• The Korean Journal of Cytopathology
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• v.7 no.2
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• pp.163-168
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• 1996

Abnormalities of p53 gene are common in lung cancers and are associated with immunologically detectable p53 protein. p53 immunoreactivity is uncommon in normal cells but is frequently seen in neoplasia. Therefore, assessment of p53 expression may assist in the cytological diagnosis of malignancy. The usefulness of p53 immunostaining as a marker of malignancy in the cytological analysis of bronchial brush specimens from the patients with lung cancers was investigated in this study. A total of 71 bronchial brush samples submitted for cytologic diagnosis were immunostained with D07, a monoclonal antibody to recombinant p53 protein. Resultant p53 data were correlated with cytologic diagnosis and clinical information. Of the 17 smears with a benign cytodiagnosis, all were p53 negative. Of the 40 cases with a malignant cytodiagnosis (histologically confirmed), 35 were p53 positive and 5 were negative. Of the 14 cases that were cytologically suspicious but nondiagnostic for malignancy, 11 were p53 positive, 9 of which were subsequently proved to be malignant by histologic examination, and the remaining 2 cases were tuberculosis clinically. Forty four of 51 histologically confirmed lung carcinomas were p53 positive, including 25 of 28 squamous cell carcinomas, 13 of 17 small cell carcinomas, 3 of 3 adenocarcinomas, and 3 of 3 large cell undifferentiated carcinomas. These results suggest that p53 immunostaining could be of value as a marker of malignancy in the cytologic examination of bronchial brush specimens. Furthermore, we have shown the possible clinical utility of p53 immunostaining in cytopathological diagnosis, that is, as a valuable adjunct to morphological assessment in the analysis of cytopathologically suspicious cases.

• Korean Journal of Clinical Laboratory Science
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• v.39 no.3
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• pp.217-222
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• 2007

In the last 5 years the Epidermal Growth Factor Receptor (EGFR) has emerged as one of the most important targets for drug development in oncology. Monoclonal antibodies targeting the external domain of EGFR have been shown to have clinical benefits in colorectal and head and neck cancer when combined with chemotherapy and/or radiation. Also the targeting of the epithelial growth factor receptor (EGFR) kinase domain using the closely related inhibitors gefitinib and erlotinib has generally been ineffective against solid tumors, many of which over express the receptor. We found that there were some differential expressions according to primary antibodies of the EGFR protein which being used as one of the histological tumor markers for non-small cell lung cancer (NSCLC). We also found that there are some differential expressions according to antibodies, the pH of the antigen retrieval (AR) buffer solutions and kinds of enzymes. There were some differential expressions according to the secondary antibodies and the detection systems. We analyzed the correlations between the immunohistochemical expressions of the EGFR protein and the gene mutations of the EGFR. The differences between automatic stainers and manual staining methods were also evaluated.

• Tuberculosis and Respiratory Diseases
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• v.72 no.2
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• pp.132-139
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• 2012

Background: Periostin is preferentially expressed in periosteum, indicating a potential role in bone formation. Recently, there have been emerging controversies about its role in invasion and metastasis of human malignancies. We attempted to determine the clinicopathological significance of periostin expression in non-small cell lung carcinoma (NSCLC). Methods: Immunohistochemical staining of periostin protein from 91 cases of NSCLCs was performed using tissue microarray blocks. The results were correlated with clinicopathological parameters. Results: Positive reaction to periostin was predominantly noted in the tumor stroma. The strongest reaction presented as a band-like pattern just around the tumor nests. Non-neoplastic lung tissue and most in-situ carcinomas did not show a positive reaction in their stroma. With respect to tumor differentiation, moderate to poor differentiated tumors (47/77) revealed even higher periostin expression than the well-differentiated ones (4/14) (p=0.024). High periostin expression was positively correlated with E-cadherin and p53 expression, but was not related with patient age, sex, tumor type, PCNA index, b-catenin, cyclin D1, pTNM-T, pTNM-N, stage, and patient survival (p>0.05). Conclusion: These results suggest that periostin might play a role during the biological progression of NSCLC, but may not be related to the clinical prognostic parameters.

• Korean Journal of Veterinary Research
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• v.27 no.2
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• pp.269-275
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• 1987

Radiation-induced life shortening, carcinogenesis and other pathological changes were investigated in NIH(GP) mice after $^{60}Co-{\gamma}$ ray irradiation(100~700rads). The results were summarized as follows: 1. There were little difference in body weights, hematological examination and other clinical findings between normal and irradiated groups. 2. Mean survival time of the mice after irradiation were decreased dose-dependently. 3. Main gross findings of the mice irradiated were appeared as enlargement of spleen, thymus and lymph nodes, tumorous nodules of lung and cyst of ovary. Especially, enlargement of thymus was promineort in high dose group. 4. Microscopically, there were various findings including myeloid leukemia, thymic lymphoma, lung adenoma, adenosquamous cell carcinoma of pancreas, pneumonia and other pathological changes. Especially thymic lymphoma was highly frequent in the 700 rads group.

• Toxicological Research
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• v.30 no.2
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• pp.65-70
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• 2014

Recent episodes of severe air pollution in eastern Asia have been reported in the scientific literature and news media. Therefore, there is growing concern about the systemic effects of air pollution on human health. Along with the other well-known harmful effects of air pollution, recently, several animal models have provided strong evidence that air pollutants can induce liver toxicity and act to accelerate liver inflammation and steatosis. This review briefly describes examples where exposure to air pollutants was involved in liver toxicity, focusing on how particulate matter (PM) or carbon black (CB) may be translocated from lung to liver and what liver diseases are closely associated with these air pollutants.

• Proceedings of the KTCVS Conference
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• 1994.04a
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• pp.10-16
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• 1994

• The Journal of the Korean life insurance medical association
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• v.9
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• pp.8-9
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• 1990

• Journal of Physiology & Pathology in Korean Medicine
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• v.27 no.1
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• pp.130-135
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• 2013

We investigated the lung function change and quality of life in smokers after taking herbal medicine. The patients were divided into two groups(smoking and non-smoking). We measured lung function change by VRI and quality of life by BDI and Fatigue Scale Score before and after herbal medicine intake. And we examined changes of nicotine dependence, total smoking amount, tobacco flavor in smoking group. The VRI energy bar could not represent the change of the lung function in smoking group and non-smoking group. And there was no change of lung function by VRI in two group patients before and after herb medication. But after taking herb medicine, the patients who have abnormal sound in VRI were improved. The herbal medicine does not affect to the amount of smoking, tobacco flavor, nicotine dependence in Smoking patients. Smoking group were higher than non-smokers in BDI score and Fatigue Scale score. Smoking group were more improved than the non-smokers in BDI and Fatigue Scale Score after taking herbal medicine.

• Journal of Sasang Constitutional Medicine
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• v.30 no.2
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• pp.1-7
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• 2018

Objective This study was designed to define the efficacy of medicinal herbs of Taeeumin in Sasang constitutional medicine. Method We searched and analyzed original text such as Donguisusebowon Sinchuk edition written in 1901, Donguisusebowon Gabo edition written in 1894, Donguisusebowon Sansang Chobongwon, and posthumous manuscripts left by Je-ma Lee published by the Ministry of Health of North Korea. Results Seven herbs such as Ephedrae Herba, Coicis Semen, Castaneae Semen, Fel Ursi, Mori Cortex Radicis, Ginkgonis Semen, and Farfarae Flos regulate the mechanism of Esophagus-Cold (Wiwanhan). Three herbs such as Melonis Pedicellus, Ailanthi Radicis Cortex, and Quisqualis Fructus regulate the mechanism of Cold Lung-Dry (HanpaeJo). Sixteen herbs such as Rhei Rhizoma, Angelicae Tenuissimae Radix, Puerariae Radix, Cimicifugae Rhizoma, Angelicae Dahuricae Radix, Moschus, Gleditsiae Spina, Mume Fructus, Aurum, Glycine Semen Germinatum, Ampelopsis Radix, Cornu rhinocerotis, Antelopis Cornu, Bomeolum, Bezoar Bovis, and Typhae Pollen regulate the mechanism of Liver-Heat (Ganyeol). Three herbs such as Chrysanthemi Indici Flos, Nelumbinis Semen, and Spirodelae Herba regulate the mechanism of Heat Lung-Dry (YeolpaeJo). Conclusion Forty four herbs of Taeeumin regulate the mechanisms of Esophagus-Cold (Wiwanhan), Cold Lung-Dry (HanpaeJo), Liver-Heat (Ganyeol), Heat Lung-Dry (YeolpaeJo) and correct the energy-fluid pathology of Taeeumin.

• Tuberculosis and Respiratory Diseases
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• v.40 no.4
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• pp.416-423
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• 1993

Pulmonary alveolar proteinosis(PAP) is thought to be a rare disease of unknown etiology characterized by the accumulation of strong PAS-positive lipoproteinaceous material in the pulmonary alveolar spaces. The defect in the clearance and degradation of intra-alveolar phospholipoproteinaceous material in PAP likely represents dysfunction of type II pneumocytes. Although the causative treatment of PAP is not well known, yet whole lung bronchopulmonary lavage is a relatively safe and effective treatment. We experienced three cases of PAP, which were confirmed by light and electron microscopic examinations of lung tissues obtained by open lung biopsy, transbronchial lung biopsy and lung lavage, so we present 3 cases of PAP with a review of the literature.