• 종축개량
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• v.17 no.3
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• pp.70-83
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• 1995

• KOREAN POULTRY JOURNAL
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• s.11
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• pp.81-83
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• 1970

• KOREAN POULTRY JOURNAL
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• v.40 no.10
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• pp.82-83
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• 2008

• 대한예방의학회:학술대회논문집
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• 1994.02b
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• pp.74-83
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• 1994

• Journal of the Korean Geosynthetics Society
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• v.5 no.1
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• pp.33-38
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• 2006

Large-scale direct shear tests were conducted in order to evaluate both the shear strength of crushed stone itself and the interface shear strength between crushed stone and geotextile. Total three types of geotextile (i.e. one woven geotextile and two nonwoven geotextiles) were used in the experimental program, considering two different values for the unit weight of crushed stone. Total fifteen tests were conducted in this study. It has been found from the experimental results that the friction angles of crushed stone itself were $47^{\circ}$ and $57^{\circ}$ under the unit weights of crushed stone being $1.4t/m^3$ and $1.5t/m^3$, respectively. Interface friction angle between nonwoven geotextile and crushed stone showed $39^{\circ}$ for type A indicating an efficiency of 83% and $42^{\circ}$ for type B indicating an efficiency of 89%. Similarly, interface friction angle between woven geotextile and crushed stone showed $39^{\circ}$ indicating an efficiency of 83%.

• Korean Journal of Microbiology
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• v.28 no.2
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• pp.174-179
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• 1990

In order to overproduce L-phenylalanine, various kind of regulatory mutants were isolated from parental Escherichia coli K-12. MWEC 83 Producing 7.4g/l of L-phenylalanine has been derived as a tyrosine and tryptophan double auxotrophic mutant. To produce L-phenylalanine without adding L-tyrosine and L-tryptophan, revertant strain MWEC 101 was isolated from MWEC 83. Further various analogues and valine resistant mutants were isolated from MWEC 101. MWEC 101-5 was the most excellent strain that produced 17.9g/l of L-phenylalanine after having been cultivated for 54 hours in 15% glucose medium. It was disclosed that activities of rate-limiting enzymes including chorismate mutase and prephenate dehydratase in MWEC 101-5 were desensitized to 2mM L-phenylalanine in the enzyme reaction mixture and that activities level of 3-deoxy-D-arabino-heptulosonic acid-7-phosphate synthase and prephenate dehydratase were increased more than 20 times over those of the parental strain.

• Korean Journal of Plant Resources
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• v.17 no.3
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• pp.289-296
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• 2004

A cDNA clone (GenBank accession no. CF924621) homologous to aluminum induced protein gene was isolated and characterized from Codonopsis lanceolata (ClAIP). The ClAIP is 906 nucleotides long and has an open reading frame of 711 bp with a deduced amino acid sequence of 236 residues. The ClAIP shows high homology to A. marina (84%), G. hirsutum(83%), V. radiata (83%), A. thaliana (80%), B. nap us (78%) and T. aestivum (68%). The deduced amino acid sequence of ClAIP also has homology to the N-terminal end of plant Asn synthetase. This region does not contain the active sites of the enzyme and the significance of this conservation is currently not clear. To investigate the expression of ClAIP against several heavy metal stresses, we treated the sliced tap root of C. lanceolata with various heavy metals. The expression of ClAIP was increased by 25 uM $Al_2$(SO$_3$)$_4$ in proportion to incubation time and also increased by 50 uM CdCl$_2$.

• The Bulletin of The Korean Astronomical Society
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• v.44 no.1
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• pp.83.2-83.2
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• 2019

We study the stability of the magnetic structure in active region (AR) 12371 producing an M6.5 flare on June 22 2015. We first perform a nonlinear force-free fields (NLFFFs) extrapolation to derive three-dimensional (3D) magnetic fields based on time series of observed photospheric magnetic fields. The NLFFFs well describe an observed sigmoidal structure with the shape of a double arc magnetic configuration. Next, we examine three possible instabilities (kink, torus, and double arc) to investigate how the M6.5 flare is triggered in the double arc loops. Consequently, the double arc loops are stable against kink and torus instabilities, but possibly unstable against the double arc instability before the flare occurrence. Finally, we discuss a probable scenario for the M6.5 flare.

• Microbiology and Biotechnology Letters
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• v.20 no.1
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• pp.25-33
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• 1992

One acetolactate synthase isozyme which has Rf value of 0.83 on polyacrylamide gel electrophoresis was purified from Sewatia marcescens ATCC 25419 by ammonium sulfate fractionation, DEAE-Sephacel chromatography, Phenyl-Sepharose chromatography, Sephacryt S-400 gel filtration followed by native gel elution. The native molecular weight of the enzyme was determined to be 531,400 by gel filtration method, and SDS-polyacrylamide gel electrophoresis separated the native enzyme into two polypeptides having molecular sizes of 55,000 and 38,900 respectively. In kinetic parameters, $K_m$ value for pyruvate was 2.54 mM, and $V_{max}$ was 21.75 nmoie/min/mg. The enzyme showed maximal activity around pH 8.0 and optimal temperature of the acetolactate formation was $37^{\circ}C$. Feedback inhibition studies indicate that the purified enzyme is rather resistant to branched chain amino acids when compared with acetolactate synthase isozymes of plants or other enterobacteria.

• Journal of Mushroom
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• v.19 no.3
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• pp.150-159
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• 2021

Anti-melanogenesis and skin anti-wrinkle effects of methanol (ME) and hot water (HE) extracts from the fruiting bodies of Ganoderma applanatum were investigated in this study. The total phenolic contents of the ME and HE of the mushroom were 11.68 and 3.15 ㎍ GAEs/mg, respectively, whereas the total flavonoid contents of the ME and HE were 21.82 and 2.69 ㎍ QEs/mg, respectively. The survival rate of B16-F10 murine melanoma cells treated with 750 ㎍ ME and HE were 83.46% and 85.54%, respectively, thereby suggesting that mushroom extracts were slightly cytotoxic at the tested concentration. The in vitro tyrosinase inhibition by ME (83.15%) and HE (83.44%) was significantly lower than that of kojic acid (99.61%), the positive control, at 2.0 mg/mL. Although the inhibition of cellular melanin synthesis in B16-F10 melanoma cells by 2.0 mg/mL of ME (50.24%) and HE (51.24%) was lower than that of arbutin (64.84%), the inhibition by both ME and HE was higher than 50%. Collagenase inhibition by HE was comparable to 2.0 mg/mL epigallocatechin (EGCG), the positive control; however, elastase inhibition by ME and HE was lower than that of EGCG at the concentration tested. The results showed that the fruiting bodies of G. applanatum had good anti-tyrosinase, good anti-collagenase, and moderate anti-elastase activities, which might be useful for developing novel skin-whitening and anti-wrinkle agents.