• Laboraroty Animal Research
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• 제34권4호
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• pp.176-184
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• 2018

In this study, we observed chronological changes in the immunoreactivity and expression level of myelin basic protein (MBP), one of the most abundant proteins in the central nervous system, in the hippocampus of Zucker diabetic fatty (ZDF) rats and their control littermates (Zucker lean control; ZLC). In the ZLC group, body weight steadily increased with age; the body weight of the ZDF group, however, peaked at 30 weeks of age, and subsequently decreased. Based on the changes of body weight, animals were divided into the following six groups: early (12-week), middle (30-week), and chronic (52-week) diabetic groups and their controls. MBP immunoreactivity was found in the alveus, strata pyramidale, and lacunosum-moleculare of the CA1 region, strata pyramidale and radiatum of the CA3 region, and subgranular zone, polymorphic layer, and molecular layer of the dentate gyrus. MBP immunoreactivity was lowest in the hippocampus of 12-week-old rats in the ZLC group, and highest in 12-week-old rats in the ZDF group. Diabetes increased MBP levels in the 12-week-old group, while MBP immunoreactivity decreased in the 30-week-old group. In the 52-week-old ZLC and ZDF groups, MBP immunoreactivity was detected in the hippocampus, similar to the 30-week-old ZDF group. Western blot results corroborated with immunohistochemical results. These results suggested that changes in the immunoreactivity and expression of MBP in the hippocampus might be a compensatory response to aging, while the sustained levels of MBP in diabetic animals could be attributed to a loss of compensatory responses in oligodendrocytes.

• 전자통신동향분석
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• 제24권3호
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• pp.24-31
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• 2009

IMT-Advanced 무선전송 시스템 단말모뎀 플랫폼은 다중 반송파 변조 기술, 채널 부호화 기술, 셀 탐색/동기 기술 등 핵심이 되는 요소 기술인 고속 무선 전송 기술을 구현할 수 있는 하드웨어 구조, 기능 및 인터페이스를 설계 제작하였다. 상기 단말모뎀 플랫폼에서는 기저대역 모뎀 물리계층 기능인 변조, 복조, 부호, 복호, 동기를 위한 각각의 FPGA가 실장되는 Daughter Board 형태로 구성되어 L1 기저대역 모뎀 장치에 실장된다. 그리고 PHY 계층(L1)부터 MAE 계층(L2), RRC 계층(L3)까지의 하드웨어 및 소프트웨어 수행을 지원한다. 4G용 단말모뎀을 개발하기 위하여 상용화 이전에 LTE-Advanced 테스트 베드용 단말모뎀 플랫폼을 개발하여 20 MHz 대역폭을 적용 3 km/h의 저속 이동속도에서는 최대 110 Mbps를 수신하고, 최대 55 Mbps를 송신한다. 그리고 120 km/h의 고속 이동속도에서는 최대 55 Mbps를 수신하고, 최대 28 Mbps를 송신한다. 상기 성능을 만족하는 단말모뎀 플랫폼이 개발되면 IMT-Advanced 단말모뎀 플랫폼 기술을 확보하게 된다. 따라서 이동통신 분야에서 기술적인 우위와 시장 선점을 위하여 요소기술 IPR을 확보하고, IMT-Advanced의 표준화 과정에서 이를 국제 표준으로 반영하여 로열티 창출 효과 및 기술 경쟁력을 확보하게 될 것이다. 아울러, LTE 사용자들은 대용량의 고속, 멀티미디어 송 수신을 가능하게 하는 기술로 2010년 이후 가상 현실 서비스, 3D 게임, 센싱 등 사물과 사물이 통신하는 유비쿼터스 서비스로 발전할 것으로 전망한다.

• 한국물환경학회지
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• 제33권3호
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• pp.273-281
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• 2017

The objective of this study is to evaluate the performance of stream purification equipment system combined MBP (Micro Bubble Process) and AGBP (Aerobic Granular Biofilm Process). Based on results, the optimal HRT (Hydraulic Retention Time) of MBP and AGBP set as 0.72 and 2.4 h, respectively. In the results of continuous operation on combined MBP and AGBP, it is possible to achieve a BOD, TSS and T-P removal efficiency of 85, 90 and 94%, respectively. It also confirmed that the water quality of the stream can be purified with increasing circulation flow through water simulation evaluation applied the QUAL-NIER model. Consequently this purification system can resolve the stream purification and dry stream problem.

• Biotechnology and Bioprocess Engineering:BBE
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• 제8권2호
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• pp.89-93
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• 2003

human renin binding protein (hRnBp), showing N-acetylglucosamine-2-epimerase activity, was over-expressed in E. coli, but was mainly present as an inclusion body. To improve its solubility and activity, ubiquitin (Ub), thioredoxin (Trx), maltose binding protein (MBP) and NusA, were used as fusion partners. The comparative solubilities of the fusion proteins were, from most to least soluble: NusA, MBP, Trx, Ub. Only the MBP fusion did not significantly reduce the activity of hRnBp, but enhanced the stability. The Origami (DE3), permitting a more oxidative environment for the cytoplasm in E. coli; helped to increase its functional activity.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 1998년도 추계종합학술대회
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• pp.299-304
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• 1998

초고속 정보통신망에서 ATM 사용자와 ATM 망간의 접속을 위한 ATM 정합장치는 ATM 통신 방식으로 ATM 교환기와 정합하는 각종 정합장치로 구성된다. ATM 정합장치는 155.520 Mbps의 선로 속도를 갖는 기본속도(STM-1 : Synchronous Transport Module-1) 정합장치와 44.736 Mbps의 선로 속도를 갖는 중속(DS-3 : Digital Signal-level 3) 정합장치, 그리고 2.048 Mbps 혹은 1.544 Mbps의 선로 속도를 갖는 저속(DS-l/E) 정합장치, 622.08 Ups의 선로 속도를 갖는 고속(STM4) 정합 장치로 구성된다. 본 논문에서는 ATM 저속, 중속, 기본속도 및 고속 정합장치의 기능 및 구현에 대하여 기술하였다.

• 생명과학회지
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• 제8권1호
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• pp.109-117
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• 1998

The synthesis and methylation in vivo of myleline basic protein(MBP) during the mouse brain devlopment was found to be the highest in youngest brain and declined progressively in mature brains. The relative rate of protein synthesis and methylation was a maximal ration in the youngest brain, This high ratio was wdll correlated with the higher protein methylase I (PM I) activity in younger brains. The jimpy mouse is the most severely affected dysmyelinating mutant and is characterized by failure to incorporate MBP into myelin. sheath. The MBP-specific PM I activity in 15-, 18-, and 21-days old hemizygous jimpy mice(jp/y)brains decreased by 20, 50 and 75%, respectively. Myelin fraction with different degrees of compaction were isolated from bovine brain, the most compact myelin fraction exhibited higher methylaccepting activity than the less compact dense fractions.

• Bulletin of the Korean Chemical Society
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• 제29권4호
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• pp.736-740
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• 2008

The interaction of myelin basic protein (MBP) from bovine central nervous system with divalent calcium ion was studied by isothermal titration calorimetry at 27 ${^{\circ}C}$ in aqueous solution. The extended solvation model was used to reproduce the enthalpies of $Co^{2+}$-MBP interaction over the whole $Co^{2+}$ concentrations. The solvation parameters recovered from the solvation model were attributed to the structural change of MBP due to the metal ion interaction. It was found that there is a set of three identical and noninteracting binding sites for $Co^{2+}$ ions. The association equilibrium constant is 0.015 ${\mu}M^{-1}$. The molar enthalpy of binding is $\Delta$H = −14.60 kJ $mol^{-1}$.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2002년도 Molecular and Cellular Response to Toxic Substances
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• pp.186-186
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• 2002

Di-n-butyl phthalate(DBP) is one of the most commonly used phthalic acid esters(PAEs) as a plasticizer, a solvent or a component in cosmetic formulations. MBP(monobutyl phthalate) is a major metabolite of DBP. Although DBP has been identified as a reproductive toxicant in several animal species and also known as a endocrine disruptor, the direct or indirect immunomodulatory effect of DBP has been little reported.(omitted)

• Archives of Pharmacal Research
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• 제22권3호
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• pp.274-278
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• 1999

Expression of the active urease of the enterobacterium, Klebsiella aerogens, requires the presence of the accessory genes (ureD, ureE, ureF, and ureG) in addition to the three structural genes (ureA, ureB, and ureC). These accessory genes are involved in functional assembly of the nickel-metallocenter for the enzyme. Characterization of ureF gene has been hindered, however, since the UreF protein is produced in only minute amount compared to other urease gene products. In order to overexpress the ureF gene, a recombinant pMAL-UreF plasmid was constructed from which the UreF was produced as a fusion with maltose-binding protein. The MBP-UreF fusion protein was purified by using an amylose-affinity column chromatography followed by an anion exchange column chromatography. Polyclonal antibodies raised against the fusion protein were purified and shown to specifically recognize both MBP and UreF peptides. The UreF protein was shown to be unstable when separated from MBP by digestion with factor Xa.

• The Korean Journal of Ecology
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• 제24권1호
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• pp.35-43
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• 2001

카드뮴과 아연에 각각 그리고 동시에 노출한 총알고둥(Littorina brevicula)에서 중금속의 생체내 축적, 제거 및 세포내 분포 양상을 조사하였다. 총알고둥을 카드뮴 400 $\mu\textrm{g}$/L 또는 아연 3000 $\mu\textrm{g}$/L에 각각 90일간 노출하였을 경우, 각 중금속의 축적량은 노출기간에 따라 증가하였으며, 70일 이후에는 더 이상 축적량이 증가하지 않았다. 카드뮴과 아연을 동시에 노출하였을 경우에는 각각의 중금속에 노출하였을 경우에 비해 아연의 축적량은 증가하였으나, 카드뮴의 축적량은 감소하였다. 노출실험에 이어 수행한 42일간의 청장실험 결과, 카드뮴은 체외로 제거되지 않았으나, 아연은 제거되었다. 특히 카드뮴과 아연에 동시 노출한 총알고둥의 경우에 아연은 더 신속히 제거되었다. 총알고둥을 카드뮴과 아연에 각각 70일 동안 노출한 후, 체내로 흡수된 카드뮴의 약 60%가 soluble fraction에 분포하고 있었으며, 아연의 75%는 insoluble fraction에 분포하고 있었다. 이러한 경향은 카드뮴과 아연의 동시 노출시에도 유사하게 나타났다. 카드뮴과 아연은 soluble part내의 리간드(ligand) 와의 결합 양상에서도 차이를 나타냈는데, 카드뮴은 90% 이상이 약 6.5 kDa크기의 MBP-1(Metal-Binding Protein-1)과 결합하고 있었으나, 아연은 HMW(High molecular weight fraction, >60 kDa), MBP-1, MBP-2, LMW(Low molecular weight fraction <1 kDa)에 고루 분포하는 것으로 나타났다.