• Title/Summary/Keyword: LwA

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A study on The Design of Embedded Network Module for Web-based Remote Verification and proofreading (웹기반 원격 검.교정 시스템을 위한 임베디드 네트워크 모듈 설계에 대한 연구)

  • Kim, Min-Geun;Lee, Sang-Hun;Lee, Hyuek-Jae
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.11 no.6
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    • pp.1077-1082
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    • 2007
  • The necessity of the high speed exclusive use server that connect digital measure instrument flag to internet and CPU use rate of measure flag uses other imbedded operating system with many general servers to verification & proofreading system in remotion. This research is objective that embody high speed network module for Site-Based remote verification & proofreading system and through internet digital measure instrument flag to remote verification & proofreading, TCP/IP Offload Engine processing and improved that is Imbedded TCP/IP stack for high speed networking.

Flavobacterium jocheonensis sp. nov., Isolated from Marine Green Alga Ulva pertusa

  • Choi, Ha Ri;Park, So Hyun;Heo, Moon Soo
    • Journal of Microbiology and Biotechnology
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    • v.29 no.8
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    • pp.1266-1272
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    • 2019
  • A bacterial strain, labeled $UR11^T$, was isolated from green alga Ulva pertusa collected from Jeju Island, Korea. $UR11^T$ was identified as a gram-negative, rod-shaped, motile by gliding and aerobic bacterial strain with yellow colonies on R2A plates. The strain $UR11^T$ grew over at a temperature range of $10^{\circ}C$ to $30^{\circ}C$ (optimally at $25^{\circ}C$), a pH range of 6.0-11 (optimally at pH 7.0) and a Nacl range of 0.5-5% Nacl (w/v). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain $UR11^T$ was a member of the genus Flavobacterium. Strain $UR11^T$ shared close similarity with F. jejuensis $EC11^T$ (98.0%) F. jumunjinense $HME7102^T$ (96.1%), F. haoranii $LQY-7^T$ (95.3%), F. dongtanense $LW30^T$ (95.1%), and F. ahnfeltiae 10Alg $130^T$(94.9%). The major fatty acids (>5%) were $iso-C_{15:0}$ (33.9%), $iso-C_{15:1}$ G (12.4%), $iso-C_{17:0}$ 3-OH (9.0%), $isoC_{16:0}$ (7.0%) and $iso-C_{15:0}$ 3-OH (6.3%). The major polar lipids were phosphatidylethanolamine, seven unknown aminolipids, two unknown aminopolarlipids and two unknown lipids. DNA-DNA hybridization value was 58% at strain $UR11^T$ with F. jejuensis $EC11^T$. Based on phenotypic, chemotaxonomic and phylogenetic evidence, strain $UR11^T$ represents a novel species of the genus Flavobacterium, for which the name Flavobacterium jocheonensis sp. nov. is proposed. The type strain is Flavobacterium jocheonensis is $UR11^T$ (=KCTC $52377^T$ =JCM $31512^T$).

Isolation and identification of Vibrio harveyi from chub mackerel (Scomber japonicus)

  • Lee, Young-Ran;Jun, Jin-Woo;Giri, Sib Sankar;Kim, Hyoun-Joong;Yun, Sae-Kil;Chi, Cheng;Kim, Sang-Guen;Koh, Jeong-Rack;Jung, Ji-Yun;Park, Se Chang
    • Korean Journal of Veterinary Research
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    • v.56 no.1
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    • pp.45-46
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    • 2016
  • For several days, there was a series of mortalities of chub mackerel (Scomber japonicus) that were reared for public exhibition in a private aquarium in Seoul, Korea. As part of the diagnosis of the dead fish, a bacterial isolate from the kidney was cultured, identified, and confirmed to be Vibrio (V.) harveyi using Vitek System 2 and 16S rRNA gene sequencing. Phylogenetic analysis was also performed by the neighbor-joining method. As a result, the V. harveyi isolated from chub mackerels of a private aquarium in Korea, called as SNUVh-LW1, was clustered in the same group with V. harveyi ATCC33843.

Feed Intake, Nutrient Utilization and Growth Rate of Jamunapari Goats Fed Sundried Leucaena leucocephala

  • Srivastava, S.N.L.;Sharma, K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.11 no.4
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    • pp.337-343
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    • 1998
  • In a feeding trial, Jamunapari male kids (18) of about 4 months age were equally divided into two groups of nine animals each. Goats in the experimental group were fed sun-dried pelleted Leucaena leucocephala leaves and those in the control group were offered a conventional diet without Leucaena leaves as per Kearl (1982) recommendations for a period of 6 months. Daily dry matter intake DMI/100 kg BW was $3.13{\pm}0.04kg$ in the Leucaena group and $3.30{\pm}0.05kg$ in the control. There were significant (p < 0.01) differences in the apparent digestibilities of DM, OM, CP, EE, CF and NFE being lower in the Leucaena group. Contents of digestible crude protein (DCP) and total digestible nutrients (TDN) were 11.40 and 52.20%, respectively, in the Leucaena group and 14.04 and 66.10%, respectively in the control. The nitrogen in the Leucaena group was not well utilized as compared to the control, though kids were in positive nitrogen balance in both the groups. The average daily weight gain of kids on pelleted Leucaena was $29.95{\pm}2.60g$ as against $42.09{\pm}3.24g$ observed in the control. The mean DMI/kg LW gain was significantly (p < 0.01) higher in the Leucaena group ($14.70{\pm}0.78kg$) as compared to the control ($11.55{\pm}0.46kg$). The Hb, BUN, SGOT and SGPT concentrations were statistically similar in both the groups. Histopathological examination of thyroid gland of goats sacrificed at the end of experiment did not reveal any signs of colloidal goitre associated with mimosine toxicity. No significant pathological alterations were observed in vital organs irrespective of dietary treatment. Sundried, pelleted Leucaena foliage appears to be a promising potential feed for growing goats without any significant deleterious effect.

Oviduct-specific Glycoprotein 1 Locus is Associated with Litter Size and Weight of Ovaries in Pigs

  • Niu, B.Y.;Xiong, Y.Z.;Li, F.E.;Jiang, S.W.;Deng, C.Y.;Ding, S.H.;Guo, W.H.;Lei, M.G.;Zheng, R.;Zuo, B.;Xu, D.Q.;Li, J.L.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.5
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    • pp.632-637
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    • 2006
  • Oviduct-specific glycoprotein 1 (OVGP1) is implicated in playing a role in fertilization and early embryo development. In this study, we have obtained the sequence of intron 9 of OVGP1 gene in swine. Comparative sequencing of Meishan (a native Chinese breed) and Large White pig breeds revealed an A/T substitution at position 943. A PCR-EcoRI-RFLP assay was developed to detect this mutation. Polymorphism analysis in Qingping animals showed that pigs with BB genotype had lower number of piglets born alive (NBA) in multiple parities than pigs with AA (p<0.05) and AB genotype (p<0.01). In Large $White{\times}Meishan$ ($LW{\times}M$) $F_2$ offspring, the weight of both ovaries (OW) of the BB genotype was significantly lighter than that of AB (p = 0.05) and AA (p<0.01) genotypes. Analysis of the data also revealed that the mutation locus affected these two traits mostly by additive effects. These studies indicated that the polymorphism was associated with NBA and OW in two distinct populations and further investigations in more purebreds or crossbreds are needed to confirm these results.

Effects of Perinatal Nutrition on Metabolic and Hormonal Profiles of Goat Kids (Capra hircus) during Their First Day of Life

  • Celi, Pietro;Di Trana, Adriana;Claps, Salvatore;Di Gregorio, Paola
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.11
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    • pp.1585-1591
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    • 2008
  • The aim of the present work was to monitor metabolic and hormonal profiles in newborn kids, born from dams fed diets with low or high levels of energy requirements. Starting from the last month of pregnancy, 14 goats were randomly allocated to two groups: Group LD (low diet) and Group HD (high diet) that received a diet that covered 80% and 140% of their energy requirement, respectively. At delivery, the kids were weighed and a blood sample was taken before they suckled colostrum (Time 0) and 1, 2, 3, 12 and 24 h after they started suckling. Plasma insulin, IGF-I, glucose, fT3 and fT4 concentrations were not influenced by the dietary treatments, but a significant effect of time was observed as they progressively increased during the first 12 h of life. Plasma cholesterol, triglyceride, albumin, globulin and total protein plasma concentrations were significantly higher in Group HD than those of Group LD. In Group HD, cortisol concentrations were significantly lower than those of Group LD. Positive correlations were observed between LW and IGF-I (r = 0.71; p<0.05), plasma insulin and glucose (r = 0.79; p<0.05) and total protein and globulin concentrations (r = 0.97; p<0.001). Our results show that perinatal nutrition affects newborn goat kids' metabolic and hormonal profile.

Studies on the production and purification of an extracellular protease from a nonpigmenting Serration sp. (Nonpigmenting Serratia sp.에서 균체의 단백질 분해효소의 생성과 정제에 관한 연구)

  • Kim, Soung-Soo
    • Microbiology and Biotechnology Letters
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    • v.13 no.4
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    • pp.321-327
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    • 1985
  • Cultivation conditions for the production of extracellular alkaline protease by a nonpiamentation Serratia sp. and purification of the enzyme were studied. The maximum enzyme level was obtained at the beginning of stationary phase when the organism was cultured on brain heart infusion medium at $25^{\circ}C$ under aeration (gyratory shaking, 180 cycles/min). The enzyme was purified about 100 fold with 16.5% yield by ammonium sulfate precipitation, ammonium sulfate fractionation followed by DEAE-cellulose chromatography (1st and 2nd). The purified enzyme moved as a single symmetrical peak in the analytical ultracentrifuge. The enzyme demonstrated its maximum activity at pH 8.5-9.0 and 4$0^{\circ}C$ when vitamin-free casein was used as a substrate.

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Disabled Alpine Ski Athlete's Kinematic Characteristic Changes by Computer Aided Design Based Mono Ski Bucket: A Case Study (컴퓨터 디자인 기반 모노스키 버킷 사용에 따른 장애인 알파인 스키 선수의 운동학적 특성 변화 연구: 사례 연구)

  • Koo, Dohoon;Eun, Seondeok;Hyun, Boram;Kweon, Hyosun
    • Korean Journal of Applied Biomechanics
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    • v.24 no.4
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    • pp.425-433
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    • 2014
  • The purpose of the study was to investigate the effect of CAD (Computer Aided Design) based alpine mono-ski bucket design on disabled ski athletes' kinematic characteristics. Two national team ski athletes with LW11 disabilities (Locomotion Winter Classification) category for sit ski participated in both pre and post experiment. Both of the subjects performed 3 trials of carved turn on a ski slope under two conditions. Where, subject "A" performed pre experiment with personal bucket and post experiment with the newly developed CAD based bucket whereas, Subject "B" as control subject performed both pre and post experiment with his personal bucket. For the experiment, 24 Infrared cameras were positioned on the ski slope which covered the path of the ski turn. Also, motion capture suit with reflective markers were worn by both subjects. In the result, decrement in medial/lateral displacement of COM, anterior/posterior displacement of COM, flexion/extension angle of trunk as well as velocity losing rate of COM was observed in subject "A" when using the newly developed CAD based bucket. In contrast, no larger effect on performance was observed when using personal buckets. In conclusion, the findings obtained from the study indicated effectiveness of newly developed CAD based bucket by reducing excessive movement of hip and trunk which is an important factor to perform an effective turn.

Excavation Support Design and Stability Analysis of Shallow Tunnel in Heavily Fractured Rock Mass (연약 파쇄 지반내 터널의 굴착.보강 설계 및 안정성 분석)

  • Shin, Hee-Soon;Synn, Joong-Ho;Park, Chan;Han, Kong-Chang;Choi, Young-Hak;Choi, Yong-Ki
    • Proceedings of the Korean Geotechical Society Conference
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    • 2000.03b
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    • pp.87-92
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    • 2000
  • In excavation of tunnels especially located in shallow depth, it is not rare to meet geological change in excavation progress worse than expected in the initial design stage. This paper present a case study on the re-design of excavation and support system of a shallow tunnel under construction where it meets the unexpected bad geological condition during excavation. The detailed geological investigation shows that the rock mass is heavily weathered and fractured with RMR value less than 20. Considering this geological condition, the design concept is focused on the reinforcement of the ground preceding the excavation of tunnel. Two design patterns, LW-grouting & forepoling with pilot tunnelling method and the steel pipe reinforced grouting method, are suggested. Numerical analysis by FLAC shows that these two patterns give the tunnel and roof ground stable in excavation process while the original design causes severe failure zone around the tunnel and floor heaving. In point of the mechanical stability and the degree of construction, the steel pipe reinforced grouting technique proved to be good for the reinforcement of heavily fractured rock mass in tunnelling. This assessment and design process would be a guide in the construction of tunnels in heavily weathered and fractured rock mass situation.

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Seasonal Variations in Voluntary Intake and Apparent Digestibility of Forages in Goats Grazing on Introduced Leymus chinensis Pasture

  • Sun, Zewei;Wang, Zaisen;Zhong, Qingzhen;Zhou, Daowei
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.6
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    • pp.818-824
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    • 2014
  • The nutrient composition of pasture, voluntary intake and digestibility of diet ingested by goats grazing on an introduced Leymus chinensis pasture were measured across spring (May), summer (July), autumn (October) and winter (March). In each season, 12 Inner Mongolian Cashmere goats (6 wethers and 6 does with an average live weight of $22.2{\pm}1.3$ kg and $19.5{\pm}0.8$ kg, respectively) were used to graze on a 2 hectares size paddock. Diet selection was observed and the plant parts selected by grazing goats and whole plant L. chinensis were sampled simultaneously. The alkane pair $C_{32}:C_{33}$ and $C_{36}$ were used to estimate intake and digestibility, respectively. The results showed that the plant parts selected by goats had higher crude protein (CP) and lower acid detergent fiber (ADF) and neutral detergent fiber (NDF) than the whole plant, especially in the autumn and winter. The voluntary intake of dry matter (DM), CP, ADF, NDF, and metabolizable energy (ME) by goats was highest in summer (p<0.05). The goats ingested more CP, ME, and less ADF in spring than in autumn (p<0.05). The intakes of DM, CP, and ME were lowest in winter (p<0.05). There were significant differences in nutrient intake between wethers and does in each season, except for the ADF and ME intake per metabolic weight ($LW^{0.75}$). The nutrient digestibilities were higher in spring and summer, and decreased significantly during the autumn and winter (p<0.05). Goats, especially wethers, had a relative constant NDF digestibility across seasons, however, the apparent digestibility of CP in both wethers and does, decreased to negative values in winter. The grazing goats experienced relatively sufficient nutrients supply in spring and summer, and a severe deficiency of CP and ME in winter.