• Molecules and Cells
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• v.20 no.2
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• pp.201-209
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• 2005

We have constructed an AFLP-based linkage map of Japanese red pine (Pinus densiflora Siebold et Zucc.) using haploid DNA samples of 96 megagametophytes from a single maternal tree, selection clone Kyungbuk 4. Twenty-eight primer pairs generated a total of 5,780 AFLP fragments. Five hundreds and thirteen fragments were verified as genetic markers with two alleles by their Mendelian segregation. At the linkage criteria LOD 4.0 and maximum recombination fraction 0.25(${\theta}$), a total of 152 markers constituted 25 framework maps for 19 major linkage groups. The maps spanned a total length of 2,341 cM with an average framework marker spacing of 18.4 cM. The estimated genome size was 2,662 cM. With an assumption of equal marker density, 82.2% of the estimated genome would be within 10 cM of one of the 230 linked markers, and 68.1% would be within 10 cM of one of the 152 framework markers. We evaluated map completeness in terms of LOD value, marker density, genome length, and map coverage. The resulting map will provide crucial information for future genomic studies of the Japanese red pine, in particular for QTL mapping of economically important breeding target traits.

• Nuclear Engineering and Technology
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• v.52 no.6
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• pp.1266-1270
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• 2020

A code is developed to correct for the coincidence summing effect in detecting a voluminous gamma source, and this code is applied to a¹⁵²Eu standard source as a test case. The source is 1000 mL of liquid in a cylindrical shape. To calculate the coincidence summing effect, the cylindrical source is considered as 10(radial) × 8(height) sectional sources. For each sectional source, the peak efficiency and total efficiency are obtained by Monte Carlo simulation at each energy for 10 energies between 50 keV and 2000 keV. The efficiencies of each sector are then expressed as polynomials of gamma energy. To calculate the correction coefficients for the coincidence summing effect, the KORSUM code is used after modification. The magnitudes of correction are 4%-17% for the standard ¹⁵²Eu source measured in this study. The relative deviation of 4.7% before the coincidence correction is reduced to 0.8% after the correction is applied to the efficiency based on the measured gamma line. Hence, this study has shown that a new method has been developed that is applicable for correcting the coincidence effect in a voluminous source, and the method is applied to the measured data of a standard ¹⁵²Eu cylinder source.

• Proceedings of the PSK Conference
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• 2000.10a
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• pp.152.2-153
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• 2000

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.25 no.12
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• pp.1236-1242
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• 2014

In this work, a D-band VCO(Voltage Controlled Oscillator) has been developed in a 65-nm CMOS technology. The circuit was designed based on push-push mechanism. The output oscillation frequency of the fabricated VCO varied from 152.7 GHz to 165.8 GHz, and the measured output power was from -17.3 dBm to -8.7 dBm. A phase noise of -90.9 dBc/Hz is achieved at 10 MHz offset. The chip size of the circuit is $470{\mu}m{\times}360{\mu}m$ including the probing pads.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.152-152
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• 2017

Plants exposed to environmental stress for long durations often can adapt to stress conditions with improved tolerance. Moreover this acquired tolerance to stress can be retained even after reverting to destressed growth conditions, which is known to stress memory. In these adaptation and stress memory processes, epigenetic regulation, such as DNA methylation and histone modifications play a key role. Here, we showed that regenerated rice plants from embryogenic callus exposed to gradually increasing NaCl concentrations (up to 120 mM NaCl) acquired salt tolerance and their enhanced tolerance are inherited to subsequent generations. The rice plants (R0) regenerated from rice callus under saline conditions were transplanted into normal paddy field and R1 seeds were harvested. These R1 seeds displayed higher germination rate on MS medium containing 100mM NaCl than wild-type. The callus derived from R1 seeds showed better growth than control callus on high salinity medium. And the salt-adapted R1 plants exhibited higher chlorophyll contents and also higher $K^+/Na^+$ ratio than wild-type rice under saline conditions. The results indicated that rice plants successfully adapted to saline growth conditions during regeneration on high salt medium and moreover this acquired tolerance to salt stress was inherited subsequent generation.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2012.10a
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• pp.151-152
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• 2012

• Proceedings of the Korea Water Resources Association Conference
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• 2005.09a
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• pp.152-153
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• 2005

• Procedding of Korea Feed Ingredients Association Conference
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• v.2
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• pp.147-152
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• 2001

• Electrical & Electronic Materials
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• v.11 no.2
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• pp.149-152
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• 1998

• Korean Journal of Environmental Biology
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• v.35 no.2
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• pp.152-159
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• 2017

For the effective seedling production of the hard shelled mussel, Mytilus coruscus, this study assessed the effects of the dietary value of live food, density, water temperature and salinity on growth and survival rate of the larvae. The optimal survival rate and growth rate were examined under differing conditions of water temperature, salinity, and rearing density for 30 days. The three groups were provided different feeding organisms, such as Isochrysis galbana and Teleaulax suecica. The mixtures were provided at a rate of $5{\times}10^4cell\;mL^{-1}$. The best growth was observed in the group with conditions $21^{\circ}C$ water temperature ($16.2{\pm}9.1{\mu}m$), 33 psu of salinity ($16.82{\pm}3.9{\mu}m$), $2500individual\;m^{-2}$ ($17.2{\pm}5.9{\mu}m$), and fed with $5{\times}10^4cell\;mL^{-1}$ of I. galbana and T. suecica mixture ($16.0{\pm}7.3{\mu}m$). The highest survival rate was found in the group at conditions $18^{\circ}C$ water temperature (66.4%), 33 psu of salinity (24.4%), $2500individual\;m^{-2}$ (65.8%), and fed with $5{\times}10^4cell\;mL^{-1}$ of I. galbana and T. suecica mixture (58.8%). We therefore conclude that the suitable culture conditions for the stable production of hard shelled mussel artificial seedlings was at 18 to $21^{\circ}C$ of temperature, 30 to 33 psu of salinity, 2500 to $5000individual\;m^{-2}$ of rearing density, and feeding supplement of $5{\times}10^4cell\;mL^{-1}$ of I. galbana and T. suecica mixture under semi running water system.