• The Korea Journal of Herbology
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• v.29 no.2
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• pp.15-21
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• 2014

Objectives : The purpose of this study was to investigate inhibitory effects of steamed Polygonatum odoratum extract (POE) on differentiation and adipogenesis in 3T3-L1 adipocytes. Methods : Polygonatum odoratum (P. odoratum) extract was extracted with ethyl acetate. Total phenolic and flavonoid contents in POE were measured for antioxidant activity. The spectrophotometric method was used to determine the DPPH and ABTS radical scavenging activity and ferric-reducing antioxidant potential (FRAP). MTT assay was examined for cell toxicity, oil red O staining was performed for intracelluar adipogenesis in differentiated 3T3-L1 adipocytes. Western blot analysis for measurement of CCAAT/enhancer-binding protein ${\alpha}$ ($C/EBP{\alpha}$), peroxisome proliferator-activated receptor${\gamma}$ ($PPAR{\gamma}$) and AMP-activated protein kinase (AMPK) expressions were performed. Results : The results revealed that POE has antioxidant activities. Contents of total polyphenolics and flavonoids were $50.83{\pm}1.52$ GAE mg/100g dry weight of POE and $17.05{\pm}2.47$ RE mg/100g dry weight of POE, respectively. DPPH radical scavenging activity, and FRAP in 10 mg/ml concentration were $92.1{\pm}0.6%$, $244.8{\pm}9.0{\mu}M$ Fe(II) and ABTS inhibition in 5 mg/ml concentration was $84.8{\pm}4.1%$. Treatment of POE in adipocytes inhibited the differentiation and adipogenesis of 3T3-L1 adipocytes compared to those of vehicle control. Additionally, protein expressions of $C/EBP{\alpha}$ and $PPAR{\gamma}$, major transcription factor for the adipogenic genes, were significantly decreased compared to those of vehicle control (p<0.05). Futhermore, phosphorylation of AMPK was increased in 3T3-L1 adipocytes treated with POE compared to that of vehicle control (p<0.05). Conclusions : we demonstrate that steamed P. odoratum extract (POE) has potentiating antioxidant activities, inhibits differentiation and lipid accumulation and also induces energy expenditure in adipocytes, which may contribute to antiobesity property.

• Journal of People, Plants, and Environment
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• v.24 no.2
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• pp.169-178
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• 2021

Background and objective: Education based on agricultural experience in school gardens is coming to the fore as a solution to reduce the negative effect of rapid urbanization and the development of information technology (IT) on students. Accordingly, this study aimed to investigate how parents of elementary, middle, and high school students in Korea perceive the value of education service using school gardens, as well as their willingness to participate in and pay for such service, and to utilize the results in a quantitative assessment of the socioeconomic value of the education service. Methods: A contactless online survey on the perceived value of education service using school gardens was conducted on 1,010 parents of elementary, middle, and high school students in Korea's five major districts from October 22 to 26 (5 days) of 2020 by M, a Korean specialized survey agency. The data collected was analyzed using SPSS for Windows 25.0 and Excel to obtain the frequency and ratio of each measured item. Results: The respondents had an average of 1.83, of which 52.8% were male. 55.3% of the respondents were aware of the education service using school gardens, and 27.9% experienced the service. When asked if they saw the educational service using school gardens as necessary, 79% answered in the affirmative. In terms of the difference in perception of the need for the service according to experience, 91.8% of the parents who experienced the service responded that it was necessary, indicating that it is important to provide opportunities to experience this service to expand the scope of service. 54.9% of all respondents responded they were willing to pay the costs required for school gardens. When income tax was used as a payment method, the average amount parents were willing to pay was 13,193 KRW, and the tax rate was 2.02%. Based on experience, those who experienced this service had a higher need and willingness to pay for the service than those who had not experience, but the actual amount or tax rate was low as they knew how it was operated. Conclusion: As basic research on the assessment of the value of agricultural experience education using school gardens, this study determined how parents perceive this form of education service as well as their willingness to participate and pay for the service. These findings can be used to systemize the assessment indicators and promote the value of the education service using school gardens, allowing students to maintain a healthy and happy school life through agricultural experience.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.36 no.4
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• pp.360-365
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• 1991

This experiment was conducted to determine mechanized seeding ability of barley drill seeder on the seeding time, growth and yield of sorghum-sudangrass hybrid. The time required for machine drill seeding was 42 minutes per l0a which was one-thirteenth of the time required by the manual drill seeding, and percentage of seedling establishment by machine drill seeding was better than that of manual drill seeding. Leaf area index in seeding rate with 30 x 5cm and 40 x 5cm of machine drill seeding was higher than those of manual drill seeding, the leaf distribution was better and the total nonstructural carbohydrate content of stubble after cutting was short increased than that of manual drill seeding, Content of feed composition was not significantly different between manual and machine drill seeding. At seeding rate of 40 x 5cm of machine seeding. dry matter and TDN yield were increased to 29% and 30% respectiyely, than those of manual drill seeding.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.27 no.1
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• pp.78-83
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• 1982

Seedling blight of sesame (Sesamum indicum L.) is one of the most serious diseases in Korea. This study was performed to control seedling blight in the field where sesame has been cultured for 4 years of Industrial Crop Division at the Crop Experiment Station. The pathogenic fungi of seedling blight which is known Fusarium oxysporum f. vasinfectium and Rhizoctonia solani was inoculated to the wheat media and it was spreaded on the experimental field in the concentration of 109 of wheat media mixed with 10g of fine soil. Seed sterilization was the most effective method for seedling blight control for two year experiment. Seed sterilant Benlate-T was highly effective for con trolling seedling blight with infection rate of 4% and yield of 40.7kg per 10a comparing to the control with infection rate of 96% and grain yield of 6.4kg per l0a, the other chemicals, Busan 30, Vitathiram, Captan showed good effect to control seedling blight, but less effect to the Benlate-T. The spraying treatments on leaf surface 25 days after sowing were not effective to seedling blight.

• Journal of Korean Foot and Ankle Society
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• v.26 no.1
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• pp.40-47
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• 2022

Purpose: The modified Broström repair (BR) technique has yielded good outcomes in patients with chronic ankle instability. This study compared clinical and radiological outcomes between two groups of patients who underwent modified BR or lateral ligament augmentation using suture tapes (ST). Materials and Methods: Seventy-seven patients (ST group [n=47], BR group [n=30]; body mass index <26.61 kg/m²; mean age, 30.7±11.0 years [range, 17~39 years]; mean follow-up, 34.0±12.0 months [range, 24~59 months]) were retrospectively reviewed between January 2014 and July 2017. The Foot and Ankle Outcome Score (FAOS), American Orthopedic Foot and Ankle Score (AOFAS), Foot and Ankle Ability Measure (FAAM), visual analogue scale (VAS) score, and Sefton grading system were used for clinical assessment. The talar tilt angle and anterior talar translation were measured using the Telos stress device (Telos GmbH, Marburg, Germany) at 150 N for radiological evaluation. Results: FAOS, AOFAS, FAAM, and VAS scores improved in both groups at final follow-up (ST, 91.1±5.2, 93±2, 88.1±4.5, 1.5±0.7 vs. BR, 91.3±5.4, 93±3, 83.3±4.8, 1.2±0.7, respectively; p=0.854, 0.971, <0.001, 0.04, respectively). According to the FAOS, mean sports activity scores for the ST and BR groups at the final follow-up were 90.3±3.2 and 76.6±4.2, respectively, reflecting superior outcomes in the ST group (p<0.001). Sefton grading revealed satisfactory functional outcomes (ST, 91.5% vs. BR, 90.0%). There was significant improvement in the talar tilt angle and anterior talar translation in both the ST and BR groups (7.6°±1.2°, 10.5±1.8 mm vs. 4.9°±1.1°, 7.9±1.5 mm, respectively; p<0.001). Conclusion: The ST group demonstrated comparable clinical but better improvement in mechanical stability and FAOS sports scores than the BR group.

• Korean journal of applied entomology
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• v.62 no.1
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• pp.43-48
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• 2023

The efficacy of spinetoram sprayed from an unmanned helicopter to control two insect vectors of pine wood nematodes, Monochamus alternatus and M. saltuarius was assessed. By using a ULV (Ultra Low Volume) sprayer, the mortality rates of phenthoate, bifenthrin, etofenprox, and diflubenzuron, which are registered for use against coleopteran insects, indoxacarb and spinetoram, which are registered for use against lepidopteran insects, were evaluated for efficacy as aerial spraying agents. The ULV test, using 33- and 55-fold dilutions of phenthoate, bifenthrin, indoxacarb, and spinetoram demonstrated a 100% insect mortality rate upon contact and feeding mortality on the third day after treatment, while etofenprox and diflubenzuron exhibited a slightly lower mortality rate than the remaining compounds. Consequently, spinetoram was selected as a candidate pesticide for aerial spraying and its efficacy to control the insect vectors was assessed. The x 33 dilution of spinetoram resulted in a 98.6-100% control efficacy against two insect vectors. However, risk assessment for bees following aerial spraying will be necessary before applying the pesticide to control insect vectors.

• Journal of Animal Science and Technology
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• v.65 no.4
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• pp.779-791
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• 2023

This study aimed to assess the effects of embryonic developmental stage, quality grade, and fresh or frozen/thawed conditions on the pregnancy rate and sex ratio of live offspring in Hanwoo (Bos taurus coreanae) cows. The quality and developmental stage of in vivo-derived (IVD) transferred embryos were evaluated using the standard criteria of the International Embryo Technology Society. The recipient cows were synchronized using conventional (estradiol benzoate and progesterone) protocols before embryo transfer. Embryos were transferred to 297 cows, and pregnancy was monitored for 60-70 days after embryo transfer. The pregnancy rates of fresh and frozen/thawed embryos were 56.90% and 52.49%, respectively. Pregnancy rates varied according to embryo quality (56.18% for grade 1 vs. 36.67% for grade 2). Pregnancy rates also varied by developmental stage and cryopreservation (67.86% vs. 63.49% for stage 4-1, 64.00% vs. 54.72% for 5-1, and 50.00% vs. 47.83% for 6-1, in fresh embryos vs. frozen/thawed embryos, respectively). For stage 7-1, the pregnancy rates were 72.73% for fresh embryos and 20.00% for frozen/thawed embryos. In 66 fresh embryos, the sex ratio of live offspring was 5:5, whereas it was 4(female):6(male) for frozen/thawed embryos among the 95 frozen/thawed embryos. The miscarriage rate was approximately 3% higher for frozen/thawed embryos than for fresh embryos (18.1% for fresh vs. 21.1% for frozen). Seasonal fertility rates were 33.3% in spring, 55.67% in summer, 52.8% in autumn, 60.0% in winter. The following male-to-female ratios were observed in different seasons: 6.7:3.3 in spring, 4.0:6.0 in summer, 5.5:4.5 in autumn, and 3.3:6.7 in winter. The current data revealed no significant differences in pregnancy rates between fresh and frozen/thawed IVD embryos. However, there was a lower pregnancy rate with advanced-stage frozen/thawed embryos (stage 7-1). The current study provides comprehensive results for the better optimization of embryo transfer in Hanwoo cattle to obtain the desired fertility rate, pregnancy rate, and sex ratio of calves. These results provide important insights into the factors that influence the viability and success of IVD embryo transfer in Hanwoo cows and may have practical applications for improving breeding programs and reducing production costs.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.81-81
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• 2022

잇꽃(Carthamus tinctorius L.)은 국화과에 속하는 두해살이 초본 식물로 한자명은 홍화(紅花), 영명으로는 Safflower를 사용하고 있다. 우리나라의 잇꽃 재배는 2010년 39ha 수준이 재배되다가 2014년에는 76ha까지 확대되었으나, 점차 감소하는 추세를 보이고 있으며 2020년에는 52ha가 재배되고 있는 것으로 보고되었다. 잇꽃은 종자를 약용으로 이용하거나 꽃잎을 건조하여 천연 염색제 및 향신료로 이용하고 있으나, 일본 산형(山形)현에서는 잇꽃의 경관적 가치를 이용해 홍화 축제를 개최함으로써 소득작목으로 자리매김하고 있다. 본 연구는 지리산을 중심으로 한 준고랭지 지역에서 잇꽃의 경관적 가치의 활용 가능성을 검토하고자, 국립농업유전자원센터로부터 총포에 가시가 없는 무가시형 유전자원(IT323225, IT333473, IT333482)을 분양받아 가시가 있는 재배종과 파종시기별로 개화 특성 등을 비교하였다. 표고 500m 준고랭지의 비가림 하우스에서 3월 하순부터 6월 상순까지 파종시별로 출현율을 검토한 결과, IT 323225 자원은 관행의 3월 하순 파종에서도 40.5%의 낮은 출현율을 보였다. 3월 하순 파종에서 재배종은 6월 22일경에 개화기에 도달하였으나, 무가시형 유전자원들은 7월 2일~5일경에 개화기에 도달하여 만생종의 특성을 보였다. 파종시기를 늦춰 5월 하순에 피종하는 경우 무가시형 자원들은 7월 27일~29일경에 개화기에 도달하였으며, 개화기 전후의 개화 지속기간은 8~10일이 소요되어 3월 하순에 파종하여 7월 상순에 개화하는 경우보다 2~4일이 단축되는 경향이었다. IT333473 자원의 개화기 초장은 5월 하순 파종하는 경우 관행의 3월 하순 파종보다 43.6%가 즐어든 71.1cm를 보였고, 분지수는 41.6% 감소한 8.0개/주 수준을 보였다. 식물체당 착화수 또한 파종시기를 늦춤에 따라 감소하는 경향이었으나 화당 종자수는 IT323225 자원은 5월 중순과 하순 파종시에 증가하는 양상이었고 IT333473 자원과 IT333482 자원은 재배종과 같이 감소하였다. 하계 휴양지로 각광받고 있는 지리산권에서 무가시형 잇꽃 자원의 경관적 가치 활용 가능성을 검토한 결과, 파종시기를 5월 하순으로 늦추는 경우 성수기인 7월 하순과 8월 상순에 개화가 가능하였다. 또한 식물체의 초장이 71.1~83.6cm 수준으로 단축되어 경관 조성에 유리한 특성을 보였다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.11-11
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• 2020

Background : The genus Cypripediums are typically adapted to the low temperature and cold climates of the high land in Korea. The species which were reported in Korean peninsula are C.japonicum, C.macranthos, C.calceolus and C.guttatum. We had already reported the successful germination of the C.macranthos and C.guttatum. The small spotted lady's slipper orchid(C.guttatum) is most endangered plants to extinction of which population was reported less than 100 in Korea. The objective of the present research is restoration of the C.guttatum in ex situ and adaptation in high land and nursery. Methods and Results : The 350 seedlings of C.guttatum were transplanted in soil at alpine area(altitude 750m) and the nursery of Korea National Arboretum(KNA) in YangPyeong city on May 2019 and 2020 in order to verify the possibility of ex situ restoration. The sprouted ratio of the seedlings were less than 10% in the alpine area and 83% in the nursery of KNA. The reasons of the diverse survival ratio are presumed as the low moisture content in the soil and heavy shade of the alpine area. The 15 cypripedium species and 29 hybrids were also effectively adapted in the KNA rare plant garden. Conclusion : These results demonstrated the difficulties of the restoration for small spotted lady's slipper orchid in the natural habitation. From the above results, we may conclude that the critical factors of the genus Cypripedium are intensive care for the fragile seedlings during the 1^st year acclimatization.

• The Journal of Korean Obstetrics and Gynecology
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• v.37 no.1
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• pp.75-88
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• 2024

Objectives: This study aimed to identify the current use of Korean medicine for obesity and its effect for women in climacteric period. Methods: We studied women aged 45-55 who visited Daejeon Korean medicine Hospital of Daejeon University to lose body weight from January 1, 2021 to December 31, 2022 via an analysis of the medical records. The treatment duration was continuous for more than 2 weeks, and a body composition was measured by Inbody 770 at 2 to 4 weeks after the first visit. Results: 28 patients were finally selected and their average age was 49.32±3.38 years. Based on the body mass index (BMI) classification, 19 were in the 1st obesity group, 5 in the 2nd obesity group, 3 in the overweight group and 1 in the normal group. Patients usually complained comcomitant symptoms, and the symptoms of menopausal disorder was the most frequent. The average treatment duration was 3.68±0.67 weeks and the average treatment frequency was 3.93±0.98 times. All patients took herbal medicines Gambi-tang and 23 took modified fasting therapy including Gamro-su. 14 were treated by whole body far-infrared therapy and 6 were gotten partial obesity treatment. Among patients treated for accompanying symptoms, menopausal disorders were the most common (35.71%), and herbal medicine such as Gamisoyou-san, Hominis Placenta Pharmacopuncture, moxibustion, and cupping were used. After treatment, on average, body composition changed significantly; body weight 3.28±1.82 kg, BMI 1.36±1.04 kg/m², body fat 1.70±1.67 kg, skeletal muscle mass 0.81±0.91 kg, abdominal circumference 2.04±2.6 cm, and visceral fat area 8.91±12.83 cm². Conclusions: We analyzed general characteristics, BMI distribution, types of Korean medicine treatment and change of body composition. This study could be used as reference to provide practical data of treatment for obese women in climacteric period.