• Korean Journal of Food Science and Technology
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• v.33 no.1
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• pp.33-39
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• 2001

To develop a method for separation process using Sep-pak $C_18$, simultaneous and systematic analysis of 8 permitted and 11 non-permitted synthetic food colors in Korea, optimization of analysis conditions for reverse phase ion-pair high performance liquid chromatography was carried out. For the best result of Sep-pak $C_18$ separation the pH of color standard mixture solution was $5{\sim}6$ and 0.1% HCl-methanol solution were set as eluent. The colors eluated from Sep-pak $C_18$ cartridge were determined and confirmed by high performance liquid chromatography with a photodiode array detector at 420 nm for yellow colors type, at 520 nm for red colors type, at 600 nm for blue and green colors type and at 254 nm for mixed colors. Conditions for HPLC analysis were as follows: column, Symmetry $C_18$ (5 m, 3.9 mm $i.d.{\times}150\;mm$); mobile phase, 0.025 M ammonium acetate (containing 0.01 M tetrabutylammonium bromide) : acetonitrile : methanol (65 : 25 : 10) and 0.025 M ammonium acetate(containing 0.01 M tetrabutylammonium bromide) : acetonitrile : methanol (40 : 50 : 10); flow rate, 1 mL/min. It takes 35 minutes for simultaneaus analysis and 18 minutes for systematic analysis. The detection limits range of each colors were $0.01{\sim}0.05\;{\mu}g/g$.

• Magazine of the Korean Society of Agricultural Engineers
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• v.15 no.3
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• pp.3096-3106
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• 1973

As a link in the chain of antidrought measure, our attempt is made to obtain basic informations on the construction of an infiltration gallary which can be supplied with irrigation water by catching of underground water in small river beds, which is economical, permanent and efficient. The experiment was made, concerning the structure of catchment conduits, by constructing a model sand tank $1.5m{\times}5m{\times}1.5m$ in dimension made of reinforced concrete. Various kinds of measuring equipment were attached to the model tank which contains a set of catchment conduits, each of them was made 30cm in diameter and 60cm in length with the ratio of sectional area to total area of influx holes 10:1, 20:1, 30:1. The average size of influx holes was fixed from 0mm to 10mm, 20mm and 30mm in diameter respectively. Obtained results are as follow; (a) In view of the water catchment capacity, manufacturing cost and the antipressure strength of the catchment conduits, it is the best method to decide the total number of influx holes 20 per sq. meter of each tile surface, and the size of influx holes 20mm in diameter, when the conduits have diameter less than 1m. (b) The greatest factor of safety against external load is to arrange the influx holes in a zigzag manner on the tile surface. The most effective formula of arrangement is $S{\geqq}\sqrt{2gd}$ where: s : spacing of opening row. g : spacing of opening line. d : diameter of influs hole.

• Proceedings of the IEEK Conference
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• 2003.07b
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• pp.751-754
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• 2003

Metamorphic HEMTs (MHEMTs) have emerged as excellent challenges for the design and fabrication of high-speed HEMTs for millimeter-wave applications. Some of improvements result from improved mobility and larger conduction band discontinuity in the channel, leading to more efficient modulation doping, better confinement, and better device performance compared with pseudomorphic HEMTs. We have studied the calibration on the DC and RF characteristics of the MHEMT device using I $n_{0.53}$G $a_{0.47}$As/I $n_{0.52}$A1$_{0.48}$As modulation-doped heterostructure on the GaAs wafer. For the optimized device performance simulation, we calibrated the device performance of 0.1-${\mu}{\textrm}{m}$ $\Gamma$-gate MHEMT fabricated in our research center using the 2D ISE-DESSIS device simulator. With this calibrated parameter set, we have obtained very good reproducibility. The device simulation on the DC and RF characteristics exhibits good reproducibility for our 0.1-${\mu}{\textrm}{m}$ -gate MHEMT device compared with the measurements. We expect that our calibration result can help design over-100-GHz MHEMT devices for better device performance.ormance.

• Journal of Applied Biological Chemistry
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• v.59 no.4
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• pp.373-377
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• 2016

An extracellular metalloprotease, Btmp, was partially purified from the culture supernatant of Bacillus thuringiensis 29-126, isolated from animal feces collected in a zoological garden in Japan, by ultrafiltration, ammonium sulfate precipitation, and a set of chromatography on Sephadex G-75 and High-Q. The molecular mass of the protease was estimated to be 60 kDa by SDS-PAGE. The enzyme showed optimum activity at $50^{\circ}C$ and pH 6.0, and had a half-life of 14 min at $50^{\circ}C$. The enzyme activity was not influenced by $Na^+$, $K^+$, $As^+$, $Mg^{+2}$, $Ca^{2+}$, $Ba^{2+}$, and phenylmethylsulfonyl fluoride, but it was moderately inhibited by $Zn^{+2}$ at a concentration of 1.0 mM, while the activity was significantly inhibited to less than 50 % by $Cu^{2+}$, $Co^{2+}$, $Cd^{2+}$, and ethylenediaminetetraacetic acid. Interestingly, the enzyme was activated to 178 % by 1.0 mM of $Mn^{2+}$. From these results, it may be suggested that the protease is a novel extracellular manganeseactivated metalloprotease.

• Economic and Environmental Geology
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• v.3 no.2
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• pp.123-133
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• 1970

위에서 상논(詳論)한것을 요약(要約)하면, 1) 본(本) 지성(地城)을 구성(構成)하는 지질(地質)은 신나통(新羅統)의 퇴적암류(堆積岩類), 이를 관입(貫入)하는 불국사순(佛國寺純)의 화강록암(花崗綠岩) 및 암맥(岩脈)으로 되어 있다. 2) 광구일대(鑛區一帶)의 퇴적암류(堆積岩類)는 원내(原來)의 퇴적상(堆積相)과 화강섬록암(花崗閃綠岩)의 관입등(貫入等)으로 규화(珪化)되 어 규암(珪岩) 또는 규화(珪化)된 혼펠스로 변(變)하여 있다. 3) 지질구조(地質構造)는 단순(單純)한 배사(背斜)를 나타내 며 퇴적암(堆積岩) 및 화강섬록암중(花崗閃綠岩中)에 수(數) sets의 절이(節理)가 발달(發達)하여 이들에 따라 광화작용(鑛化作用)이 이루어졌다. 4) 광상(鑛床)은 퇴적암(堆積岩) 및 화강섬록암중(花崗閃綠岩中)의 열하에 연(沿)하여 배태(胚胎)하며 연(軟)망간광(鑛) 광맥(鑛脈)과 금은광맥(金銀鑛脈)의 2종(種)이 발달(發達)된다. 5) 금은광맥(金銀鑛脈)은 맥폭(脈幅)이 좁고 주향방향(走向方向)의 연장(延長)이 단속(斷續)되 며 분석결과(分析結果) 금(金)은 없고 은(銀)은 23.3g/t 내지(乃至) 913.3g/t 이여서 가행가치(稼行價直)가 없다. 6) 망강광맥(鑛脈)은 수조(數條)있으나 주목(注目)만한 것은 제(第)4갱맥(坑脈)과 제(第)5갱맥(坑脈)으로서, 전자(前者)는 연장(延長) 120m, 평균맥폭(平均脈幅) 0.5m 추정(推定)되며, 평균품위(平均品位)는 Mn 약(約) 17.5%이다. 후자(後者)도 연장(延長) 120m, 평균맥폭(平均脈幅) 0.6m 추정(推定)되며, 평균품위(平均品位)는 15.7%이다. 7) 광맥(鑛脈)은 후화강섬록암시기(後花崗閃綠岩時期)의 열하의 방위(方位)에 지배(支配)받으며, 주향(走向) 및 경사방향(傾斜方向)으로 전변(轉變)한다. 그러나 규암질모암(珪岩質母岩)의 성질(性質)로 보아 광상(鑛床)이 팽대(膨大)하여질 가능성(可能性)은 없다. 8) 망간 및 금은광맥(金銀鑛脈)은 열수광상(熱水鑛床)에 속(屬)한다.

• Molecules and Cells
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• v.40 no.11
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• pp.828-836
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• 2017

Eukaryotic cells consist of a complex network of thousands of proteins present in different organelles where organelle-specific cellular processes occur. Identification of the subcellular localization of a protein is important for understanding its potential biochemical functions. In the post-genomic era, localization of unknown proteins is achieved using multiple tools including a fluorescent-tagged protein approach. Several fluorescent-tagged protein organelle markers have been introduced into dicot plants, but its use is still limited in monocot plants. Here, we generated a set of multicolored organelle markers (fluorescent-tagged proteins) based on well-established targeting sequences. We used a series of pGWBs binary vectors to ameliorate localization and co-localization experiments using monocot plants. We constructed different fluorescent-tagged markers to visualize rice cell organelles, i.e., nucleus, plastids, mitochondria, peroxisomes, golgi body, endoplasmic reticulum, plasma membrane, and tonoplast, with four different fluorescent proteins (FPs) (G3GFP, mRFP, YFP, and CFP). Visualization of FP-tagged markers in their respective compartments has been reported for dicot and monocot plants. The comparative localization of the nucleus marker with a nucleus localizing sequence, and the similar, characteristic morphology of mCherry-tagged Arabidopsis organelle markers and our generated organelle markers in onion cells, provide further evidence for the correct subcellular localization of the Oryza sativa (rice) organelle marker. The set of eight different rice organelle markers with four different FPs provides a valuable resource for determining the subcellular localization of newly identified proteins, conducting co-localization assays, and generating stable transgenic localization in monocot plants.

• Journal of The Korean Astronomical Society
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• v.37 no.4
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• pp.143-149
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• 2004

We present photometric results for four new variable stars discovered in the vicinity of the ZZ Ceti-type pulsating white dwarf BR Cam. Observations were performed on 5 nights in November 2003 using the 1.8m telescope at Bohyunsan Optical Astronomy Observatory with no filter, on 3 nights in December 2003 using the 0.61m telescope at Sobaeksan Optical Astronomy Observatory with V, I filters, and on 3 nights in October 2004 using the 1.0m telescope at Mt. Lemmon Optical Astronomy Observatory with V, I filters. We estimated their periods from the phase-match technique for one eclipsing binary and the multiple frequency analysis for three pulsating stars. By considering the light curve shape, period and amplitude difference between two passbands, we classified the objects by their variability types as follows: V1 (USNO-A2.0 1425-05691757) is a W UMa-type eclipsing binary with an orbital period of $0^d.4641$; V2 (USNO-A2.0 1425-05703335) is a multi-periodic $\delta$ Set-type pulsating star with a dominant period of $0^d.0649$; V3 (USNO-A2.0 1425-05699659) is also a $\delta$ Set-type pulsating star with a period of $0^d.1408$; and V 4 (USNO-A2.0 1425-05707705) is a RR Lyr-type pulsating star with a period of $0^d.2643$.

• Journal of Aquaculture
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• v.13 no.3
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• pp.223-230
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• 2000

A rearing experiment of the olive flounder was performed in a set of water-reuse system to test the reusability of the water in culture system with (i) a foam fractionator to separate particles from water and (ii) a culture tank contain mineral particles to filter the metabolic wastes by adsorption and/or decomposition. Two kinds of commercially processed loess particles and a dolomite particle (all 50 ${\mu}$diameter) were tested. The mineral particles were suspended in the culture tank and the water was pumped into the foam fractionator, where the particles were separated and drained out with foam from the system. In a circular culture tank of 4.8 m in diameter with 10 d water, the juvenile olive flounders (23.1 g/fish, 5,555 fish, 128 kg total body weight) were stocked. 90 % of the rearing water was reused and turnover rate of the water in the tank was two times per hour. Water temperature was maintained 17${\pm}$1$^{\circ}C$. At the end of 75 day-experimental rearing, 5,532 flounders, weighing 468 kg, were harvested. An individual flounder grew to 84.6 g of body weight. The final stocking density was 26.0 kg/$m^2$. No diseases were observed during the experiment.

• Resources Recycling
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• v.15 no.4 s.72
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• pp.36-43
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• 2006

There was some difficulty dewatering properties due to small porosity diameter of cake, when pigment sludge contained fine particle was formed by cake under the dewatering. It was difficult to dewater the sludge with fine particles with the conventional mechanical dewatering method. This study was to improve the dewatering rate as discharging the water from porosity of cake easily, supplying the low heat to the cake layer. Thermal dewatering equipment of piston type to keep up constant temperature on the cake was set up and relative experiment was conducted for sludge of 200 g with fine pigment particle. As test results. filtration of 176.8 g, cake weight of 19.4 g, cake thickness of 4.2 mm was measured, and it was analyzed that the water content of cake was 47 wt% and dewatering velocity, which moaned the residual d교 sloid amount per dewatering area, was $2.1DS\;m^{2}{\cdot}cycle$. This results showed that filtration increased, cake weight and thickness decreased and dewatering velocity increased against mechanical dewatering method. And water content of cake decreased about 30%, so the result which dewatering rate improved was drew generally. The reason is that the inner vapor pressure working at the cake porosity increased as applying the low heat to the cake layer, which lead to discharge the water from porosity easily. Therefore, this study was estimated by the useful technology for sludge reduction.

• Journal of Life Science
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• v.29 no.1
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• pp.105-111
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• 2019

In recent years, progress has been made in the search for the development of new anti-cancer agents by employing specific inhibitors of histone deacetylase (HDAC)-6 to block signal transduction pathways in cancer cells. This study examined the effects of tubastatin A (TubA), an HDAC-6 inhibitor, on the growth and development of immature oocytes in murine ovaries using RNA sequencing analysis. The results from a gene set enrichment analysis (GSEA) indicated that the expression of most of the gene sets involved in the cell cycle and control and progression of meiosis decreased in the TubA-treated group as compared with that in germinal vesicle (GV) stage oocytes. In addition, an ingenuity pathway analysis (IPA) suggested that TubA not only caused increased expression of p53 and pRB and decreased expression of CDK4/6 and cyclin D but also caused elevated expression of genes involved in the control of the DNA check point in G2/M stage oocytes. These results suggest that TubA may induce cell cycle arrest and apoptosis through the induction of changes in the expression of genes involved in signal transduction pathways associated with DNA damage and the cell cycle of immature oocytes in the ovary.