• Bulletin of the Korean Chemical Society
• /
• v.28 no.6
• /
• pp.1005-1009
• /
• 2007

Leukotriene B4 (LTB4) is a potent chemoattractant for leukocytes and considered to be an inflammatory mediator. Human BLT2 (hBLT2) is a low-affinity G-protein coupled receptor for LTB4 and mediates pertussis toxin-sensitive chemotactic cell movement. Here, we dissected the interaction between hBLT2 and G-protein alpha subunits using GST fusion proteins containing intracellular regions of hBLT2 and various Gα protein including Gα i1, Gα i2, Gα i3, Gα s1, Gα o1, and Gα z. Among the tested Gα subunits, Gα i3 showed the highest binding to the third intracellular loop region of hBLT2 with a dissociation constant (KD) of 5.0 × 10?6 M. These results suggest that Gα i3 has the highest affinity to hBLT2, and the third intracellular loop region of hBLT2 is the major component for the interaction with Gα i3.

• Proceedings of the Korean Magnestics Society Conference
• /
• 1994.10a
• /
• pp.30-31
• /
• 1994

• Bulletin of the Korean Chemical Society
• /
• v.26 no.12
• /
• pp.2033-2037
• /
• 2005

In vitro selection was used to isolate $Mg^{2+}$-dependent self-cleaving ribozymes with cis-cleavage activity from a pre-tRNA library having 40-mer random sequences attached to 5'-end of E. coli $tRNA^{Phe}$. After 8 rounds of SELEX (Systematic Evolution of Ligands by Exponential Enrichment), RNA molecules which can self-cleave at the high concentration of $Mg^{2+}$ were isolated. The selected ribozymes can carry out the self-cleavage reaction in the presence of 100 mM $Mg^{2+}$ but not in 10 mM $Mg^{2+}$. The cleavage sites of the ribozymes are located at +3 and +4 of $tRNA^{Phe}$, compared with +1 position of 5'-end cleavage site of pre-tRNA by RNase P. New RNA constructs deprived of its D stem-loop, anticodon stem-loop, variable loop and T stem-loop, respectively showed the cleavage specificity identical to a ribozyme having the intact tRNA structure. Also, the new ribozyme fused with both a ribozyme and $tRNA^{Leu}$ showed the cleavage activities at the various sites within its sequences, different from two sites of position +3 and +4 observed in the ribozyme with $tRNA^{Phe}$. Our results suggest that the selected ribozyme is not structural-specific for tRNA.

• Nuclear Medicine and Molecular Imaging
• /
• v.43 no.4
• /
• pp.337-343
• /
• 2009

Purpose: ((R)-1-(2-chlorophenyl)-N-1-[$^{11}$C]methyl-N(1-propyl)-3-isoquinoline carboxamide ((R)-PK11195) is a specific ligand for the peripheral type benzodiazepine receptor and a marker of activated microglia, used to measure inflammation in neurologic disorders. We report here that a direct and simple radiosynthesis of [$^{11}$C](R)-PK11195 in mild condition using NaH suspension in DMF and one-step loop method. Materials and Methods: (R)-N-Desmethyl-PK11195 (1 mg) in DMSO (0.1 mL) and NaH suspension in DMF (0.1 mL) were injected into a semi-prep HPLC loop. [$^{11}$C]methyl iodide was passed through HPLC loop at room temperature. Purification was performed using semi-preparative HPLC. Aliquots eluted at 11.3 min were collected and analyzed by analytical HPLC and mass spectrometer. Results: The labeling efficiency of [$^{11}$C](R)-PK11195 was 71.8$\pm$8.5%. The specific activity was 11.8:$\pm$6.4 GBq/$\mu$mol and radiochemical purity was higher than 99.2%. The mass spectrum of the product eluted at 11.3 min showed m/z peaks at 353.1 (M+1), indicating the mass and structure of (R)-PK11195. Conclusion: By the one-step loop method with the [$^{11}$C]CH3l automated synthesis module, [$^{11}C$](R)-PK11195 could be easily prepared in high radiochemical yield using NaH suspension in DMF.

• Transactions of the Korean Society of Automotive Engineers
• /
• v.9 no.6
• /
• pp.186-194
• /
• 2001

To meet the challenge of testing increasingly complex automotive control systems, the real-time hardware-in-the-loop(HIL) simulation technology has been developed. In this paper, a strategy for evaluation of semiactive suspension systems using real-time HIL simulation is presented. A multibody vehicle model is adopted to simulate vehicle dynamic motions accurately. Accuracy of the vehicle simulation results is compared to that of the real vehicle field test and proven to be very accurate. The controller and stepping motor to adjust semi-active damper stage are equipped as external hardwares and connected to the real-time computer which has vehicle dynamic model. Open and closed loop test methods are used to evaluate a controlled suspension system and the system's operations are verified it is found that the proposed evaluation methods can be used well for the verification of semi-active suspension systems.

• Journal of ILASS-Korea
• /
• v.8 no.1
• /
• pp.23-28
• /
• 2003

When a cold HPSI (High Pressure Safety Injection) fluid associated with a design basis accident, such as LOCA (Loss of Coolant Accident), enters the cold legs of a stagnated primary coolant loop, thermal stratification phenomena may arise due to incomplete mixing. If the stratified flow enters a reactor pressure vessel downcomer, severe thermal stresses are created in a radiation embrittled vessel wall by local overcooling. Previous thermal-mixing analyses have assumed that the thermal stratification phenomena generated in stagnated loop of a partially stagnated collant loop are neutralized in the vessel downcomer by strong flow from unstagnated loop. On the basis of these reasons, this paper presents the thermal-mixing analysis results in order to identify the fact that the cold plume generated in the vessel downcomer due to the thermal stratification phenomena of the stagnated loop is affected by the strong flow of the unstagnated loop.

• Proceedings of the KSME Conference
• /
• 2007.05b
• /
• pp.2130-2135
• /
• 2007

Operating characteristics of a loop heat pipe (LHP) having a bypass line was investigated experimentally. The LHP had a sintered metal wick as a capillary structure and methanol as a working fluid. The sintered metal wick was made of stainless steel of which the average pore size was 5 ${\mu}m$and porosity of 47%. A bypass line of a small diameter was attached between the vapor escape passage and the liquid reservoir. The dimension of the flat evaporator was $50(L){\times}40(W){\times}30(H)$ mm and that of the condenser was $50(L){\times}40(W){\times}11(H)$ mm. Wall and pipe material of the LHP was stainless steel and heating area was 35(W) mm${\times}$35(L) mm. The inner diameters of vapor and liquid transport lines were 4.0 mm and 2.0 mm, and the lengths of the two lines were both 0.5 m. The LHP was tested for three different tilt angles of horizontal, favorite tilt, and adverse tilt. The thermal load range was up to 290 W at the condenser above evaporation position. Furthermore, the effect of a bypass line on the start-up transient as well as steady-state operation was presented and discussed.

• Journal of Magnetics
• /
• v.1 no.2
• /
• pp.69-74
• /
• 1996

Justeresos loops of melt-spun Nd13Fe77B10 cooled down at remanent state were measured at 4.2 K and 250 K. The hysteresis loops were analysed on the basis of the Stoner-Wohlfarth (S-W) model, the inter-grain exchange coupled single domain (SD) model and micromagnetism. The coercivity higher than that predicted from the S-W model and the striking shift of the thin minor loop along the H-axix observed at the fields of Hmax=4MA/m at 4.2 K indicated new evidences for the inter-grain exchange interaction . The S-W model failed in explaining the high iHc and the shift of the thin minor loop. The exchange coupled SD model was found to explain the experimental results qualitatively without difficulties associated with the S-W model. The micromagnetic calculations using a finite element technique simulated the experimental results fairly well quntitatively.

• Proceedings of the KSME Conference
• /
• 2004.04a
• /
• pp.905-910
• /
• 2004

Vibration characteristics of the FIV test loop for the Flow-Induced Vibration(FIV) study of a PWR partial(5x5) fuel assembly are investigated by the Finite Element(FE) analysis and the modal test. For the FE analysis, 3-D beam element is used for the pipes and the test section and mass element used for the valves and flanges. The 'U' restrainer stiffness determined by numerical simulation is used for the FE model. The result of the FE analysis is compared with that of the modal test. The higher mode similarity between the test and analysis is observed in a few low modes. After that, the mode similarity reduce as the mode goes high. It is concluded that the first to the third vibration modes are observed at the lower parts of the 6 inches restoring line, followed by a local mode at the test section, and the natural frequencies of the modes are 22.4 Hz, 26.0 Hz, 27.5 Hz and 31.4 Hz.

• Progress in Superconductivity
• /
• v.6 no.1
• /
• pp.84-88
• /
• 2004

The polycrystalline superconductor $SmBa_2$$Cu_3$$O_{x}$ is fabricated, and intergranular magnetic properties are investigated using the critical state model, from which some useful parameters such as the critical current density and the intergranular volume fraction are obtained. The curve fitting for M-H hysteresis loop shows that the intergranular critical current density of $SmBa_2$$Cu_3$$O_{x}$ / decreases in the form of ($1-T/T_{c}$ )$^{1.5}$ . The intergranular volume fraction is influenced by granular morphology. From SEM image, the grains of $SmBa_2$$Cu_3$$O_{x}$ are found to be randomly shaped. This mean:; that the intergranular volume fraction of $SmBa_2$$Cu_3$$O_{x}$ / should be smaller than those of superconductors, of which grains are plate-shaped such as Tl-based superconductor.