• The Plant Pathology Journal
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• v.16 no.1
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• pp.9-18
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• 2000

Using differential display techniques, a new acidic pathogenesis-related (PR) protein-1 cDNA (GMPRla) gene was isolated from a cDNA library of soybean (Glycinemax L.Merr, cultivar Jangyup) hypocotyls infected by Phytophthora sojae f. sp. glycines. The 741 bp of fulllength GMPRla clone contains an open reading frame of 525 nucleotides encoding 174 amino acid residues (pI 4.23) with a putative signal peptide of 27 amino acids in the N-terminus. Predicted molecular weight of the protein is 18,767 Da. The deduced amino acid sequence of GMPRla has a high level of identity with PR-1 proteins from Brassica napus, Nicotiana tabacum, and Sambucus nigra. The GMPRla mRNA was more strongly expressed in the incompatible than the compatible interaction. The transcript accumulation was induced in the soybbean hypocotyls by treatment with ethephon or DL-$\beta$-amino-n-butyric acid, but not by wounding. In situ hybridization data showed that GMPRIa mRNAs were usually localized in the vascular bundle of hypocotyl tissues, especially phloem tissue. Differences between compatible and incompatible interactions in the timing of GMPRla mRNA accumulation were remarkable, but the spatial distribution of GMPRla mRNA was similar in both interactions. However, more GMPRla mRNA was accumulated in soybean hypocotyls at 6 and 24 h after inoculation.

• Publications of The Korean Astronomical Society
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• v.11 no.1
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• pp.27-47
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• 1996

We have performed the high resolution computer simulation with 1D spherical hydrodynamic code in order to study the dynamical evolution of supernova ejecta interacting with a pre-existing fast wind structure. The fast wind structure has been calculated with $M_{in}=3{\times}10^{-6}M_{\odot}yr^{-1}$ and ${\upsilon}_{in}=1000km/sec$, which velocity is higher than the critical velocity relating to the initial radiative cooling. The fast wind becomes initially adiabatic. After a shell formation time of ${\sim}4000yrs$, the wind becomes radiative cooling at the shell zone, forming a thin dense radiative shell and an adiabatic wind bubble afterward. When supernova explodes in the wind center at 20,000yrs after the wind evolves, the supernova ejecta, which has a dense distribution of ${\rho}{\propto}r^{-n}$(here we have n = 9), interacts initially with, the understood wind zone, producing forward and reverse shocks. The reverse shock heats the supernova ejecta and its temperature increases. In this study, as the mass of the supernova ejecta is larger than that of the wind shell ($M_{ej}=5M_{\odot}$, $M_{sw}=2M_{\odot}$), we can conform two shell structures: an outer shell by the supernova ejecta and a secondarily shocked wind shell by it. The secondarily shocked wind shell should accelerates in this case to be R-T unstable, consequently producing the knots.

• Journal of the Korean Magnetics Society
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• v.16 no.6
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• pp.287-292
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• 2006

Al substituted $CoAl_{0.5}Fe_{1.5}O_{4}$ has been studied with x-ray and neutron diffraction, $M\"{o}ssbauer$ spectroscopy and magnetization measurements. $CoAl_{0.5}Fe_{1.5}O_{4}$ revealed a cubic spinel structure of ferrinmagnetic long range ordering at room temperature, with magnetic moments of $Fe^{3+}(A)(-2.29{\mu}_{B}),\;Fe^{3+}(B)(3.81\;{\mu}_{B}),\;Co^{2+}(B)(2.66{\mu}_{B})$, respectively. The temperature dependence of the magnetic hyperfine field in $^{57}Fe$ nuclei at the tetrahedral (A) and octahedral (B) sites was analyzed based on the $N\'{e}el$ theory of magnetism. In the sample of $CoAl_{0.5}Fe_{1.5}O_{4}$, the interaction A-B interaction and intrasublattice A-A superexchange interaction were antiferromagnetic with strengths of $J_{A-B}=-19.3{\pm}0.2k_{B}\;and\;J_{A-A}=-21.6{\pm}0.2k_{B}$, respectively, while the intrasublattice B-B superexchange interaction was found to be ferromagnetic with a strength of $J_{B-B}=3.8{\pm}0.2k_{B}$.

• Journal of the Korea Concrete Institute
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• v.29 no.4
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• pp.353-360
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• 2017

The objective of this study is theoretical and empirical evaluation of the flexural performance of concrete filled pretensioned spun high strength concrete pile with ring type composite shear connectors (CFP pile). The specimens are comprised of standard CFP pile, PHC pile+composite shear connector+filed concrete (CFP-N-N), standard CFP pile with $1^{st}$ reinforcements (H13-8ea), and standard CFP pile with $1^{st}$ and $2^{nd}$ reinforcements(H19-8ea). Flexural performance evaluation results showed that the ductility is improved with increased steel ratio, which leads to the increased maximum load by 46.4% (with $1^{st}$ reinforcement) and 103.9% (with $1^{st}$ and $2^{nd}$ reinforcements) compared to standard CFP ( CFP-N-N). Comparing with the predicted ultimate limit state values of the CFP pile design method and the experimental results, the design method presented in this study is reasonable since safety factor of 1.23 and 1.40 times for each reinforcement step are secured.

• Journal of Magnetics
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• v.22 no.1
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• pp.155-161
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• 2017

This paper presents a new nonmechanical rehabilitation system driven by magnetic force. Typically, finger rehabilitation mechanisms are complex mechanical systems. The proposed method allows wireless operation, a simple configuration, and easy installation on the hand for active actuation by magnetic force. The system consists of a driving coil, driving magnets (M1), and auxiliary magnets (M2 and M3), respectively, at the finger, palm, and the center of coil. The magnets and the driving coil produce three magnetic forces for an active motions of the finger. During active actuations, magnetic attractive forces between M1 and M2 or between M1 and M3 enhance the flexion/extension motions. The proposed system simply improves the extension motion of the finger using a magnetic system. In this system, the maximum force and angular variation of the extension motion were 0.438 N and $49^{\circ}$, respectively. We analyzed the magnetic interaction in the system and verified finger's active actuation.

• Journal of the Korean institute of surface engineering
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• v.56 no.1
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• pp.40-54
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• 2023

In this work, we synthesized a novel electrochemical sensing materials based on tetracarboxylic copper phthalocyanine (TcCuPtc) conjugated PANI (TcCuPtc@PANI). The synthesized materials were employed to modify the screen-printed carbon electrode (SPCE) for the selective sensing of 4-nitrophenol. The TcCuPtc was conjugated with conducting polymer of PANI through the electrostatic interaction and π-π electron conjugation, the polymer film of PANI to inhibit the leakage of TcCuPtc from the surface of the electrode. The prepared TcCuPtc@PANI were characterized and confirmed by scanning electron microscopy (SEM) with EDX, ATR-IR, UV-vis absorption spectroscopy, cyclic voltammetry, and differential pulse voltammetry techniques. The prepared TcCuPtc@PANI/SPCE showed an excellent electrocatalytic sensing of 4-NP in the linear concentrations from 3 to 500 nM with a LOD of 0.03 nM and a sensitivity of 8.8294 ㎂/nM cm^-2. However, the prepared TcCuPtc@PANI/SPCE showed selective sensing of 4-NP in the presence of other interfering species. The practical applicability of the TcCuPtc@PANI/SPCE was employed for the sensing of 4-NP in different water samples by standard addition method and showed satisfactory recovery results.

• Bulletin of the Korean Chemical Society
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• v.31 no.11
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• pp.3241-3246
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• 2010

NHERF ($Na^+/H^+$ exchanger regulatory factor) and E3KARP (NHE3 kinase A regulatory protein) play important roles in membrane targeting, trafficking and sorting of ion channels, transmembrane receptors and signaling proteins in many tissues. Each of these proteins contains two PDZ (PSD-95/Dlg-1/ZO-1) domains, which mediate the assembly of transmembrane and cytosolic proteins into functional signal transduction complexes. The interaction between NHERF and E3KARP was investigated by surface plasmon resonance spectroscopy (BIAcore), fluorescence measurement, His-tagged pull-down experiment, and size-exclusion column (SEC) chromatography. BIAcore experiments revealed that NHERF bound to E3KARP with an apparent $K_D$ of 7 nM. Fluorescence emission spectra of the NHERF-E3KARP complex suggested that the tight interaction between these proteins was accompanied by significant conformational changes in one or both. The CD spectra of NHERF and E3KARP show that the conformational changes of these proteins were dependent on pH and temperature. These results implicate that the NHERF-E3KARP complex allows intracellular signaling complexes to form through PDZ-PDZ interactions.

• Structural Engineering and Mechanics
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• v.20 no.4
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• pp.421-434
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• 2005

In this paper, the finite element method is applied to investigate the effect of the lateral boundary in homogenous soil on the seismic response of a superstructure. Some influencing factors are presented and discussed, and several parameters are identified to be important for conducting soil-structure interaction experiments on shaking tables. Numerical results show that the cross-section width L, thickness H, wave propagation velocity and lateral boundaries of soil layer have certain influences on the computational accuracy. The dimensionless parameter L/H is the most significant one among the influencing factors. In other words, a greater depth of soil layer near the foundation should be considered in shaking table tests as the thickness of the soil layer increases, which can be regarded as a linear relationship approximately. It is also found that the wave propagation velocity in soil layer affects the numerical accuracy and it is suggested to consider a greater depth of the soil layer as the wave propagation velocity increases. A numerical study on a soil-structure experimental model with a rubber ring surrounding the soil on a shaking table is also conducted. It is found the rubber ring has great effect on the soil-structure interaction experiments on shaking table. The experimental precision can be improved by reasonably choosing the elastic parameter and width of the rubber ring.

• The Korean Journal of Physiology and Pharmacology
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• v.21 no.4
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• pp.385-395
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• 2017

Vasoconstrictive properties of sympathomimetic drugs are the basis of their widespread use as decongestants and possible source of adverse responses. Insufficiently substantiated practice of combining decongestants in some marketed preparations, such are those containing phenylephrine and lerimazoline, may affect the overall contractile activity, and thus their therapeutic utility. This study aimed to examine the interaction between lerimazoline and phenylephrine in isolated rat aortic rings, and also to assess the substrate of the obtained lerimazoline-induced attenuation of phenylephrine contraction. Namely, while lower concentrations of lerimazoline ($10^{-6}M$ and especially $10^{-7}M$) expectedly tended to potentiate the phenylephrine-induced contractions, lerimazoline in higher concentrations ($10^{-4}M$ and above) unexpectedly and profoundly depleted the phenylephrine concentration-response curve. Suppression of NO with NO synthase (NOS) inhibitor $N^w$-nitro-L-arginine methyl ester (L-NAME; $10^{-4}M$) or NO scavanger $OHB_{12}$ ($10^{-3}M$), as well as non-specific inhibition of $K^+$-channels with tetraethylammonium (TEA; $10^{-3}M$), have reversed lerimazoline-induced relaxation of phenylephrine contractions, while cyclooxygenase inhibitor indomethacin ($10^{-5}M$) did not affect the interaction between two vasoconstrictors. At the receptor level, non-selective 5-HT receptor antagonist methiothepin reversed the attenuating effect of lerimazoline on phenylephrine contraction when applied at $3{\times}10^{-7}$ and $10^{-6}M$, but not at the highest concentration ($10^{-4}M$). Neither the 5-$HT_{1D}$-receptor selective antagonist BRL 15572 ($10^{-6}M$) nor 5-$HT_7$ receptor selective antagonist SB 269970 ($10^{-6}M$) affected the lerimazoline-induced attenuation of phenylephrine activity. The mechanism of lerimazoline-induced suppression of phenylephrine contractions may involve potentiation of activity of NO and $K^+$-channels and activation of some methiothepin-sensitive receptors, possibly of the 5-$HT_{2B}$ subtype.

• Journal of the Korean Chemical Society
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• v.42 no.2
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• pp.190-196
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• 1998

A new sampling and size exclusion chromatography (SEC) method for the analysis of underivatized cellulose are established. In this method, cellulose materials are first dissolved in N-methylmorpholine N-oxide (NMMO) and diluted by adding dimethyl sulfoxide (DMSO) to make the sample solutions of about 0.1% in 50/50 NMMO/DMSO (w/w). Sample solutions are analyzed using a glucose-treated divinylbenzene (DVB) SEC column and DMSO containing 0.05M LiBr and 2.5 blank as the eluant. The flow rate was constant at 1 mL/min and the whole SEC system including the column was heated at $80^{\circ}C$ to reduce the viscosity of DMSO. Addition of 0.05 M LiBr eliminated SEC baseline drifting, and addition of 2.5 blank seems to reduce the interaction between the sample and the column packing. SEC molecular weights were determined using a calibration curve constructed from a series of narrow pullulan standards, and they were used to measure the degree of degradation during two different pulp-to-sponge processings.