• 한국수산과학회지
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• 제50권3호
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• pp.302-310
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• 2017

Interferon regulatory factor 8 (IRF8) is essential for the development of B and T cells, as well as for the activity of dendritic cells and macrophages. We performed molecular characterization of IRF8 from rock fish, Sebastes schlegelii (Ss), and investigated the spatial and temporal profile of mRNA expression after challenge with lipopolysaccharide (LPS), polyinosinic:polycytidylic acid (poly I:C), or Streptococcus iniae. The full-length cDNA sequence of SsIRF8 was 1,657 bp, containing an ORF of 1,266 bp. The gene had a predicted molecular mass of 47.7 kDa and an isoelectric point of 5.99. The amino acid sequence coded by this gene showed the highest degree of identity (90.8%) and similarity (96.2%) with IRF8 from Oplegnathus fasciatus. The SsIRF8 mRNA was expressed ubiquitously, at varying levels, with the highest level of expression observed in the spleen. To confirm the role of SsIRF8 in mediating the immune response, we measured SsIRF8 mRNA expression in the splenic tissue at different time points after injection with LPS, poly I:C, or S. iniae. The qRT-PCR results showed that SsIRF8 mRNA expression in the poly I:C-injected group was highly upregulated 6 hr after exposure (P<0.05). Expression of SsIRF8 mRNA in the S. iniae-injected group peaked at 24 hr. These results suggest that SsIRF8 might be important in regulating the strength of the rockfish immune response to immunostimulatory agents.

• 자원리싸이클링
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• 제26권3호
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• pp.53-60
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• 2017

블랙드로스에는 금속 알루미늄, 알루미나, 실리카, 산화마그네슘, 가용성 염 및 미량 성분이 함유되어 있다. 블랙드로스를 사용가능한 재료로 전환시키기 위해서는 실리카의 양을 조절하는 것이 중요하다. 먼저 가용성 염인 염화나트륨과 염화칼륨은 $50^{\circ}C$에서 물에 용해되었다. 물세척 후 잔사에 함유된 실리카, 알루미나, 산화마그네슘 및 산화타이타늄의 침출거동을 NaOH의 농도와 반응온도를 변화시키며 조사하였다. 반응온도 $25{\sim}95^{\circ}C$에서 알루미나의 침출율은 온도에 비례하나 실리카의 침출의 경우에는 최적 온도가 존재하였다. 한편 2~6 M의 NaOH용액에 산화마그네슘은 전혀 용해되지 않았다. 5 M의 NaOH와 $95^{\circ}C$에서 알루미나와 실리카의 침출율은 각각 80과 68%이었다.

• 상하수도학회지
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• 제31권5호
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• pp.447-457
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• 2017

This study derived the unit of industrial water usage reflecting the latest industry trends. Available for establishing plans such as the master plan for water supply system and analyzed changes in the basic unit by a comparison with the current basic unit values. This study analyzed 4,038 samples with a sampling error of less than 1.5 % at the 95 % confidence level after removing outliers according to a log-normal distribution. As a result, the unit of industrial water usage per site area in the whole manufacturing industry was 7.11 m3/1,000m2/d. The ten industrial categories (C10, C13, C20, C21, C22, C25, C27, C30, C32, C33) showed a similar unit value compared to before, and the four industrials categories (C11, C17, C22, C31) showed a more unit value than before. With regard to the nine industrial categories (C14, C15, C16, C18, C19, C24, C26, C28, C29), the unit value decreased. Cases that companies examined before were the same as the companies examined in this study were analyzed. The result that the changes in the unit industrial water usage were reasonable was obtained. However, in some industrial categories (C17, C14, C24, C29), the unit value was changed by a small number of companies with large-scale water use or unit value of sampling had a large deviation. It was considered necessary to survey them periodically. The unit of industrial water usage derived by the survey in this study reflects the current industrial trends in 2016. Water use in manufacturing companies has continuously changed by the development of manufacturing technologies and simplification of manufacturing processes. In order to deal with this, it is considered necessary to survey the usage of industrial water periodically from a long-term perspective.

• International Journal of Industrial Entomology and Biomaterials
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• 제34권1호
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• pp.6-10
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• 2017

Dissolution of Antheraea yamamai silkworm cocoon was carried out in various solvent systems with various dissolving conditions including dissolution salts, salt concentration, dissolving temperature, and time. General chaotropic salt for Bombyx mori silk fibroin does not work for A. yamamai silkworm cocoon. Lithium bromide 9.3 M at $100^{\circ}C$ also does not work to dissolve wild silkworm cocoon. However, 9 M of lithium thiocyanate treatment at $100^{\circ}C$ induced 100% dissolution of wild silkworm cocoon. But it could not be dissolved lower than $60^{\circ}C$. Like lithium thiocyanate, less than $60^{\circ}C$ treatment with molten calcium nitrate 4 hydrate could not dissolve wild silkworm cocoon. As the dissolution temperature increased up to $100^{\circ}C$, the solubility of wild one was reached over 90%. SDS-PAGE showed broad tailing stream pattern that means the molecule of wild silk was depolymerized with dissolution temperature and time. From the above results, the best chaotropic salt for A.yamamai silkworm cocoon is calcium nitrate 4 hydrate.

• 한국산림과학회지
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• 제108권3호
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• pp.322-328
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• 2019

본 연구는 임도 유지관리 및 보수를 위한 기초자료를 제공하기 위하여 경상북도 영양군 임도를 대상으로 동평판재하시험(light drop weight test), 들밀도 시험 및 입도분석을 이용하여 개설시기 및 횡단위치별(성토부 차륜부(T0), 노면 중앙부(C), 절토부 차륜부(T1))에 따른 임도노반 지지력 분석을 실시하였다. 들밀도 및 입도분석결과, 들밀도는 2011년 개설임도가 $2.0694g/cm^3$으로 가장 높은 값을 나타냈으며, 2017년 개설임도가 $1.7443g/cm^3$으로 가장 낮은 값을 나타내었다. 입도분석은 2017년 개설임도에서 큰 골재의 비율이 가장 높게 나타났으며, 시기가 늦어질수록 큰 골재의 비율이 줄어드는 경향을 나타냈다. 동평판재하시험결과, 2011년 개설임도 $E_{vd}$가 평균 $35.9MN/m^2$, 2014년은 평균 $31.1MN/m^2$, 2016년은 평균 $26.7MN/m^2$, 2017년은 평균 $23.7MN/m^2$로 2011년 개설임도가 가장 높은 $E_{vd}$를 나타냈다. 또한 성토 차륜부는 평균 $21.2MN/m^2$, 절토 차륜부는 평균 $36.71MN/m^2$ 그리고 중앙부는 평균 $29.8MN/m^2$로 절토 차륜부가 가장 높은 $E_{vd}$를 나타냈다.

• Nuclear Engineering and Technology
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• 제49권7호
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• pp.1563-1570
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• 2017

In order to perform dry interim storage and transportation of the spent-fuel assemblies of the Bushehr Nuclear Power Plant, dual-purpose casks can be utilized. The effectiveness of different neutron-shield materials for the dual-purpose cask was analyzed through a set of calculations carried out using the Monte Carlo N-Particle (MCNP) code. The dose rate for the dual-purpose cask utilizing the recently developed materials of $epoxy/clay/B_4C$ and $epoxy/clay/B_4C/carbon$ fiber was less than the allowable radiation level of 2 mSv/h at any point and 0.1 mSv/h at 2 m from the external surface of the cask. By utilization of $epoxy/clay/B_4C$ instead of an ethylene glycol/water mixture, the dose rates on the side surface of the cask due to neutron sources and consequent secondary gamma rays will be reduced by 17.5% and 10%, respectively. The overall dose rate in this case will be reduced by 11%.

• Journal of applied mathematics & informatics
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• 제35권1_2호
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• pp.121-130
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• 2017

This study is concerned with the existence of positive solution for the following nonlinear elliptic system $$\{-M_1(\int_{\Omega}{\mid}x{\mid}^{-ap}{\mid}{\nabla}u{\mid}^pdx)div({\mid}x{\mid}^{-ap}{\mid}{\nabla}u{\mid}^{p-2}{\nabla}u)\\{\hfill{120}}={\mid}x{\mid}^{-(a+1)p+c_1}\({\alpha}_1A_1(x)f(v)+{\beta}_1B_1(x)h(u)\),\;x{\in}{\Omega},\\-M_2(\int_{\Omega}{\mid}x{\mid}^{-bq}{\mid}{\nabla}v{\mid}^qdx)div({\mid}x{\mid}^{-bq}{\mid}{\nabla}v{\mid}^{q-2}{\nabla}v)\\{\hfill{120}}={\mid}x{\mid}^{-(b+1)q+c_2}\({\alpha}_2A_2(x)g(u)+{\beta}_2B_2(x)k(v)\),\;x{\in}{\Omega},\\{u=v=0,\;x{\in}{\partial}{\Omega},$$ where ${\Omega}$ is a bounded smooth domain of ${\mathbb{R}}^N$ with $0{\in}{\Omega}$, 1 < p, q < N, $0{\leq}a$ < $\frac{N-p}{p}$, $0{\leq}b$ < $\frac{N-q}{q}$ and ${\alpha}_i,{\beta}_i,c_i$ are positive parameters. Here $M_i,A_i,B_i,f,g,h,k$ are continuous functions and we discuss the existence of positive solution when they satisfy certain additional conditions. Our approach is based on the sub and super solutions method.

• 대한화학회지
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• 제61권1호
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• pp.25-28
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• 2017

Solvolyses with the reaction center being the sulfur of 4-(chlorosulfonyl)biphenyl ($C_6H_5C_6H_4SO_2Cl$, 1) was studied under solvolytic conditions and the extended Grunwald-Winstein equation was applied. The thirty five kinds of solvents gave a reasonable extended Grunwald-Winstein plot with a correlation coefficient (R) of 0.940. The sensitivity values (l = 0.60 and m = 0.47) of 1 were smaller than those obtained for benzenesulfonyl chloride ($C_6H_5SO_2Cl$, 2; l = 1.10 and m = 0.61) proposed to undergo dissociative $S_N2$ mechanism. These l and m values for the solvolyses of 1 can be considered to support a $S_N2$ pathway with some ionization reaction. The activation parameters, ${\Delta}H^{\neq}$ and ${\Delta}S^{\neq}$, were determined and they are also in line with values expected for a bimolecular reaction. The kinetic solvent isotope effect (KSIE) of 1.26 is also in accord with a bimolecular mechanism, probably assisted by general-base catalysis.

• 한국식생활문화학회지
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• 제32권6호
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• pp.583-588
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• 2017

This study examined the antioxidant and neuronal cell protective effects of the water and methanol extracts of Eugenia caryophyllata Thunb. The total polyphenol content was significantly higher in the methanol extract than in the water extract. The DPPH radical scavenging activity in the water extract was similar to Vit. C at a concentration of $100{\sim}200{\mu}g/mL$. The ABTS radical scavenging activity in the water and methanol extract was similar to Vit. C at a concentration of $800{\sim}1,000{\mu}g/mL$. The superoxide dismutase (SOD)-like activity in the methanol extract was similar to Vit. C at a concentration of $800{\sim}1,000{\mu}g/mL$. The DPPH, ABTS radical scavenging and (SOD)-like activity increased with increasing extract concentration. In a cell viability using MTT, the water extract (50 and 100 ppm) and methanol extract (100 ppm) had a protective effect against $H_2O_2$-induced neurotoxicity.The result ssuggest that the extract of E. caryophyllata Thunb. has antioxidant activities and may be useful for treating neurodegenerative disorders.

• Toxicological Research
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• 제33권4호
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• pp.315-323
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• 2017

Preconceptual sex selection is still a highly debatable process whereby X- and Y-chromosome-bearing spermatozoa are isolated prior to fertilization of the oocyte. Although various separation techniques are available, none can guarantee 100% accuracy. The aim of this study was to separate X- and Y-chromosome-bearing spermatozoa using methods based on the viability difference between the X- and Y-chromosome-bearing spermatozoa. A total of 18 experimental semen samples were used, written consent was obtained from all donors and results were analysed in a blinded fashion. Spermatozoa were exposed to different pH values (5.5, 6.5, 7.5, 8.5, and 9.5), increased temperatures ($37^{\circ}C$, $41^{\circ}C$, and $45^{\circ}C$) and ROS level ($50{\mu}M$, $750{\mu}M$, and $1,000{\mu}M$). The live and dead cell separation was done through a modified swim-up technique. Changes in the sex-chromosome ratio of samples were established by double-label fluorescent in situ hybridization (FISH) before and after processing. The results indicated successful enrichment of X-chromosome-bearing spermatozoa upon incubation in acidic media, increased temperatures, and elevated $H_2O_2$. This study demonstrated the potential role for exploring the physiological differences between X-and Y-chromosome-bearing spermatozoa in the development of preconceptual gender selection.