• Current Optics and Photonics
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• 제1권1호
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• pp.12-16
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• 2017

Color sensor systems based on M13 bacteriophage are being considerably researched. Although many studies on M13 bacteriophage-based chemical sensing of TNT, endocrine disrupting chemicals, and antibiotics have been undertaken, the fundamental physical and chemical properties of M13 bacteriophage-based nanostructures require further research. A simple M13 bacteriophage-based colorimetric sensor was fabricated by a simple pulling technique, and M13 bacteriophage was genetically engineered using a phage display technique to exhibit a negatively charged surface. Arrays of structurally and genetically modified M13 bacteriophage that can determine the polarity indexes of various alcohols were found. In this research, an M13 bacteriophage-based color sensor was used to detect various types of alcohols, including methanol, ethanol, and methanol/butanol mixtures, in order to investigate the polarity-related property of the sensor. Studies of the fundamental chemical sensing properties of M13 bacteriophage-based nanostructures should result in wider applications of M13 bacteriophage-based colorimetric sensors.

• Journal of Microbiology
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• 제42권3호
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• pp.228-232
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• 2004

In this paper, we investigated the development of a simplified and rapid primary capture step for the recovery of M13 bacteriophage from particulate-containing feedstock. M13 bacteriophage, carrying an insert, was propagated and subsequently purified by the application of both conventional multiple steps and expanded bed anion exchange chromatography. In the conventional method, precipitation was conducted with PEG/NaCl, and centrifugation was also performed. In the single step expanded bed anion exchange adsorption, UpFront FastLine$\_$TM/20 (20mm i.d.) from UpFront Chromatography was used as the contactor, while 54$m\ell$ (H$\_$o/=15cm) of STREAMLINE DEAE (p=1.2 g/㎤) from Amersham Pharmacia Biotechnology was used as the anion exchanger. The performance of the two methods were evaluated, analysed, and compared. It was demonstrated that the purification of the M13 bacteriophage, using expanded bed anion exchange adsorption, yielded the higher recovery percentage, at 82.86％. The conventional multiple step method yielded the lower recovery percentage, 36.07％. The generic application of this integrated technique has also been assessed.

• 미생물학회지
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• 제26권3호
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• pp.162-166
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• 1988

Bacteriophage M13 DNAs carrying the wild type or base substituted SV40 DNA replication origins were used for replication assay. In vivo and in vitro assay with African green monkey cell line COS-1 showed that the replication of M13-SV40 recombinant DNAs was restricted like a pBR322 SV40 recombinant DNA(Lusky and Botchan, 1981). Furthermore, recombinant phage DNAs isolated from the transfected siminan cells subsequently show a reduced ability to retransform E. coli. But pATSV-W(Kim et al., 1988) was replicated in COS-1 cells normally. We think that a poison sequence may exist on bacteriophage M13 DNA like pBR322.

• Toxicological Research
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• 제5권1호
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• pp.53-59
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• 1989

A mycotoxin Patulin, isolated from apple juice medium cultured with Penicillium patulum NRRL5259, was purified through acid aluminum column using ethyl ether as eluent. The yield of purified patulin crystal was 3mg/ml culture medium after 8 days of shaking culture at 28C. The growth rate of Escherichia coli K12JM103 infected with bacteriophage M13 was decreased by patulin at the concentration range of 1Mug/ml to 10Mu/nl. ED50 of patulin for the bacterial growth was 4.5Mug/ml and 10Mug/ml of patulin caused maximum inhibitory effect (60% inhibition) on the growth.

• 미생물과산업
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• 제11권1호
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• pp.13-20
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• 1985

특이 단백질(gene II protein)의 정성적 또는 정량적 변화에 의해 DNA 복제에 필요한 replication orgin sequence의 일부분이었단 replication enhancer를 불필요하게 함으로써 minimum replication origin을 core region만으로 국한 되게 함이었다.

• 한국고분자학회지:고분자과학과기술
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• 제25권4호
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• pp.307-314
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• 2014

• Journal of Microbiology
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• 제37권2호
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• pp.97-101
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• 1999

Random hexapeptide library on the surface of filamentous bacteriophage was constructed using the SurfZAP vector. The size of the library was approximately 105. The peptide insert was flanked by two cysteines to constrain the peptide structure with a disulfide bond. This library was screened for the topoisomerase II binding peptide. Dramatic enrichment of the fusion phage over the VCS M13 helper phage was demonstrated by bio-panning affinity selection.

• 한국가금학회지
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• 제44권3호
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• pp.181-188
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• 2017

박테리오파지는 세균에 특이적으로 감염하는 바이러스로 용해 능력이 있는 박테리오파지는 동물질병을 치료하는 항생제 대체제로 사용할 수 있다. 본 연구는 닭에서 가금티푸스를 유발하는 Salmonella enterica serovar Gallinarum(Salmonella Gallinarum)에 대한 박테리오파지를 분리하고, 그 특성을 구명하고자 하였다. 7개의 닭 도축장 하수 처리장 물로부터 10주의 박테리오파지를 분리하였다. 이중 $SG{\Phi}-YS$ SP로 명명된 파지는 전자현미경 관찰 결과, Myoviridae family로 분류되었다. 분리한 박테리오파지의 숙주 범위를 확인하기 위하여 Salmonella Gallinarum 7주와 Salmonella Enteritidis 5주에 대한 용해 유발능력을 조사한 결과, $SG{\Phi}-YS$ SP는 Salmonella Gallinarum만 용해하는 특징을 보였다. $SG{\Phi}-YS$ SP는 Salmonella Gallinarum에 접종하였을 경우 6시간과 9시간 뒤 각각 2.21 log cfu/mL 및 2.13 log cfu/mL를 감소시키는 것으로 확인되어 닭에서 Salmonella Gallinarum 감염을 예방하거나 치료하는 파지 제제 개발에 이용될 수 있을 것으로 판단되었다.

• 한국식품위생안전성학회지
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• 제35권2호
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• pp.189-194
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• 2020

본 연구는 항생제 내성 Salmonella Typhimurium CCARM 8009을 저해하기 위한 phage와 항생제 조합처리의 효과를 평가하였다. 디스크 확산법과 액체배지 희석법에 의해 phage와 항생제의 상승 저해효과를 측정하였고 배양을 통한 항생제 내성 유도를 평가하였다. Phage를 처리한 cefotaxime, chloramphenicol, ciprofloxacin, erythromycin의 디스크의 저해 구역은 각각 13.6%, 19.3%, 12.7%, 78.8%로 증가되었다. Phage와 항생제 조합 처리에 의해 tetracycline, chloramphenicol, ciprofloxacin, erythromycin, streptomycin의 최소생육억제농도는 각각 64, 4, 0.0078, 64, 256 mg/mL으로 감소되었다. Phage와 항생제의 조합 처리는 항생제 내성 S. Typhimurium CCARM 8009을 효과적으로 저해하였다 (4 log reduction). 본 결과는 phage와 항생제의 조합처리는 항생제 내성균을 제어하기 위한 방법으로 충분히 응용가치가 높음을 보여주고 있다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2013년도 제45회 하계 정기학술대회 초록집
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• pp.90.1-90.1
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• 2013

Nature utilizes various of the colorization process. Some species of birds can express their mood of tempers by changing their collagen structures on skin. For example, turkey can change their skin color by expansion of the collagen structures, which are associated with the distinct color changes. Here, we developed bioinspired virus-based colorimetric sensors which can be genetically tuned for target molecule. Using M 13 bacteriophage, we fabricated responsive self-assembled color matrices composed of quasi-ordered fiber bundle structures. These virus matrices can exhibit color change by stimuli through fiber bundle structure modulation. Upon exposure of volatile organic compounds, the resulting multi-colored matrices exhibited distinct color changes with different ratios that can be recognized by the naked eyes. Using the directed evolutionary approaches, we genetically engineered the virus matrix to incorporate binding motif for explosive detection (i.e., trinitrotoluene (TNT)). Through utilizing a common handheld device (i.e., iPhone), we could distinguish TNT molecules down to 20 ppb in a selective manner. Our novel biomimetic virus colorimetric sensor can overcome current limitation for low response selectivity.