• BMB Reports
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• v.48 no.10
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• pp.571-576
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• 2015

SB743921 is a potent inhibitor of the spindle protein kinesin and is being investigated in ongoing clinical trials for the treatment of myeloma. However, little is known about the molecular events underlying the induction of cell death in multiple myeloma (MM) by SB743921, alone or in combination treatment. Here, we report that SB743921 induces mitochondria-mediated cell death via inhibition of the $NF-{\kappa}B$ signaling pathway, but does not cause cell cycle arrest in KMS20 MM cells. SB743921-mediated inhibition of the $NF-{\kappa}B$ pathway results in reduced expression of SOD2 and Mcl-1, leading to mitochondrial dysfunction. We also found that combination treatment with SB743921 and bortezomib induces death in bortezomib-resistant KMS20 cells. Altogether, these data suggest that treatment with SB743921 alone or in combination with bortezomib offers excellent translational potential and promises to be a novel MM therapy.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1991.04a
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• pp.356-362
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• 1991

자동차, 항공기, 선박등과 같은 산업제품과 전화기, TV브라운관과 같은 가전제품, 심미적 기능을 갖는 제품등과 같은 일상용품등은 많은 부분이 자유곡면(sculptured surface)으로 이루어져 있다. 이러한 해석적으로 정의 하기 어려운 제품 또는 그것을 생산하는데필요한 금형을 가공하는데 있어서 기존에는 석고 모형이나 목형을 이용한 모방 절삭을 하였다. 그러나 근래에는 자유곡면으로이루어진 제품을 설계하고 가공하는데 있어서 CAD/CAM system을 이용하고 있다. 제품의 곡면을 표현하거나 이들을 NC가공하기 위해서는 곡면을 나타내는 형상 정보가 필요 하고 이들 형상정보로 곡면모델링을 하여공구 경로를 구한다. 그러므로 이들 형상정보를 허용한도내에서 적절히 정하여 실제형상에 가까운 곡면을 형성하여야 한다. CAD/CAM 기술의 발달에 따라 다양한 형태의 곡면을 형성하는 기능을 가진 system이 많이 출현되었고 점토, 석고 또는 나무등으로 만든 physical model로 부터 얻어진 형상정보에 의해 surface fitting을 함으로서 자유곡면을 표현하는 방법이 많이 사용되고 있다. 어떠한 곡면을 표현할때는 곡면의 특성을 잘 표현하면서 전체적으로 smooth 한 것이 바람직하다. 그러므로 곡면의 형태를 설계자가 쉽게 이해할 수 있고 적은 수의 patch로도 복잡한 형상을 나타내야 하며 또한 국부적으로 수정이 용이하여야한다. 본 논문에서는 자유곡면을 나타낼 수 있는 수학적 표현 방법에 관하여 논하고, 해석적 곡면으로 부터 형상정보를 얻어 곡면 모델링을 한 후의 Ferguson곡면, UBS와 NUBS의 차이점을 분석하고, 이들 곡면과 해석적 곡면으로부터 얻은 실제곡면과의 오차를 측정하여 실제형상에 가까운 모델링 곡면을 찾고자 하였다.. 라. MCl(M:K$^{+}$, $Na^{+}$, NH$_{4}$$^{+}$ 및 H$^{+}$) 수용액 메디움에서의 Cd(II), Mg(II) 및 Zn(II)의 Dowex 1-X8, Cl$^{-}$ 수지에 대한 흡착은 역시 어떤 메디움에서도 Cd(II) 흡착이 제일 크며, 다음이 Zn(II) 이고 착이온을 형성않는 Mg(II)이 제일 작았다. 한편 메디움 종류별 D값의 크기순위는 H$^{+}$>K$^{+}$> $Na^{+}$>NH$_{4}$$^{+}$이였다. 메디움의 종류에 따라 D값의 차이가 나는 것은 금속이온의 착이온 형성과 금속이온의 용액내에서의 이온종의 상태와 관련이 있다고 생각된다. 마. MCl(M:K$^{+}$, $Na^{+}$, NH$_{4}$$^{+}$ 및 H$^{+}$)과 MNO$_{3}$ 용리액에 의한 Cd(II), Mg(II) 및 Zn(II)의 용리는 예상한 바와 같이 MCl에서 작은 Dv 값을 갖는데, 이것은 CdCl$_{4}$$^{2-}$ 착이온을 형성하거나 ZnCl$_{4}$$^{2-}$ , ZnCl$_{3}$$^{-}$같은 이온과 MgCl$^{+}$, MgCl$_{2}$같은 이온종을 형성하기 때문인것 같다. 한편 어떠한 용리액에서던지 N

• Analytical Science and Technology
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• v.16 no.1
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• pp.39-47
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• 2003

The measurement of radium ($^{226}Ra$) in the groundwater was established using ${\gamma}$-ray spectroscopy without sample preparation. The background interference by air borne radon daughter nuclides was reduced by $N_2$ gas flow into the counting chamber. Leakage of radon gas produced in the radioactive equilibrium with radium and its daughter nuclides was prevented by use of the air-tighted aluminium container. We investigated the effect of air layer in the counting container. Radioactivity variation due to emanation of radon into the air layer was within the counting error range 5%. When the nitrogen gas was flowed around the detector, peak counts of ${\gamma}$-rays from the daughters of airborne radon was decreased and detection limit was decreased to 0.02 Bq/L. The detection limit of detector was lower than 0.74 Bq/L, the $^{226}Ra$ Maximum Contaminant Level (MCL) in the groundwater proposed by US Environmental Protection Agency (EPA). It was confirmed that $^{226}Ra$ radioactivity in the groundwater could be determined by the ${\gamma}$-ray spectroscopy.

• Journal of Microbiology and Biotechnology
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• v.15 no.6
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• pp.1330-1336
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• 2005

Polyhydroxyalkanoic acid (PHA) inclusion bodies were analyzed in situ by $^{13}C$-nuclear magnetic resonance ($^{13}C$-NMR) spectroscopy. The PHA inclusion bodies studied were composed of poly(3-hydroxybutyrate) or poly(3hydroxybutyrate-co-4-hydroxybutyrate), which was accumulated in Hydrogenophaga pseudoflava, and medium-chain-length PHA (MCL-PHA), which was accumulated in Pseudomonas fluorescens BM07 from octanoic acid or 11-phenoxyundecanoic acid (11-POU). The quantification of the $^{13}C$-NMR signals was conducted against a standard compound, sodium 2,2-dimethyl-2-silapentane-5-sulfonate (DSS). The chemical shift values for the in vivo NMR spectral peaks agreed well with those for the corresponding purified PHA polymers. The intracellular degradation of the PHA inclusions by intracellular PHA depolymerase(s) was monitored by in vivo NMR spectroscopy and analyzed in terms of first-order reaction kinetics. The H. pseudoflava cells were washed for the degradation experiment, transferred to a degradation medium without a carbon source, but containing 1.0 g/l ammonium sulfate, and cultivated at $35^{\circ}C$ for 72 h. The in vivo NMR spectra were obtained at $70^{\circ}C$ for the short-chain-length PHA cells whereas the spectra for the aliphatic and aromatic MCL-PHA cells were obtained at $50^{\circ}C\;and\;80^{\circ}C$, respectively. For the H. pseudoflava cells, the in vivo NMR kinetics analysis of the PHA degradation resulted in a first-order degradation rate constant of 0.075/h ($r^{2}$=0.94) for the initial 24 h of degradation, which was close to the 0.050/h determined when using a gas chromatographic analysis of chloroform extracts of sulfuric acid/methanol reaction mixtures of dried whole cells. Accordingly, it is suggested that in vivo $^{13}C$-NMR spectroscopy is an important tool for studying intracellular PHA degradation in terms of kinetics.

• Journal of Microbiology and Biotechnology
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• v.29 no.1
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• pp.79-90
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• 2019

Lichens are generally known as self-sufficient, symbiotic life-forms between fungi and algae/cyanobacteria, and they also provide shelter for a wide range of beneficial bacteria. Currently, bacterial-derived biodegradable polyhydroxyalkanoate (PHA) is grabbing the attention of many researchers as a promising alternative to non-degradable plastics. This study was conducted to develop a new method of PHA production using unexplored lichen-associated bacteria, which can simultaneously degrade two ubiquitous industrial toxins, anthracene and naphthalene. Here, 49 lichen-associated bacteria were isolated and tested for PHA synthesis. During the GC-MS analysis, a potential strain of EL19 was found to be a 3-hydroxyhexanoate (3-HHx) accumulator and identified as Pseudomonas sp. based on the 16S rRNA sequencing. GC analysis revealed that EL19 was capable of accumulating 30.62% and 19.63% of 3-HHx from naphthalene and anthracene, respectively, resulting in significant degradation of 98% and 96% of naphthalene and anthracene, respectively, within seven days. Moreover, the highly expressed phaC gene verified the genetic basis of $PHA_{mcl}$ production under nitrogen starvation conditions. Thus, this study strongly supports the hypothesis that lichen-associated bacteria can detoxify naphthalene and anthracene, store energy for extreme conditions, and probably help the associated lichen to live in extreme conditions. So far, this is the first investigation of lichen-associated bacteria that might utilize harmful toxins as feasible supplements and convert anthracene and naphthalene into eco-friendly 3-HHx. Implementation of the developed method would reduce the production cost of $PHA_{mcl}$ while removing harmful waste products from the environment.

• Economic and Environmental Geology
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• v.51 no.1
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• pp.27-38
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• 2018

Groundwaters in different rock types (Mesozoic granite, Precambrian gneiss, and schist) of Ganghwa island, Incheon City were characterized by using naturally occurring radioactive materials (NORM) and hydrogeochemical constituents. For the study, groundwater samples from 69 wells had been collected over eight years. Statistical methods were applied to relate hydrogeochemical components and NORM in the groundwater samples. The groundwater samples belonged to $Ca(Na)-HCO_3$ types. The uranium concentrations in three groundwater samples exceeded 30 ug/L of United States Environmental Protection Agency (US EPA) maximum contaminant level (MCL). The radon concentrations in 28 groundwater samples exceeded 4,000 pCi/L (picocuries per Liter) of US EPA alternative maximum contaminant level (AMCL). Gross-alpha in all the groundwater samples did not exceed 15 pCi/L of US EPA MCL. The average concentrations of uranium and radon in groundwater were the highest in granite area, and then gneiss, schist areas in order. In schist area, the correlation coefficient (R) between radon and $HCO_3$ is -0.40 and R between uranium and $SO_4$ is 0.54. In gneiss area, the R between radon and uranium is 0.55 and the R between uranium and $SO_4$ is 0.41. According to factor analysis, each geological area shows different chemical characteristics. The statistical analysis of whole groundwater resulted in nearly no significant relationship among uranium, radon and chemical constituents. Subsequently, more detailed studies on hydrogeological, geochemical, and geological characteristics related to NORM are required to better understand the behavior and fate of NORM.

• Bulletin of the Korean Chemical Society
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• v.18 no.1
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• pp.27-32
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• 1997

The geometries and energies of the isomers in alkyne complexes MCl(PH3)2(η2-C2H2), M=Rh and Ir, are theoretically investigated using ab initio methods at the Hartree-Fock and up to MP4 level of theory and relativistic effective core potentials for Rh and Ir metals. The optimized structures of Rh complexes, 1-3 at MP2/ECP1 level are in good agreement with the related experimental data. The binding energies of C2H2 to d8-metal fragments are computed to be ∼55 kcal/mol. The vinylidene complexes for Rh and Ir metals are calculated to be much lower in energy than the alkyne complexes. The alkyne-vinylidene rearrangement is possible to proceed exothermically through the intermediate hydrido-alkynyl complexes, 2 or 9. Detailed comparison is given about the geometries and relative energies on Rh and Ir isomers at the various level ab initio calculations with orbital analysis.

• Journal of Life Science
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• v.22 no.10
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• pp.1277-1285
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• 2012

The tumor necrosis, factor-related, apoptosis-inducing ligand (TRAIL) is regarded as a potentially useful anticancer agent with excellent selectivity for cancer cells. However, a considerable number of cancer cells are resistant to apoptosis induction by TRAIL. Developing strategies to overcome this resistance are important for the successful use of TRAIL for cancer therapy. Here, we revealed that siRNA-mediated downregulation of SIRT1 or SIRT1 inhibitor Amurensin G upregulated DR5 and c-Myc and downregulated c-$FLIP_{L/S}$ and Mcl-1, which was associated with sensitization of TRAIL-resistant MCF-7 cells to TRAIL. This result was followed by the activation of caspases, PARP cleavage, and downregulation of Bcl-2 in both TRAIL-treated MCF-7 cells transfected with SIRT1 siRNA and cells co-treated with Amurensin G and TRAIL. Our results suggest that the induction of DR5 and downregulation of c-FLIP via suppression of SIRT1 expression may be a useful strategy to increase the susceptibility of TRAIL-resistant cancer cells to TRAIL-induced cell death.

• Analytical Science and Technology
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• v.12 no.6
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• pp.583-586
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• 1999

The quantitative analysis of nitrate and nitrite in rain, milk and infant formula was done by Ion Chromatography. The nitrite was not detected(<0.1 mg/L) in all the samples. However, the nitrate was detected in the range of 0.1~4.9 mg/L in rain, 9.8~19.8 mg/L in milk, and 80~300 mg/L in infant formula, respectively. Some content of nitrate is close to the maximum contaminant level(MCL) which is 10 mg/L as $NO_3-N$, 44.3 mg/L as $NO_3$.

• Bulletin of the Korean Chemical Society
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• v.18 no.12
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• pp.1264-1268
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• 1997

The poly(methylsilene) (1) was modified with the group 4 metallocene Cp2MCl2/Red-Al (M = Ti, Zr, Hf) combination catalyst and with the group 6 metal carbonyl M(CO)6 (M = Cr, Mo, W) catalyst, producing the highly cross-linked isoluble polymer and the lowly cross-linked soluble polymer, respectively. An interrelationship between molecular weight and percent ceramic residue yield with metal within the respective group was not found. The polymers modified with the group 4 metallocene combination catalysts have higher molecular weight and lower percent ceramic residue yield than the polymers modified with the group 6 metal carbonyl catalysts do. The catalytic activity of group 4 metallocene combinations appears to be higher at ∼100 ℃, but to be lower at very high temperature than those of group 6 metal carbonyls. The pyrolysis of the modified 1 yielded SiC ceramic.