• 제목/요약/키워드: M. globosa

검색결과 17건 처리시간 0.069초

각질형세포와 피지선세포 공배양을 통한 지루성 피부염 억제 소재 연구 (The Study about Relief Effect of Essential Oil on Seborrheic Dermatitis with Co-culture System)

  • 김아름;김수나;이현기;전병배;박원석
    • 대한화장품학회지
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    • 제38권4호
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    • pp.311-319
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    • 2012
  • 보통 지루성 피부염은 과다 생성된 피지와 함께 피지를 영양분으로 상재하는 M. globosa 이스트에 의해서 얼굴, 몸 및 두피에 자주 발생하는 피부질환이다. 본 연구는 천연정유성분(Natural essential oils)으로부터 지루성 피부염과 관련된 항염증 활성과 M. globosa 이스트에 대한 증식 억제 활성을 평가하고자 하였다. 지루성 피부염 진정 효능을 평가하기 위해서 지루성 피부염 환경을 모사한 공배양 세포 평가 모델을 구축하였다. 본 평가모델을 통해서 육계(Cinnamomum zeylanicum, Cinnamon bark)의 껍질 및 박하(Mentha arvensis, Cornmint)의 잎에서 추출한 천연정유 성분이 다양한 지루성 피부염 자극원에 따른 Interleukin-$1{\alpha}$ (IL-$1{\alpha}$), Interleukin-6 (IL-6), Interleukin-8 (IL-8) 사이토카인의 발현량 증가와 피지생성 증가를 감소시킴을 확인하였다. 또한, 지루성 피부염 유발균으로 알려진 M. globosa 이스트에 대한 증식 저해 평가를 통해 두 정유성분에서 유의한 M. globosa 증식 억제 능(MIC ${\leq}0.0625%$)을 확인하였다. 본 연구 결과로부터 육계껍질 및 박하 잎 정유성분이 지루성 피부염에 따른 염증성 사이토카인의 발현량을 감소시켰다. 특히 육계 껍질 오일은 지루성 피부염 주요 원인인 Malassezia furfur 및 M. globosa 증식을 억제함으로써, 지루성 두피 염증의 예방과 효과적인 관리 제품의 소재로서 활용가치가 높음을 알 수 있다.

Cloning and Expression in Pichia pastoris of a New Cytochrome P450 Gene from a Dandruff-causing Malassezia globosa

  • Lee, Eun-Chang;Ohk, Seul-Ong;Suh, Bo-Young;Park, Na-Hee;Kim, Beom-Joon;Kim, Dong-Hak;Chun, Young-Jin
    • Toxicological Research
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    • 제26권1호
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    • pp.47-52
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    • 2010
  • The Malassezia fungi are responsible for various human skin disorders including dandruff and seborrheic dermatitis. Of the Malassezia fungi, Malassezia globosa (M. globosa) is one of the most common in human scalp. The completed genome sequence of M. globosa contains four putative cytochrome P450 genes. To determine the roles of Malassezia P450 enzymes in the biosynthesis of ergosterol, we isolated MGL3996 gene from M. globosa chromosomal DNA by PCR. The MGL3996 gene encodes an enzyme of 616 amino acids, which shows strong similarity with known CYP52s of other species. MGL3996 gene was cloned and expressed in Pichia pastoris (P. pastoris) heterologous yeast expression system. Using the yeast microsomes expressing MGL3996 protein, a typical P450 CO-difference spectrum was shown with absorption maximum at 448 nm. SDS-PAGE analysis revealed a protein band of apparent molecular weight 69 kDa and Western blot with anti-histidine tag antibody showed that MGL3996 was successfully expressed in P. pastoris. Cloning and expression of a new P450 gene is an important step to study the P450 monooxygenase system of M. globosa and to understand the role of P450 enzymes in pathophysiology of dandruff.

Functional Expression and Characterization of Recombinant NADPH-P450 Reductase from Malassezia globosa

  • Lee, Hwa-Youn;Park, Hyoung-Goo;Lim, Young-Ran;Lee, Im-Soon;Kim, Beom-Joon;Seong, Cheul-Hun;Chun, Young-Jin;Kim, Dong-Hak
    • Journal of Microbiology and Biotechnology
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    • 제22권1호
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    • pp.141-146
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    • 2012
  • Malassezia globosa is a common pathogenic fungus that causes skin diseases including dandruff and seborrheic dermatitis in humans. Analysis of its genome identified a gene (MGL_1677) coding for a putative NADPH-P450 reductase (NPR) to support the fungal cytochrome P450 enzymes. The heterologously expressed recombinant M. globosa NPR protein was purified, and its functional features were characterized. The purified protein generated a single band on SDS-PAGE at 80.74 kDa and had an absorption maximum at 452 nm, indicating its possible function as an oxidized flavin cofactor. It evidenced NADPH-dependent reducing activity for cytochrome c or nitroblue tetrazolium. Human P450 1A2 and 2A6 were able to successfully catalyze the O-deethylation of 7-ethoxyresorufin and the 7-hydroxylation of coumarin, respectively, with the support of the purified NPR. These results demonstrate that purified NPR is an orthologous reductase protein that supports cytochrome P450 enzymes in M. globosa.

Skin Commensal Fungus Malassezia and Its Lipases

  • Park, Minji;Park, Sungmin;Jung, Won Hee
    • Journal of Microbiology and Biotechnology
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    • 제31권5호
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    • pp.637-644
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    • 2021
  • Malassezia is the most abundant genus in the fungal microflora found on human skin, and it is associated with various skin diseases. Among the 18 different species of Malassezia that have been identified to date, M. restricta and M. globosa are the most predominant fungal species found on human skin. Several studies have suggested a possible link between Malassezia and skin disorders. However, our knowledge on the physiology and pathogenesis of Malassezia in human body is still limited. Malassezia is unable to synthesize fatty acids; hence, it uptakes external fatty acids as a nutrient source for survival, a characteristic compensated by the secretion of lipases and degradation of sebum to produce and uptake external fatty acids. Although it has been reported that the activity of secreted lipases may contribute to pathogenesis of Malassezia, majority of the data were indirect evidences; therefore, enzymes' role in the pathogenesis of Malassezia infections is still largely unknown. This review focuses on the recent advances on Malassezia in the context of an emerging interest for lipases and summarizes the existing knowledge on Malassezia, diseases associated with the fungus, and the role of the reported lipases in its physiology and pathogenesis.

한국산 감자바이러스병에 관하여 (Studies on potato virus disease in Korea)

  • 박상윤;소인영
    • 미생물학회지
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    • 제3권2호
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    • pp.1-8
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    • 1965
  • The potato viruses, as possible potato virus X(PVX), potato virus Y(PVY), potato virus S(PVS) are isolated from potato tuber, which collected from eleven areas (Table 1) in Korea. These viruses are isolated by single lesion isolation, Aphid transmission and inoculating methods through the many species of the different plants. The PVX is identified by host range, symptoms, physical properties, serological reaction and electron micrography. The other two viruses are identified by the first two methods mentioned above. The results of the above experiments are as follows. The total value of these viruses infection is 81%. The value of PVX infection is higher than the other two viruses. The properties of PVX are marked as local lesions on Comphrena globosa. The dilution end point is $10^{-6}$, the thermal inactivation point is $70^{\circ}C$ and the size of virus particles is around 13 x 60 $m\mu$.

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Characterization of Carnation mottle camovirus(CarMV) Isolated from Lilium spp. in Korea

  • Park, J.H.;J.H. Sung;H.Y. Shin;M.U. Chang;S.N. Yoo
    • 한국식물병리학회:학술대회논문집
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    • 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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    • pp.150.2-150
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    • 2003
  • Carnation mottle carmovirus(CarMV) was isolated from Lilium spp. in Korea. This isolate, CarMV, was done bioassay, which plants were Dianthus caryophyllus, Gomphrena globosa, Chenopodium amaranticolor, Dianths chinensis. CarMV was propagated on the leaves of Chenopodium amaranticolor with the crude-sap inoculation method and purified by Mossops method(1976). We produced antiserum against CarMV and analyzed the antiserum specificity with ELISA, Gel diffusion method, and Rapid Immunofilter Paper Assay (RIPA). From these results of the assay, RIPA method was simple and rapid for CarMV detection. We have established successfully the CarMV detection system. CarMV coat protein gene was amplified by RT-PCR with specific primers and sequencing analysis was done.

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조력발전소 가동 후 시화갯벌의 대형저서동물군집 변화 (Changes in the Macrobenthic Community in Sihwa Tidal Flat After Operation of the Tidal Power Plant)

  • 김민규;구본주
    • Ocean and Polar Research
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    • 제38권4호
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    • pp.315-324
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    • 2016
  • In this study, we investigated changes in the macrobenthic community in Sihwa tidal flat before and after the operation of a Tidal Power Plant (TPP). In order to investigate changes in the macrobenthic community structure, field surveys were conducted at eighteen stations in 4 transect lines every September from 2011 to 2015. Mean density of macrobenthos decreased to $116ind./m^2$ in 2015 from $1,602ind./m^2$ in 2011. While the mean density of macrobenthos has decreased, species richness and biomass have gradually increased. The species diversity and SEP (Shannon-wiener Evenness Proportion) have also gradually increased over time since the operation of the TPP. The macrobenthic community in Sihwa tidal flat was divided into 4 groups on a yearly base. Before the operation of TPP, opportunistic species such as Neanthes succina and Polydora cornuta largely contributed to the structure of the macrobenthic community, while equilibrium species such as Periserrula leucophryna and Scopimera globosa contributed after the operation. With the operation of TPP, the macrobenthic community has rapidly recovered and this might be related to improvement in the quality of water and sedimentary environments as a result of an increase in water mass volume exchanged through the TPP gate.

신두리 해안의 조하대와 모래 갯벌에 서식하는 대형저서동물의 분포 (Distribution of Benthic Macrofauna in subtidal area and on sand flat of Sindu-ri coast, Korea)

  • 구본주;신상호;우한준
    • 한국습지학회지
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    • 제7권2호
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    • pp.69-88
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    • 2005
  • 신두리 해안의 조하대와 갯벌에서 2002년 7월 대형저서동물의 분포를 조사하였다. 조사 해역에서는 총 134종, 3,511개체, 388g의 저서동물이 채집되었다. 조하대 10개 정점에서는 109종, 1,298개체/$m^2$, $69.4g/m^2$의 저서동물이 출현하였으며, 이 가운데 갯지렁이류가 54종, 813개체/$m^2$의 분포를 보이며 출현 종수 (49.5%)와 서식밀도 (62.7%)에서 가장 우점하는 분류군이었다. 집괴분석 결과 신두리 해역에는 퇴적물 특성에 따라 네 개의 저서동물군집이 형성되어 있었다. 펄과 자갈이 혼재하는 양식장 주변 해역에는 Cirrophorus armatus와 Lumbrineris longifolia로 대표되는 군집, 암반기질로 된 해역에는 Aonides oxycephala 군집, 모래 함량이 높으며 최저조선에 인접한 곳에는 Scoloplos armiger와 Grandifoxus cuspis 군집, 그리고 역시 모래 함량이 높으며 외해쪽으로 인접한 해역에는 뚜렷하게 우점하는 종 없이 균등도지수가 높은 군집이 형성되어 있었다. 양식장 주변 해역의 저서동물 군집은 출현 종수, 서식밀도, 종다양도지수가 높았으나 오염지시종들이 우점하고 있었다. 모래 퇴적물 해역에는 S. armiger와 G. cuspis 등이 우점하는 일반적 모래 조하대 군집 특성을 보였다. 갯벌 두 조사선에 설정한 8개 정점에서는 50종, 2,443개체/$m^2$, $381.3g/m^2$의 저서동물이 출현하였으며, 연체동물이 출현 종수, 서식밀도, 생체량에서 각각 20종, 1,345개체/$m^2$, $350.4g/m^2$를 차지하며 가장 우점하는 분류군이었다. Umbonium thomasi, Veneridae sp., Mandibulophoxus mai, Armandia lanceolata, Eohaustorius spinigerus, Urothoe convexa 등이 신두리 갯벌의 우점종이었으며, 전체 개체수의 83% 이상을 이들 종이 차지하였다. 갯벌의 저서동물 분포는 노출시간 정도에 따라 구분되는 특성을 보였다. 즉, 상부에서 하부로, 노출시간이 감소하면서 Scopimera globosa, M. mai, E. spinigerus 군집이 뚜렷한 대상분포를 하였다. 양식장이 위치한 남서해역을 제외한 신두리 해안은 자연성을 가진 건강한 서식지였으며, 특히 천연기념물인 신두리 사구와 연결되는 갯벌과 주변 조하대 해역에는 인위적인 구조물이 거의 없으며 외부로부터의 오염원도 없어 향후 보전을 위한 가치가 충분히 있는 서식지로 판단되었다.

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Juniperus chinensis의 7변종(變種)의 세포학적(細胞學的) 특성(特性) (Cytological Studies of Seven Varieties of Juniperus chinensis)

  • 김정석;김영두;정우규
    • 한국산림과학회지
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    • 제71권1호
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    • pp.22-26
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    • 1985
  • Juniperus chinensis 7변종(變種)의 세포학적(細胞學的) 특성(特性)을 조사한 결과 J. chinensis var. procumbens, J. chinensis var. kaizuka와 J. chinensis var. aureo-variegate는 2n=44인 tetraploid이고, 여타 4개변종(個變種)은 2n=22인 diploid이다. 이들 tetraploid 변종(變種)들은 기공(氣孔)이 2n에 비하여 18.4% 크고, meiosis시(時)의 염색체(染色體) 행동(行動)이 불규칙(不規則)한 핵판(核板)이 관찰되었다.

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여수 연안해역에서 침편모조류 Chattonella속 출현환경 및 영양염에 대한 성장특성 (The Effect of Environmental Factors on the Advent of Chattonella (Raphidophyceae) in Yeosu Coastal Waters, Korea, and the Effect of Nutrients on the Growth of Chattonella)

  • 노일현;오석진;신현호;강인석;윤양호
    • 한국수산과학회지
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    • 제43권4호
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    • pp.362-372
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    • 2010
  • In order to understand what leads to the appearance of harmful Chattonella algae in the Yeosu coastal waters of Korea, we measured environmental parameters every week at one station from May to November, 2006, and April to October, 2007. Four species of Chattonella appeared during the monitoring period: C. antiqua, C. globosa, C. marina and C. ovata. The range of water temperature and salinity were $15.0-27.9^{\circ}C$ and 17.6~33.0 psu, respectively, when Chattonella appeared, and their maximum cell density (4,840 cells/L) was at $27.1^{\circ}C$ and 33.0 psu. During the monitoring periods, the range of dissolved inorganic nitrogen (DIN), phosphate (DIP) and chlorophyll $\alpha$ (Chl-$\alpha$) concentrations in surface waters were $1.20-52.23\;{\mu}M$ ($8.59{\pm}8.97\;{\mu}M$), $0.03-1.56\;{\mu}M$ ($0.47{\pm}0.31\;{\mu}M$) and $0.45-31.12\;{\mu}g/L$ ($3.58{\pm}4.77\;{\mu}g/L$), respectively. Chattonella occurred at low cell density when the Chl-$\alpha$ concentration increased because of supplied nutrients, whereas their cell density increased during the periods of rapid decrease in Chl-$\alpha$. The results of growth experiments based on batch culture showed that the half saturation constant ($K_s$) of C. antiqua on ammonium (${NH_4}^-$), nitrate (${NO_3}^-$) and phosphate (${PO_4}^{2-}$) were $3.89{\mu}M$, $5.01\;{\mu}M$ and $0.63\;{\mu}M$, respectively. These Ks values are higher than those reported for diatoms and other flagellates at the DIP concentration (average $0.47{\mu}M$) of Yeosu coastal waters. Although the maximum specific growth rate (${\mu}_{max}$) of C. antiqua was lower than diatoms, it was higher than those of other flagellates. Therefore, our results indicate that the DIP level in the study area was too low to support Chattonella blooms, although Chattonella species have physiological characteristics that enable them to grow more rapidly than other flagellates when nutrient levels are higher than their $K_s$.