• Ocean and Polar Research
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• v.37 no.1
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• pp.37-47
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• 2015

The effects of low salinity (fertilization success and larval survival) on the limpet Cellana grata were studied at early stages of development using the marine bioassay technique. It was shown that, under normal conditions for development from fertilization to the post veliger stage, the salinity must be not less than 20.0~35.0 psu. However, the fertilization rate and larval survival of C. grata was obviously reduced at 5.0 psu and 10.0 psu, respectively. Mass mortality was estimated to occur at <20.0 psu (48-h $EC_{50}=19.54psu$) and the survival rate of normal veliger larvae decreased with experimental time during exposure. No observed effective concentration (NOEC) and lowest observed effect concentration (LOEC) of post veliger were estimated at 30.0 psu and 25.0 psu, respectively, during 48-h exposure. The tolerance limits of the test species to salinity revealed various concentration ranges of salinity, which may reflect the physiology and ecology of the initial development stages of C. grata. These results demonstrate that reduced salinity is detrimental to the reproductive success and larval survival of C. grata, and if salinity is lowered by natural or anthropogenic sources during spawning, this would lead to decreased reproductive success and larval settlement.

• Journal of Plant Biology
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• v.36 no.3
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• pp.219-224
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• 1993

The effects of environmental factors on the growth of Enteromorpha compressa germlings from Daeyulri (34$^{\circ}$36'N; 127$^{\circ}$47'E), the southern cost of Korea were examined in laboratory culture through combinations of irradiance and salinity and temperature and salinity. They showed a maximum growth rate at 125 $\mu$E.m-2.s-1 and 32$\textperthousand$ of irradiance and salinity combination, and at 15$^{\circ}C$ and 32% of temperature and salinity combination. Optimal parameters for the growth of germlings were 15$^{\circ}C$, 125 $\mu$E.m-2s-1 and 32$\textperthousand$. The germlings of E. compressa were survived in a wide range of irradiance, temperature and salinity levels, even though they had relatively low irradiance optimum. In the field E. compressa occurred commonly during autumn and spring seasons and disappeared in summer, except for particular habitats. This may be caused by the salinity and water temperature of this area rarely drop below 26$\textperthousand$ and 8$^{\circ}C$ during winter. A broad tolerance to environmental and rapid growth of germlings made them a wide geographical distribution over the world and a survival in both the upper and lower intertidal zones.

• Korean Journal of Soil Science and Fertilizer
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• v.41 no.2
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• pp.83-93
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• 2008

Assessing for flora distribution is necessary for land management and environmental research in reclaimed lands. This study was conducted to find out the relationship between vegetation distribution and soil salinity on three reclaimed tidal flats of Kyonggi-bay in the mid-west coast of Korea. We investigated the soil salinity and identified the vegetation at the continuum distribution spots, and describe the characteristics of continuum distribution. On the reclaimed tidal flats, spatial variation of vegetation formed partially, however as the result for connection of each spatial variation along with the soil salinity, continuum distribution formed and it was overlapped edaphic gradient with vegetation distribution, it means that the continuum distribution correspond with soil salinity gradient, as the evidence high salt tolerance species occurred at high saline spots, non salt tolerance species occurred at low saline spots. On the aged reclaimed tidal flats, continuum type was various and also clearly distinguished but it was not clear on the early stage of reclamation. The continuum distribution distinguished sequential and non-sequential type. Sequential type started from high saline zone and connected to low saline zone gradually, on this type, vegetation changed from pioneer halophyte to facultative halophyte and glycophyte along with the salinity gradient. Non-sequential type formed by non-sequential change of soil salinity, on this type, vegetation distribution was non-regular form because it has not changed gradually. In the aged reclaimed land, vegetation wilted zone existed with high salinity, and continuum distribution started from this zone with bare patch.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.283-283
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• 2022

Abiotic stresses such as high/low temperature, drought, salinity, and submergence directly or indirectly influence the physiological status and molecular mechanisms of rice which badly affect yield. Especially, the low temperature causes harmful influences in the overall process of rice growth such as uneven germination and the establishment of seedlings, which has become one of the main limiting factors affecting rice production in the world. It is of great significance to find the candidate genes controlling low-temperature tolerance during seed germination and study their functions for breeding new rice cultivars with immense low-temperature tolerance during seed germination. In this study, 120 lines of Cheongcheong/Nagdong double haploid population were used for quantitative trait locus analysis of low-temperature germinability. The results showed significant difference in germination under low different temperature conditions. In total, 4 QTLs were detected on chromosome 3, 6, and 8. A total of 41 genes were identified from all the 4 QTLs, among them, 25 genes were selected by gene function annotation and further screened through quantitative real time polymerase chain reaction. Based on gene function annotation and level of expression under low-temperature, our study suggested OsGPq3 gene as a candidate gene controlling viviparous germination, ABA and GA signaling under low-temperature. This study will provide a theoretical basis for marker-assisted breeding.

• Proceedings of the Korean Society of Crop Science Conference
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• 2023.04a
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• pp.156-156
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• 2023

Salinity is a major abiotic stress that limits rice productivity in many regions of the world. In order to develop salt stress tolerant rice plants, genetic engineering is a promising approach. We characterized the molecular function of rice C3H2C3 as a really interesting new gene (RING). Oryza sativa RING finger protein H2-3 (OsRFPH2-3) was highly expressed in 100 mM NaCl. To identify the localization of OsRFPH2-3, we fused vectors that include C-terminal GFP protein (35S;;OsRFPH2-3-GFP). OsRFPH2-3 was expressed in the nucleus in rice protoplasts. An in vitro ubiquitin assay demonstrated that OsRFPH2-3 possessed E3-ubiquitin ligase activity. However, the mutated OsRFPH2-3 were not possessed any E3-ubiquitin ligase activity. Under salinity conditions, OsRFPH2-3-overexpressing plants exhibited higher chlorophyll, proline, SOD, POD, CAT, and soluble sugar contents and lower H₂O₂ accumulation than wild-type plants, supporting transgenic plants with enhanced salinity tolerance phenotypes. OsRFPH2-3-overexpressing plants exhibited low Na+ accumulation and Na+/K+ ratios in their roots. Theses results suggest that overexpression of OsRFPH2-3 can make plant insensitivity about salinity conditions.

• Journal of The Korean Society of Grassland and Forage Science
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• v.10 no.2
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• pp.77-83
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• 1990

A three year's field experiment was carried out on newly reclaimed tidal saline soils to evaluate the salt tolerance and growht characteristics, and their relationship to dry matter production and nutrient quality of main selected pasture species. Nine temperate grasses (14 varieties) and two forage crops (sorghum and pearl millet) were grown under different mulching treatments with medium sand and red-yellow soils (fine loamy materials of Typic Hapludults) from 1986 to 1988. Tall wheatgrass, tall fescue, reed canarygrass and alfalfa showed a good tolerance to soil salinity, especially tall wheatgrass (cv. Alkar) produced 19.6 ton/ha dry matter yield annualy under mulching treatment with medium sand depth in lcm. Pearl millet (cv. Gahi-3) was also evaluated as a salt tolerable forage species. Under salt stress in newly reclaimed tidal lands, plant showed a decrease in the assimirable leaf area (LA) as well as specific leaf area (SP. LA) and a low leaf weight ratio(LWR), and it resulted in a low concentration of crude protein and low digestible dry matter contents. Absorption of macro and micro elements in the plant on tidal lands was increased markedly.

• Animal Bioscience
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• v.37 no.6
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• pp.1130-1139
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• 2024

Objective: The daily maximum temperature and seawater level continuously increase as global warming continues. We examined the adaptability and production performance of heat-stressed goats with a supply of low-saline drinking water. Methods: Twelve Kacang and Kacang Etawah cross goats were exposed to two climatic conditions (control, 25℃ to 33℃, 83% relative humidity [RH], temperature humidity index [THI]: 76 to 86; and hot environment, 26℃ to 39℃, 81% RH, THI: 77 to 94) and two salt levels in drinking water (0% and 0.4% NaCl). The experimental design was a Latin Square (4×4) with four treatments and four periods (28 days each). Results: Temperature of the rectal, skin, and udder, and respiration rate rose, reached a maximum level on the first day of heat exposures, and then recovered. Plasma sodium rose at 0.4% NaCl level, while the hot environment and salinity treatments increased the drinking water to dry matter (DM) intake ratio. Water excretion was elevated in the hot environment but lowered by the increase in salinity. Total lying time increased, whereas change position frequency decreased in the hot condition. Lying and ruminating and total ruminating time increased and explained the enhanced DM digestibility in the hot conditions. Conclusion: The goats exhibited a high level of plasma sodium as salinity increased, and they demonstrated physiological and behavioral alterations while maintaining their production performances under increasing daily maximum temperatures.

• Journal of fish pathology
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• v.16 no.2
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• pp.119-123
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• 2003

We have selected an valuable pmbiotic strain; Lactobacillus sakei BK19 which has wide antagonic spectrum against fish pathogens . Present study investigated cultural characterization of L. sakei BK19 including pH tolerance , susceptibility of antibacterial agents and growth pattern with different environment such as nutritions, temperature and salinity. L. sakei BK19 showed Significantly higher resistance at low pH(around pH 4) environment and relative high antibiotic tolerance . In the study of optimal culture condition, maltose and saccharose provided the optimal nutritional culture condition while lactose and mannitol were unable to supply its carbon source for the fermentation of L. sakei BK19. Moreover. L. sakei BK19 showed good growth at the temperature range of 15 to $45^{o}C$ und the NaCl concentration of 0 to 7%. Hence, this particular probiotic strain may be benificial both in seawater and fresh weter conditions.

• Journal of Plant Biotechnology
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• v.4 no.3
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• pp.117-122
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• 2002

Cotton Glutathione S-Transferase(GST: EC 2.5.1.18) was cloned and Gh-5 cDNA was overexpressed in tobacco (Nicotiana tabacum) plants. The transformation of cotton GST in tobacco plant was confirmed by northern blot analysis. Type I and Type II transcript patterns were identified in Gh-5 transgenic tobacco plants. Type I transcripts was only discussed in this paper. Glutathione and 1-chloro-2,4-dinitrobenzene (CDNB) were used as the substrates, and the activity of GST in the type I transgenic plants was about 2.5-fold higher than the non-expressers and wild type tobacco plants. The expression of cotton GST in tobacco plants proved that Gh-5 could be translated into functional protein. Type I transgenic plants produced functional GST in the cells. Type I showed higher GST specific activity than Type II in the transgenic plants. Control and transgenic seedlings were grown in the growth chamber and under the light at 15$^{\circ}C$, and the effects of cotton GST in the seedlings was evaluated. The growth rate of Gh-5 overexpressors was better than the control and non-transgenic tobacco plants. Salinity tolerance was also analyzed on the seeds of transgenic plants. Seeds of Gh-5 overexpressors and the wild type tobacco seedlings were germinated and grown at 0, 50, 100, 150, and 200 mM NaCl solution. Gh-5 transgenic seedlings showed higher growth rate over control seedlings at both 50 and 100 mM NaCl solution. But at 0, 150, and 200 mM NaCl concentration, the difference in growth rate was not detected.

• Horticultural Science & Technology
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• v.34 no.6
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• pp.818-829
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• 2016

This study evaluated the responses of 18 potato cultivars to three levels of salinity stress (electrical conductivity, EC: 1.0, 4.0, and $8.0dS{\cdot}m^{-1}$). Stem, leaf, root, chlorophyll, tuber yield, and proline content were investigated and statistically analyzed using analysis of variance (ANOVA) and correlations. Stem number and stem diameter were not affected by salinity, but stem length and aerial weight showed highly significant responses to salinity. Aerial weight decreased with increasing salinity levels in most cultivars, while it increased in some the cultivars 'Daejima', 'Goun', 'Haryeong', and 'LT-8'. Leaf number, leaf area index, and leaf weight were most significantly affected by salinity and the cultivar ${\times}$ salinity interaction. Root length, root weight, total chlorophyll and chlorophyll a were affected by salinity, but not by the cultivar ${\times}$ salinity interaction. The opposite trend was shown in chlorophyll b. Although there was great variability among cultivars, tuber yield decreased in all cultivars, and was most significantly influenced by salinity and the cultivar ${\times}$ salinity interaction. 'Superior', 'Kroda', 'Romana', and 'Duback' gave better tuber yields under salinity at EC 4.0 and $8.0dS{\cdot}m^{-1}$ than the cultivars with better aerial weights. Proline content was increased by salinity in all cultivars, and was more remarkable in the cultivars with better aerial weights than in cultivars such as 'Superior' and 'Kroda' with better tuber yields. Leaf number, leaf area index, leaf weight, and root length parameters were considered to be useful criteria in the evaluation of salt tolerance because of their high positive correlation with tuber yield; however, given its negative correlation with tuber yield under high salinity, proline content was not. Salinity tolerances varied greatly among potato cultivars. The low correlation between growth and yields of aerial parts under high salinity suggests that, in commercial agriculture, it might be more practical to compare relative yields to controls. Additionally, 'Superior', 'Kroda', 'Romana', and 'Duback' might be very useful cultivars to use in breeding programs to develop salinity-tolerant potatoes, as well as for sustainable potato production in saline areas.