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http://dx.doi.org/10.7841/ksbbj.2014.29.1.58

Quality and Availability Evaluation of Human Hepatocytes Isolated from Resected Partial Livers for Toxicology and Drug Metabolism Studies in Korea  

Noh, Jeong-Kwon (Biomedical Research Center, Lifeliver Co. Ltd.)
Jang, In Keun (Biomedical Research Center, Lifeliver Co. Ltd.)
Kim, Hyo Eun (Biomedical Research Center, Lifeliver Co. Ltd.)
Lee, Jong Eun (Biomedical Research Center, Lifeliver Co. Ltd.)
Yang, Mal Sook (Biomedical Research Center, Lifeliver Co. Ltd.)
Jang, Eun Mi (Biomedical Research Center, Lifeliver Co. Ltd.)
Lee, Ji-Hyun (Samsung Biomedical Research Institute)
Park, Hey-Jung (Samsung Biomedical Research Institute)
Kim, Young-A (Samsung Biomedical Research Institute)
Lee, Suk-Koo (Department of Surgery, School of Midicine, Sungkyunkwan University)
Jeong, Ho-Sang (National Institute of Food and Drug Safety Evaluation, MFDS)
Ahn, Joon-Ik (National Institute of Food and Drug Safety Evaluation, MFDS)
Lee, Doo-Hoon (Biomedical Research Center, Lifeliver Co. Ltd.)
Publication Information
KSBB Journal / v.29, no.1, 2014 , pp. 58-66 More about this Journal
Abstract
Demand for in vitro pharmacological evaluation and toxicity test using human hepatocytes has been increasing. In USA and Europe, human hepatocytes obtained from donated whole liver unsuitable for transplantation were distributed to researchers and deposited in cell bank facility as cryopreserved vial. In Korea, however, incidence of transplantation- inappropriate whole liver has been quite low and the whole livers almost have so severe liver disease such as fatty or fibrotic liver that cannot meet the demand. In this study we aimed to isolate human hepatocytes from liver resection surgery-originated partial liver, and assure the isolated human hepatocytes and its cryopreserved hepatocytes to be qualified for the in vitro pharmacological evaluation and drug toxicity tests. We compared those with commercially available human hepatocyte, BD $GenTest^{TM}$ by cell morphology, hepatic gene expression, urea synthesis, albumin secretion, ammonia removal, and cytochrome P450 induction activities. Changes in hepatotoxic gene expression after cryopreservation are evaluated with a typical hepatotoxic drug, acetaminophen. Consequently, the fresh hepatocytes from the partial liver and its cryopreserved hepatocytes expressed their intrinsic hepatic functions well and showed equal hepatotoxicity gene expression trend regardless to cryopreservation. Therefore, liver resection surgery-originated partial liver can be used as a useful source of human hepatocytes for various pharmacological and hepatotoxicity test.
Keywords
Human hepatocyte; Partial liver; Drug metabolism; Drug screening;
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