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Isolation and Expression of Aluminum Induced Protein(ClAIP) Gene from Codonopsis lanceolata  

양덕춘 (경희대학교 한방재료가공학과)
김종학 (㈜바이오피아)
인준교 (㈜바이오피아)
이범수 (㈜바이오피아)
이강 (㈜바이오피아)
Publication Information
Korean Journal of Plant Resources / v.17, no.3, 2004 , pp. 289-296 More about this Journal
Abstract
A cDNA clone (GenBank accession no. CF924621) homologous to aluminum induced protein gene was isolated and characterized from Codonopsis lanceolata (ClAIP). The ClAIP is 906 nucleotides long and has an open reading frame of 711 bp with a deduced amino acid sequence of 236 residues. The ClAIP shows high homology to A. marina (84%), G. hirsutum(83%), V. radiata (83%), A. thaliana (80%), B. nap us (78%) and T. aestivum (68%). The deduced amino acid sequence of ClAIP also has homology to the N-terminal end of plant Asn synthetase. This region does not contain the active sites of the enzyme and the significance of this conservation is currently not clear. To investigate the expression of ClAIP against several heavy metal stresses, we treated the sliced tap root of C. lanceolata with various heavy metals. The expression of ClAIP was increased by 25 uM $Al_2$(SO$_3$)$_4$ in proportion to incubation time and also increased by 50 uM CdCl$_2$.
Keywords
aluminum stress; Asn synthetase; Codonopsis lanceolata; heavy metal; RT-PCR;
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