• Title/Summary/Keyword: M152

Search Result 811, Processing Time 0.027 seconds

Development of Novel Method for the Detection of Microcystin Using Chemiluminescence Immunochromatography

  • Pyo, Dong-Jin;Yoo, Ji-Sun
    • Bulletin of the Korean Chemical Society
    • /
    • v.32 no.1
    • /
    • pp.149-152
    • /
    • 2011
  • A new chemiluminescence immunochromatographic analysis system with high sensitivity and high reproducibility was developed for the determination of microcystins (MCs) in water. Horse radish peroxidase (HRP) labeled microcystin monoclonal antibody was used for the sensitive chemiluminescence detection. The chemiluminescence immunochromatographic analysis system was composed of microcystin LR (MCLR)-monoclonal antibody (mAb)-Horse Radish Peroxidase (HRP) conjugate, MCLR-BSA conjugate, luminol, hydrogen peroxide mixture solution, an immunochromatographic assay strip and luminometer. To detect the concentration of microcystins in water, we utilized one spot analysis of the strip instead of flow type analysis. We could detect the microcystins in water at a concentration as low as 9.45 pg/mL with the chemiluminescence (CL) detection.

Survey on Potato Parasitic Nematodes (감자 증산을 위한 기생선충 조사)

  • Choi Young-Euon;Choi Dong-Ro
    • Korean journal of applied entomology
    • /
    • v.21 no.3 s.52
    • /
    • pp.146-152
    • /
    • 1982
  • Survey on potato parasitic nematodes has been undertaken in order to find distribution of the nemic fauna. 41 soil samples were taken from potato fields in Gyeongbug, Gyeongnam and Gangweon provinces. Twenty four species belonging to 16 different genera were identified. Potato cyst nematode, Globodera rostochiensis was not found in the areas. Potato-rot nematode, Ditylenchus destructor and stem nematode, Ditylenchus dipsaci were found from several potato fields and population density high and showed damage to the crops. Root-lesion nematode, Pratylenchus minyus, Pratylenchus penetrans, Pratytenchus thornei and Pratylenchus vulnus were found and their population of these four species were high depending on the fields. Root-knot nematode, Meloidogyne hapla and Meloidogyne incognita were found. M. hapla was found only in Gangweon province and the population density was high. M. incognita was found at Milyang in Gyeongnam province. Spiral nematode, Heticotylenchus digonichus, Helicotylenchus dihystera, Helicotylenchus pseudorobustus, Rotylenchus orientalis and Rotylenchus pini were found. Aphelenchoides saprophilus, Criconemoides informis, Ditylenchus destructor, D. dipsaci, Helicotylenchus digonichus, H. dihystera, Hemicriconemoides intermedius, Meloidogyne hapla, Psilenchus hilarulus, Pratylenchus minyus, and Xiphinema americanum were first found from potato fields in Korea.

  • PDF

A New Correlation on Heat Transfer Coefficient in Horizontal Multi Channels (수평 다채널에서의 열전달 계수에 관한 새로운 상관식)

  • CHOI, Yong-Seok;LIM, Tae-Woo
    • Journal of Fisheries and Marine Sciences Education
    • /
    • v.28 no.5
    • /
    • pp.1388-1394
    • /
    • 2016
  • This paper presents a experimental study of two-phase flow boiling of FC-72 in multi channels. Flow boiling heat transfer coefficients are obtained with mass flux ranging from 152.9 to $353.9kg/m^2s$ and heat flux from 5.6 to $46.1kW/m^2$. The experimental results show that the heat transfer is governed by nucleate boiling mechanism in the low heat flux region. However, it is found that the effects of nucleate boiling and forced convection boiling are combined as the heat flux increases. A new correlation to predict the heat transfer coefficient is developed by using the dimensionless number such as Reynolds number, Weber number, boiling number. This correlation shows good predictive accuracy against the measured data.

Synthesis of 4-(2-thiazolyloxy)phenylalkanoic Acids As An An-tiinflammatory Agent

  • Choi, Hong-Dae;Shin, Sang-Hoon;Son, Byeng-Wha
    • Archives of Pharmacal Research
    • /
    • v.19 no.2
    • /
    • pp.148-152
    • /
    • 1996
  • The efficient synthesis of 4-(2-thiazolyloxy)phenylalkanoic acids (10a-c), which are a potent antiinflammatory agent, was achieved in 5-6 steps starting from isopropoxybenzene and methyl $\alpha-chloro-\alpha-(methylthio)acetate (1)$. The key intermediate (4) was prepared by Friedel-Crafts reaction of isopropoxybenzene with (1) followed by desulfurization and the removal of isopropyl protector. Methyl 4-hydroxyphenylalkanoates (6, 8) were similarly obtained from alkylation of (3) and deprotection.

  • PDF

Tests of Slab-Column Connections with Partially Debonded Reinforcement under Cyclic Lateral Loading (부분적인 비 부착 철근을 갖는 슬래브-기둥 접합부의 반복 횡하중 실험)

  • Choi, Jung-Wook;Song, Jin-Kyu
    • Proceedings of the Korea Concrete Institute Conference
    • /
    • 2006.11a
    • /
    • pp.13-16
    • /
    • 2006
  • This article summarizes tests conducted on two full-scale interior slab-column connections with and without partially debonded reinforcement subjected to cyclic loading. Each test specimen consisted of a 4.2m square slab with a 355mm square column protruding 1.5m above and below the slab. The slab thickness was 152mm. The specimen with partially debonded reinforcement exhibited more lateral drift capacity than did the specimen with fully bonded reinforcement. With partial debonding of the flexural reinforcement, cyclic load appeared to produce less damage to the connection in the vicinity of the slab-column joint region.

  • PDF

A Study of the Characteristics and Mechanism of Giant wave Appearance (대양에서의 거대파랑 출현 특성과 기구에 관한 연구)

  • Shin Seung-Ho;Hong Keyyong
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
    • /
    • 2005.10a
    • /
    • pp.147-152
    • /
    • 2005
  • 선형파 이론에 의한 파랑스펙트럼 분포에 의해서는 30m 크기의 파랑은 현실적으로 거의 발생 불가능하다고 인식되어 왔다. 그러나 최근의 위성 영상을 이용한 조사에 의해 3주간의 기간 통안 25m 이상의 거대파가 10개 이상 관측됨에 따라 실해역에서 빈번히 마주칠 수 있는 현상임이 입증되었으며 이에 따라 지금까지 이유 불명으로 치부되어 왔던 많은 해양 재난이 거대파에 의해 발생했던 것으로 추정되고 있다. 거대파의 발생원인은 파군 형성과 관련한 파고분포 특성의 변화, 전파하는 파군의 비선형 공명간섭 통이 제기되고 있으나, 그 출현의 복잡성과 자료의 부족 등으로 아직 명확하게 해명되지 못하고 있다. 본 연구에서는 실해역에서 발생하는 거대파의 특성 및 선형 및 비선형이론에 근거한 거대파 발생 기구를 고찰하고 비선형 파랑전파를 모사할 수 있는 수치모형을 개발하였다.

  • PDF

Isolation of a petunia cell growth inhibitor from Streptomyces sp. 9602 (Streptomyces sp. 9602 균주로부터 페튜니아 캘러스 생장억제물질의 분리)

  • 김명조;곽상수
    • Korean Journal of Plant Tissue Culture
    • /
    • v.24 no.3
    • /
    • pp.149-152
    • /
    • 1997
  • To search for a compound inhibiting the petunia callus growth from Streptomyces sp., we investigated the activity in the culture broth of 400 strains. The active compound was successively purified with solvent fractionation, silica gel column chromatography from Streptomyces sp. 9602 strain, and identified as 2, 5, 7-trihydroxy-3-(5'-hydroxyhexyl)-1, 4-naphthoquinone by 1H-NMR, EI-MS, IR and UV. It inhibited the callus growth of petunia by 50% at $32\mu\textrm{g}$/mL.

  • PDF

Probabilistic Simulation for Extraction of Reliability Design Data (설계자료 추출을 위한 확률 시뮬레이션)

  • 김선진
    • Journal of the Korean Society of Fisheries and Ocean Technology
    • /
    • v.29 no.2
    • /
    • pp.152-161
    • /
    • 1993
  • This paper deals with the effect of spatial distribution of material properties on its statistical characteristics and numerical estimation method of reliability of fatigue sensitive structures with respect to the fatigue crack growth. A method is proposed to determine experimentally the probability distribution functions of material parameters of Paris law. da/dN=C(ΔK/K sub(0) ) super(m), using stress intensity factor controlled fatigue tests. The result with a high tensile strength steel shows that the distribution of the parameter m is approximately normal and that of 1/C, is a 3-parameter Weibull. The main result obtained are : (1) The theoretical autocorrelation of the resistance, 1/C, to fatigue crack growth are almost same for different lengths. (2) The variance decreases with the increasing a averaging length. When spatial correlation length is very small. the variane decreases significantly were the averaging length. (3) The probability distribution of load cycles or the number for a crack to reach a certain length can be estimated using these functions by simulation of non-Gaussian(expecially Weibull) Stochastic Process.

  • PDF

Partial Purification and Characterization of a Soluble $\beta$-Fructoguracosidase from Onion (Allium cepa)

  • Lee, Yong-Eok;Yoo, Jin-Young
    • Preventive Nutrition and Food Science
    • /
    • v.3 no.2
    • /
    • pp.152-156
    • /
    • 1998
  • A Fructan-degrading enzyme was partially purified from onion (Allium cepa)bulbs by a combination of ammonium sufate precipitation, concanavalin-A-Affinity chromatography, and ion-exchange and gel-filtration chromatography. The enzyme hydrolyzed sucrose more effectively than inulin and was identified as a $\beta$- fructofuranosidase (invertase). The optimum pH and temperature were pH 5.5 and 35$^{\circ}C$, respectively. The enzymehydrolyzed sucrose with a Km of 1.2mM . The soluble $\beta$-fructofuranosidase is likely glycoprotein based on its ability to bind the lectin concanavalin-A. The enzyme was heatlabie, with mose activity being lost at 5$0^{\circ}C$ in 1 hr of incubation. The onion $\beta$-fructofuranosidase was partially inhibited by ZnCl2 HgCl2 and CuSo4.

  • PDF

ON THE BOUNDS OF THE EIGENVALUES OF MATRIX POLYNOMIALS

  • Wali Mohammad Shah;Zahid Bashir Monga
    • Korean Journal of Mathematics
    • /
    • v.31 no.2
    • /
    • pp.145-152
    • /
    • 2023
  • Let $P(z):=\sum\limits^{n}_{j=0}A_jz^j$, Aj ∈ ℂm×m, 0 ≤ j ≤ n be a matrix polynomial of degree n, such that An ≥ An-1 ≥ . . . ≥ A0 ≥ 0, An > 0. Then the eigenvalues of P(z) lie in the closed unit disk. This theorem proved by Dirr and Wimmer [IEEE Trans. Automat. Control 52(2007), 2151-2153] is infact a matrix extension of a famous and elegant result on the distribution of zeros of polynomials known as Eneström-Kakeya theorem. In this paper, we prove a more general result which inter alia includes the above result as a special case. We also prove an improvement of a result due to Lê, Du, Nguyên [Oper. Matrices, 13(2019), 937-954] besides a matrix extention of a result proved by Mohammad [Amer. Math. Monthly, vol.74, No.3, March 1967].