• Asian Pacific Journal of Cancer Prevention
• /
• v.14 no.11
• /
• pp.6433-6438
• /
• 2013

In hepatocellular cancer (HCC), lack of response to chemotherapy and radiation treatment can be caused by a loss of epigenetic modifications of cancer cells. Methionine adenosyltransferase 1A is inactivated in HCC and may be stimulated by an epigenetic change involving promoter hypermethylation. Therefore, drugs releasing epigenetic repression have been proposed to reverse this process. We studied the effect of the demethylating reagent 5-aza-2'-deoxycitidine (5-Aza-CdR) on MAT1A gene expression, DNA methylation and S-adenosylmethionine (SAMe) production in the HCC cell line Huh7. We found that MAT1A mRNA and protein expression were activated in Huh7 cells with the treatment of 5-Aza-CdR; the status of promoter hypermethylation was reversed. At the same time, MAT2A mRNA and protein expression was significantly reduced in Huh7 cells treated with 5-Aza-CdR, while SAMe production was significantly induced. However, 5-Aza-CdR showed no effects on MAT2A methylation. Furthermore, 5-Aza-CdR inhibited the growth of Huh7 cells and induced apoptosis and through down-regulation of Bcl-2, up-regulation of Bax and caspase-3. Our observations suggest that 5-Aza-CdR exerts its anti-tumor effects in Huh7 cells through an epigenetic change involving increased expression of the methionine adenosyltransferase 1A gene and induction of S-adenosylmethionine production.

• Geotechnical Engineering
• /
• v.6 no.3
• /
• pp.53-64
• /
• 1990

Load tests for ten small-scale foundation models combined with geotextile and sand mat were conducted to study the effect of geotextile, sand mat, and foundation types on deformation of foundation soils. In addition, the experimental results were compared with those obtained from numerical analysis using a software program. The main conclusions were summarized as follows : 1. The restraint effect on GIT is more outstanding on the lateral displacement than on the vertical one. 2. The only use of SIM has better effect for the restraint of lateral displacement than vertical ogle. 3. The use of both SIM and GIT are required for the restraint of lateral and vertical displacement.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.41 no.2
• /
• pp.51-55
• /
• 2020

The objective of this study was to evaluate the changes in gene expression after incubation of cells with soluble fraction from different silk mat layers. A silk cocoon from Bombyx mori was separated into 4 layers of equal thickness. The layers were numbered from 1 to 4 (from the inner to outer layer). Each silk mat was placed into normal saline and collected soluble fraction. They were administered to RAW264.7 cells, and changes in the expression of genes were evaluated by cDNA microarray analysis. Layer 1 and 4 groups showed significantly higher expression of BMP-2 at 8 h after administration of soluble fraction (P < 0.05). Runx2 expression was significantly higher in Layer 4 group at 8h (P < 0.05). The silk mat from the innermost and outermost portion of the silkworm cocoon showed a significant change in the expression of genes that are associated with osteoinduction such as BMP-2 and runx2.

• Journal of Plant Biotechnology
• /
• v.47 no.1
• /
• pp.15-25
• /
• 2020

Allium L. is one of the largest genera of the Amaryllidaceae family, with more than 920 species including many economically important species used as vegetables, spices, medicines, or ornamental plants. Currently, DNA barcoding tools are being successfully used for the molecular taxonomy of Allium. A total of 46 Allium species were collected from their native areas, and DNA was extracted using the IBRC DNA extraction kit. We used specific primers to PCR amplify matK. DNA sequences were edited and aligned for homology, and a phylogenetic tree was constructed using the neighbor-joining method. The results show thymine (38.5%) was the most frequent and guanine (13.9%) the least frequent nucleotide. The matK regions of the populations were quite highly conserved, and the amount of C and CT was calculated at 0.162 and 0.26, respectively. Analysis of the nucleotide substitution showed C-T (26.22%) and A-G (8.08%) to have the highest and lowest percent, respectively. The natural selection process dN/dS was 1.16, and the naturality test results were -1.5 for Tajima's D and -1.19 for Fu's Fs. The NJ dendrogram generated three distinct clades: the first contained Allium austroiranicum and A. ampeloprasum; the second contained A. iranshahrii, A. bisotunense, and A. cf assadi; and the third contained A. rubellum and other species. In this study, we tested the utility of the matK region as a DNA barcode for discriminating Allium. species.

• Journal of Biosystems Engineering
• /
• v.4 no.1
• /
• pp.48-57
• /
• 1979

In order to obtain a standard reference for designing an adequate power rice transplanter, the cutting forces depending upon variety of seedling, sowing density, seedling age and soil moisture content of mat-type seedling were measured by the rice transplanter installed with force measuring device of dynamic strain gage system in the laboratory. The result of this study are summarized as follows : 1. Cutting velocity and acceleration transplanting hoe obtained from jinematic analysis of planting mechanism was 1.32m/sec and 81.5m/$sec^2$ when planting crank-shaft rpm was 160. 2. Little difference between cutting forces on 30-days old seelings of japonica and Indica type was observed, as the cutting forces determined were 2.0kg per hill for Japonica type and 2.1kg per hill for Indica type. 3. Cutting forces determined on 40-days old seedlings were 2.5kg, 2.3kg, 3.1kg and 2.9kg per hill for Milyang No.15, Tongil, Akibare and Milyang No.23 compared to the other varieties. 4. The cutting force was not greatly affected by the sowing densities , only five percent of differences were observed epending upon the sowing densities. 5. Cutting forces were 2.7kg and 2.0kg per hill on 40-days old seedlings and 30-days old seedlings respectively. About 38 percent of more forces was required in cutting 40-days old seedling than in cutting 30-days old seedlings. 6. More cutting forces were required as soil moisture content of mat-type seedling was decreased. 7. Root length after cutting by the planting hoe and their relationships with soil moisture content on 30-days old seedlings, are as follows ; $y=4.147-11.384x+ 28.854x^2$ where , $y$=root length after cutting. (cm) , $x$=soil ture content of mat type seedlings.(%, d.b.) 8. Cutting forces were varied with the width of cuttings ; those on 40-days old mat type seedlings were 2.7kg and 2.2kg per hill when cutting with 14 mm and 10mm of width respectively, about 32 percent of more forces was required when cuting with 14mm of width compared to 10mm of width.

• Journal of The Korean Society of Agricultural Engineers
• /
• v.53 no.4
• /
• pp.29-37
• /
• 2011

Effect of rice straw mat and wood shaves on the reduction of runoff and nonpoint source (NPS) pollution loads from field plots were experimentally studied. Three runoff plots of $5{\times}22$ m in size and 3 % in slope were prepared on a loamy sand field. Each plot was equipped with a flume to measure runoff and collect water samples. Experimental treatments of surface cover were bare, wood shaves (1,000 kg/ha) and rice straw mat cover (3,000 kg/ha). Under radish was cultivation. During the growing season of the radish, three rainfall-runoff events were monitored. Effect of wood shaves and straw mat cover on runoff reduction was 4~30 % and 33~75 % respectively compared to control. The effect on NPS pollution reduction was 36.8 and 64.3 % in BOD, 41.1 and 80.8 % in SS, 34.0 and 56.1 % in TP and 28.0 and 56.6 % in TN respectively. It was analyzed that the reduction of runoff and NPS pollution were mainly contributed by the decrease of rainfall energy impact and flow velocity and the increase of infiltration due to the surface cover materials. Rice straw mat showed very stable soil cover while large portion of wood shaves were lost during heavy storm events. It was concluded that straw mat was an efficient cover material to reduce NPS pollution from upland fields.

• Journal of the Korean Geotechnical Society
• /
• v.23 no.3
• /
• pp.25-40
• /
• 2007

Excessive earth pressure is one of the major mechanical factors in the deformation and damage of Cut-and-Cover Tunnel lining in shallow tunnels and portals of mountain tunnels (Kim, 2000). Excessive earth pressure may be attributed to insufficient compaction and consolidation of backfill material due to self-weight, precipitation and vibration caused by traffic (Komiya et al., 2000; Taylor et al., 1984; Yoo, 1997). Even though there were a lot of tests performed to determine the earth pressure acting on the tunnel lining, unfortunately there were almost no case histories of studies performed to determine remedial measures that reduce differential settlement and excessive earth pressure. In this study the installation of geotextile mat was selected to reduce the differential settlement and excessive earth pressure acting on the cut-and-cover tunnel lining. In order to determine settlement and earth pressure reduction effect (reinforcement effect) of geotextile mat reinforcement, laboratory tunnel model tests were performed. This study was limited to the modeling of rigid circular cut-and-cover tunnel constructed at a depth of $1.0D\sim1.5D$ in loose sandy ground and subjected to a vibration frequency of 100 Hz. Model tests with varying soil cover, mat reinforcement scheme and slope roughness were performed to determine the most effective mat reinforcement scheme. Slope roughness was adjusted by attaching sandpaper #100, #400 and acetate on the cut slope surface. Mat reinforcement effect of each mat reinforcement scheme were presented by the comparison of earth pressure obtained from the unreinforced and mat reinforced model tests. Soil settlement reduction was analyzed and presented using the Picture Analysis Method (Park, 2003).

• Journal of the Korean Geosynthetics Society
• /
• v.18 no.1
• /
• pp.55-65
• /
• 2019

This paper describes results of experimental and numerical analyses, in order to analyze a reinforcement effect of concrete mat on open cut restoration of underground cavity. The plate loading tests were conducted to evaluate a reinforcement effect of concrete mat, at reinforcement depths from the ground surface of 10 cm, 20 cm, and 30 cm. The result showed that the reduction ratio of stress (earth pressure) was about 60% at all reinforcement depth. The reinforcement effect considering ground surface settlement and reduction ratio of stress based on laboratory tests and numerical analysis was significant, at reinforcement depths from the ground surface of 10 cm~20 cm. LFWD test results showed that subgrade modulus was the largest when concrete mat was installed 20 cm below ground surface. Therefore, it is effective to reinforce concrete mat within 20 cm from the surface, when the underground cavity due to damage of underground utilities was formed in the height direction to the bottom of the pavement layer.

• Journal of the Korean GEO-environmental Society
• /
• v.10 no.6
• /
• pp.5-18
• /
• 2009

In construction of buildings in Korea, the buildings are frequently founded on the weathered ground (weathered soil/rock, fractured rock). In this case, to make a full use of a bearing capacity of a weathered ground for economic design, the shallow mat foundation system could be used. In this study, we have researched three cases of mat foundations on the weathered ground in Korea, and analyzed and considered the design procedures and the reinforcing methods. That is, we have considered the detail design, analysis proceedings, the ground settlement evaluation proceedings, the rock face mapping evaluations after excavation and reinforcing methods of the mat foundation on the weathered ground. And large scale plate load tests on the weathered ground supporting the mat foundation were performed and also load bearing capacity and settlement of actual mat foundation, considering the scale effect, were evaluated.

• Neonatal Medicine
• /
• v.16 no.2
• /
• pp.239-243
• /
• 2009

Multifocal atrial tachycardia (MAT) is a rare arrhythmia in the newborn. MAT can be difficult to diagnose; it is frequently confused with atrial fibrillation. MAT is difficult to treat but often resolves spontaneously within the first year of life. A newborn with a rapid and irregular pulse rate was diagnosed with multifocal atrial tachycardia by eletrocardiography (ECG) using a hand-made transesophageal electrode. Treatment with propranolol was attempted but ineffective. Treatment with digoxin and sotalol was attempted. The heart rhythm gradually reverted to a sinus rhythm with this treatment. We report our experience managing a neonate with MAT diagnosed by ECG using a hand-made transesophageal electrode.