• Title/Summary/Keyword: MAE

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Microwave-assisted extraction of paclitaxel from plant cell cultures (Microwave를 이용한 식물세포배양으로부터 paclitaxel 추출)

  • Hyun, Jung-Eun;Kim, Jin-Hyun
    • KSBB Journal
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    • v.23 no.4
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    • pp.281-284
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    • 2008
  • A simple and efficient microwave-assisted extraction procedure was developed and optimized for the extraction of paclitaxel from the plant cell cultures of Taxus chinensis. The biomass, immersed in a methanol-water mixture, was irradiated with microwaves in a closed-vessel system. The microwave-assisted extraction was compared with the existing conventional solvent extraction in terms of yield, extraction time, and solvent consumption. The use of microwave energy allows rapid recovery of paclitaxel from biomass and dramatically reduces extraction time and solvent usage compared to conventional solvent extraction. The paclitaxel was completely extracted from biomass by microwave-assisted extraction for 3 min at $50^{\circ}C$, for 6 min at $30^{\circ}C$ and $40^{\circ}C$, respectively.

Effect of Microwave Assisted Water Extraction on Insoluble Phenolic Compounds through Bioactivity of Fagopyrum esculentum

  • Kim, Hyun Gi;Kim, Yong Ju;Lian, Thang Tung;Song, Sueng Yeob;Bang, Kuek Soo
    • Korean Journal of Plant Resources
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    • v.32 no.6
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    • pp.723-729
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    • 2019
  • Fagopyrum esculentum (Buckwheat) is a globally used alternative crop that contains several useful substances with various effects; however, many of these substances (rutin, quercetin, etc.) are water insoluble. To extract these substances, alcohols is required, which is inconvenient because these solvents cause diverse problems. Many studies are underway to achieve effective extraction of these substances with water. Among of these studies, microwave assisted water extraction (MAE) has been performed extensively. In this study, we performed the extraction in various solvents and/or microwave from Fagopyrum esculentum. The analysis of the content of useful substances and the bioactivity were performed and shown to increase in MAE. Liquid chromatography-mass was performed in order to identify of the useful water-insoluble substances. Catechin, quercetin, and rutin, which are all insoluble in water, were hardly extracted with water even on heating (4.4 ppb, 3.9 ppb and 60.3 ppb, respectively). However, MAE was found to extract much more of these substances than water (1204 ppb, 110.8 ppb and 2946 ppb, respectively). Although less efficient than alcohols, MAE showed much higher efficiency than simple water extraction. These results indicate that water extraction using microwave technology is effective in cases where it is difficult to extract useful substances using water.

Fast Motion Estimation Using the Statistical Characteristics of Motion Vector (움직임 벡터의 통계적 특성을 이용한 고속 움직임 추정)

  • Choi, Jung-Hyun;Park, Dae-Gyue;Lee, Kyeong-Hwan;Lee, Bub-Ki;Kim, Duk-Gyoo
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.37 no.2
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    • pp.21-27
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    • 2000
  • In Fast motion estimaion algorithms, they reduce the computational complexity using the assumption that the matching error increases monotonically as the search moves away from the global minimum error In this paper, we first investigate the statistical characteristics of motion vector that the motion vector mostly occures on the side of small MAE (mean absolute error) between the reference search points when the MAE difference of them is large Therefore, we propose a fast motion estimation algorithm using this property and can reduce the number of search points The computer simulation result shows that the proposed method reduces computational complexity compared with conventional fast algorithms.

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Comparison of Gut Microbiota between Lean and Obese Adult Thai Individuals

  • Jinatham, Vasana;Kullawong, Niwed;Kespechara, Kongkiat;Gentekaki, Eleni;Popluechai, Siam
    • Microbiology and Biotechnology Letters
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    • v.46 no.3
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    • pp.277-287
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    • 2018
  • Current reports suggest that obesity is a serious global health issue. Emerging evidence has predicted strong links between obesity and the human gut microbiota. However, only a few such studies have been conducted in Asia, and the gut microbiota of lean and obese adult Asians remains largely unexplored. Here, we investigated the potential relationship between gut microbiota, body massindex (BMI), and metabolic parameters in adults from Thailand, where obesity is increasing rapidly. Fecal and blood samples were collected from 42 volunteers who were allocated into lean, overweight, and obese groups. The fecal microbiota was examined by quantitative PCR analysis. Bacteroidetes, Firmicutes, and Staphylococcus spp. and methanogens were most abundant in lean volunteers. Overweight volunteers majorly harbored Christensenella minuta and Akkermansia muciniphila, ${\gamma}-Proteobacteria$, and bacteria belonging to the genus Ruminococcus. Methanogens and bacteria belonging to the phylum Bacteroidetes were negatively correlated with adiposity markers (BMI and waist circumference), but positive correlated with high-density lipoprotein, suggesting that they can be used as leanness markers. While some of our results agree with those of previous reports, results regarding the contributions of specific taxa to obesity were inconsistent. This is the first study to report the adult gut microbiota in Southeast Asian populations using molecular techniques and biochemical markers and provides a foundation for future studies in this field.

Solar Energy Prediction Based on Artificial neural network Using Weather Data (태양광 에너지 예측을 위한 기상 데이터 기반의 인공 신경망 모델 구현)

  • Jung, Wonseok;Jeong, Young-Hwa;Park, Moon-Ghu;Seo, Jeongwook
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2018.05a
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    • pp.457-459
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    • 2018
  • Solar power generation system is a energy generation technology that produces electricity from solar power, and it is growing fastest among renewable energy technologies. It is of utmost importance that the solar power system supply energy to the load stably. However, due to unstable energy production due to weather and weather conditions, accurate prediction of energy production is needed. In this paper, an Artificial Neural Network(ANN) that predicts solar energy using 15 kinds of meteorological data such as precipitation, long and short wave radiation averages and temperature is implemented and its performance is evaluated. The ANN is constructed by adjusting hidden parameters and parameters such as penalty for preventing overfitting. In order to verify the accuracy and validity of the prediction model, we use Mean Absolute Percentage Error (MAPE) and Mean Absolute Error (MAE) as performance indices. The experimental results show that MAPE = 19.54 and MAE = 2155345.10776 when Hidden Layer $Sizes=^{\prime}16{\times}10^{\prime}$.

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Freshwater Snail Diversity in Mae Lao Agricultural Basin (Chiang Rai, Thailand) with a Focus on Larval Trematode Infections

  • Chantima, Kittichai;Suk-ueng, Krittawit;Kampan, Mintra
    • Parasites, Hosts and Diseases
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    • v.56 no.3
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    • pp.247-257
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    • 2018
  • The aim of this study was to conduct a freshwater snail survey in Mae Lao agricultural basin to assess the diversity with a focus on habitat types and their larval trematode infections. Snails were collected and examined in 14 sites of Mae Lao agricultural basin from August 2016 to October 2017. A total of 1,688 snail individuals were collected and classified into 7 families, 8 genera, and 12 species. Snail diversity and habitat types were higher in rice paddies than irrigation canals and streams. The most abundant species was Bithynia siamensis siamensis, representing 54.6% of the sample. Three species of snails act as first intermediate host were found with cercarial infections. They were Filopaludina sumatrensis polygramma, B. s. siamensis, and Melanoides tuberculata. The cercariae were categorized into 7 types; echinostome, monostome, gymnocephalous, virgulate, parapleurolophocercous, pleurolophocercous and megalurous cercariae. Parapleurolophocercous cercariae constituted the most common type of cercariae recovered, contributing 41.2% of all infections in snails. Echinostome metacercariae infections were found in 6 snail species with 7.6% prevalence. In addition, the metacercaria of avian trematode, Thapariella sp. were found in Filopaludina spp. snails and B. funiculata with a prevalence of 0.5%. This is the first report for Thapariella metacercariae in the snail host, B. funiculata, and also confirmed that viviparid and bithyniid snails act as the second intermediate hosts of this trematode. This work will provide new information on the distribution and intermediate host of trematode in this area.

An optimized microwave-assisted extraction method for increasing yields of rare ginsenosides from Panax quinquefolius L.

  • Yao, Hua;Li, Xuwen;Liu, Ying;Wu, Qian;Jin, Yongri
    • Journal of Ginseng Research
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    • v.40 no.4
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    • pp.415-422
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    • 2016
  • Background: Rare ginsenosides in Panax quinquefolius L. have strong bioactivities. The fact that it is hard to obtain large amounts of rare ginsenosides seriously restricts further research on these compounds. An easy, fast, and efficient method to obtain different kinds of rare ginsenosides simultaneously and to quantify each one precisely is urgently needed. Methods: Microwave-assisted extraction (MAE) was used to extract nine kinds of rare ginsenosides from P. quinquefolius L. In this article, rare ginsenosides [20(S)-Rh1, 20(R)-Rh1, Rg6, F4, Rk3, 20(S)-Rg3, 20(R)-Rg3, Rk1, and Rg5] were identified by high performance liquid chromatography (HPLC)-electrospray ionization-mass spectrometry. The quantity information of rare ginsenosides was analyzed by HPLC-UV at 203 nm. Results: The optimal conditions for MAE were using water as solvent with the material ratio of 1:40 (w/v) at a temperature of $145^{\circ}C$, and extracting for 15 min under microwave power of 1,600 W. Seven kinds of rare ginsenosides [20(S)-Rh1, 20(R)-Rh1, Rg6, F4, Rk3, Rk1, and Rg5] had high extraction yields, but those of 20(S)-Rg3 and 20(R)-Rg3 were lower. Compared with the conventional method, the extraction yields of the nine rare ginsenosides were significantly increased. Conclusion: The results indicate that rare ginsenosides can be extracted effectively by MAE from P. quinquefolius L. in a short time. Microwave radiation plays an important role in MAE. The probable generation process of rare ginsenosides is also discussed in the article. It will be meaningful for further investigation or application of rare ginsenosides.

Changes of Total Polyphenol Content and Electron Donating Ability of Aster glehni Extracts with Different Microwave-assisted Extraction Conditions (마이크로웨이브 추출조건에 따른 섬쑥부쟁이 추출물의 총 폴리페놀 함량 및 전자공여 작용 변화)

  • Kim, Hyun-Ku;Kwon, Young-Joo;Kim, Kong-Hwan;Jeong, Yoon-Hwa
    • Korean Journal of Food Science and Technology
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    • v.32 no.5
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    • pp.1022-1028
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    • 2000
  • Aster glehni was extracted by microwave-assisted extraction(MAE) and reflux extraction(RE) methods and their extraction efficiencies were compared. A considerable reduction in extraction time was achieved by MAE. When 70% methanol, 50% methanol, 70% ethanol, or 50% ethanol was used, MAE extract contained nearly same amounts of soluble solid and total polyphenol contents as obtained by RE. The optimum MAE conditions for the extraction of Aster glehni were $120{\sim}150$ watts of microwave energy and $4{\sim}8$ minutes of extraction time. No significant changes were found in electron donating ability(EDA) over the variation of microwave energy or extraction time. The use of diluted methanol or ethanol resulted in improving extraction yield(24%), total polyphenol content(2.6%) and EDA(60%).

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