• Proceedings of the Plant Resources Society of Korea Conference
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• 1996.09a
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• pp.70-71
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• 1996

• Proceedings of the Zoological Society Korea Conference
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• 1997.10b
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• pp.200.2-200
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• 1997

No Abstract, See Full Text

• Journal of Plant Biotechnology
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• v.29 no.4
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• pp.241-246
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• 2002

Somatic embryos or adventitious buds were formed from the segments of zygotic embryo of Lycium chinense Mill. on Murashige and Skoog (MS) medium supplemented with auxins (2,4-D, NAA, IAA) and / or cytokinins (zeatin, kinetin, BAP). Embryogenic callus formed on MS medium containing 0.5 mg/L 2,4-D and then differentiated into somatic embryos without transfer to hormone free medium. On the other hand, adventitious buds were formed on medium with 0.01 mg/L 2,4-D and 0.5 mg/L zeatin. Among various parts of zygotic embryos, the morphogenic potential was higher in the cotyledonary region, and the most organogenic potential was found in cotyledon followed by radicle, hypocotyl, and whole embryo. Histologically, bipolar structure of the heart-shaped embryos were confirmed and in adventitious buds only shoot apical meristems were found to exist.

• Korean Journal of Medicinal Crop Science
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• v.23 no.4
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• pp.319-323
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• 2015

The objective of this study was to establish the optical storage condition in cutting slips of Lycium chinense Mill. We investigated the different influential growth factor of this plant including two soil types (soil and vermiculite) and storage methods (gauze, parafilm, vinyl, and paper). Our result revealed that the formation of axillary bud was highest ($4.8{\pm}0.75ea$) from the cutting slips stored in vinyl and vermiculite treatment. Root length was long ($2.8{\pm}0.13ea$) in parafilm storage using soil. Maximum plant height was $135.33{\pm}12.81cm$ with gauze storage using vermiculite. The number of leaves was maximum ($130{\pm}2.5ea$) at 90 days from the cutting slips of gauze storage using vermiculite. Highest number of fruit was harvested ($149{\pm}16.05ea$) from the cutting slips stored in parafilm and grown in vermiculite. It can be concluded that the storage treatment and soil type influence the affecting to general growth of Lycium chinense Mill.

• Journal of Korean Society of Forest Science
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• v.95 no.5
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• pp.585-590
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• 2006

In vitro-grown axillary shot-tip meristems of Lycium chinense Mill. from cold-acclimated plant were successfully cryopreserved using a vitrification technique. After loading for 15 minutes with a mixture of 2.0 M glycerol and 0.4 M sucrose ($20^{\circ}C$), small segments (1-2 mm, 3-4 mm, and 5-6 mm) were cut from axilary buds and exposed to the cryoprotectant solution containing 30% glycerol, 15% ethylen glycol,15% dimethyl sulfoxide (DMSO), and 0.4 M sucrose at $0^{\circ}C$ for 30-120 minutes prior to direct plunge into liquid nitrogen (LN). After rapid thawing ($40^{\circ}C$), the segments were washed with MS medium containing 1.2 M sucrose for 0-35 minutes, and then transferred onto recovery-growth medium. The highest survival rate (about 90%) was obtained with cold-hardening treatment, and cryopreserved explants were successfully recovered to plantlets. No abnormal morphological changes were observed with the recovered plants after cryopreservation.

• 한국약용작물학회:학술대회논문집
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• 2008.11a
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• pp.392-393
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• 2008

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2003.10a
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• pp.165-165
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• 2003

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2003.10a
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• pp.168-168
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• 2003

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2001.02a
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• pp.292-309
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• 2001

Modulus of elasticity, modulus of rigidity, damping ratio, and natural frequency of three varieties of boxthorn(Lycium chinense Mill) (Cheongyang #2, Cheongyang gugija, and Cheongyang native) branches were analyzed. Modulus of elasticity and modulus of rigidity of the boxthorn branch was determined using standard formula after simple beam bending and torsion test, respectively, using an universal testing machine. Damping ratio and natural frequency of branches were determined using a system consisted of an accelerometer, a PC equipped with A/D converter, and a software for data analysis. Relationship between the elastic modulus and branch diameter in overall varieties and branch types showed a good correlation (r$\cong$-0.81). There was, however, no correlation between torsional rigidity and branch diameter. The internal damping results were highly variable and the overall range of the damping ratio of the boxthorn branch was 0.014 -0.087, which indicated that the branch was a lightly damped structure. The natural frequency of the boxthorn branch was in the range of 89-363 rad/s for the overall varieties and branch types. A good correlation (r$\cong$0.82) existed between the natural frequency and branch diameter in overall varieties and branch type.

• Korean Journal of Medicinal Crop Science
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• v.17 no.6
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• pp.445-451
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• 2009

This study was undertaken to develop a technique of discrimination using SSR makers in boxthorn cultivars. Forty one boxthorn cultivars, which were collected from Korea and China, were evaluated by 10 SSR markers. Total of 61 alleles were detected, ranging from 3 to 13 with an average of 6.1 alleles per locus. The averages of gene diversity and PIC values were 0.482 and 0.428, with a range from 0.25 (GB-LCM-022 and GB-LCM-087) to 0.83 (GB-LCM-167) and from 0.24 (GB-LCM-022 and GB-LCM-087) to 0.81 (GB-LCM-167), respectively. Five markers out of 10 markers, GB-LCM-022, GB-LCM-075, GB-LCM-104, GB-LCM-167 and GB-LCM-217, were selected as key markers for discrimination in boxthorn cultivars. All of boxthorn cultivars were individually distinguished by the combination of five SSR markers.