• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2017.05a
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• pp.767-769
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• 2017

Four-shell open-typed shield case was analyzed using 3D FEM. Analysis Dimension was $2m{\times}2m{\times}2m$. Length of shield case was 0.6m and its diameter was 0.1m, 0.08., 0.06m and 0.04m. Thickness and permeablility of shield case was all 1mm and 50,000. The excited magneic fields were earth magneic fields, which were 24A/m in the holizontal direction and 36A/m in the vertical direction, respectively. During FEM analysis, shield case was located at the direction of holizontal magnetic field and was rotated $90^{\circ}$. Magnetic field was $4.45{\times}10^{-2}A/m$ at the direction of holizontal magnetic field and $6.66{\times}10^{-4}A/m$ at the $90^{\circ}$ rotated direction.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.29 no.1
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• pp.64-70
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• 1996

Kinetic properties of typsins purified from dark-fleshed fish (anchovy, mackerel, yellowfin tuna, and albacore) were examined and analyzed on $benzoyl-_{D,L}-arginine-p-nitroanilide\;(BAPNA)$. The values of Km' and $k_{cat}$ of the purified trypsins from the four dark-fleshed fish were found to be $49.3{\mu}M$ and $90.9\;min^{-1}$ for anchovy, $53.7{\mu}M$ and $61.2min-^{-1}$ for mackerel A, $96.5{\mu}M$ and $76.6min^{-1}$ for mackerel B, $62.8{\mu}M$ and $46.6min^{-1}$ for yellowfin tuna, and $98.3{\mu}M$ and $47.7min^{-1}$ for albacore, respectively. The values of $K_i$ on $tosyl-_L-lysine$ chloromethyl ketone (TLCK) were determined to be $20.90{\mu}M$ for anchovy trypsin, $2.86{\mu}M$ for mackerel trypsin A, $3.90{\mu}M$ for mackerel trypsin B, $0.96{\mu}M$ for yellowfin tuna trypsin, and $1.82{\mu}M$ for albacore trypsin. Thus yellowfin tuna trypsin was the most sensitive to TLCK among all trypsins. The activities and catalytic efficiency of the trypsins purified from the temperate zone fish, anchovy and mackerel, were higher than those of the trypsins purified from yellowfin tuna and albacore which migrate widely from the tropic zone to the temperate zone.

• Environmental Analysis Health and Toxicology
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• v.29
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• pp.2.1-2.7
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• 2014

Objectives Heat shock protein 90 (HSP90) is a highly conserved molecular chaperone important in the maturation of a broad spectrum of protein. In this study, an HSP90 gene was isolated from Asian paddle crab, Charybdis japonica, as a bio-indicator to monitor the marine ecosystem. Methods This work reports the responses of C. japonica HSP90 mRNA expression to cellular stress by endocrine disrupting chemicals (EDCs), such as bisphenol A (BPA) and 4-nonylphenol (NP) using real-time. reverse transcription polymerase chain reaction. Results The deduced amino acid sequence of HSP90 from C. japonica shared a high degree of homology with their homologues in other species. In a phylogenetic analysis, C. japonica HSP90 is evolutionally related with an ortholog of the other crustacean species. The expression of HSP90 gene was almost distributed in all the examined tissues of the C. japonica crab but expression levels varied among the different body parts of the crabs. We examined HSP90 mRNA expression pattern in C. japonica crabs exposed to EDCs for various exposure times. The expression of HSP90 transcripts was significantly increased in C. japonica crabs exposed to BPA and NP at different concentrations for 12, 24, 48 and 96 hours. The mRNA expression of HSP90 gene was significantly induced in a concentration- and time-dependent manner after BPA or NP exposures for 96 hours. Conclusions Taken together, expression analysis of Asian paddle crab HSP90 gene provided useful molecular information about crab responses in stress conditions and potential ways to monitor the EDCs stressors in marine environments.

• Korean Journal of Animal Reproduction
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• v.22 no.4
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• pp.385-393
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• 1998

This study was conducted to investigate the effect of cysteine (CySH) and glutathione (GSH) on in vitro maturation of porcine follicular oocytes. The results obtained were summarized as follows: 1. When the immature oocytes were cultured at 0, 0.04, 0.14, 0.6 and 1.2 mM of cysteine (CySH) for 36h, the germinal vesicle breakdown (GVBD) rates were 90.8, 89.9, 90.5, 92.0 and 91.3%, respectively, and the maturation rates of the oocytes with metaphase-II were 56.1, 50.7, 41.9, 49.0 and 61.5%, respectively. Especially, the maturation rates of 0.14 and 0.6 mM treated groups were significantly lower than those of control (non-treated) group (P＜0.05). After 44h of culture in the same treatments of CySH, the GVBD rates of porcine immature oocytes were 90.0, 91.8, 89.8, 90.5 and 89.6%, respectively, and the maturation rates were 80.2, 76.3, 69.4, 66.7 and 72.6%, respectively. Especially, the maturation rates of 0.14 and 0.6 mM treated groups were significantly lower than those of control group (P＜0.05). 2. When the immature oocytes were cultured at 0, 0.05, 0.1, 0.5 and 1.0 mM of glutathione (GSH) for 36h, the GVBD rates of porcine immature oocytes were 91.0, 90.9, 89.5, 92.0 and 91.1%, respectively, and the maturation rates were 59.0, 48.5, 47.8, 38.6 and 37.5%, respectively. All treated groups of GSH showed lower maturation rates than the control group (P＜0.05). After 44h of culture in the same treatments of GSH, the GVBD rates of porcine immature oocytes were 91.8, 94.1, 89.1, 91.3 and 91.1%, respectively, and the maturation rates were 84.6, 57.1, 69.6, 71.3 and 64.3% respectively. All treated groups of GSH showed lower maturation rates than the control group (P＜0.05).

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.36 no.3
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• pp.261-265
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• 2023

In this paper, we analyzed the transformation of the power following by the angle of incidence of the solar, the angle of photovoltaic module and artificial solar changed from 30° to 90° and synchronously changed the distance from 0.1 m to 0.5 m. Setting the distance between the artificial solar and the luminometer from 0.1 m to 0.5 m and set the angles to 90°, 60°, 45°, and 30°, the angle was 90° and when the distance was 0.1 m, the maximum Illuminance was 19,580 lux, the light could be obtained more. If the angle of incidence between the Artificial solar and the photovoltaic module was 90° and the variable resistance was 1,000 Ω at a distance of 0.4 m, the maximum power reached 0.82 W. Provided that the angle of incidence between the artificial solar and the photovoltaic module was 90° and the distance was 0.2 m since the variable resistance had the maximum power of 500 Ω, the maximum power was 0.78 W. At 1,000 Ω, the maximum power is 0.80 W so the maximum power at the variable resistance 1,000 Ω could obtain higher power than the variable resistance 500 Ω. The variable resistance was 1,000 Ω and the angle of incidence between the Artificial solar and the photovoltaic module was 90° at a distance of 0.4 m, and the maximum power reached 0.82 W. The angle was 60° at 0.3 m and 0.4 m the maximum power reached 0.10 W. The angle was 45° at 0.2 m maximum power reached 0.020 W, the angle was 30° at 0.4 m, and the maximum power reached 0.004 W. In four results about maximum power depending on the angle of incidence between the artificial solar and the photovoltaic module, the luminous efficiency and maximum power can be got the best at an angle of 90°.

• Biomedical Science Letters
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• v.30 no.2
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• pp.96-99
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• 2024

In vitro antimicrobial activities of hot water extracts of Chamaecyparis obtuse, for clinical metallo-β-lactamase-Producing Pseudomonas aeruginosa (MBLPA.) was compared to commonly used conventional antimicrobial agents. All MBLPA was susceptible to colistin or amikacin, but also to imipenem 88.6%, meropenem 100%, piperacillin 85.7%, ceftazidime 97.1%, gentamicin 97.1%, and ciprofloxacin 100% were non-susceptible. MIC range to imipenem, meropenem, cefotaxime, ceftazidime, gentamicin, and ciprofloxacin for MBLPA were each 1 - >128 ㎍/mL, 4 - >128 ㎍/mL, 4 - >128 ㎍/mL, 8 - >128 ㎍/mL, 4 - >128, and 2- >128 ㎍/mL. MIC range to aztreonam for MBLPA were 1 - 128 ㎍/mL. MIC₉₀ to imipenem, meropenem, cefotaxime, ceftazidime, gentamicin, and ciprofloxacin for MBLPA were each 32 ㎍/mL, >128 ㎍/mL, >128 ㎍/mL, >128 ㎍/mL, >128 ㎍/mL, and 128 ㎍/mL. MIC₉₀ to colistin and amikacin were each 1 ㎍/mL and 64 ㎍/mL. The hot water extracts of C. obtuse leaf had the lowest MIC range (0.25 - >0.5 μL/mL), MIC₅₀ (>0.5 μL/mL), and MIC₉₀ (>0.5 μL/mL) of the clinical MBLPA tested, and it was possible more potent than various conventional antimicrobial agents for MBLPA infection patients. Therefore, it suggested the possibility of using extract components of C. obtuse or their derivatives to treat MBLPA infection patients.

• Resources Recycling
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• v.31 no.5
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• pp.20-25
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• 2022

In this study, the applicability of microalgae was evaluated for eco-friendly decontamination of cesium-137 (Cs-137) and strontium-90 (Sr-90), which are radioactive nuclides contained in radioactive waste. The monolithic radioactive solution used in the experiment was manufactured at a concentration of 1.5 Bq/mL Cs-137 and 1.0 Bq/mL Sr-90 by diluting a standard radioactive solution and distilled water. This experiment used two types of microalgae, Chlorella Vulgaris was used for Sr-90 decontamination and Hematococcus pluvialis for Cs-137 decontamination. The experimental method is to put the microalgae cultured for 2 weeks into a bottle with a semi-permeable membrane, and then put the bottle in which the microalgae was put into the manufactured radioactive solution, so that the microalgae and the radioactive solution react through the semi-permeable membrane for 48 hours. For the radioactivity concentration analysis of each sample, a gamma-ray nuclide analyzer was used for Cs-137, a γ-ray isotope, and a Liquid Scintillation Count(LSC) was used f or Sr-90, a β-ray isotope. As a result of the experiment, it was confirmed that about 88.0 % of Cs-137 and about 89.7 % of Sr-90 could be decontaminated, and about 98.6 % of Sr-90 was finally able to be decontaminated by the two-stage decontamination method.

• Journal of agriculture & life science
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• v.46 no.4
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• pp.113-118
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• 2012

Effects of plant activators on germination of primed pepper seeds were investigated. The percent germination and T50 of primed seeds with 0.1mM ASM were 98% and 0.96 day at the 7 days after seeding, respectively. However, the germination of primed seeds with 0.5mM ASM were suppressed as 17% at the 7 days after seeding. The percent germination of primed seeds with 0.01mM INA were 90% at the 2 days after seeding. However, those of primed seeds by 0.1mM INA were increased as 90% at the 5 days after seeding. The percent germination of primed seeds was not change by treatment of the BABA and JA.

• Molecules and Cells
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• v.41 no.5
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• pp.436-443
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• 2018

The actin cytoskeleton plays a key role in the entry of mitosis as well as in cytokinesis. In a previous study, we showed that actin disruption delays mitotic entry at G2/M by sustained activation of extracellular signal-related kinase 1/2 (ERK1/2) in primary cells but not in transformed cancer cell lines. Here, we examined the mechanism of cell cycle delay at G2/M by actin dysfunction in IMR-90 normal human fibroblasts. We observed that de-polymerization of actin with cytochalasin D (CD) constitutively activated ribosomal S6 kinase (RSK) and induced inhibitory phosphorylation of Cdc2 (Tyr 15) in IMR-90 cells. In the presence of an actin defect in IMR-90 cells, activating phosphorylation of Wee1 kinase (Ser 642) and inhibitory phosphorylation of Cdc25C (Ser 216) was also maintained. However, when kinase-dead RSK (DN-RSK) was overexpressed, we observed sustained activation of ERK1/2, but no delay in the G2/M transition, demonstrating that RSK functions downstream of ERK in cell cycle delay by actin dysfunction. In DN-RSK overexpressing IMR-90 cells treated with CD, phosphorylation of Cdc25C (Ser 216) was blocked and phosphorylation of Cdc2 (Tyr 15) was decreased, but the phosphorylation of Wee1 (Ser 642) was maintained, demonstrating that RSK directly controls phosphorylation of Cdc25C (Ser 216), but not the activity of Wee1. These results strongly suggest that actin dysfunction in primary cells activates ERK1/2 to inhibit Cdc2, delaying the cell cycle at G2/M by activating downstream RSK, which phosphorylates and blocks Cdc25C, and by directly activating Wee1.

• Journal of Dental Rehabilitation and Applied Science
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• v.16 no.1
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• pp.13-25
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• 2000

Optimal Pressable Ceramic is one of the all-ceramic restorations with a shaded translucent pressed core and layering porcelains. The purpose of this study was to evaluate the marginal fidelity according to margin types and measurement sites, and to evaluate fracture strength according to margin types. Twenty seven OPC crowns made according to 3 types of cervical finishing lines were used in this study. Marginal gaps were measured before and after cementation. A Steromicroscope(SZ-ST(R), Olympus, Japan) was used to measure the space between the margin of OPC crown and the finishing line of metal model. Marginal gaps were measured at the labial, mesial, lingual and distal site, which were demonstrated in advance. Fracture strength testing was carried out using an Instron(Model M100EC, Mecmesin, England) at a cross head speed of 5 mm/min. All crowns were loaded until catastrophic failures occurred. The result were as follow: 1. In comparison according to variable margin before cementation, the marginal gap were increased in chamfer margin($47.50{\pm}18.39{\mu}m$), $120^{\circ}$shoulder margin ($55.21{\pm}14.4{\mu}m$) and $90^{\circ}$shoulder margin($71.18{\pm}13.30{\mu}m$) in ascending order, and there were significant differences between chamfer margin and $90^{\circ}$shoulder margin, $120^{\circ}$shoulder margin and between $120^{\circ}$shoulder margin and $90^{\circ}$shoulder margin respectively(p<0.05). 2. In comparison according to variable margin after cementation, the gap discrepancies were increased in chamfer margin($60.78{\pm}30.37{\mu}m$), $120^{\circ}$shoulder margin($66.67{\pm}11.18{\mu}m$) and $90^{\circ}$shoulder margin($85.78{\pm}17.23{\mu}m$) in ascending order, but there was significant difference only between chamfer margin and $90^{\circ}$shoulder margin(p<0.05). 3. Labio-lingual points showed a better marginal fidelity than that of proximal point(p<0.05). 4. Chamfer margin($48.76{\pm}8.45kgf$) showed higher fracture strength than $120^{\circ}$ shoulder margin($40.57{\pm}7.90kgf$) and $90^{\circ}$ shoulder margin(32.7.90kgf) (p<0.05), but there was significant difference only between chamfer margin and $90^{\circ}$ shoulder margin(p<0.05).