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http://dx.doi.org/10.5536/KJPS.2017.44.3.181

Characterization of Bacteriophages against Salmonella Gallinarum  

Kim, Minjeong (College of Veterinary Medicine, Kangwon National University)
Kwon, Hyuk-Moo (College of Veterinary Medicine, Kangwon National University)
Sung, Haan-Woo (College of Veterinary Medicine, Kangwon National University)
Publication Information
Korean Journal of Poultry Science / v.44, no.3, 2017 , pp. 181-188 More about this Journal
Abstract
Bacteriophages are viruses that exclusively infect bacterial cells, and lytic bacteriophages can be used as a safe alternative to antibiotics for the prevention and treatment of animal diseases. In this study, we attempted to isolate and characterize bacteriophages for Salmonella enterica serovar Gallinarum (Salmonella Gallinarum), the causative agent of fowl typhoid in chickens. Ten bacteriophages were isolated from samples of sewage from seven poultry slaughterhouses. One of these isolate, designated as $SG{\Phi}-YS$ SP and classified in the family Myoviridae, produced plaques with seven Salmonella Gallinarum strains. However, no plaques were produced with any of the Salmonella enterica serovar Enteritidis strains tested, suggesting that this bacteriophage is Salmonella Gallinarum specific. To assess the lytic ability of $SG{\Phi}-YS$ SP against Salmonella Gallinarum, bacterial growth rates following inoculation of the bacteriophage were compared with the control. The $SG{\Phi}-YS$ SP treatment, with a multiplicity of infection of 10, reduced the growth of Salmonella Gallinarum by 2.21 log cfu/mL at 6 h, and 2.13 log cfu/mL at 9 h, suggesting that this bacteriophage isolate could be used for the prevention or treatment of Salmonella Gallinarum infection in chickens.
Keywords
bacteriophage; Salmonella Gallinarum; lytic effect; chicken;
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