• KSBB Journal
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• v.29 no.1
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• pp.58-66
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• 2014

Demand for in vitro pharmacological evaluation and toxicity test using human hepatocytes has been increasing. In USA and Europe, human hepatocytes obtained from donated whole liver unsuitable for transplantation were distributed to researchers and deposited in cell bank facility as cryopreserved vial. In Korea, however, incidence of transplantation- inappropriate whole liver has been quite low and the whole livers almost have so severe liver disease such as fatty or fibrotic liver that cannot meet the demand. In this study we aimed to isolate human hepatocytes from liver resection surgery-originated partial liver, and assure the isolated human hepatocytes and its cryopreserved hepatocytes to be qualified for the in vitro pharmacological evaluation and drug toxicity tests. We compared those with commercially available human hepatocyte, BD $GenTest^{TM}$ by cell morphology, hepatic gene expression, urea synthesis, albumin secretion, ammonia removal, and cytochrome P450 induction activities. Changes in hepatotoxic gene expression after cryopreservation are evaluated with a typical hepatotoxic drug, acetaminophen. Consequently, the fresh hepatocytes from the partial liver and its cryopreserved hepatocytes expressed their intrinsic hepatic functions well and showed equal hepatotoxicity gene expression trend regardless to cryopreservation. Therefore, liver resection surgery-originated partial liver can be used as a useful source of human hepatocytes for various pharmacological and hepatotoxicity test.

• Journal of Fisheries and Marine Sciences Education
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• v.8 no.1
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• pp.41-47
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• 1996

The fishing experiment was carried out in order to analyses the mesh selectivity for trawl cod - end in the Southern Korean Sea and East China Sea from June,1991 through December, 1992. The mesh selectivity was examined for the five kinds with opening mesh size, of which having mesh s izes 51.2mm, 70.2mm, 77.6mm, 88.0mm and 111.2mm. In this report, mesh selectivity were taken analyses trawl cod - end for Priacanthus macracanthus. The main results obtained were as follows : 1. The relation equations between total length(TL) and body height(BH), body girth(BG) and body weight(BW) were BH=0.256 TL+2.963, BG=0.620 TL+8.650 and $BW=7.34{\times}10^{-5}TL^{2.6956}$, respectively. 2. 50% selection length and selection factor of trial cod - end with opening mesh size having 51.2mm, 70.2mm, 77.6mm, 88.0mm were 94.8mm, 1.85 ; 159mm, 2.27 ; 204mm, 2.09 ; 217.3mm, 2.18, respectively. 3. Range of opening mesh size from 51.2mm to 88.0mm, parameters of master curve were slope 2.70, intercept - 5.7 and selection factor 2.11.

• Journal of Digital Convergence
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• v.12 no.11
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• pp.575-583
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• 2014

The purpose of this study was to investigate factors affecting depression among female marriage immigrants in relation to demographic, family, self-efficacy, acculturation, and physical health aspects. The subjects were 556 marriage immigrants residing in Seoul, Keong-gi, and Chung-cheong areas. 47.1% of the subjects showed $${\geq_-}16$$ of CES-D which is the cutting points on depression. The results from hierachical regression analysis indicated that family income(${\beta}=-.13$, p=.002) at the first demographic model and self-efficacy(${\beta}=-.13$, p<.001), family relationship(${\beta}=-.16$, p<.001), acculturation to Korean culture(${\beta}=-.08$, p=.05), acculturative stress(${\beta}=.31$, p<.001), and mal-physical health(${\beta}=.18$, p<.001) at the final model affected female marriage immigrants' depression. The explanatory power of these variables was 37.1%. Based on these results, social services and policies for the prevention of depression among female marriage immigrants were discussed.

• MALSORI
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• no.21_24
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• pp.43-89
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• 1992

In this thesis, the prominent aspect of word accent in standard Korean is studied by auditory test and acoustic analysis experiment. The definition of 'accent' is, following Hoyoung Lee's discussion(1990), to be described as 'the means whereby a focused part of an utterance is made to stand out in order to concentrate the hearer's attention on it.' That is to say, the ten of 'accent' may be described in terms of phonological phenomenon and the accented syllable can be phonetically prominent as the result of those phonological process. Prosodic features may have different characteristics in different languages whether they contain linguistically important functions or not. Thus the characteristics of word accent in standard Korean will be determined as the content and trait of prosodic features. Following this viewpoint, present study looked over prosodic features which may effect the characteristics of word accent in standard Korean, through systematic experimental procedure. And the result of this experiment has been verified by statistical method, the T-test, for the purpose of identifying the relatedness among prosodic features(parameters). This thesis, therefore, aimed to investigate the intrinsic acoustic and physical qualities of the word accent in standard Korean. Nonsense words composed by 'mal' and 'ma' which can be divided into 'heavy syllable' and 'light syllable' quoted from Hyman(1975) have been classified into 28 types with respect to syllable numbers(2 syl., 3 sy1., 4 syl.) and these words have become the target of auditory test and acoustic experiment. As the result of those experimental Procedures, the word accent in standard Korean may be said that it has a tendency of fixing first two syllables regardless of syllable numbers. The syllable types of HH, HL, LL in the first two syllables may be prominent at first syllable and the type of H may be at second syllable. Various prosodic features(parameters) including duration, intensity, and Fo(purely phonetic terms) were also strengthened in those positions. The result of this experiment can be cleared up like these : 1. The most important feature is proved as 'duration', the feature of intensity resulted in more subsidiary one than the feature of duration. 2. Fo( fundamental frequency) could be observed as having some coherent contour through almost all syllable types(99 ％), that is, in 2 syllable types, it had rising contour, in 2 syllable types, rising-falling contour, and in 4 syllable types, it contained rising-falling-rising contour. The result of auditory test was different with those contour forms of all Fo surveyed. With respect to these results, the discuss for Fo is determined' to be excluded comparing other features. 3. Finally, this thesis resulted in a decision that the word accent in standard Korean may has fixed(somewhat weaker) accent, especially fixed at first two syllables in almost all words. 4. Various kinds of syllable types related with 2,3,4 syllables, therefore, can be reclassified into 4 types of HH, HL, LH, LL following the concept of accent fixing placement(i.e. first two syllables). In these 4 types, the types of HH, HL, LL were prominent at the position of the first syllable , and the type of LH was prominent at the second syllable otherwise.

• The Journal of Korean Physical Therapy
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• v.12 no.2
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• pp.255-265
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• 2000

The term 'myositis ossificans' encompasses four categories of clinicopathological disorders. The first, myositis ossificans progressive(fibrodysplasia ossificans progressive), is a rare genetic disease characterized by progressive heterotopic ossification involving skeletal muscle, tendon, ligaments, and fascia, with congenital malformation of the great toes, and usually microdactyly, monophalangism, and mal formed proximal phalanges. with valgus deformity of metatarsophalangeal joint. The ossification begins shortly after birth and may contribute to the patient's death. The second, heterotopic ossificans, can occur in patients with neuromuscular and chronic diseases such as paraplegia, poliomyelitis, polymyositis, bum, tetanus, and infection. But the lesions in these cases often lack the typical histologic features of myositis ossificans. The third, myositis ossificans traumatica, is the most common; it develops in response to soft tissue trauma such as a single severe injury, minor repetitive injures, fracture, joint dislocation, stab wound, or surgical incision. The forth, nontraumatic myositis ossificans, also designated :pseudomalignant osseous tumors of extraskeletal soft tissues' and 'psedomalignant myositis ossificans', occurs in persons repeated small mechanical injures or nonmechanical soft tissue injuries due to local ischemia, inflammation. or other factors cannot be ruled out in such cases.

• Food Science and Preservation
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• v.15 no.4
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• pp.576-581
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• 2008

The chemical components of the wild vegetables Solidago virgaurea spp. gigantea, Aster glehni var. hondoensis, and Aruncus dioicus var. kamtschaticus grown on Ulleung island, Korea were investigated. In dried powders of these three wild vegetables, the contents of moisture, crude protein, crude lipid, crude fiber, and crude ash were 3.77-5.72%, 15-29%, 3.50-6.68%, 4.00-6.01%, and 8.70-10.54%, respectively. There were differences in the levels and nature of organic acids in the vegetables; the major organic acids were succinic acid, citric acid, and malonic acid. The major free amino acids in the vegetables were glutamic acid, aspartic acid, arginine, and phenylalanine. Riboflavin contents were 90 mg% in Aruncus dioicus var. kamtschaticus and $2{\sim}3$ mg% in the other vegetables. The thiamin contents were 113 mg% in Aster glehni var. hondoensis, 85 mg% in Aruncus dioicus var. kamtschaticus, and 71 mg% in Solidago virgaurea spp. gigantea. The major fatty acids in the three vegetables were linolenic acid, linoleic acid, and palmitic acid; 60% of total fatty acid was linolenic acid. Catechins were present at 5.37 mg% in Aruncus dioicus var. kamtschaticus, 2.46 mg% in Solidago virgaurea spp. gigantea, 1.29 mg% in Aster glehni var. hondoensis.(-) ECC was detected in only Aruncus dioicus var. kamtschaticus. The contents of Ca, P, and Na in the three vegetables were higher than the contents of other minerals.

• The Korean Journal of Mycology
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• v.24 no.2 s.77
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• pp.93-103
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• 1996

In order to find out effect on the inorganic and organic compounds as a soil amendment to control Fusarium wilt of cucumber caused by Fusarium oxysporum f. sp. cucumerinum, this study was conducted during the last two years from 1993 to 1995. According to add 14 inorganic chemicals (1%, w/v) including $Al_2(SO_4)_3$ individually in vitro, these chemicals were confirmed their suppression, and especially Alum, CaO and $Al_2(SO_4)_3$ suppressed not only $20.9{\sim}25.0$ percent on mycelial growth of the fungus, but also inhibited $72.8{\sim}97%$ on conidial germination. $Ca(NO_3)_2$ suppressed mycelial growth only, while KCl, $K_2SO_4,\;NH_4NO_3$, and Urea suppressed conidial germination. The 7 chemicals were finally seleced. Composted pine bark (CPB) suppressed definely more than 90% on conidial germination in the different extract concentration (2,5 and 10%), although mycelial growth on extract medium of CPB and milled alfalfa leaves (MAL) were not remarkable. The antagonist Trichoderma sp. (Tr-3) mixed with an amended soil (1%, w/w) containing composted pine bark showed a good mycelial growth to compete the causal fungus. And the antagonist Pseudomonas sp. (7-1-3) was also confirmed its antagonistic ability with culture filterate. It is known that a CPB soil amendment mixed with the two antagonists (1%, w/w) controlled almost completely Fusarium wilt of cucumber in greenhouse pots and a field experiment. It is therefore expected that biocontrol on Fusarium wilt of cucumber by a soil amendment can be applied to farmmer's fields.

• Korean Journal of Veterinary Research
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• v.40 no.2
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• pp.247-256
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• 2000

The regional distributions and relative frequencies of endocrine cells were studied histochemically (Grimelius and Masson-Hamperl (M-H) silver methods) in the alimentary tract of the red-eared slider, Trachemys scripta elegans. Samples were taken from the esophagus, fundus, pylorus, duodenum, jejunum, ileum and large intestine. Argyrophil (Grimelius-positive) cells and argentaffin (M-H-positive) cells were found in the whole alimentary tract in this study. Spherical to spindle and/or oval to round-shaped argyrophil or argentaffin cells were located in the gastric glands of the stomach regions, in the basal portion of the epithelium of intestinal tract or the esophagus with variable frequencies. Argentaffin cells were more numerously detected in the whole alimentary tract compared with those of argyrophil cells in this study. Argyrophil cells were observed in the whole alimentary tract including the esophagus and the most predominant region was the rectum with moderate frequency. The relative frequency of these cells was rare in the esophagus, fundus, duodenum, jejunum and ileum, respectively and a few frequency in the pylorus. Argentaffin cells were also observed in the whole alimentary tract including the esophagus and the most numerously demonstrated region was the rectum with numerous frequency. They were observed with a few frequencies in the remaining regions of the alimentary tract except for the rectum, respectively. However, to know the exact type of the argyrophil cells and argentaffin cells that were observed in this study, more developmental methods such as immunohistochemistry were needed.

• Korean Journal of Food Science and Technology
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• v.28 no.3
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• pp.552-557
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• 1996

Gels of rice starches isolated from jinmibyeo, llpoombyeo and Dongjinbyeo were prepared with $60{\sim}65%$ water content (based on the total weight) and stored at $-18^{\circ}C,\;4^{\circ}C,\;20^{\circ}C,\;30^{\circ}C,\;and\;37^{\circ}C$ for 6 days. The effects of varieties, water content and storage temperature on the degree of retrogradation (DR) of gels were measured by ${\alpha}$-amylase-iodine method. DRs of rice starch gels increased rapidly during the initial stage of storage, but slowly after that. DRs were highest with 60% water content and lowest with 65% water content at any storage temperature. Regarding the storage temperature, DRs of gels increased in the following order : $4^{\circ}C>20^{\circ}C>30^{\circ}C>37^{\circ}C>-18^{\circ}C>$. As the storage temperature was increased, DR per $10^{\circ}C$ increased above refrigerated temperature decreased in the following order : jinmibyeo>llpoombyeo>Dongjinbyeo.

• Korean Journal of Food Science and Technology
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• v.33 no.6
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• pp.645-650
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• 2001

A study was carried out to establish the detection method for irradiated cereals. Cereals were ground and irradiated at 2.5, 5.0, 7.5, 10, 15 kGy using a $Co^{60}$ irradiator. The viscosities decreased in all samples by increasing irradiation dose. The viscosity of the Panicum millaceum (Korean) and Andropogon sorghum (Korean) paste dropped from $143.38{\pm}0.44$ and $35.92{\pm}1.90$ in the control to $6.60{\pm}1.16$ and $3.86{\pm}0.32$, respectively, in the samples irradiated at 15 kGy. These trends were similar to samples from china. Regression equation and coefficients of viscosity of Panicum millaceum (Korean and China) and Andropogon sorghum (Korean and China) were 0.80 (y=-27.789x＋150.17), 0.98 (y=-3.367x＋88.93), 0.84 (y=-6.0466x＋35.49) and 0.84 (y=-13.346x＋101.67) at 50 rpm. All samples resulted in a decrease in specific parameter by increasing rpm after irradiation. Parameter values showed dose-dependent relationship between unirradiated and irradiated samples and indicated that all values of unirradiated samples were higher than the irradiated ones. These results suggest that the detection of irradiated cereals at various doses using viscometric methods is possible.