• The Korean Journal of Physiology and Pharmacology
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• 제3권3호
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• pp.293-303
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• 1999

The influences of specific protein phosphatase and protein kinase inhibitors on the ATP-sensitive $K^+\;(K_{ATP})$ channel-opening effect of pinacidil were investigated in single rat ventricular myocytes using patch clamp technique. In cell-attached patches, pinacidil $(100\;{\mu}M)$ induced the opening of the $K_{ATP}$ channel, which was blocked by the pretreatment with H-7 $(100\;{\mu}M)$ whereas enhanced by the pretreatment with genistein $(30\;{\mu}M)$ or tyrphostin A23 $(10\;{\mu}M)$. In inside-out patches, pinacidil $(10\;{\mu}M)$ activated the $K_{ATP}$ channels in the presence of ATP (0.3 mM) or AMP-PNP (0.3 mM) and in a partial rundown state. The effect of pinacidil $(10\;{\mu}M)$ was not affected by the pretreatment with protein tyrosine phosphatase 1B $(PTP1B,\;10\;{\mu}g\;ml^{-1}),$ but blocked by the pretreatment of protein phosphatase 2A $(PP2A,\;1\;U\;ml^{-1})$. In addition, pinacidil $(10\;{\mu}M)$ could not induce the opening of the reactivated $K_{ATP}$ channels in the presence of H-7 $(100\;{\mu}M)$ but enhanced it in the presence of ATP (1 mM) and genistein $(30\;{\mu}M).$ These results indicate that the $K_{ATP}$ channel-opening effect of pinacidil is not mediated via phosphorylation of $K_{ATP}$ channel protein or associated protein, although it still requires the phosphorylation of serine/threonine residues as a prerequisite condition.

• 한국치위생학회지
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• 제11권4호
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• pp.595-602
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• 2011

Objectives : This study purpose were the effect of toothbrushing for decreasing halitosis for gingivitis and periodontitis patients. Methods : The university staffs were examined oral condition and analysised a change of oral health state score after using 3 types of toothbrushing. Results : The results were as followed : OHI-S shows that the decreasing effect takes place in the M. bass method and Tooth pick method, but shows no differences by each method. GI for Self test method shows decreasing effect after 2nd week during education while the M. bass method and Tooth pick method shows after 1st week during education. The M. bass method shows much greater effect of halitosis amongst 3 kind of method. PI for Self test method and Tooth pick method show decreasing effect. Decreasing effect during education shows until 2nd week by 3 kind of method, but it shows no differences after 3rd week. VSC(ppb) for M. bass method and Tooth pick method show decreasing effect. Decreasing effect during education shows after 3rd week by 3 kind of method, but it shows no differences until 2nd week. PHP for Self test method, M. bass method and Tooth pick method show decreasing effect after 2nd week during education. But, there is no differences of decreasing effect by among 3 kind of method. Conclusions : This study reports that it is necessary to carry outt further studies on the improvement of oral health management of adults based on the development of oral health education.

• 한국정보통신학회논문지
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• 제26권5호
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• pp.682-687
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• 2022

터널링 전계효과 트랜지스터(tunneling field-effect transistor; TFET)로 적층된 3차원 적층형 집적회로(monolithic 3D integrated-circuit; M3DIC)에 대한 연구 결과를 소개한다. TFET는 MOSFET(metal-oxide-semiconductor field-effect transistor)와 달리 소스와 드레인이 비대칭 구조이므로 대칭구조인 MOSFET의 레이아웃과 다르게 설계된다. 비대칭 구조로 인해서 다양한 인버터 구조 및 레이아웃이 가능하고, 그 중에서 최소 금속선 레이어를 가지는 단순한 인버터 구조를 제안한다. 비대칭 구조의 TFET를 순차적으로 적층한 논리 게이트인 NAND 게이트, NOR 게이트 등의 M3DIC의 구조와 레이아웃을 제안된 인버터 구조를 바탕으로 제안한다. 소자와 회로 시뮬레이터를 이용해서 제안된 M3D 논리게이트의 전압전달특성 결과를 조사하고 각 논리 게이트의 동작을 검증한다. M3D 논리 게이트 별 셀 면적은 2차원 평면의 논리게이트에 비해서 약 50% 감소된다.

• 한국정밀공학회지
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• 제4권2호
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• pp.47-55
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• 1987

Recently, in precision working, precision is in submicron. Therefore, when we measure various finished goods in superfine measurement, because it is relatively difficult to disregard effect of surroundings, these effect of surroundings must be compensated or canceled. In this study, for roundness measurement, three point method is researched which is able to cancel the effect of rotation accuracy of axis and eccenricity of workpiece. It is difference between this three point method and tradi- tional three point method whose measuring apparatus have three movable pickups posit- ioned with angle and between the pickups. As a results, when rotation accuracy of axis is varied from $0.02\mu\textrm{m}$ to $0.05\mu\textrm{m}$ the width of variation of measured roundness is $0.04\mu\textrm{m}$. And, when eccentricity of workpiece is varied from 0 to $4\mu\textrm{m}$, the width of variation of measured roundness is $0.005\mu\textrm{m}$. These error width are disregardable because they are in 10% of measured roundness. Therefore, by this three point method, the effect of rotation accuracy of axis and the effect of eccentricity of workpiece are canceled. And we are able to select the angle between the pickups ($\phi$ and $\tau$) by means of relation between $F_{k}$ and K.

• Toxicological Research
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• 제33권2호
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• pp.149-156
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• 2017

The purpose of this study was to investigate the wound healing effect of acai berry water extracts (ABWE) and a possible underlying mechanism involved in its action using various in vitro and in vivo models. The wound healing effect of ABWE was evaluated by migration assay using HS68 fibroblast cells. In addition, its effect on mRNA expression of procollagen, fibronectin, and MMP-1 was determined. Moreover, the wound healing effect of ABWE was evaluated in in vivo wound models through macroscopic and microscopic observation. In addition, mRNA expression levels of wound related genes were determined. Results revealed that ABWE was not cytotoxic. It increased migration of HS68 fibroblast cells. ABWE increased mRNA expression levels of fibronectin but decreased the mRNA expression levels of MMP-1. ABWE also showed significantly potent wound healing effect in vivo based on macroscopic and histopathological observation and mRNA expression evaluation for wound related genes. Taken together, our results indicated that ABWE might have potential as a wound healing agent.

• International Journal of Oral Biology
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• 제33권4호
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• pp.187-195
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• 2008

Reparative dentine formation requires newly differentiated odontoblast-like cells. Therefore, identification of the molecule that stimulates the odontogenic differentiation of precursor cells in the tooth pulp will be helpful for the development of strategies to repair damaged pulp. In this study, we examined the effect of N-acetylcysteine (NAC) on the odontogenic differentiation of MDPC-23 cells, a mouse odontoblast-like cell line derived from dental papilla, and primary cultured rat dental papilla cells (RDPCs). NAC (1-30 mM) suppressed production of reactive oxygen species in MDPC-23 cells in a dose-dependent manner. Although 5 to 20 mM NAC did not alter MDPC-23 cell proliferation, 1 or 30 mM NAC significantly inhibited it. NAC enhanced mineralized nodule formation and the expression of several odontoblast differentiation-associated genes in both RDPCs and MDPC-23. This NAC stimulatory effect was significant, even at concentrations lower than 1 mM. However, NAC did not stimulate expression of bone morphogenetic protein-2, -4, or -7, which are known to enhance odontogenic differentiation. Since reactive oxygen species are also involved in the pulp toxicity of resin-based restorative materials, these results suggest that NAC may be a promising candidate for supplementation of dental restorative materials in order to enhance reparative dentine formation.

• The Journal of Asian Finance, Economics and Business
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• 제7권12호
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• pp.481-490
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• 2020

Batik industry is a business activity oriented towards customer loyalty and is demanded to show an increasing diversity of products with various motives designed to prevent customers from moving to other companies. Therefore, the purpose of this study is to develop a theoretical approach in solving problems in the relationship between service quality and product diversity on satisfaction and word of mouth (WoM) and its impact on customer loyalty. Furthermore, this study also examined the mediating effects of satisfaction and WoM in the relationship between service quality and product diversity on customer loyalty. This research's object took the customers of Batik Semarangan Craftwork, Indonesia, with the phenomenon of always developing creative and innovative Batik motif designs that can be accepted by the market without leaving Semarang's uniqueness. The total sample was 98 customers. The results of the study showed that service quality and product diversity positively affected satisfaction and WoM and had a positive effect on customer loyalty. Besides, satisfaction and WoM had a mediating effect on the effect of service quality and product diversity on loyalty. Thus, it is essential for management to create and increase customer loyalty by paying attention to service quality, product diversity, satisfaction, and WoM.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2003년도 Proceedings of ACRS 2003 ISRS
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• pp.138-140
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• 2003

The aim of this study is to evaluate the effect of spatial resolutions on the accuracy to landslide susceptibility mapping. For this, landslide locations were identified in the Boun, Korea from interpretation of aerial photographs and field surveys. The topographic, soil, forest, geologic, linearment and land use data were collected, processed and constructed into a spatial database using GIS and remote sensing data. The 15 factors that influence landslide occurrence were extracted and calculated from the spatial database with 5m, 10m, 30m, 100m and 200m spatial resolutions. Landslide hazardous area were analysed and mapped using the landslide-occurrence factors by probability model, likelihood ratio, for the five cases spatial resolutions. The results of the analysis were verified using the landslide location data. In the cases of spatial resolution 5m, 10m and 30m, the verification results was similar, but in the cases of 100m and 200m the results worse than the others. Because the scale of input data was 1:5,000 ? 1:50,000, so the cases of 5m, 10m and 30m have similar accuracy but the cases of 100m and 200m have the lower accuracy. From this, there is an effect of spatial resolutions on accuracy and landslide susceptibility mapping the result is dependent on input map.

• 대한본초학회지
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• 제32권3호
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• pp.55-61
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• 2017

Objectives : The aim of this research was to determine the diverse effects of Lithospermi Radix Water Extract (LR) on human keratinocyte HaCaT cells, and to examine whether those effects could be applied to the human skin. Methods : We examined effect of LR on the cell viability of using the MTS assay in human keratinocyte HaCaT cells. The antioxidation effect of LR was analyzed relative to the well-known antioxidant resveratrol, using an ABTS assay. Quantitative RT-PCR analysis revealed that, in HaCaT cells, LR influenced the mRNA expression of tight-junction genes associated with skin moisturization. Furthermore, a wound-healing assay demonstrated altered cell migration in LR-treated HaCaT cells. Result : The cytotoxicity was confirmed to be higher in LR at a concentration of $800{\mu}g/m{\ell}$ using the MTS assay in HaCaT cells. In comparison to $100{\mu}M$ resveratrol, $1,600{\mu}g/m{\ell}$ LR showed either a similar or superior antioxidation effect. LR treatment in HaCaT cells reduced the mRNA expression levels of claudin 3, claudin 4, claudin 6, claudin 8, and ZO-2 to less than 0.80-fold, whereas JAM-A and Tricellulin mRNA expression level increased more than 1.33-fold. In addition, HaCaT cells migration was decreased to 83.9% by LR treatment. Conclusions : LR of antioxidation activity will have an anti-aging effect on the skin by reducing oxidative stress. Further studies are required to address the implications for human skin, given LR's effects of altering mRNA expression of tight junction-related gene and decreasing cell migration of HaCaT cells.

• 수산해양교육연구
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• 제24권3호
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• pp.368-377
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• 2012

In this study, a survey was held of m-learning educators in order to find out about the influence given to motivation by the characteristics of m-learning based on the parameters of the learning outcome. As a result of the analysis, it was revealed that the characteristics of m-learning provide a significant effect on the learning outcome and it was shown that the learning outcome provides a significant effect on the learning flow as well. This study aims to provide direction and guidelines for support service for learners for the improvement of the quality of m-learning to bring about enhancement in results and the fulfillment of successful learning by students.