Effect of Cysteine and Glutathione on In Vitro Maturation of Porcine Follicular Oocytes

Cysteine 및 Glutathione 이 돼지난포란의 체외성숙에 미치는 영향

This study was conducted to investigate the effect of cysteine (CySH) and glutathione (GSH) on in vitro maturation of porcine follicular oocytes. The results obtained were summarized as follows: 1. When the immature oocytes were cultured at 0, 0.04, 0.14, 0.6 and 1.2 mM of cysteine (CySH) for 36h, the germinal vesicle breakdown (GVBD) rates were 90.8, 89.9, 90.5, 92.0 and 91.3%, respectively, and the maturation rates of the oocytes with metaphase-II were 56.1, 50.7, 41.9, 49.0 and 61.5%, respectively. Especially, the maturation rates of 0.14 and 0.6 mM treated groups were significantly lower than those of control (non-treated) group (P＜0.05). After 44h of culture in the same treatments of CySH, the GVBD rates of porcine immature oocytes were 90.0, 91.8, 89.8, 90.5 and 89.6%, respectively, and the maturation rates were 80.2, 76.3, 69.4, 66.7 and 72.6%, respectively. Especially, the maturation rates of 0.14 and 0.6 mM treated groups were significantly lower than those of control group (P＜0.05). 2. When the immature oocytes were cultured at 0, 0.05, 0.1, 0.5 and 1.0 mM of glutathione (GSH) for 36h, the GVBD rates of porcine immature oocytes were 91.0, 90.9, 89.5, 92.0 and 91.1%, respectively, and the maturation rates were 59.0, 48.5, 47.8, 38.6 and 37.5%, respectively. All treated groups of GSH showed lower maturation rates than the control group (P＜0.05). After 44h of culture in the same treatments of GSH, the GVBD rates of porcine immature oocytes were 91.8, 94.1, 89.1, 91.3 and 91.1%, respectively, and the maturation rates were 84.6, 57.1, 69.6, 71.3 and 64.3% respectively. All treated groups of GSH showed lower maturation rates than the control group (P＜0.05).

본 연구는 돼지 미성숙 난포란을 체외에서 성숙시킬 때, cysteine (CySH) 및 glutathione (GSH) 의 첨가 배양이 난핵포붕괴 및 핵성숙율에 미치는 영향을 조사하기 위하여 실시하였다. 본 연구에서 얻은 결과를 요약하면 다음과 같다. 1. 체외성숙 배양액인 TCM-199 에 cysteine (CySH) 을 각각 0, 0.04, 0.14, 0.6 및 1.2 mM 첨가하여 36 시간 동안 체외성숙을 유기시켰을 때의 난핵포붕괴율은 각각 90.8, 89.9, 90.5, 92.0 및 91.3% 으로서 각 처리구간 유의성이 없었고, 핵성숙율은 각각 56.1, 50.7, 41.9, 49.0 및 61.5%로서 대조구에 비하여 낮은 성숙율을 보였으며, 특히 0.14 mM 및 0.6 mM 첨가군에서 유의적으로 낮은 결과를 보였다 (P＜0.05). 또한, 44시간 동안 체외성숙을 유기시켰을 때 난핵포붕괴율은 각각 90.0, 91.8, 89.8, 90.5 및 89.6%로서 처리구간 유의성이 없었고, 핵성숙율은 각각 80.2, 76.3, 69.4, 66.7 및 72.6%로서 무첨가한 대조구에 비하여 비교적 낮은 성숙율을 보였으며, 특히 0.14 mM 및 0.6 mM 첨가군에서 유의적으로 낮은 결과를 보였다 (P＜0.05). 2. 체외성숙 배양액인 TCM-199 에 glutathione (GSH) 을 0, 0.05, 0.1, 0.5 및 1.0 mM 을 첨가하여 36시간 동안 성숙을 유기시켰을 때 난핵포 붕괴율은 각각 91.0, 90.9, 89.5, 92.0 및 91.1%로서 처리구간 유의성이 없이 높은 결과를 나타냈으며, 핵성숙율은 59.0, 48.5, 47.8, 38.6 및 37.5%로서 대조구에 비하여 모든 처리구에서 유의적으로 낮은 결과를 나타냈다 (P＜0.05). 또한, 44시간 동안 성숙을 유기시켰을 때 난핵포붕괴율은 각각 91.8, 94.1, 89.1, 91.3 및 91.1%로서 모든 처리구에서 높은 결과를 나타냈으며, 핵성숙율은 각각 84.6, 57.1, 69.6, 71.3 및 64.3%로서 대조구에 비하여 모든 처리구에서 유의적으로 낮은 결과를 나타냈다 (P＜0.05).