• 토지주택연구
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• 제3권1호
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• pp.79-82
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• 2012

While there exists a relatively large body of technical information for the engineered design of wood-frame buildings to resist seismic ground motions, the quantitative assessment of seismic resistance of conventional houses built by prescriptive requirements is less well understood. Forintek Canada Corp., in collaboration with other research and industry partners, has embarked on a research project to address this topic. This paper will report on the seismic shake table tests of a full-scale wood-frame building. The two-story specimen, $6m{\times}6m$ in plan, was built on the seismic shake table at Tongji University in Shanghai, China, according to Part 9 of the 1995 National Building Code of Canada and shaken uni-directionally in each of the two principal directions. Three different seismic table motions were applied at increasing peak ground motion amplitudes up to 0.40 and 0.50 g. The specimen was repaired after the above sets of seismic table motions, and successive runs were conducted for increased door openings. Measurements included specimen accelerations, displacements and anchorage forces. Static stiffness of the specimen was measured at low force levels, and natural frequencies were measured after each seismic loading stage by applying low-level random excitation. The results presented consist of the capacity spectra of the shake table tests, changes in specimen stiffness and natural frequencies with increasing seismic loading. These results and those from other recent shake table tests elsewhere will be compared with simplified engineering calculations based on codified values of strength, and on that basis preliminary conclusions will be drawn on the adequacy of the current code provisions and design guides in Canada and the USA for conventional wood-frame construction.

• Earthquakes and Structures
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• 제8권4호
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• pp.859-887
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• 2015

Structural health monitoring (SHM) has gained in popularity in recent years since it can assess the performance and condition of instrumented structures in real time and provide valuable information to the asset's manager and owner. Operational modal analysis plays an important role in SHM and it involves the determination of natural frequencies, damping ratios and mode shapes of a constructed structure based on measured dynamic data. This paper presents the operational modal analysis and seismic response characterization of the Tsing Ma Suspension Bridge of 2,160 m long subjected to different earthquake events. Three kinds of events, i.e., short-distance, middle-distance and long-distance earthquakes are taken into account. A fast Bayesian modal identification method is used to carry out the operational modal analysis. The modal properties of the bridge are identified and compared by use of the field monitoring data acquired before and after the earthquake for each type of the events. Research emphasis is given on identifying the predominant modes of the seismic responses in the deck during short-distance, middle-distance and long-distance earthquakes, respectively, and characterizing the response pattern of various structural portions (deck, towers, main cables, etc.) under different types of earthquakes. Since the bridge is over 2,000 m long, the seismic wave would arrive at the tower/anchorage basements of the two side spans at different time instants. The behaviors of structural dynamic responses on the Tsing Yi side span and on the Ma Wan side span under each type of the earthquake events are compared. The results obtained from this study would be beneficial to the seismic design of future long-span bridges to be built around Hong Kong (e.g., the Hong Kong-Zhuhai-Macau Bridge).

• 동의생리병리학회지
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• 제35권4호
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• pp.117-123
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• 2021

Gefitinib, a first generation epidermal growth factor receptor tyrosine kinase inhibitor (EGFR TKI), provides obvious clinical benefit in patients with EGFR-mutant non-small cell lung cancer (NSCLC). However, patients ultimately develop gefitinib resistance which mainly caused by EGFR T790M secondary mutation. In the current study, we investigated whether the root extract of Scutellaria baicalensis (SB) overcomes gefitinib resistance. Gefitinib-resistant H1975 human NSCLC cells (EGFR L858R/T790M double mutant) were treated with gefitinib and/or ethanol extract of SB (ESB) to evaluate the effect of ESB on the gefitinib sensitivity. The cell viability was measured by MTT assay and trypan blue exclusion assay. The colony-forming ability was evaluated by anchorage-dependent colony formation assay. Combined treatment with gefitinib and ESB markedly decreased the cell viability and colony formation than single treatment with gefitinib or ESB in H1975 cells. In addition, cells treated with both gefitinib and ESB exhibited a significant increase of sub-G1 DNA content which indicates apoptotic cells compared with those treated with gefitinib or ESB alone. As a molecular mechanism, combined treatment with gefitinib and ESB strongly downregulated the phosphorylation of ERK and JNK than single treatment with gefitinib or ESB. Taken together, our results demonstrate that ESB sensitizes H1975 cells to gefitinib treatment. We cautiously propose that ESB can be used in combination with gefitinib for the advanced NSCLC patients with acquired resistance to EGFR TKIs.

• Asian Pacific Journal of Cancer Prevention
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• 제16권6호
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• pp.2397-2402
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• 2015

Previous studies have shown that miR-454 plays an important role in a variety of biological processes in various human cancer cells. However, the underlying mechanisms of this microRNA in colorectal cancer (CRC) cells remain largely unknown. In the present study, we investigated the miR-454 role in CRC cell proliferation. We found that miR-454 expression is markedly upregulated in CRC tissues and CRC cells compared with the matched tumor adjacent tissues and the FHC normal colonic cell line. Ectopic expression of miR-454 promoted the proliferation and anchorage-independent growth of CRC cells, whereas inhibition of miR-454 reduced this effect. Bioinformatics analysis further revealed cylindromatosis (CYLD), a putative tumor suppressor as a potential target of miR-454. Data from luciferase reporter assays showed that miR-454 directly binds to the 3'-untranslated region (3'-UTR) of CYLD mRNA and repressed expression at both transcriptional and translational levels. In functional assays, CYLD-silenced in miR-454-in-transfected SW480 cells have positive effect to promote cell proliferation, suggesting that direct CYLD downregulation is required for miR-454-induced CRC cell proliferation. In sum, our data provide compelling evidence that miR-454 functions as an onco-miRNA, playing a crucial role in the promoting cell proliferation in CRC, and its oncogenic effect is mediated chiefly through direct suppression of CYLD expression.

• Earthquakes and Structures
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• 제18권1호
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• pp.113-127
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• 2020

In this study, the behavior of external beam-column joints reinforced by plain and deformed bars with non-seismic reinforcement details is investigated and compared. The beam-column joints represented in this study include a benchmark specimen by seismic details in accordance with ACI 318M-11 requirements and four other deficient specimens. The main defects of the non-seismic beam-column joints included use of plain bar, absence of transverse steel hoops, and the anchorage condition of longitudinal reinforcements. The experimental results indicate that using of plain bars in non-seismic beam-column joints has significantly affected the failure modes. The main failure mode of the non-seismic beam-column joints reinforced by deformed bars was the accumulation of shear cracks in the joint region, while the failure mode of the non-seismic beam-column joints reinforced by plain bars was deep cracks at the joint face and intersection of beam and column and there was only miner diagonal shear cracking at the joint region. In the other way, use of plain bars for reinforcing concrete can cause the behavior of the substructure to be controlled by slip of the beam longitudinal bars. The experimental results show that the ductility of non-seismic beam-column joints reinforced by plain bars has not decreased compared to the beam-column joints reinforced by deformed bars due to lack of mechanical interlock between plain bars and concrete. Also it can be seen a little increase in ductility of substructure due to existence of hooks at the end of the development length of the bars.

• Smart Structures and Systems
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• 제23권6호
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• pp.565-577
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• 2019

One of the most effective countermeasures for mitigating cable vibration is to install mechanical dampers near the anchorage of the cable. Most of the dampers used in the field are so-called passive dampers where their parameters cannot be changed once designed. The parameters of passive dampers are usually determined based on the optimal damper force obtained from the universal design curve for linear dampers, which will provide a maximum additional damping for the cable. As the optimal damper force is chosen based on a predetermined principal vibration mode, passive dampers will be most effective if cable undergoes single-mode vibration where the vibration mode is the same as the principal mode used in the design. However, in the actual engineering practice, multi-mode vibrations are often observed for cables. Therefore, it is desirable to have dampers that can suppress different modes of cable vibrations simultaneously. In this paper, MR dampers are proposed for controlling multi-mode cable vibrations, because of its ability to change parameters and its adaptability of active control without inquiring large power resources. Although the highly nonlinear feature of the MR material leads to a relatively complex representation of its mathematical model, effective control strategies can still be derived for suppressing multi-mode cable vibrations based on nonlinear modelling, as proposed in this paper. Firstly, the nonlinear Bouc-wen model is employed to accurately portray the salient characteristics of the MR damper. Then, the desired optimal damper force is determined from the universal design curve of friction dampers. Finally, the input voltage (current) of MR damper corresponding to the desired optimal damper force is calculated from the nonlinear Bouc-wen model of the damper using a piecewise linear interpolation scheme. Numerical simulations are carried out to validate the effectiveness of the proposed control algorithm for mitigating multi-mode cable vibrations induced by different external excitations.

• Computers and Concrete
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• 제29권 6호
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• pp.375-391
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• 2022

This paper examined the impact of the cross-sectional structure on the structural results under different loading conditions of reinforced concrete (RC) members' management limited in Carbon Fiber Reinforced Polymers (CFRP). The mechanical properties of CFRC was investigated, then, totally 32 samples were examined. Test parameters included the cross-sectional shape as square, rectangular and circular with two various aspect rates and loading statues. The loading involved concentrated loading, eccentric loading with a ratio of 0.46 to 0.6 and pure bending. The results of the test revealed that the CFRP increased ductility and load during concentrated processing. A cross sectional shape from 23 to 44 percent was increased in load capacity and from 250 to 350 percent increase in axial deformation in rectangular and circular sections respectively, affecting greatly the accomplishment of load capacity and ductility of the concentrated members. Two Artificial Intelligence Models as Extreme Learning Machine (ELM) and Particle Swarm Optimization (PSO) were used to estimating the tensile and flexural strength of specimen. On the basis of the performance from RMSE and RSQR, C-Shape CFRC was greater tensile and flexural strength than any other FRP composite design. Because of the mechanical anchorage into the matrix, C-shaped CFRCC was noted to have greater fiber-matrix interfacial adhesive strength. However, with the increase of the aspect ratio and fiber volume fraction, the compressive strength of CFRCC was reduced. This possibly was due to the fact that during the blending of each fiber, the volume of air input was increased. In addition, by adding silica fumed to composites, the tensile and flexural strength of CFRCC is greatly improved.

• Biomolecules & Therapeutics
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• 제31권2호
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• pp.200-209
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• 2023

Patients with non-small-cell lung cancer (NSCLC) with epidermal growth factor receptor (EGFR) amplification or sensitive mutations initially respond to the tyrosine kinase inhibitor gefitinib, however, the treatment becomes less effective over time by resistance mechanism including mesenchymal-epithelial transition (MET) overexpression. A therapeutic strategy targeting MET and EGFR may be a means to overcoming resistance to gefitinib. In the present study, we found that picropodophyllotoxin (PPT), derived from the roots of Podophyllum hexandrum, inhibited both EGFR and MET in NSCLC cells. The antitumor efficacy of PPT in gefitinib-resistant NSCLC cells (HCC827GR), was confirmed by suppression of cell proliferation and anchorage-independent colony growth. In the targeting of EGFR and MET, PPT bound with EGFR and MET, ex vivo, and blocked both kinases activity. The binding sites between PPT and EGFR or MET in the computational docking model were predicted at Gly772/Met769 and Arg1086/Tyr1230 of each ATP-binding pocket, respectively. PPT treatment of HCC827GR cells increased the number of annexin V-positive and subG1 cells. PPT also caused G2/M cell-cycle arrest together with related protein regulation. The inhibition of EGFR and MET by PPT treatment led to decreases in the phosphorylation of the downstream-proteins, AKT and ERK. In addition, PPT induced reactive oxygen species (ROS) production and GRP78, CHOP, DR5, and DR4 expression, mitochondrial dysfunction, and regulated involving signal-proteins. Taken together, PPT alleviated gefitinib-resistant NSCLC cell growth and induced apoptosis by reducing EGFR and MET activity. Therefore, our results suggest that PPT can be a promising therapeutic agent for gefitinib-resistant NSCLC.

• Molecules and Cells
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• 제38권4호
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• pp.373-379
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• 2015

Pyruvate kinase M2 isoform (PKM2), a rate-limiting enzyme in the final step of glycolysis, is known to be associated with the metabolic rewiring of cancer cells, and considered an important cancer therapeutic target. Herein, we report a novel PKM2 activator, PA-12, which was identified via the molecular docking-based virtual screening. We demonstrate that PA-12 stimulates the pyruvate kinase activity of recombinant PKM2 in vitro, with a half-maximal activity concentration of $4.92{\mu}M$, and effectively suppresses both anchorage-dependent and -independent growth of lung cancer cells in non-essential amino acid-depleted medium. In addition, PA-12 blocked the nuclear translocalization of PKM2 in lung cancer cells, resulting in the inhibition of hypoxia response element (HRE)-mediated reporter activity as well as hypoxia-inducible factor 1 (HIF-1) target gene expression, eventually leading to the suppression of cell viability under hypoxia. We also verified that the effects of PA-12 were dependent on PKM2 expression in cancer cells, demonstrating the specificity of PA-12 for PKM2 protein. Taken together, our data suggest that PA-12 is a novel and potent PKM2 activator that has therapeutic implications for lung cancer.

• 해양환경안전학회지
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• 제26권7호
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• pp.904-914
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• 2020

우리나라는 산업통상자원부를 통해 'LNG 벙커링(연료공급) 전용선 건조지원사업'을 한국가스공사를 중심으로 추진하고 있고, 동시 해양수산부는 부산신항내 LNG 벙커링 터미널 구축을 추진하고 있다. LNG 벙커링 전용선박은 LNG 연료를 터미널에서 대상 선박으로 공급하기 위한 필수 대상이고, 이에 따라 안정 운항에 대한 절차서의 필요성이 대두되고 있다. 따라서 본 연구는 연안선박용 LNG벙커링 전용선박의 운항 절차서를 제안하기 위하여 부산 신항에서 부산항 정박지로 운항하는 연안선박용 LNG 벙커링 전용선박의 안정성을 평가하였다. 이를 위해 포텐셜 유동해석 기반의 운동해석을 수행하여 취득한 선체운동 응답진폭함수를 운항 해역의 5년간 관측된 실해역 자료와 부산 연안에 적합한 TMA스펙트럼과 합성하여 유의파고별 연안선박용 LNG 벙커링 전용 선박의 내항성능 평가를 수행하였다. 그 결과는 유의파고 2 m 이상에서 횡동요와 수평가속도가 내항성능에 주요 위험 요소가 됨을 알 수 있었다. LNG 벙커링 전용선박의 운항 가능 기간은 총 관측기간의 83.3 ~ 99.9 % 수준임을 알 수 있었다.