• Asian-Australasian Journal of Animal Sciences
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• v.28 no.3
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• pp.405-410
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• 2015

The objective of this study was to evaluate the effects of sequential applications of ${\varepsilon}$-polylysine (EPL) or lauramide arginine ethyl ester (LAE) sprays followed by an acidic calcium sulfate (ACS) spray on inoculated chicken carcasses to reduce Salmonella (Salmonella enterica serovars including Salmonella typhimurium and Salmonella enteritidis) contamination during 6 days of storage ($4.4^{\circ}C$). Secondly, reductions of the resident microflora were studied on uninoculated chicken carcasses following the sequential application of the treatments, chilling and 10 days of storage at $4.4^{\circ}C$. The treatment of Salmonella inoculated carcasses with 300 mg/L EPL followed by 30% ACS (EPL300-ACS30) sprays reduced Salmonella counts initially by 1.5 log cfu/mL and then by 1.2 log cfu/mL (p<0.05) following 6 days of storage at $4.4^{\circ}C$. Likewise, 200 mg/L LAE followed by 30% ACS (LAE200-ACS30) treatment reduced initial Salmonella counts on poultry carcasses by 1.8, 1.4 and 1.8 log cfu/mL (p<0.05), respectively, after 0, 3, and 6 days storage. Immediately after the treatments, EPL300-ACS30 and LAE200-ACS30 both reduced Escherichia coli counts significantly by 2.6 and 2.9 log cfu/mL, respectively. EPL300-ACS30 and LAE200-ASC30 were effective in lowering psychrotroph counts by 1 log cfu/mL on day 10 when compared to the control and distilled water treatments. This study demonstrated that EPL300-ACS30 and LAE200-ACS30 were effective in reducing Salmonella on inoculated chicken carcasses both after treatment and during the storage at $4.4^{\circ}C$ for up to 6 days. In addition, reductions in psychrotroph counts indicated that these treatments might have the potential to increase the shelf-life of poultry carcasses.

• Steel and Composite Structures
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• v.33 no.3
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• pp.433-444
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• 2019

Fatigue failure of a grid structure using bolt-sphere joints is liable to occur in a high-strength bolt due to the alternating and reciprocal actions of a suspension crane. In this study, variable amplitude fatigue tests were carried out on 20 40 Cr steel alloy M30 high-strength bolts using an MTS fatigue testing machine, and four cyclic stress amplitude loading patterns, Low-High, High-Low, Low-High-Low, and High-Low-High, were tested. The scanning electron microscope images of bolt fatigue failure due to variable amplitude stress were obtained, and the fractographic analysis of fatigue fractures was performed to investigate the fatigue failure mechanisms. Based on the available data from the constant amplitude fatigue tests, the variable amplitude fatigue life of an M30 high-strength bolt in a bolt-sphere joint was estimated using both Miner's rule and the Corten-Dolan model. Since both cumulative damage models gave similar predictions, Miner's rule is suggested for estimating the variable-amplitude fatigue life of M30 high-strength bolts in a grid structure with bolt-sphere joints; the S-N fatigue curve of the M30 high-strength bolts under variable amplitude loading was derived using equivalent stress amplitude as a design parameter.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.15 no.2
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• pp.171-177
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• 1982

Optical properties of sea water were studied in the western channel of the Korea Strait, based on the data obtained from fifteen oceanographic stations in July, 1980. Submarine daylight intensity was measured at intervals of 5m depth in the upper 70m layer by using the underwater irradiameter (Kahlsico $\#268_{WA}360$). The mean absorption coefficients of the sea water were shown as $0.098(0.063\sim0.183),\;0.129(0.090\sim0.270), 0.081(0.044\sim0.142),\;and 0.087(0.036\sim0,142)$ for clear, red, green, and blue color respectively. The transparency ranged from 11.5 to 24m(mean 18.3m). The mean water color in this area was $3.5(3\sim4)$ in Forel scales. The relation between absorption coefficient $(\kappa)$ and transparency (D) was $\kappa=1.72/D,\;\kappa=2.33/D,\;\kappa=1.41/D,\;and \kappa=1.44/D$ for clear, red, green, and blue color respectively. The rates of light penetration for clear, red, green, and blue color in four different depths were computed with reference to the surface light intensity respectively. The mean rates of light penetration in proportion to depths were as follows; clear : $57.90\% (5m),\;23.40\%\;(15m),\;6.23\%\;(30m),\;1.00\%\;(50m).$ $red\;:\;48.95\%\;(5m),\;14,81\%\;(15m),\;2.76\%\;(30m),\;0.28\%\;(50m).$ $green:\;63.20\%\;(5m),\;30.47\%\;(15m),\;10.03\%\;(30m),\;2.24\%\;(50m).$ $blue\;:\;62.70\%\;(5m),\;30.00\%\;(15m),\;9.75\%\;(30m),\;1.70\%\;(50m)$

• Childhood Kidney Diseases
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• v.10 no.1
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• pp.8-17
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• 2006

Purpose : We describe the changes of rat glomerular epithelial cells when exposed to high levels of glucose and advanced glycosylation endproducts(AGE) in the in vitro diabetic condition. We expect morphological alteration of glomerular epithelial cells and permeability changes experimentally and we may correlate the results with a mechanism of proteinuria in DM. Methods : We made 0.2 M glucose-6-phsphate solution mixed with PBS(pH 7.4) containing 50 mg/mL BSA and pretense inhibitor for preparation of AGE. As control, we used BSA. We manufactured and symbolized five culture dishes as follows; B5 - normal glucose(5 mM) + BSA, B30 - high glucose(30 mM) + BSA, A5 - normal glucose(5 mM) + AGE, A30 - high glucose(30 mM) + AGE, A/B 25 - normal glucose(5 mM) + 25 mM of mannitol(osmotic control). After the incubation period of both two days and seven days, we measured the amount of heparan sulfate proteoglycan(HSPG) in each dish by ELISA and compared them with the B5 dish at 2nd and 7th incubation days. We observed the morphological changes of epithelial cells in each culture dish using scanning electron microscopy(SEM). We tried the permeability assay of glomerular epithelial cells using cellulose semi-permeable membrane measuring the amount of filtered BSA through the apical chamber for 2 hours by sandwich ELISA. Results : On the 2nd incubation day, there was no significant difference in the amount of HSPG between the 5 culture dishes. But on the 7th incubation day, the amount of HSPG increased by 10% compared with the B5 dish on the 2nd day except the A30 dish(P<0.05). Compared with the B5 dish on the 7th day the amount of HSPG in A30 and B30 dish decreased to 77.8% and 95.3% of baseline, respectively(P>0.05). In the osmotic control group (A/B 25) no significant correlation was observed. On the SEM, we could see the separated intercellular junction and fused microvilli of glomerular epithelial cells in the culture dishes where AGE was added. The permeability of BSA increased by 19% only in the A30 dish on the 7th day compared with B5 dish on the 7th day in the permeability assay(P<0.05). Conclusion: We observed not only the role of a high level of glucose and AGE in decreasing the production of HSPG of glomerular epithelial cells in vitro, but also their additive effect. However, the role of AGE is greater than that of glucose. These results seems to correlate with the defects in charge selective barrier. Morphological changes of the disruption of intercellular junction and fused microvilli of glomerular epithelial cells seem to correlate with the defects in size-selective barrier. Therefore, we can explain the increased permeability of glomerular epithelial units in the in vitro diabetic condition.

• Childhood Kidney Diseases
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• v.9 no.2
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• pp.119-127
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• 2005

Purpose : Podocytes are critical in maintaining the filtration barrier of the glomerulus and are dependent on the integrity of slit diaphragm(SD) proteins including nephrin, p-cadherin, and others. Diabetic proteinuric condition demonstrates defects in SD molecules as well as ultrastructural changes in podocytes. We examined the molecular basis for this alteration of SD molecules especially on P-cadherin as a candidate regulating the modulation of pathogenic changes in the barrier to protein filtration. Methods : To investigate whether high glucose and AGE induce changes in SD, we cultured rat GEpC under normal(5 mM) or high glucose(30 mM) and AGE- or BSA-added conditions and measured the change of P-cadherin expression by Western blotting and RT-PCR. Results : We found that administration of high glucose decreased the P-cadherin production significantly in the presence or absence of AGE by Western blotting. In RT-PCR high glucose with or without AGE also significantly decreased the expression of P-cadherin mRNA compared to those of controls. Such changes were not seen in the osmotic control. Conclusion : We suggest that high glucose with or without AGE suppresses the Production of P-cadherin at the transcriptional level and that these changes nay explain the functional changes of SD in diabetic conditions. (J Korean Soc Pediatr Nephrol 2005;9:119-127)

• The Korean Journal of Food And Nutrition
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• v.10 no.4
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• pp.475-479
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• 1997

The effects of amino acids and metabolites in growth media on the biosynthesis of prodigiosin from Serratia marcescens ATCC 25419 were examined. The prodigiosin synthesis was decreased approximately by 50 to 80% by several amino acids and metabolites tested. The prodigiosin synthesis was increased approximately by 20 to 40% by a low concentration of glyoxylate(1 to 3mM) and outstandingly increased by 122% at 5mM concentration under anaerobic condition. However, the prodigiosin synthesis was decreased approximately by 50 to 90% at a high concentration(20 to 30mM) under anaerobic condition. The prodigiosin was not synthesized by pyruvate and $\alpha$-ketobutyrate under aerobic and anaerobic condition, with addition to glyoxylate under aerobic condition, among the range from 0.5 to 30mM, while the cell growth under anaerobic condition was decreased distinctly by a high concentration(20mM above) of glyoxylate. These data suggest that the growth and prodigiosin of S. marcescens is positively regulated by a low concentration of glyoxylate (1-5mM), but repressed by a high concentration of glyoxylate(20mM above) unlike pyruvate and $\alpha$-ketobutyrate.

• Journal of Life Science
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• v.8 no.2
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• pp.197-202
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• 1998

The cell of Kluyveromyces fragilis yeast, which is worthy of an algal substitute, was disrupted by a chemical treatment to increase the digestion of filter-feeders that yeasts are fed to. The optimum conditions of the chemical treatment were obtained by incubating yeasts at 3$0^{\circ}C$ for one hour after treated by 1 M of Na$_{2}$-EDTA that was dissolved in 0.2 M of Tris-buffer and by 0.3 m of 2-mercaptoethanol. The percentage of protop[last production was about 30%. The percentage could be doubled by the pretreatment of three times of 30 seconds sonication.

• KSBB Journal
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• v.9 no.2
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• pp.191-199
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• 1994

Protoplasts of both strains were produced by lysozyme digestion at $30^{\circ}C$ for 180min. Both strains were treated with $40{\mu}g$/ml of lysozyme in 30mM Tris-HCl buffer(pH 7.5) containing 10% sucrose at the late logarithmic growth phase. It was found that the efficiency of protoplast formation was high at $30^{\circ}C$ and pH 7.5 by measuring the decrease in absorbance. Optimum concentrations of sucrose $Ca^{2+}, \;Mg^{2+}$ for protoplast formation were determined to be 15%, 20mM and 6mM, respectively. Hydrolysis of cell wall and protoplast formation efficiency for L. plantarum showed better results than those for Leu. mesenteroides. The resistances to antibiotics erythromycin and chloramphenicols were chosen as the selection marker for the fusant between L. plantarum and Leu. mesenteroides. Production phase of protoplast in Leu. mesenteroides was also compared with L. plantarum in this paper.

• Korean Journal of Soil Science and Fertilizer
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• v.30 no.3
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• pp.265-271
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• 1997

The salt accumulation, and chemical properties of 90 samples of the plastic film house soil in the area of Cheongju and Chungju were surveyed. Soil textural distribution of soil samples was 30% for sandy loam, 27% for loam and 43% for silty loam. Percentage distribution of electrical conductivity(EC) of surface soil was 23% below $2dS\;m^{-1}$, 30% for $2{\sim}4dS\;m^{-1}$, 25% for $4{\sim}6dS\;m^{-1}$ and 22% over $6dS\;m^{-1}$. Salt affected soil, which EC was higher than $4dS\;m^{-1}$, covered nearly 50% of all field surveyed. However subsoils(20~30cm) below $2dS\;m^{-1}$ was 68%. Salts in plastic film house soil was accumulated by increasing the cultivation period. After 5 years of cultivation electrical conductivity in plastic house soil was generally higher than $4.47dS\;m^{-1}$ in EC that was 2.8~5.6 times higher than that in the field soil in the outside of plastic film house. As the result of temporary removal of plastic film cover from the house during the rainy summer season, salt content in soil was decreased from $3.54{\sim}7.36dS\;m^{-1}$ to $0.71{\sim}2.92dS\;m^{-1}$ in EC due to the desalinization by runoff and percolating water. Contents of $NO_3-N$, $SO_4-S$ and Cl in plastic film house soil were 2.5. 7.0 and 3.4 times higher than those of open field respectively.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.36 no.1
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• pp.17-24
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• 2018

DEM (Digital Elevation Model) is a useful dataset which represents the earth surface. Beside many applications, production and frequent update of DEM is a costly task. Recently global satellite based DEMs are available which has huge potential for application. To check the accuracy, this study compares two global DEMs: AW3D30 (Advanced Land Observing Satellite World 3D 30m) and SRTM30 (Shuttle Radar Topography Mission Global 30m) with reference resampled LiDAR DEM 30m in a test area around Chuncheon, Korea. The comparison analysis was based on statistics of each DEM, their difference, profiles, slope, basin and stream orders. As a result, it is found that SRTM30 and AW3D30 were much similar but inconsistent in the test area compared to the LiDAR30 DEM. In addition, SRTM30 shows less difference with LiDAR30 compared to the AW3D30 DEM. But, DEMs should be very carefully examined for area which has temporal or season changes. For basin and stream analysis, global DEMs can be used only for regional scale analysis not local large scales.