• Structural Engineering and Mechanics
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• 제24권4호
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• pp.463-478
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• 2006

The design of structural frameworks for buildings is constantly evolving and is dependent on regional issues such as loading and constructability. One of the most promising recent developments for low to medium rise construction in terms of efficiency of construction, robustness and aesthetic appearance utilises concrete-filled steel tubular sections as the columns in a moment-resisting frame. These are coupled to rigid or semi-rigid connections to composite steel-concrete beams. This paper includes the results of a pilot experimental programme leading towards the development of economical, reliable connections that are easily constructed for this type of frame. The connections must provide the requisite strength, stiffness and ductility to suit gravity loading conditions as well as gravity combined with the governing lateral wind or earthquake loading. The aim is to develop connections that are stiffer, less expensive and easier to construct than those in current use. A proposed fabricated T-stub connection is to be used to connect the beam flanges and the column. These T-stubs are connected to the column using "blind bolts" with extensions, allowing installation from the outside of the tube. In general, the use of the extensions results in a dramatic increase in the strength and stiffness of the T-stub to column connection in tension, since the load is shared between membrane action in the tube wall and the anchorage of the bolts through the extensions into the concrete.

• Earthquakes and Structures
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• 제19권5호
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• pp.315-329
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• 2020

Through bolt connections in Concrete Filled Steel Tubes (CFSTs) has been proved to be good in terms of seismic performance and constructability. Stiffened extended end plate connection with full through type bolt helps to avoid field weld altogether, and hence to improve the quality of joints. An experimental study was conducted on the hysteretic performance of square interior beam-column connections using flat extended end plates with through bolt. The study focuses on the effect of the bond between the tie rod and the core concrete on the cyclic performance of the joint. The study also quantifies how much the interior joint is getting strengthened due to the confinement effect induced by bi-directional bolting, which is widely used in 3D moment resisting frames. For a better understanding of the mechanism and for the prediction of shear capacity of the panel zone, a mathematical model was generated. The various parameters included in the model are the influence of axial load, amount of prestress induced by bolt tightening, anchorage, and the concrete strut action. The study investigates the strength, stiffness, ductility, and energy dissipation characteristics. The results indicate that the seismic resistance is at par with American Institute of Steel Construction (AISC) seismic recommendations. The bidirectional bolting and bond effect have got remarkable influence on the performance of joints.

• BMB Reports
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• 제47권7호
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• pp.411-416
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• 2014

In the present study, we demonstrate that ectopic expression of 56-kDa human selenium binding protein-1 (hSP56) in PC-3 cells that do not normally express hSP56 results in a marked inhibition of cell growth in vitro and in vivo. Down-regulation of hSP56 in LNCaP cells that normally express hSP56 results in enhanced anchorage-independent growth. PC-3 cells expressing hSP56 exhibit a significant reduction of hypoxia inducible protein (HIF)-$1{\alpha}$ protein levels under hypoxic conditions without altering HIF-$1{\alpha}$ mRNA (HIF1A) levels. Taken together, our findings strongly suggest that hSP56 plays a critical role in prostate cells by mechanisms including negative regulation of HIF-$1{\alpha}$, thus identifying hSP56 as a candidate anti-oncogene product.

• Earthquakes and Structures
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• 제14권6호
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• pp.493-503
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• 2018

With the further increase of span length, the cable-stayed bridge tends to be more slender, and becomes more susceptible to the seismic action. By taking a super long-span cable-stayed bridge with main span of 1400m as example, structural response of the bridge under the E1 horizontal and vertical seismic excitations is investigated numerically by the multimode seismic response spectrum and time-history analysis respectively, the seismic behavior and also the effect of structural nonlinearity on the seismic response of super long-span cable-stayed bridge are revealed. Furthermore, the effect of structural parameters including the girder depth and width, the tower structural style, the tower height-to-span ratio, the side-tomain span ratio, the auxiliary piers in side spans and the anchorage system of stay cables etc on the seismic performance of super long-span cable-stayed bridge is investigated numerically by the multimode seismic response spectrum analysis, and the favorable earthquake-resistant structural system of super long-span cable-stayed bridge is proposed.

• Wind and Structures
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• 제32권5호
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• pp.405-421
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• 2021

To gain insight into the wind-induced safety concerns associated with attached tower cranes during the construction of super-tall buildings, a 606 m level frame-core tube super-tall building is selected to investigate the wind-induced vibration response and fragility of an outer-attached tower crane at all stages of construction. The wind velocity time history samples are artificially generated and used to perform dynamic response analyses of the crane to observe the effects of wind velocity and wind direction under its working and non-working resting state. The adverse effects of the relative displacement response at different connection supports are also identified. The wind-resistant fragility curves of the crane are obtained by introducing the concept of incremental dynamic analysis. The results from the investigation indicate that a large relative displacement between the supports can substantially amplify the response of the crane at high levels. Such an effect becomes more serious when the lifting arm is perpendicular to the plane of the connection supports. The flexibility of super-tall buildings should be considered in the design of outer-attached tower cranes, especially for anchorage systems. Fragility analysis can be used to specify the maximum appropriate height of the tower crane for each performance level.

• Geomechanics and Engineering
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• 제37권1호
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• pp.31-48
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• 2024

To stabilize the excavations in urban area, soil anchorage is among the very common methods in geotechnical engineering. A more efficient deformation analysis can potentially lead to cost-effective and safer designs. To this end, a total of 116 three-dimensional (3D) finite element (FE) models of a deep excavation supported by tie-back wall system were analyzed in this study. An initial validation was conducted through examination of the results against the Texas A&M excavation cases. After the validation step, an extensive parametric study was carried out to cover significant design parameters of tie-back wall system in deep excavations. The numerical results indicated that the maximum horizontal displacement values of the wall (δ_hm) and maximum surface settlement (δ_vm) increase by an increase in the value of ground anchors inclination relative to the horizon. Additionally, a change in the wall embedment depth was found to be contributing more to δ_vm than to δ_hm. Based on the 3D FE analysis results, two simple equations are proposed to estimate excavation deformations for different scenarios in which the geometric configuration parameters are taken into account. The model proposed in this study can help the engineers to have a better understanding of the behavior of such systems.

• 대한치과교정학회지
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• 제36권5호
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• pp.339-348
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• 2006

교정용 미니 임플랜트 고정원을 이용한 교정 치료가 보편화되며, SWA와 이를 이용한 on masse sliding retraction 은 임상에서 흔히 사용하는 치료법이 되었다. 그러나 고정원을 성공적으로 보존하려는 노력에 비해, 발치 공간 폐쇄시 전치부 치축 조절에 관여하는 요인에 대한 보고는 아직까지 부족한 실정이다. 본 연구에서는 제1소구치를 제거한 상악 치아와 치주 인대 그리고 치조골에 대한 3차원 유한요소 기준모델을 제작하였고, 제1대구치와 제2소구치 사이 주호선 10 mm 상방에 식립된 교정용 미니 임플랜트를 고정원으로 사용할 경우, 측절치-견치 사이의 견인 훅의 높이를 변화시키며 후상방 견인력을 가하거나, 주호선에 보상 만곡을 부여하는 것이 전치부 치축 조절에 어떤 영향을 미치는지 시뮬레이션 하였다. 또한 전치부 치축이 설측 경사된 모델을 같은 실험 조건으로 시뮬레이션 하여 발치 공간 페쇄 시 설측 경사된 전치부 치축을 유지하거나 개선할 수 있는 요인을 검토하였고, 다음과 같은 연구 결과를 얻었다. 2 mm 높이의 견인 훅에 대하여 후상방으로 견인력을 가할 경우 발생하는 함입력으로 인하여 전치부 설측 경사가 더 감소되지는 않았다. 견인 훅의 높이가 5 mm인 경우 후상방 견인력을 가하면, 측절치의 치관 순측 및 치근 설측 이동이 일어나고, 견치의 비조절성 후방 경사 이동이 심화되었다. 4 mm의 보상 만곡은 측절치의 치관 순측 및 치근 설측 이동을 일으키고, 견치의 비조절성 후방 경사 이동을 감소시켰다. 또한 전치부가 설측 경사된 모델을 기준모델과 같은 실험 조건으로 시뮬레이션 한 경우 치근면의 응력 분포와 25000배 확대된 그래프 상에서의 치아 이동 양상은 매우 유사하였다 이상의 결과는 미니 임플랜트-SWA sliding 생역학을 구사 시 견인 훅의 위치와 와이어 상의 보상 만곡의 유무에 의해 전치부의 치축 조절이 달라지며 실제 임상에서 가이드라인으로 활용될 수 있을 것이다. 따라 수용자들의 적극적 인식도 심화되었다고 결론지을 수 있을 것이다. 신문이 일제의 지배방식에 순응해 독자들에게 내선일체와 전쟁협력을 강요했다는 역사적 평가를 듣게 만들었다.사되었으며 그 다음은 근 현대가 18편으로 26.47%, 중세 7편 10.3%, 선사시대 5편 7.35%, 상고시대가 1편으로 1.47%로 나타나 고대 중세, 근 현대 순으로 선호하는 것으로 나타났다.기인계 농약의 특징인 불안정성에 의해 광분해 및 미생물 분해를 통해 이미 분해가 진행 중이어서 본래의 유기인 화합물이 검출되지 않았을 가능성이 있다. 그리고 유기인계 농약이 완전히 분해되어 생성된 유기인계 농약 기원의 영양염류가 저수지 내로 많은 양이 유입된다면, 부영양화에 기여할 가능성이 있다. 따라서 농업용 저수지에서 유기인계 농약의 농도 분포는 물론이고 중간 생성물과 그 독성, 광분해 및 미생물 분해의 메커니즘, 그리고 최종 산물에 대한 연구가 필요하다.삭감율은 BOD의 경우 Scenario 1-1(처리용량 1,500 $m^3$ $day^{-1}$인 인공습지), scenario 1-2 (처리용량 1,000 $m^3$ $day^{-1}$인 인공습지), scenario 2(면적 4.2ha인 저류지)가 각각 연평균 6.9%, 4.8%, 7.1%의 감소를 보였다. TN은 4.7%, 3.4%, 13.4%의 삭감율을 나타내었으며, TP는 5.6%, 3.9%, 7.3%의 삭감율을 나타내었다. 본 연구에서는 적용하지 못하였으나, 인공습지와 저류지의 적절한 연계시스템을 적용한다면 저감시설 설치 부지면적과 비용의 감소뿐만 아니라 보다 효과적인 수질개선효과를 가져올 수 있으리라 판단된다.다. 이상과 같이 조선시대 주식류의 종류 및 조리방법에 대한 문헌적 고찰을 분석한 결과로 조선시대로부터 현재까지 주식류의 변천과정을 파악할 수 있었으며 새롭게 문헌으로라도 복원된 전통음식인

• 대한한방내과학회지
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• 제29권2호
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• pp.490-499
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• 2008

Objectives : This study was aimed to elucidate the effects of Trichosanthes kirilowii extract (TKE) on the angiogenesis and growth of tumor cells. Methods : Tube formation assay was performed by using human umbilical vein endothelial cells (HUVEC), and anchorage dependent colony assay was performed by using B16-F10 melanoma, Hep G2 and HT1080, CT-26 and SNU-1 cells. Results : For HUVEC, TKE at a level of more than 100 ${\mu}g/m{\ell}$ suppresses cell growth. For HUVEC at 100 ${\mu}g/m{\ell}$ and greater TKE density, the formation of tubes was suppressed in a dose-dependant manner. TKE controls the colony formations of B16-F10 melanoma cells, CT 26 cells, and Hep G2 cells, and its effect is proportional to density. In HT1080 cells and SNU-1 cells, formation is suppressed regardless of density. Conclusions : From these results, it could be concluded that TKE has significant properties on anti-angiogenesis and growth inhibiting of tumor cells. It is suggested that TKE will be a good candidate for new drugs or therapeutics for anti-angiogenesis.

• Journal of Microbiology and Biotechnology
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• 제23권9호
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• pp.1308-1321
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• 2013

The emergence of microcarrier technology has brought a renewed interest in anchorage-dependent cell culture for high-yield processes. Well-known in vaccine production, microcarrier culture also has potential for application in other fields. In this work, two types of microcarriers were evaluated for small-scale monoclonal antibody (mAb) production by CHO-K1 cells. Cultures (5 ml) of microporous Cytodex 3 and macroporous CultiSpher-S carriers were performed in vented conical tubes and subsequently scaled-up (20 ml) to shake-flasks, testing combinations of different culture conditions (cell concentration, microcarrier concentration, rocking methodology, rocking speed, and initial culture volume). Culture performance was evaluated by considering the mAb production and cell growth at the phases of initial adhesion and proliferation. The best culture performances were obtained with Cytodex 3, regarding cell proliferation (average $1.85{\pm}0.11{\times}10^6$ cells/ml against $0.60{\pm}0.08{\times}10^6$ cells/ml for CultiSpher-S), mAb production ($2.04{\pm}0.41{\mu}g/ml$ against $0.99{\pm}0.35{\mu}g/ml$ for CultiSpher-S), and culture longevity (30 days against 10-15 days for CultiSpher-S), probably due to the collagen-coated dextran matrix that potentiates adhesion and prevents detachment. The culture conditions of greater influence were rocking mechanism (Cytodex 3, pulse followed by continuous) and initial cell concentration (CultiSpher-S, $4{\times}10^5$ cells/ml). Microcarriers proved to be a viable and favorable alternative to standard adherent and suspended cultures for mAb production by CHO-K1 cells, with simple operation, easy scale-up, and significantly higher levels of mAb production. However, variations of microcarrier culture performance in different vessels reiterate the need for optimization at each step of the scale-up process.

• 한국구조물진단유지관리공학회 논문집
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• 제22권3호
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• pp.97-102
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• 2018

기존 PSC I형 거더는 콘크리트의 자중, 정착구 및 긴장방식 등의 영향으로 장경간화의 적용성이 불리하였다. 이를 극복하기 위하여 PSC 거더의 복부에 중공을 도입하고 다단계 긴장을 도입함으로써 50~70m 경간에 적용 가능한 중공 웨브 PSC I형 거더를 개발하고 실교량으로 시공하였다. 본 연구는 중공 웨브 PSC I형 거더교 현장에서 정적재하시험을 통하여 계측을 한 결과와 대상 구조물의 유한요소해석 결과를 바탕으로 비교, 분석하여 중공 웨브 장경간 PSC 거더교의 공용내하력과 안전성을 평가하였다. 본 교량의 정적재하시험과 수치해석 결과가 유사하게 나타났으며 중공 웨브 PSC I형 거더의 거동을 잘 모사하는 것으로 나타났다. 교량의 모든 거더는 설계 활하중 하에서 충분한 내하력을 확보하는 것으로 평가되었고 안전성을 확보하여 시공 결과의 적절성을 확인하였다.