• The Journal of Korean Obstetrics and Gynecology
• /
• v.24 no.3
• /
• pp.28-47
• /
• 2011

Objectives: This study was performed to investigate the effects of Angelicae gigantis Radix (AGR) which is one of the most useful herbal-drug to treat patients with Polycystic Ovary Syndrome (PCOS) in Oriental medicine on gene expression of ovary tissue. Methods: The effects of AGR on gene expression of ovary tissue resected from PCOS induced rats using single injection of $\ss$-Estradiol 17-valerate (EV) was measured using microarray technique, and the functional analysis on these genes was conducted. Results: Total 2,812 genes were up-regulated or down-regulated, 1,421 genes were up-regulated, 1,391 genes were down-regulated by induction of PCOS. Up-regulated genes were mainly involved in biological function such as cell signalling pathways and inflammatory response. Expression levels of 1,442 genes were restored to those of naive animals by administration of AGR. 558 genes were restored to those of naive animals, which were lowered by induction of PCOS. 884 genes were lowered to naive levels, which were elevated by induction of PCOS. The functions of restored genes were partially involved in the restoration of expression levels, which were changed by induction of PCOS. Especially, up-regulated gene by induction of PCOS were mainly involved in these changes. These results mean restorative effects of AGR on damaged functions by induction of PCOS. The network of total protein interactions was measured using cytoscape program, and some key molecules, such as IRS2, MCM10, ORC2L related in up-regulated genes, CTBP2, CD44, RHOA, related in down-regulated genes that can be used for elucidation of therapeutical mechanism of medicine in future were identified. Conclusion: Restored genes by AGR were thought to have common pathways related in regulation of gene expressions. Especially, genes in restored expression levels by AGR, which were up-regulated by induction of PCOS, were regulated by 9 of common transcription factors, genes in restored expression levels by AGR, which were down-regulated by induction of PCOS, were involved in 25 of common transcription factors.

• Archives of Pharmacal Research
• /
• v.21 no.6
• /
• pp.645-650
• /
• 1998

The dual drug-loaded alginate beads simultaneously containing drug in inner and outer layers were prepared by dropping plain (single-layered) alginate beads into $CaCl_2$ solution. The release characteristics were evaluated in simulated gastric fluid for 2 h followed by intestinal fluids thereafter for 12 h. The surface morphology and cross section of dual drug-loaded alginate beads was also investigated using scanning electron microscope (SEM). The poorlv water-soluble ibuprofen was chosen as a model drug. The surface of single-layered and dual drug-loaded alginate beads showed very crude and roughness, showing aggregated particles, surface cracks and rough crystals. The thickness of dual drug-loaded alginate beads surrounded by outer layer was ranged from about 57 to 329mcm. The distinct chasm between inner and outer layers was also observed. In case of single-layered alginate bead, the drug was not released in gastric fluid but was largely released in intestinal fluid. However, the release rate decreased as the reinforcing $Eudragit^{\circledR}$ polymer contents increased. When the plasticizers were added into polymer, the release rate largely decreased. The release rate of dual drug-loaded alginate beads was stable in gastric fluid for 2 h but largely increased when switched in intestinal fluid. The drug linearly released for 4 h followed by another linear release thereafter, showing a distinct biphasic release characteristics. There was a difference in the release profiles between single-layered and dual drug-loaded alginate beads due to their structural shape. However, this biphasic release profiles were modified by varying formulation compositions of inner and outer layer of alginate beads. The release rate of dual drug-loaded alginate beads slightly decreased when the outer layer was reinforced with $Eudragit^{\circledR}$ RS1OO polymers. In case of dual drug-loaded alginate beads with polymer-reinforced outer layer only, the initial amount of druc released was low but the initial release rate (slope) was higher due to more swellable inner cores when compared to polymer-reinforced inner cores. The current dual drug-loaded alginate beads may be used to deliver the drugs in a time dependent manner.

• Journal of Mushroom
• /
• v.2 no.4
• /
• pp.200-206
• /
• 2004

The optimal temperature for mycelial growth the F. fomentarius was $30^{\circ}C$ and the range of the temperature for mycelial growth wsa about 10~30. The optimal pH for the growth was 4.0. The percentage of weight loss percentage wsa 17.4%. The percentage of WEC extractives wsa increased to 2.24%. The observation of micromorphological showed that the detected cell wall were erosive and thinning as typical degradation pattern of white-rot fungi.

• Proceedings of the Korean Vacuum Society Conference
• /
• 2013.08a
• /
• pp.250-250
• /
• 2013

단일벽 탄소나노튜브(Single-walled nanotubes, SWNTs)는 나노스케일의 크기와 우수한 물성으로 인하여, 전자, 에너지, 바이오 분야로의 응용이 기대되고 있다. 특히 SWNTs의 직경을 제어하게 되면 튜브의 전도성 제어가 훨씬 수월하게 되어, 차세대 나노전자소자의 실현을 앞당길 수 있으며 이러한 이유로 많은 연구들이 현재 행해지고 있다. SWNTs의 직경제어 합성을 위해서는 현재 열화학기상증착법(Thermal chemical vapor deposition; TCVD)이 가장 일반적으로 이용되고 있으며, 합성 촉매와 합성되는 튜브의 직경과의 크기 연관성이 알려진 후로는, 촉매의 크기를 제어하여 SWNTs의 직경을 제어하고자 하는 연구들이 활발하게 보고되고 있다. 특히, 촉매 나노입자의 직경이 1~2 nm 이하로 감소될 경우, SWNTs의 직경 분포가 어떻게 변화할 것인지가 최근 가장 중요한 관심사로 남아 있으나, 이러한 크기의 금속입자는 나노입자의 융점저하 현상이 발현되는 영역이므로, SWNTs의 합성온도 영역에서 촉매 금속입자는 반액체(Semi-liquid) 상태로 존재할 것으로 추측하고 있다. 본 연구에서는 고온의 SWNTs 합성환경에서 금속나노촉매의 유동성을 제한하기 위하여 나노사이즈의 기공이 규칙적으로 정렬된 다공성 물질인 제올라이트를 촉매담지체로 이용하였고, 이 때 다양한 합성변수가 SWNTs의 직경에 미치는 영향을 살펴보고자 하였다. SWNTs의 합성을 위해 실리콘 산화막 기판 위에 제올라이트를 도포한 후, 합성 촉매로서 전자빔증발법을 통하여 수 ${\AA}$에서 수 nm 두께의 철 박막을 증착하였다. 합성은 메탄을 원료가스로 하여 TCVD법으로 실시하였다. 주요변수로는 제올라이트 종류, 증착하는 철 박막의 두께, 합성온도를 설정하였으며, 이에 따라 합성된 SWNTs의 합성수율 및 직경분포의 변화를 체계적으로 살펴보았다. SWNTs의 전체적인 합성수율의 변화는 SEM 관찰결과를 이용하였으며, SWNTs의 직경은 AFM 관찰 및 Raman 스펙트럼의 분석에서 도출하였다. 실험결과, 제올라이트 종류에 따라서는 명확한 튜브직경 분포의 변화 없이 비교적 좁은 직경분포를 갖는 SWNTs가 합성되었으며, 합성온도가 $850^{\circ}C$ 이하로 감소되면 합성수율이 현저히 감소되는 것을 알 수 있었다. 촉매박막의 두께가 1 nm 이상인 경우에서는 직경 5 nm 전후의 나노입자가 형성되었으며, 이때 SWNTs의 합성수율은 높았으나 다양한 직경의 튜브가 합성이 된 것을 확인할 수 있었다. 반면, 촉매입자의 크기가 2 nm 이하에서는 합성수율은 다소 저하되었으나, SWNTs의 직경분포의 폭이 상대적으로 훨씬 좁아지는 것을 알 수 있었다. 추후, 극미세 촉매와 저온합성 환경에서의 합성수율 향상을 위한 합성공정의 개량이 지속적으로 요구된다.

• The Korean Journal of Mycology
• /
• v.14 no.3
• /
• pp.215-223
• /
• 1986

Protoplasts of P. cornucopiae were reverted to normal hyphal growth and reversion frequency was $0.04{\sim}19%$. The complete medium stabilized with 0.6 M sucrose was most effective for regeneration of protoplasts. When hypertonic mushroom complete medium not containing agar was overlaid, regeneration frequency of protoplasts was the highest rate among the others of topagar. The protoplast reversion frequency and mycelial growth of P. cornucopiae were increased when various amino acids, nucleic acid components and vitamin compound were added to the hypertonic minimal medium. The relation between sources increasing reversion frequency and sources accelerating mycelial growth was similar in amino acids and nucleic acid components but it was different in vitamins. The protoplast reversion frequency showed the highest rate when all sources were added to the regeneration minimal medium. Microscopically, regeneration patterns of protoplasts showed formation of a bud-like structure, direct germination, yeast-like cell chain of the protoplast, and the production of both direct germ tube and yeast-like cell chain from a protoplast.

• Journal of Korea Multimedia Society
• /
• v.6 no.2
• /
• pp.340-351
• /
• 2003

We introduce our experiences of the design and implementation of the Page Together system that has expanded hyperlink metaphor to utilize human resources in the web. This system supports that a user connects with others in the web, communicates through video/audio channel, navigates same web pages simultaneously and cooperates some work on Internet. For these functions, it comprises Collaborative Browsing Module (CBM), Multimedia Conferencing Module(MCM) Data Conferencing Module(I)CM) and Multi Protocol Interface(MPI). We adopted three standard protocols, IEC, H.323 and T.120 for each nodule and it allows developers to use them easily. We also defined MPI to synchronize information of session among modules. Each module exchanges information each other in session creating process and session terminating process. After a session is created once, each module works independently as its won protocol. Interferences among modules are reduced as minimizing to exchange information. We also introduce a web site that provides web board service based on the Page Together system. A user may post a notice with a link to himself/herself on our web board. After then, if someone read that notice and has any question about it, he or she can try to connect to the writer as clicking the link in that notice and communicate each other. This service site shows that our system can be applied to diverse internet services such as distance teaming and distance conference.

• Korean Journal of Food Science and Technology
• /
• v.30 no.2
• /
• pp.385-390
• /
• 1998

The effect of agglomeration treatment was examined to prevent the parched cereal powder from clumping when it is blended with water. Parched cereal powder was composed of 66.9% carbohydrate, 7% water, 12.1% crude protein, 12.1% crude fat and 1.9% ash, respectively. Particle size of parched cereal powder was generally enlarged by agglomeration treatment. This phenomenon was confirmed by particle size analyzer and microscopic observation. The color of agglomerated sample was shown to be slightly darker than the untreated sample. The water absorption indices of agglomerated samples which were steamed for 2min and re-dried were significantly increased as compared with the untreated sample. The water solubility indices of agglomerated samples showed generally lower values than those of untreated samples. In views of quality and processing time, the optimum condition of agglomeration treatment for manufacturing well-dispersable parched cereal powder in water was 15min re-drying after 2min steaming. It is concluded that the agglomeration treatment improves the dispersibility of parched cereal powder and thus facilitates the intake of it after mixing with water.

• Korean Journal of Food Science and Technology
• /
• v.30 no.2
• /
• pp.348-355
• /
• 1998

The developments of various processed foods and the high quality dried fruits, in particular, are urgently needed for the enhancement of fruit consumption and their competitive values. Therefore, in this study, three variables by three level factorial design and response surface methodology were used to determine optimum conditions for osmotic dehydration of kiwifruit. The relationships of moisture losses, solid gains, weight reductions, sugar contents, titratable acidities and vitamin C contents depending on changes with temperature, sugar concentration and immersion time were investigated. The moisture loss, solid gain, weight reduction and reduction of moisture content after osmotic dehydration were increased as temperature, sugar concentration and immersion time increased. The effect of concentration was more significant than those of temperature and time on mass transfer. Sugar content was increased by increasing sugar concentration, temperature, immersion time during osmotic dehydration. Titratable acidity and vitamin C content were increased by decreasing temperature, immersion time and increasing concentration during osmotic dehydration. The regression models showed a significant lack of fit (P>0.05) and were highly significant with satisfying values of $R^2$. At the given conditions such as $66{\sim}69%$ moisture content, above $24^{\circ}Brix$ sugar content and more than 23 mg% vitamin C, the optimum condition for osmotic dehydration was $37^{\circ}C,\;55^{\circ}Brix$ and 1.5 hour.

• Journal of Mushroom
• /
• v.14 no.4
• /
• pp.202-206
• /
• 2016

Oyster mushrooms are widely cultivated and consumed in Korea. P. ostreatus 'Suhan(ASI 2504)' is an ideal cultivar for mushroom farmers due to its dark pileus and thick stipe; however, as it is very sensitive to environmental conditions, an alternative cultivar is required. To develop a new cultivar, parental strains 'Suhan(ASI 2504)' and 'ASI 0665 (Heuktari)' were selected from various collected strains according to morphological characteristics. P. ostreatus 'Soltari' was developed by DiMon crossing between the dikaryotic strain 'Suhan' and the monokaryotic strain derived from 'Heuktari'. Thirty-eight of the 100 crossed strains were selected following analysis of mitochondrial genetic characteristics, and 'Soltari' was ultimately selected by continuous cultivation tests. The mitochondrial DNA profile of 'Soltari' was found to be the same as that of 'Heuktari, and a nuclear DNA profile of 'Soltari' was similar as those of the parental strains, 'Suhan' and 'Heuktari.' 'Soltari' mycelium grows adequately in moderate to high temperatures of $12-20^{\circ}C$, although its optimum temperature was found to be $30^{\circ}C$. Fruiting body production per 1.1-L cultivation bottle was approximately 158.8 g. Its stipe length and thickness were comparable to those of diameter and thickness were somewhat lower (42.72 vs. 51.33 mm and 18.18 vs. 22.46 mm, respectively). 'Soltari' was found to be more resistant to high $CO_2$ atmosphere than 'Suhan', and the color of the pileus of 'Soltari' was dark gray at high temperature. Therefore, it is suggested that this new cultivar 'Soltari' is a good alternative cultivar and will contribute to energy saving in oyster mushroom farms.