• Plant Biotechnology Reports
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• v.4 no.4
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• pp.321-328
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• 2010

An efficient protocol for in vitro organogenesis was achieved from callus-derived immature and mature leaf explants of Momordica charantia, a very important vegetable and medicinal plant. Calluses were induced from immature leaf explants excised from in vitro (15-day-old seedlings) mature leaf explants of vivo plants (45 days old). The explants were grown on Murashige and Skoog (MS) medium with Gamborg (B5) vitamins containing 30 g $1^{-1}$ sucrose, 2.2 g $1^{-1}$ Gelrite, and 7.7 lM naphthalene acetic acid (NAA) with 2.2 ${\mu}M$ thidiazuron (TDZ). Regeneration of adventitious shoots from callus (30-40 shoots per explant) was achieved on MS medium containing 5.5 ${\mu}M$ TDZ, 2.2 ${\mu}M$ NAA, and 3.3 ${\mu}M$ silver nitrate ($AgNO_3$). The shoots (1.0 cm length) were excised from callus and elongated in MS medium fortified with 3.5 ${\mu}M$ gibberellic acid ($GA_3$). The elongated shoots were rooted in MS medium supplemented with 4.0 ${\mu}M$ indole 3-butyric acid (IBA). Rooted plants were acclimatized in the greenhouse and subsequently established in soil with a survival rate of 90%. This protocol yielded an average of 40 plants per leaf explant with a culture period of 98 days.

• BMB Reports
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• v.39 no.2
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• pp.216-222
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• 2006

In the present study, we compared six different solubilization buffers and optimized two-dimensional electrophoresis (2-DE) conditions for human lymph node proteins. In addition, we developed a simple protocol for 2-D gel storage. Efficient solubilization was obtained with lysis buffers containing (a) 8M urea, 4% CHAPS (3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate), 40 mM Tris base, 65 mM DTT(dithiothreitol) and 0.2% carrier ampholytes; (b) 5M urea, 2M thiourea, 2% CHAPS, 2% SB 3-10 (N-decyl-N, N-dimethyl-3-ammonio-1-propanesulfonate), 40mM Tris base, 65 mM DTT and 0.2% carrier ampholytes or (c) 7M urea, 2M thiourea, 4% CHAPS, 65 mM DTT and 0.2% carrier ampholytes. The optimal protocol for isoelectric focusing (IEF) was accumulated voltage of 16,500 Vh and 0.6% DTT in the rehydration solution. In the experiments conducted for the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), best results were obtained with a doubled concentration (50 mM Tris, 384 mM glycine, 0.2% SDS) of the SDS electrophoresis buffer in the cathodic reservoir as compared to the concentration in the anodic reservoir (25 mM Tris, 192 mM glycine, 0.1% SDS). Among the five protocols tested for gel storing, success was attained when the gels were stored in plastic bags with 50% glycerol. This is the first report describing the successful solubilization and 2D-electrophoresis of proteins from human lymph node tissue and a 2-D gel storage protocol for easy gel handling before mass spectrometry (MS) analysis.

• Proceedings of the Korea Information Processing Society Conference
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• 2011.04a
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• pp.572-575
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• 2011

It is well known that spanning tree protocol (STP) is the most commonly used protocol in switching networks for smart grid. STP selectively blocks redundancy links of the network to prevent layer 2 loops in network, and it also has a functionality of backing up links. As with the other protocols, STP has been updated with the continuing development of the network. STP is a broad concept and it does not just refer in particular to defined STP protocol in IEEE 802.1D standards, it refers to updated spanning tree protocol based on STP. Because of uneven distribution of communication traffic in root bridge, STP cannot satisfy fast converge nce while the failure occurs near the root bridge or on the root bridge in tree topologies of STP. In this paper, we propose a novel method --- Hyper Cube Spanning Tree Protocol (HCSTP) to solve uneven distribution of communication traffic. Theoretically, hyper cube in our protocol increases throughput and improves the utilization of communication. The simulation results show that HCSTP can achieve comparative and considerably higher performance than other STP protocols in terms of reconnection.

• 한국IT서비스학회:학술대회논문집
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• 2006.05a
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• pp.181-185
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• 2006

최근 차량에 설치된 단말기를 통해 길안내 서비스, 교통정보 서비스 등 다양한 정보를 제공하는 텔레매틱스 서비스가 활발하게 연구되고 있다. 하지만 현재 대다수의 텔레매틱스 서비스는 최대 15m 의 공간오차를 가진 GPS 기술을 이용하기 때문에 차량의 정확한 위치정보를 파악하기는 힘들다. 따라서 본 논문에서는 무선 센서노드를 이용해 GPS 보다 정확한 차량의 위치정보와 속도를 감지하고 노드 간 통신 프로토콜로서 Telematics Scheduling Protocol(TSP)[1]을 사용하여 교차로 중앙에 위치한 베이스 스테이션으로 전송하여 교차로에서 발생할 수 있는 차량 충돌을 예측하며 충돌 위험 정보를 교차로에 근접한 자동차에게 알려주는 텔레매틱스 교통안전시스템[1]을 소개한다. 또한 교통안전시스템으로서의 신뢰성과 실시간성을 비교평가하기 위하여 TSP 프로토콜, IEEE 802.11[2]과 802.15.4[3]를 대상으로 네트워크 시뮬레이터 ns-2[4]를 이용하여 시뮬레이션한다.

• The Journal of the Korea institute of electronic communication sciences
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• v.12 no.1
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• pp.47-52
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• 2017

This study is BIT(: Bus Information Terminal) features that take advantage of KEPCO eIoT(: energy Internet of Thing) platform, and it's Network configuration is composed of display terminal device, gateway, platform, and the service server. The key features are parts for processing protocol data between the gateway and the device using LoRa(: Long Range) technology, Intelligent applications and SIP(: Session Initiation Protocol) data handling connected to the Taxi reservation system. And the resource tree provided BIT for the service, which commonly used in the application server and the device.

• Molecules and Cells
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• v.24 no.1
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• pp.76-82
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• 2007

By combining in situ hybridization (ISH) and immunocytochemistry (IC), microscopic topological localization of mRNAs and proteins can be determined. Although this technique can be applied to a variety of tissues, it is particularly important for use on neuronal cells which are morphologically complex and in which specific mRNAs and proteins are located in distinct subcellular domains such as dendrites and dendritic spines. One common technical problem for combined ISH and IC is that the signal for immunocytochemical localization of proteins often becomes much weaker after conducting ISH. In this manuscript, we report a simplified but robust protocol that allows immunocytochemical localization of proteins after ISH. In this protocol, we fix cultured cortical or hippocampal neurons with 4% paraformaldehyde (PFA), rinse briefly in PBS, and then further fix the cells with $-20^{\circ}C$ methanol. Our method has several major advantages over previously described ones in that (1) it is simple, as it is just consecutive routine fixation procedures, (2) it does not require any special alteration to the fixation procedures such as changes in salt concentration, and (3) it can be used with antibodies that are compatible with either methanol (MeOH-) or PFA-fixed target proteins. To our best knowledge, we are the first to employ this fixation method for fluorescence ISH + IC.

• Journal of Plant Biotechnology
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• v.48 no.2
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• pp.93-99
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• 2021

This study was conducted to develop an Agrobacterium-mediated genetic transformation protocol for the carnation cv. "Jinju" to counteract its ethylene sensitivity. The new protocol involves the use of an improved shoot regeneration medium, optimized minimal concentrations of the selective agent, a pre-culture period, and co-cultivation periods. Silver nanoparticles (NAg) added at a concentration of 2.0 μM to the Murashige and Skoog (MS) basal shoot regeneration medium supplemented with 0.1 mg/L indole-3-butyric-acid (IBA) and 0.2 mg/L thidiazuron (TDZ) improved the shoot regeneration efficiency, number of shoots per explant, and plant growth compared to the control without the addition of NAg. The phosphinothricin (PPT) concentration of 1.0 mg/L was determined to be the minimal and optimal concentration for the selection of putative transgenic plants. When the explants were infected with Agrobacterium cells harboring the acdS gene, the explants that were pre-cultured for three days induced more putative transgenic plants than those that were co-cultivated for four days. Therefore, we expect that the results of this study will benefit researchers who are developing genetic transformations of carnations.

• Journal of Forest and Environmental Science
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• v.27 no.1
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• pp.39-46
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• 2011

This study purposed to analyze the spatial pattern and the amount of carbon emission at the deforestation area in Gangwondo. Forest geographic information system(FGIS) and administrative data were used in the analysis. The area size and spatial patterns of deforestation area were analyzed according to the article 3.3 of Kyoto protocol. Forest administration data for 9 years from 2000 to 2008 were entered into a database. Fifty-nine percent of deforestation area was found within 200m of the road network, and seventy-five percent of the area was found within 500m. Theoretical carbon emission based on deforestation area was estimated at 6,968tc. Carbon emission of national forest was 5.7times higher than that of private forest.

• Journal of Plant Biotechnology
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• v.6 no.4
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• pp.221-227
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• 2004

Protocol was established for production of virus free healthy seeds using meristem ($0.3-0.5\;\cal{mm}$ in size) culture and field management under net house condition in tomato. The isolated meristem was found well established in MS liquid medium containing $0.1\;\cal{mg}\;1^{-1}\;of\;GA_3$. For shoot and root development either from primary meristem or from nodal segment of meristem derived plants, semisolid MS medium having $0.5\;\cal{mg}\;1^{-1}$ of IBA was found most effective. The elimination of the studied viruses (ToMV, CMV, ToLCV) in meristem-derived plants was confirmed by DAS-ELISA test. For field management of the virus eradicated meristem-derived plants, use of net house was found very effective measures to check viral vector visit and eventually infection. The meristem-derived plants were vigor and high yielder than the native seed derived plants and produced healthy seeds. Due to stop vector visit, no viral symptoms were observed in both $R_1\;and\;R_2$ plants cultivated in net house condition. Starting of viral infestation was observed in $R_2$ generation when they were planted in open house condition without control of vector visit. Therefore, for management of viral diseases, use of virus free meristem derived plantlets and their subsequent cultivation in soil under net house condition without using any vector killing insecticide can be recommended for producing healthy seeds in tomato. The developed protocol for environmentally healthy tomato seed production in Bangladesh may be used in the countries having similar tropical like environment conducive for viral vector visit.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.16 no.5
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• pp.906-912
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• 2012

A ship ad-hoc network (SANET) can provide ships with diverse multimedia services by replacing expensive satellite communications. While ITU-R M. 1842-1, standards for maritime VHF band digital communications, can be used as the specifications of physical layer for SANETs, no standards are specified for higher layers of SANETs. In this paper, we propose a ship ad-hoc communication (SAC) protocol for SANETs, based on medium access control (MAC) and routing protocols for terrestrial ad-hoc networks. SAC protocol is a cross-layer protocol which combines MAC and routing into one algorithm and considers maritime environments, including the existence of neighboring ships, the possibility of routing to a destination, and changing the communication mode in case of VHF channel failure.