusion: Results suggest that both 500 and $1000{\mu}g\;g^{-1}$ nZnO application levels have the potential to reduce GHG and $H_2S$ concentrations.

• Strategy21
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• s.37
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• pp.242-274
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• 2015

2015년 5월 수중발사 탄도미사일(SLBM) 발사시험을 통해 북한은 전 세계의 이목을 또 다시 집중시켰다. 북한은 2013년 제3차 핵실험을 감행한 이후 최근까지도 탄도미사일과 로켓 발사 등의 무력도발을 지속해 왔다. 이와 같은 북한의 무력도발은 한반도의 안정뿐만 아니라 미국 본토의 안보에도 매우 부정적인 영향을 미치고 있다. 지금까지 미국은 북한의 핵미사일 개발 저지를 위해 경제적 또는 군사적 방안들을 선별적으로 적용해 왔다. 그러나, 이러한 미국의 노력에도 불구하고 북한은 여전히 핵미사일을 개발하겠다는 꿈을 포기하지 않고 있는 것이 사실이다. 본 논문은 북한의 핵미사일 개발에 대한 미국의 대안적 전략(Alternative Strategy)을 제시하는데 그 목적이 있다. 필자는 대안적 전략 제시에 앞서 북한의 핵과 탄도미사일 개발현황을 내용(Contents)과 맥락(Context) 차원에서 분석하고 미 오바마 행정부의 대북전략을 비판적으로 검토하였다. 그리고 대안적 전략으로 동맹국과 함께 경제적(Sticky Power), 군사적(Sharp Power) 수단의 동기화(Synchronizing)를 통해 북한이 감당하기 힘든 전략적 환경을 조성(Shaping the Strategic Environment)하는 것이 '북한의 핵개발 포기' 라는 전략목표를 달성하는 방안 임을 강조하고 있다. 미국이 대안적 전략목표를 달성하기 위해서는 경제적 수단으로 ① 북한의 자금세탁 및 위조지폐 발행국 지정, ② 북한을 지원하는 모든 해외자산에 대한 제재조치, ③ 북한의 테러지원국 재지정, ④ 북한 제재를 위한 미국의 입법추진, ⑤ 대량살상무기 관련 금수품목의 확대를 적용함과 동시에 군사적 수단으로 ① 대량살상무기 비확산 활동 강화, ② 대탄도미사일 전략 개발 및 정보(ISR: Intelligence Surveillance and Reconnaissance) 공유, 맞춤형 억제전략(TDS: Tailored Deterrence Strategy)의 구체화 등을 통한 한·미 군사 억제방안의 강화, 그리고 ③ SM-3, THAAD(Terminal High Altitude Area Defense) 등 한·미·일 3자간 MD(Missile Defense)체제의 구축 등을 동기화하여 적용해야만 할 것이다. 미국의 대안적 전략은 ① 북한의 핵미사일 개발에 대한 중국의 협력방지, ② 한·중간 경제관계의 악화, ③ 한·일간의 역사적 긴장관계라는 위험요소를 내포하고 있는 것 또한 사실이다. 따라서 미국이 대안적 전략목표인 '북한의 비핵화' 달성을 위해서는 이와 같은 위험요소를 완화시키는 노력도 병행되어야 할 것이다. 끝으로, 미국은 북한의 김정은이 핵미사일 개발 포기라는 상이한 방향의 전략적 결정을 할 경우에 대비하여 한·미 연합훈련의 보류, 북한에 대한 경제제재조치 해제 등 북한과의 협상 가능성도 열어두고 이에 대해서도 철저하게 준비해 나가야 할 것이다.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.1
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• pp.31-36
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• 1998

In order to find out the effect of supplemented diets with squid intestine on the catfish, growth and body compositions of catfish (Parasilurus asotus) were investigated for 112 days with the four experimental diets of different lipid levels. The higher lipid level of diets with squid intestine supplement was fed, the higher body weight was gained (p<0.05). HSI (Hepatosomatic Index) was the lowest in the diets with $10\%$ squid intestine. There were no significant differences in muscle md skin compositions, however the increasing lipid levels in the diets well reflected in the fatty acid pattern of skin and muscle. The percentage of n-3 polyunsaturated fatty acids such as C20 : 5 and C22 : 6 in tissues was increased according to increase of supplemented squid intestine. Saturated and n-6 fatty acid contents did not show the significant difference.

• FLOWER RESEARCH JOURNAL
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• v.18 no.1
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• pp.15-22
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• 2010

These investigations were conducted to standardize several chemical and physical environments affecting in vitro propagation of gametophytes of Climacium japonicum. Propagation of this moss species was established on basal medium containing Knop macro salts and Nitsch and Nitsch trace elements. Primary cultures were initiated from apical shoots of gametophytes. Gametophyte production was accessed using chopped gametophytes, apical shoots and basal shoots. Seven ty pes of culture media and four concentrations of total nitrogen and five strengths of sucrose were tested for in vitro gametophyte production. Light and temperature factors were also evaluated. Apical shoots were the greatest among three types explants used for gametophyte propagation. Medium containing Knop macro salts and Nitsch and Nitsch trace elements was more effective than other types of media. Higher sucrose concentrations showed a positive effect on the elongation and multiplication of gametophytes. Both nitrogen deficiency and excessiveness inhibited gametophyte growth. Light intensity variation showed highly significant changes in numbers, length and fresh weight of gametophytes. Optimum light intensity for gametophyte growth seemed to be around 3000-4000 lx. Both lower and higher temperature had a negative effect on gametophyte propagation and production. This study will provide large scale and high quality propagules, and effective moss propagation system.

• Korean Journal of Acupuncture
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• v.36 no.1
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• pp.1-18
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• 2019

Objectives : The purpose of this study was to evaluate the effectiveness and safety of acupuncture treatment for recovery of patients with vertebral compression fracture(VCF). Methods : We searched ten English and Chinese and seven Korean database up to April 2018. Randomised controlled trials(RCTs), quasi-RCTs, non-radomised Controlled Trials(CCTs) were eligible. Quasi-RCTs and CCTs were assessed only for safety assessment. Pain and adverse events were primary outcome of this review. Quality of life, dysfunction, patient satisfaction, incidence of new vertebral compression fracture were regarded as secondary outcomes. The risk of bias was assessed by two independent authors using the Cochrane risk of bias tool. Level of evidence was tabulated using the GRADE methods. Results : Of 1656 screened, 15 RCTs, 1 quasi-RCT and 3 CCTs were included. Number of participants per study ranged from 45 to 135. Most of the studies had unclear or high risk of bias and considerable heterogeneity in terms of type of intervention, comparison and time-points for outcome measurement. Compared to usual care alone, acupuncture combined with usual care showed short-term favorable results for pain relief in patients with VCF(5 studies, n=252, MD -1.05 point on a 0 to 10 point scale, 95% CI -1.45 to -0.65, $I^2=74%$). Four studies reported mild and temporary adverse events, and no serious adverse events were reported. One study descriptively reported that acupuncture was effective for improving quality of life without providing numerical outcomes. There were no reports of patient satisfaction and incidence of new VCF. Conclusions : Level of evidence is very low for the effectiveness and safety of acupuncture for pain, harms and other clinical outcomes in patients with VCF. Included studies suffered from incomplete reporting, high or unclear risk of bias and substantial heterogeneity between studies. Future high-quality RCTs are needed to assess whether acupuncture is beneficial for recovery of patients with VCF.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.1610-1610
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• 2001

Microbiological examination of silage is of little value in gauging the outcome of silage, and so chemical analysis is more reliable and meaningful indicator of quality. On the other hand chemical assessments of the principal fermentation products provide an unequivocal basis on which to judge quality. Livestock require energy, protein, minerals and vitamins from their food. While fresh forages provide these essential items, conserved forages on the other hand may be deficient in one or more of them. The aim of the conservation process is to preserve as many of the original nutrients as possible, particularly energy and protein components (Woolford, 1984). Silage fermentation is important to preservation of forage with respect of feeding value and animal performance. Chemical and bacteriological changes in the silo during the fermentation process can affect adversely nutrient yield and quality (Moe and Carr, 1984). Many of the important chemical components of silage must be assayed in fresh or by extraction of the fresh material, since drying either by heat or lyophilisation, volatilises components such as acids or nitrogenous components, or effects conversion to other compounds (Abrams et al., 1987). Maize silage dorms the basis of winter rations for the vast majority of dairy and beef cattle production in Uruguay. Since nutrient intake, particularly energy, from forages is influenced by both voluntary dry matter intake and digestibility; there is a need for a rapid technique for predicting these parameters in farm advisory systems. Near Infrared Reflectance Spectroscopy (NIRS) is increasingly used as a rapid, accurate method of evaluating chemical constituents in cereals and dried forages. For many years NIRS was applied to assess chemical composition in dry materials (Norris et al., 1976, Flinn et al., 1992; Murray, 1993, De Boever et al., 1996, De la Roza et al., 1998). The objectives of this study were (1) to determine the potential of NIRS to assess the chemical composition of dried maize samples and (2) to attempt calibrations on undried samples either for farm advisory systems or for animal nutrition research purposes in Uruguay. NIRS were used to assess the chemical composition of whole - plant maize silage samples (Zea mays, L). A representative population of samples (n = 350) covering a wide distribution in chemical characteristics were used. Samples were scanned at 2 nm intervals over the wavelength range 400-2500 nm in a NIRS 6500 (NIRSystems, Silver Spring, MD, USA) in reflectance mode. Cross validation was used to avoid overfitting of the equations. The optimum calibrations were selected on the basis of minimizing the standard error of cross validation (SECV). The calibration statistics were R$^2$ 0. 86 (SECV: 11.4), 0.90 (SECV: 5.7), 0.90 (SECV: 16.9) for dry matter (DM), crude protein (CP), acid detergent fiber (ADF) in g kg$\^$-1/ on dry matter, respectively for maize silage samples. This work demonstrates the potential of NIRS to analyse whole - maize silage in a wide range of chemical characteristics for both advisory farm and nutritive evaluation.