• Title/Summary/Keyword: MAGE-3

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Analysis of 5-aza-2'-deoxycytidine-induced Gene Expression in Lung Cancer Cell Lines (폐암 세포주에서 5-aza-2'-deoxycytidine 처치에 의해 발현되는 암항원 유전자 분석)

  • 김창수;이해영;김종인;장희경;박종욱;조성래
    • Journal of Chest Surgery
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    • v.37 no.12
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    • pp.967-977
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    • 2004
  • Background: DNA methylation is one of the important gene expression mechanisms of the cell. When cytosine of CpG dinucleotide in promotor is hypomethylated, expression of some genes that is controlled by this promoter is altered. In this study, the author investigated the effect of DNA demethylating agent, 5-aza-2'-deoxycytidine (ADC), on the expressions of cancer antigen genes, MHC and B7 in 4 lung cancer cell lines, NCIH1703, NCIH522, MRC-5, and A549. Material and Method: After treatment of cell lines, NCIH1703, NCIH522, MRC-5 and A549 with ADC (1 uM) for 48 hours, RT-PCR was performed by using the primers of MAGE, GAGE, NY-ESO-1, PSMA, CEA, and SCC antigen gene. In order to find the optimal ADC treatment condition for induction of cancer antigen, we studied the effect of ADC treatment time and dose on the cancer antigen gene expression. To know the effect of ADC on the expression of MHC or B7 and cell growth, cells were treated with 1 uM of ADC for 72 hours for FACS analysis or cells were treated with 0.2, 1 or 5 uM of ADC for 96 hours for cell counting. Result: After treatment of ADC (1 uM) for 48 hours, the expressions of MAGE, GAGE, NY-ESO-1, and PSMA genes increased in some cell lines. Among 6 MAGE isotypes tested, and gene expression of MAGE-1, -2, -3, -4 and -6 could be induced by ADC treatment. However, CEA gene expression did not change and SCC gene expression was decreased by ADC treatment. Gene expression was generally induced 24 - 28 hours after ADC treatment and expression of MAGE, GAGE, and NY-ESO-1 was maintained at least 14 days after ADC ADC teatment, and expression of MAGE, GAGE, and NY-ESO-1 was maintained at least 14 days after ADC teatment in ADC-Free medium. Most gene expression could be induced at 0.2 uM of ADC, but gene expression increased dependently on ADC treatment dose. The expression of MHC and B7 was not increased by ADC treatment in all four cell lines, and the growth rate of 4 cell lines decreased significantly with the increase of ADC concentrations. Conclusion: Treatment of lung cancer cell lines with ADC increases the gene expression MAGE, GAGE and NY-ESO-1 that are capable of induction of cytotoxic T lymphocyte response. We suggest that treatment with 1 uM of ADC for 48 hours and then culturing in ADC-free medium is optimal condition for induction of cancer antigen. However, ADC has no effect on MHC and B7 induction, additional modification for increase of expression of MHC, B7 and cytokine will be needed for production of efficient cancer cell vaccine.

A Study on the Visual Interpretation of the Clothing Image as Clothing m Form and Dot Space Variation. (의복형태와 물방울무늬 공간변화에 따른 이미지의 시각적 평가)

  • 문삼련;이경희
    • Journal of the Korean Society of Clothing and Textiles
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    • v.18 no.1
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    • pp.3-14
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    • 1994
  • This study is intended to identify the clothing mage as clothing form and dot space variation. This study consists of pre-experiment for setting the space between dot which shows the difference of the image and main experiment and also is made of factorial design for two variables-clothing form(H-line, A-line, V-line, X-line) dot (dot 1.0, dot 2.2, dot 3.4, dot 5.8) Qestionaire consists of 21 semantic differential scale expressing clothing form and .dot. Data is obtained from 50 female students maforing clothing and textile. The results of this study are as follows. 1) The image by the clothing form and the space dot variation is composed 5 factors' attention, activity, attraction, maturity, boldness. 2) The image by variation in clothing form dot has significant differences in attractive and attention factors, especially shows remarkable differences in attention. By choosing narrow space dot for attentive image, broden space dot for attractive image, you would be able to create the image you want. 3) The image by variation in dot clothing form has almost signific and differences in all factors, especially shows remarkable differences in activity. By choosing A-line for active image, V-line for mature and fashionable image, X-line for attractive, pretty and delicate mage, you would be able to create the image you want. 4) The interaction effect between clothing form and space dot was in attraction and maturity factor, V-line, X-line, dot 3.4 and dot 5.8 intensify attractive image, V-line and dot 1.0 mature image, X-line and dot 5.8 young image.

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Gache(加髢) Culture and Position of East Asia Women in the 18th and 19th Centuries (18~19세기 동아시아 여성의 가체문화와 의미)

  • Yim, Lynn
    • Human Ecology Research
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    • v.57 no.3
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    • pp.395-406
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    • 2019
  • This study examined what meaning East Asian women showed in their costume history through a discourse of hair adornments such as wigs and that Gache was not just a luxury decoration. In addition, we examined Gache hair trends with Eonjeun-meori (braid wraps around the entire head) in the Joseon dynasty (Korea), Gigye(旗?) hair in the Quing dynasty (China) and Mage(?) hair in the Edo period (Japan) during the $18^{th}$ and $19^{th}$ centuries. The significance of the phenomenon of East Asian Gache culture in the $18^{th}$ and $19^{th}$ centuries was analyzed from the internal desires of women. The details are as follows. First, the magnification by the hair decoration was identified with self-authority and used as a sign to express self-respect or a desire for self-esteem. The extended Gache was an external body extension to raise self-authority and increase activeness. Second, self-satisfaction through showing off was associated with a women's search for identity. There was excessive consumption to boast status, wealth and femininity, but the mania continued because women obtained psychological satisfaction by feeling that their sacrifices for the Confucian order were compensated. Third, the frenzy of Gache was accepted as a way for women to resist social regulations and find themselves as main participants in social activities. Showing their appearance in East Asian Gache culture was a way of inner self-searching and a process for women to find themselves as a social entity.

A Study on the Garment Ease for Pre-School Children's Upper Clothing Construction (취학전 아동의 상의구성을 위한 여유량 연구)

  • 박찬미;서미아
    • The Research Journal of the Costume Culture
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    • v.1 no.2
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    • pp.145-157
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    • 1993
  • This study aimes to investigate the garment ease of pe-school children's clothing in accordance with arm movement. The experiment was done with 4 per-school children fro mage 3 to age 5, and 3 types of experimental clothes were made ; sleeveless, half sleeve, and long sleeve. The waist pattern and the sleeve pattern of each experimental clothes has no garment ease. And experimental clothes were examined to obtain the necessary ease of armcye line and waist lien by cross-cut method. The results of investigation can be summarized as follows ; 1. The resulting movement ranges of experimental clothes with no garment ease were 131.5°(sleeveless), 71.75°(half sleeve), and 62.25°(long sleeve). 2. The size order of cross-cut opening of waist lien movement were (side>front>back), and (half sleeve>long sleeve>sleeveless). 3. When the base point of measurement was set to armpit point by arm the latitudinal length of cross-cut opening of armcye line by arm movement was (anterior armpit part > posterior armpit part), and longitudinal length of cross-cut opening was (upper part>lower part).

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Lung Cancer Screening (폐암 조기 진단)

  • Kim, Ju Ock
    • Tuberculosis and Respiratory Diseases
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    • v.61 no.3
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    • pp.207-213
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    • 2006
  • 기존의 폐암 발생의 고위험군(나이. 흡연력, $FEV_1$, 가족력, 직업적 발암물질의 노출력, 기존의 폐암 및 두경부 종양)에 대한 저선량 나선식 CT와 기관지 내시경 검사로 선별 검사 시 폐암의 유병률은 높아야 2%내외이고 대부분 그보다 낮은 것으로 알려져 있다. 특이도는 49-90%, 양성예측률은 10%이하로 선별검사 시 불필요한 검사를 초래하고 그에 따른 이환률과 사망률을 증가시키며 비용적인 문제를 야기하는 것으로 알려져 있다. 이에 기존의 고위험군에 대해 대상 환자를 더욱 더 좁힐 필요가 있으며, 이는 실제 임상적으로 이용 가능한 생물학적 표지자의 개발의 필요성이 있다고 하겠다. 그러나 현재까지 알려진 폐암조기검진에 대한 각종 진단 수기 중애서 상기의 3가지 방법(저선량 MDCT, 자가형광기관지경 및 객담내 MAGE)을 한꺼번에 시행하는 program은 비용적인 문제는 있지만 시도해 볼 만한 방법이라고 생각된다.

A Method of Measuring Wood Failure Percentage of Wood Specimens Bonded with Melamine-Urea-Formaldehyde Resins Using I mage Analysis

  • KIM, Minseok;PARK, Byung-Dae
    • Journal of the Korean Wood Science and Technology
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    • v.49 no.3
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    • pp.274-282
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    • 2021
  • Transparent and colorless melamine-urea-formaldehyde (MUF) resins make it difficult to identify the area of wood failure percentage (WFP) in the fracture surface of bonded wood specimens. Therefore, in this study, we develop a method of measuring WFP after the adhesion strength measurement of MUF resins under shear stress. The fractured wood surface of b lock shear strength (BSS) specimens bonded with cold-setting MUF resins at three melamine contents (20%, 30%, and 40%) was marked black, and then, WFP was accurately measured via image analysis. WFP values measured using this method consistently increased with BSS as the melamine content increased, showing the reliability of this new method. The results suggested that this new method is useful and reliable for measuring the WFP of the fracture surface of wood specimens bonded with colorless adhesives such as urea-formaldehyde, MUF, and melamine-formaldehyde resins.

A study on hand gesture recognition using 3D hand feature (3차원 손 특징을 이용한 손 동작 인식에 관한 연구)

  • Bae Cheol-Soo
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.10 no.4
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    • pp.674-679
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    • 2006
  • In this paper a gesture recognition system using 3D feature data is described. The system relies on a novel 3D sensor that generates a dense range mage of the scene. The main novelty of the proposed system, with respect to other 3D gesture recognition techniques, is the capability for robust recognition of complex hand postures such as those encountered in sign language alphabets. This is achieved by explicitly employing 3D hand features. Moreover, the proposed approach does not rely on colour information, and guarantees robust segmentation of the hand under various illumination conditions, and content of the scene. Several novel 3D image analysis algorithms are presented covering the complete processing chain: 3D image acquisition, arm segmentation, hand -forearm segmentation, hand pose estimation, 3D feature extraction, and gesture classification. The proposed system is tested in an application scenario involving the recognition of sign-language postures.

Yellow Image and Formative Properties in Modern Fashion (현대패션에 나타난 노랑의 조형성과 이미지)

  • 오해순;이경희
    • Journal of the Korean Society of Clothing and Textiles
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    • v.26 no.6
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    • pp.865-876
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    • 2002
  • The purpose of the study is to clarify yellow image and formative properties in modern fashion. For the study of formative properties 230 kinds of costume samples being visual power in yellow have been selected from photographs in fashion magazines and divided into tones: vivid(S, B, Dp), vague(L, Lgr, D), bright(Vp, P, B). For the study of image 30 kinds of costume samples is used. The Study was measured by using Semantic Differential method. The subjects were 50 students majoring in clothing and textile. The data were analyzed by factor analysis, ANOVA, MDS and regression analysis. The results of analysis are as follow: 1. Factor analysis has extracted 4 factors of yellow image in the fashion. These factor are Attractiveness, Cheerfulness, Hardness and Softness, Gorgeousness. 2. There were significant difference in visual evaluation of yellow tones. 3. Evaluative dimensions of yellow was classified as Soft-Hard, Gorgeous-Unpretentious. 4. The mage effect on Preference, Buying needs, Pleasant and Riches was consist of complicated sensibility.

Xperanto: A Web-Based Integrated System for DNA Microarray Data Management and Analysis

  • Park, Ji Yeon;Park, Yu Rang;Park, Chan Hee;Kim, Ji Hoon;Kim, Ju Ha
    • Genomics & Informatics
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    • v.3 no.1
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    • pp.39-42
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    • 2005
  • DNA microarray is a high-throughput biomedical technology that monitors gene expression for thousands of genes in parallel. The abundance and complexity of the gene expression data have given rise to a requirement for their systematic management and analysis to support many laboratories performing microarray research. On these demands, we developed Xperanto for integrated data management and analysis using user-friendly web-based interface. Xperanto provides an integrated environment for management and analysis by linking the computational tools and rich sources of biological annotation. With the growing needs of data sharing, it is designed to be compliant to MGED (Microarray Gene Expression Data) standards for microarray data annotation and exchange. Xperanto enables a fast and efficient management of vast amounts of data, and serves as a communication channel among multiple researchers within an emerging interdisciplinary field.

Design and Implementation of JPEG Image Display Board Using FFGA (FPGA를 이용한 JPEG Image Display Board 설계 및 구현)

  • Kwon Byong-Heon;Seo Burm-Suk
    • Journal of Digital Contents Society
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    • v.6 no.3
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    • pp.169-174
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    • 2005
  • In this paper we propose efficient design and implementation of JPEG image display board that can display JPEG image on TV. we used NAND Flash Memory to save the compressed JPEG bit stream and video encoder to display the decoded JPEG mage on TV. Also we convert YCbCr to RGB to super impose character on JPEG image. The designed B/D is implemented using FPGA.

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