• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.116-116
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• 2017

Low-temperature is one of the environmental stress factors that affect plant growth and development and consequently limit crop productivity. The control of seed germination under low-temperature is organized by many genes which are called quantitative trait loci (QTLs). High germination rate for low-temperature is an important factor of growing rice. Previously, we identified a major QTL controlling low-temperature germinability in rice using 96 introgression lines (ILs) derived from a cross between Oryza rufipogon (Rufi) and the Korean japonica cultivar, 'Hwaseongbyeo (HS)'. A $BC_3F_7$ line (TR5) showed better low-temperature germinability than its recurrent parent. TR5 was crossed with HS to develop a segregating F2:3 populations for the target QTL. Six SSR markers polymorphic between HS and Rufi were used to screen and fine map the qLTG1. The qLTG1 on chromosome 1, which accounted for 55.5% of the total phenotypic variation, confirmed that Rufi allele enhanced the low-temperature germinability. Intervals between markers CRM16 and CRM15, four candidate genes were identified. The identified candidate genes, which are encoded by a protein of unknown function, showed their direct involvement on seed germination at low-temperature. To identify genes targeted by qLTG1, we investigated the expression profiles of these candidate genes and germination behavior of qLTG1 under different stress conditions and compared to HS, Rufi, and TR5 at $13{\pm}2^{\circ}C$ for 3 days after incubation. Furthermore, transgenic rice plants will also be developed to conduct a detailed investigation on low-temperature germinability. Hence, the QTL for low-temperature germinability would be useful in rice breeding programs especially in the development of lines possessing low-temperature germinability.

• 농업과학연구
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• 제39권4호
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• pp.477-482
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• 2012

Low-temperature germination is one of the major determinants for stable stand establishment in the rice direct seeding method in temperate regions and at high altitude areas. Quantitative trait loci (QTL) controlling low-temperature germinability in rice were identified using 96 introgression lines (ILs) derived from a cross between Oryza rufipogon and the Korean japonica cultivar, 'Hwaseongbyeo'. The germination rate at $15^{\circ}C$ was measured to represent low-temperature germination and used for QTL analysis. The germination rate at $15^{\circ}C$ for 7 days of Oryza rufipogon and Hwaseongbyeo was 93.3 and 28.7%, respectively, and that of progenies ranged from 0 to 48%. A linkage map was constructed using 135 simple sequence repeat (SSR) markers. Five putative QTLs associated with low-temperature germination were detected on chromosomes 1, 3, 4, 10 and 11. The QTL, qltg10 on chromosome 10 accounted for 19.2% of the total phenotypic variation for low-temperature germinability. Four additional QTL, accounted for 10.4 - 15.1% of the total phenotypic variation. The O. rufipogon alleles in all detected QTLs loci increased the low-temperature germination rate. No QTL associated with low temperature germinability has been detected near the qltg10 QTL in this study suggesting that qltg10 is a new QTL. The locus, qltg10 is of particular interest because of its independence from undesirable height and maturity effects. The DNA markers linked to the QTL for low temperature germinability would be useful in selecting lines with enhanced low temperature germinability in rice breeding program.

• 한국작물학회지
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• 제8권1호
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• pp.37-45
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• 1970

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.192-192
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• 2017

Direct-seeding cultivation of rice is increasing in Asia instead of transplanting system, because of its lower cost and operational simplicity. Low-temperature germinability (LTG) and anaerobic germinability (AG) are important characters for breeding of varieties for wide-spread adoption of direct-seeding cultivation in rice. This study was performed to characterize LTG and AG of seven rice varieties and identify varieties with strong germinability on both low-temperature and anaerobic conditions. The mean germination rate and germination vigor of seven varieties were 51.7% and 6.0 under low-temperature condition, respectively. Among these varieties, Cheongcheongjinmi and Hwanggeumnodeul had the highest germination rate of 80%, indicating that Cheongcheongjinmi and Hwanggeumnodeul have a good LTG. In anaerobic conditions, the germination rate and coleoptile length for all varieties were 47.6% and 3.2 cm, respectively. Of them, the highest germination rate and coleoptile length were observed in Subo and Hopum, respectively, suggesting that these two varieties are tolerant to anaerobic during germination stage. Molecular characterization by SDS-PAGE revealed that the protein patterns differed at 50 kDa, 40 kDa, and 22 kDa between low-temperature and anaerobic conditions. Varieties identified as good LTG or AG in this study may be used for developing new direct-seeding rice cultivars through pyramiding these traits in the breeding program.

• 한국작물학회지
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• 제40권6호
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• pp.711-715
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• 1995

수도 저온발아성이 양호한 품종의 육성을 위한 효율적인 저온발아성 검정 조건을 모삭하기 위해 최근 육성된 신품종과 도입품종을 합하여 50품종을 공시, 1994년 영남농업시험장에서 13 , 16 , 25 에서 20일간 발아시험을 실시한 바, 그 결과를 요약하면 다음과 같다. 1. 13 , 16 , 25 에서 발아시킨 50품종들의 발아율 기초통계량 분석 결과, 13 11일 치상일에서 평균발아율이 43.8%, 발아율의 절위가 92, 그리고 분산이 748로 가장 높아 품종간 저온발아성의 차가 가장 현저한 조건이었다. 2. 13 11일 치상일의 발아율과 평균발아일수, 발아계수와의 상관에서 고도의 상관이 검증되어 13 11일 치상일의 발아율로써 저온발아성을 나타내는 지표로 시용 가능할 것으로 여겨진다. 3. 13 11일 치상일의 발아율을 근거로 공시품종들의 저온발아성을 구분해본 바, 동진벼 등 11품종이 저온발아성이 양호하였고 상주벼 등 17품종이 저온발아성이 매우 불량하였다. 4. 공시품종의 저온발아성과 몇가지 미질관련형질들과의 상관에서 출수 소요일수 및 아밀로스함량은 고도의 정의 유의상관이, 입폭과는 부의 유의상관이 인정되었다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.283-283
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• 2022

Abiotic stresses such as high/low temperature, drought, salinity, and submergence directly or indirectly influence the physiological status and molecular mechanisms of rice which badly affect yield. Especially, the low temperature causes harmful influences in the overall process of rice growth such as uneven germination and the establishment of seedlings, which has become one of the main limiting factors affecting rice production in the world. It is of great significance to find the candidate genes controlling low-temperature tolerance during seed germination and study their functions for breeding new rice cultivars with immense low-temperature tolerance during seed germination. In this study, 120 lines of Cheongcheong/Nagdong double haploid population were used for quantitative trait locus analysis of low-temperature germinability. The results showed significant difference in germination under low different temperature conditions. In total, 4 QTLs were detected on chromosome 3, 6, and 8. A total of 41 genes were identified from all the 4 QTLs, among them, 25 genes were selected by gene function annotation and further screened through quantitative real time polymerase chain reaction. Based on gene function annotation and level of expression under low-temperature, our study suggested OsGPq3 gene as a candidate gene controlling viviparous germination, ABA and GA signaling under low-temperature. This study will provide a theoretical basis for marker-assisted breeding.

• 한국작물학회지
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• 제42권6호
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• pp.766-777
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• 1997

수도 저하발아성에 대한 유전 양상을 구명하여 저온발아성 품종 육성의 기초 자료를 얻고자 숙기가 다른 세 그룹에서 10품종을 선정 '94년에 15일 간격 4시기로 파종 및 이앙을 실시하여 수량한 종자를 '95년 4~5월에 13$^{\circ}C$ 항온 실내 시험과, 건답 직파의 포장 시험으로 저온발아성을 조사하고, 저온발아성 고 1품종, 저 1품종, 중 4품종을 선정 '94 하계에 절영법에 의한 정역이면교잡을 실시하여 얻어진 F$_1$ 교배입을 '95년 5월에 13$^{\circ}C$ 항온에서 저온발아 시험을 실시하여 얻은 결과를 요약하면 다음과 같다. 1. 재배 시기를 달리하여 수량된 종자의 실내 및 포장에서의 저온발아성 분산분석 결과 품종에 따라 재배시기간에 고도의 유의차가 인정되었다. 2. 실내 13$^{\circ}C$ 항온 저온발아계수와 포장의 자연상태 건답직파에서의 출아계수와의 상관분석 결과 고도의 유의한 상관(r=0.5111)이 인정되었다. 3. 조합능력 분석 결과 일반조합능력 및 특정조합능력 모두 고도의 유의성이 인정되었으며, 일반조합능력이 특정조합능력보다 커서 상가적작용이 상위성 작용보다 더 크게 나타났다. 4. 정역차이 분산분석 결과 정역간에 고도의 유의차가 인정되었다. 5. 부친열 공분산/모친열 공분산 Graph에서 6개의 교배열 모두가 모본 효과를 보였으며, Alpha와 Beta 유전 분석 결과, 저온발아성의 정역간 차이는 Alpha 유전의 효과로 나타났다. 6. 평균우성도 분석 결과, 30개 조합 중 17개 조합에서 초월분리를 보였으며, 그 중 9개 조합이 저온발아성이 낮은 쪽으로 초월분리를 보였다.

• 한국작물학회지
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• 제39권2호
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• pp.187-192
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• 1994

본 연구는 소백벼, 중원벼 등 국내 재배품종과 교배모본으로 이용되는 재래품종을 공시하여 벼 품종의 현발아성의 차이를 알고자 수행되었으며 결과를 요약하면 아래와 같다. 1. 공시품종중 소백벼, 오대벼, 화성벼 및 자광도 등 자포니카형 품종은 출수 후 20일째부터 높은 수발아율을 보였으나 통일형인 중원벼와 인디카형인 IR-20은 출수후 40일에도 낮은 수발아율을 보였다. 2. 발아온도가 15$^{\circ}C$ 에서 3$0^{\circ}C$ 로 높아질수록 공시 품종 모두 수발아율과 초기발아세가 높아졌으며, 15$^{\circ}C$ 에서는 치상 후 4일까지 거의 수발아하지 않았다. 온도에 따른 수발아의 변화양상은 자포니카형 3품종과 샤레형 재래종인 자광도가 비슷하였고, 통일형인 중원벼는 인디카형 품종 IR-20과 유사하였다. 3. 평균온도가 동일한 경우 항온조건보다는 주야간 변온조건에서 초기의 수발아율이 다소 낮았으며, 품종간 수발아성의 차이는 포장상태의 기온을 고려하여 주야 25/15$^{\circ}C$ 의 변온 조건에서 치상 후6일째에 조사하는 것이 합리적이었다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 1996년도 춘계 학술대회지
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• pp.12-13
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• 1996

• Plant Biotechnology Reports
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• 제3권1호
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• pp.103-109
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• 2009

In this study we established reliable methods for conservation of seeds of Phaius tankervilleae as an orchid genetic resource. The seeds, which were dehydrated to 5% water content and preserved at $4^{\circ}C$, showed no decrease in viability and germinability after three months. After storage for six months, however, the seeds showed a drastic decrease in germinability, even though survival rate was high. For long-term preservation of seeds of P. tankervilleae, cryopreservation is applied to the freshly harvested seeds. When the seeds were cryopreserved by the vitrification method for up to 12 months there was no apparent deterioration effect of storage time. These results indicate that cryopreservation by the vitrification method is useful for long-term conservation of P. tankervilleae seeds, which are difficult to preserve for more than three months under dry and low-temperature conditions.