Plant Biotechnology Reports

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Cryopreservation and low-temperature storage of seeds of Phaius tankervilleae

  • Hirano, Tomonari (Laboratory of Plant Cell Technology, Faculty of Horticulture, Chiba University) ;
  • Godo, Toshinari (Botanic Gardens of Toyama) ;
  • Miyoshi, Kazumitsu (Crop Science Laboratory, Faculty of Bioresources, Akita Prefectural University) ;
  • Ishikawa, Keiko (Japan Horticultural Production and Research Institute) ;
  • Ishikawa, Masaya (Environmental Stress Research Unit, Division of Plant Sciences, National Institute of Agrobiological Sciences) ;
  • Mii, Masahiro (Laboratory of Plant Cell Technology, Faculty of Horticulture, Chiba University)
  • Received : 2008.06.05
  • Accepted : 2008.11.18
  • Published : 2009.02.28
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Abstract

In this study we established reliable methods for conservation of seeds of Phaius tankervilleae as an orchid genetic resource. The seeds, which were dehydrated to 5% water content and preserved at $4^{\circ}C$, showed no decrease in viability and germinability after three months. After storage for six months, however, the seeds showed a drastic decrease in germinability, even though survival rate was high. For long-term preservation of seeds of P. tankervilleae, cryopreservation is applied to the freshly harvested seeds. When the seeds were cryopreserved by the vitrification method for up to 12 months there was no apparent deterioration effect of storage time. These results indicate that cryopreservation by the vitrification method is useful for long-term conservation of P. tankervilleae seeds, which are difficult to preserve for more than three months under dry and low-temperature conditions.

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