• Journal of Pharmaceutical Investigation
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• v.40 no.2
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• pp.101-107
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• 2010

A rapid and sensitive reversed-phase high performance liquid chromatography (HPLC) method was developed for the determination of N-(-4-Chlorophenyl)-6-hydroxy-7-methoxy-2-chromanecarboxamide (KAL-1120), a novel anti-inflammation agent, in the rat plasma. The method was applied to analyze the compound in the biological fluids such as bile, urine and tissue homogenates. After liquid-liquid extraction, the compound was analyzed on an HPLC system with ultraviolet detection at 275 nm. HPLC was carried out using reversed-phase isocratic elution with a $C_{18}$ column, a mobile phase of a mixture of acetonitril (40 v/v%) at a flow rate of 1.0 mL/min. The chromatograms showed good resolution and sensitivity and no interference of plasma. The calibration curve for the drug in plasma was linear over the concentration range of 0.05-50 ${\mu}g$/mL. The intra- and inter-day assay accuracies of this method ranged from 0.06% to 9.33% of normal values and the precision did not exceed 6.28% of relative standard deviation. The plasma concentration of KAL-1120 decreased to below the quantifiable limit at 1.5 hr after the i.v. bolus administration of 2-10 mg/kg to rats ($t_{1/2,({\alpha})}$ and $t_{1/2,({\beta})$ of 2.15 and 26.7 min at a dose of 2 mg/kg, 3.91 and 33.0 min at a dose of 10 mg/kg, respectively). The steady-state volume of distribution ($V_{dss}$) and the total body clearance ($CL_t$) were not significantly altered in rats given doses from 2 to 10 mg/kg. Of the various tissues tested, KAL-1120 was mainly distributed in the lung and heart after i.v. bolus administration. KAL-1120 was detected in the bile by 30 min after its i.v. bolus administration. However, the concentration in the urine after i.v. bolus administration became too low to measure, suggesting that KAL-1120 is mostly excreted in the bile. In conclusion, this analytical method was suitable for the preclinical pharmacokinetic studies of KAL-1120 in rats.

• Journal of the Korean Applied Science and Technology
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• v.34 no.4
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• pp.1076-1084
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• 2017

For analyzing the qualitative and quantitative analysis of fuel marker in petroleum products, the applicability of HPLC was studied. For the qualitative analysis of fuel marker in kerosene and automotive diesel, optimal analytical conditions(ratio of mobile phase solvent, flow rate, etc) in HPLC were selected and calibration curve for quantitative analysis of fuel marker was prepared based on the result of qualitative analysis. In particular, the correlation coefficient of calibration curve in kerosene and automotive diesel was shown to be 0.999 in a certain concentration range and it could be applied to the quantitative analysis. The results of analysis using the UV/Vis spectrometer, which is the current analysis method of fuel marker, were compared with the analysis results using the HPLC. The kerosene showed a low deviation of about 7 % and the automotive diesel showed a somewhat large deviation of about 20 %.

• Proceedings of the Korean Society for Emotion and Sensibility Conference
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• 1999.03a
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• pp.237-242
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• 1999

The nature of the interactions between the vestibular and autonomic systems is complex and has not been fully defined. Vestibuloocular reflex induced by sinusoidal rotation and activity of the autonomic nerves in the heart were measured to investigate the interactions between the vestibular system and the autonomic nervous system in healthy adults. Eye movement induced by sinusoidal rotation of the whole body or optokinetic stimulation at 0.04 Hz was analyzed in gain, phase, and symmetry. EKG was measured during vestibular stimulation and analyzed in heart rate variability including mean R-R interval, standard deviation (SD) and coefficient of variance (CV) of R-R interval, and power spectrum of low frequency region (LF) and high frequency region (HF). Gain of eye movement was 0.65${\pm}$0.03 by ratatory stimulation, 0.70${\pm}$0.02 in optokinetic stimulation, 0.08${\pm}$0.02 in visual suppression, and 0.84${\pm}$0.04 in visual enhancement. In R-R interval, resting condition (control) was 0.82${\pm}$0.03 sec, and visual suppression showed significant increase and visual enhancement did significant decrease compared with control (p<0.01).CV was 0.06${\pm}$0.02 in control and visual enhancement increased significantly (p<0.05). In LF/HF control was 1.40${\pm}$0.23, which was not different from rotatory or optokinetic stimulation. But visual suppression decreased LF/HF significantly and visual enhancement increased significantly compared with control (p<0.01). These results suggest that degree of gain corresponds with LF/HF and increased gain in visual enhancement is deeply related to the activity of sympathetic nerves.

• Journal of the Korean Society for Heat Treatment
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• v.36 no.6
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• pp.359-366
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• 2023

Changes in microstructure and mechanical properties of Mg-1.0Al-1.0Zn-0.2Mn-0.5Ca (AZMX1100) alloy sheet manufactured by normal casting and twin roll casting process, were studied according to process and heat treatment. Non-uniform microstructure was observed in the initial sheet produced through both processes, and in particular, tilted dendrites and shifted central segregation were observed in the twin roll casting sheet. It was homogenized through hot rolling and heat treatment, and heat treated at 350℃ and 400℃ to compare the effect of heat treatment temperature. Both sheets were homogenized by the hot rolling process, and the grain size increased as the heat treatment temperature and time increased. It was confirmed that the grain size, deviation, and distribution of the second phase were finer and more homogenized in the TRC sheet. Accordingly, mechanical properties such as hardness, formability, and tensile strength also showed better values. However, unlike other previously reported AZMX alloy systems, it showed low formability (Erichsen value), which was judged by the influence of Al2Ca present in the microstructure.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.3
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• pp.866-872
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• 2010

In this paper, we developed a digital soldering station based on PID controller, which supply stable power by controlling the current of heater of soldering iron. The proposed system designed PID controller to converge quickly to the set up temperature by user, and regain the lost of heat by external factors quickly. PID controller, designed by Ziegler-Nichols' tuning method, decides triac's trigger timing using setting temperature and present temperature to control the phase of AC 24V power that supply to the heater. Also, we give the function that shows present temperature and setting temperature of iron, and working time by graphic LCD. And during the rest time, we decided the power saving and extension of iron tip by dropping to the optimal temperature. Two experiments had implemented in $25^{\circ}C$ laboratory to confirm the performance of proposed method. The first experiment took 12sec, 13sec, 16sec, 18sec, reaching to $200^{\circ}C$, $300^{\circ}C$, $400^{\circ}C$, $480^{\circ}C$ respectively which result showed shorten of rising time than previous method. In the loading experiment of $300^{\circ}C$, $400^{\circ}C$, $480^{\circ}C$ steady state showed temperature drop of $3.8^{\circ}C$, $4.1^{\circ}C$, $4.5^{\circ}C$ which result showed the low temperature deviation than previous method.

• The Korean Journal of Nuclear Medicine
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• v.26 no.1
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• pp.40-48
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• 1992

Gated blood pool scan is frequently used for evaluating the change in cardiac function in various cardiac diseases. But resting gated blood pool scan using only LVEF as a cardiac index has been consitently shown to have a low sensitivity, which is about 50%, in detecting coronary artery disease. So it is recommended to compare exercise gated blood pool scan to resting gated blood pool scan. Exercise tests, however, are not always possible, especially in patients with musculoskeletal diseases, recent myocardial infarction and in elderly persons. We studied the usefulness of resting gated blood pool scan using multiple indices in evaluating the patients with coronary artery disease. Studied cases were 185 patients with coronary artery disease (angina pectoris 31, myocardial infarction 154) and 25 normals with low likelihood of coronary artery disease. We used $^{99m}Tc-labeled$ RBC, 740 MBq labeled by in vivo method. The data were evaluated by Micro DELTA computer program. The results were as following: 1) The ejection rates (PER, AER) and filling rates (PFR, AFR) were different in normls and patients with angina pectoris or myocardial infarction. 2) Mean phase angle, ejection rates and filling rates could separate normals from coronary artery disease patients with normal LVEF. 3) Regional ejection fraction was decreased at the site of the infarct in patients with myocardial infarction. 4) Peak filling rate was the the most detectable index in evaluation of cardiac function in patients with coronary artery disease. 5) The threshold at 1.5 standard deviation of normal range was considered as the most reliable cut-off value from ROC analysis. These data suggest that the resting gated blood pool scan has an important role in the evaluation of cardiac functional changes using various cardiac indices in patients with coronary artery disease.

• Journal of People, Plants, and Environment
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• v.24 no.4
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• pp.311-327
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• 2021

Background and objective: Recent urban agriculture meets the needs of urban residents that safety and avoids using chemical pesticides. This study was conducted to identify various factors of companion planting affecting the growth of cherry tomatoes, which will promoting urban agriculture by improving socioeconomic satisfaction with gardening activities through quality. Methods: Four types of companion plants such as marigold, zinnia, spearmint and basil, that have a companion effect with growth, sugar content, and vitamin C content. We obtained the mean and standard deviation and tested the significance at a 95% confidence level (p < .05) with Duncan's multiple range test after one way ANOVA and MANOVA. Results: Compared to monoculture of cherry tomatoes, the plant growth in the treatment plots with companion planting showed a significant increase overall(p < .05), but there wasn't interaction effect among companion plants, planting ratio and type. As for the absorption of inorganic components, the companion planting showed better absorption than monoculture of cherry tomatoes, as favorable growth, and there was an interaction effect among the individual factors. The sugar content was higher than the standard sugar content of 5.8 brix in both the treatment plots at the control, and vitamin C content was higher than the control at 26.27mg/100g in all treatment plots, but there wasn't statistically significant difference. The soil pH in the cultivation plot ranges from 5.5 to 9.0 and was weakly alkaline in all treatment plots except zinnia, showing low contents of phosphoric acid, exchageable potassium, calcium and magnesium. Conclusion: This study was conducted to analyze various factors such as the growth of cherry tomatoes, contents of inorganic components, sugar content and vitamin C content of fruits, and soil analysis according to companion plants, planting type, and planting ratio. We will study sugar content by measuring the change in growth every phase of fruits.

• The Journal of the Society of Stroke on Korean Medicine
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• v.11 no.1
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• pp.1-8
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• 2010

Objectives : We designed this study to investigate difference of heart rate variability(HRV) according to sex, age, acute or chronic phase, category of stroke, national institute of health stroke scale(NIHSS), lesion of stroke. Methods : 64 subjects were recruited from the patients admitted to the department of oriental medicine at East-West Neo Medical Center, Kyung Hee University from 1 September 2009 to 31 August 2010. We compared heart rate(HR), standard deviation of all normal P-P intervals(SDNN), low frequency(LF), high frequency(HF), LF/HF ratio. Results and Conclusions : 1. LF/HF ratio is significantly different between over-70 and below-70 of age. 2. SDNN is significantly different between acute and chronic stroke patients. 3. In sex, category of stroke, national institute of health stroke scale(NIHSS), lesion of stroke, there are no significantly different among the any values of heart rate variability(HRV).

• Journal of the Society of Cosmetic Scientists of Korea
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• v.49 no.4
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• pp.291-298
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• 2023

In this study, a quantitative analysis method was developed using high-performance liquid chromatography (HPLC) to analyze the content of ceramide NP in lotion, cream, and cleanser formulations in cosmetics. The analysis was performed using a C18 column, and the mobile phase was set at a ratio of 70 : 30 for acetonitrile and methanol, the flow rate was set to 0.8 mL/min, and the column temperature was set to 20 ℃. The method was verified by analyzing specificity, linearity, limit of detection, limit of quantitation, accuracy, and precision in accordance with the ICH guidelines. As a result of validating the method, the linearity of the calibration curve was excellent (R² = 0.99984). The accuracy of the lotion, cream, and cleanser formulations was confirmed with a recovery rate ranging from 95.11% to 100.48%. The precision analysis showed a low relative standard deviation (RSD) of less than 0.26%. The limit of detection was 0.902 ㎍/mL, and the limit of quantitation was 2.733 ㎍/mL. Through this quantitative analysis method of ceramide NP applied in cosmetics, it is expected to assist in the quality control of products by enabling measurement even when it is difficult to separate the main peak due to the influence of interfering substances.

• Korean Journal of Applied Biomechanics
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• v.15 no.1
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• pp.237-257
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• 2005

It was to study as a following-research of "A Case Study on Center of Gravity(COG) Analysis when Performing Uchimata(inner thigh reaping throw) by Posture and Voluntary Resistance Levels(VRL) of Uke in Judo[I]". The purpose of this study was to analyze the COG variables when performing uchimata(inner thigh reaping throw) by two postures and voluntary resistance levels(VRL) of uke(reciver) in Judo. The subjects, who were one male judoka(YH) for 1992 Barcelona Olympic Games Olympian(silver medalist), and one male trainee; Y.I.University representative member (SDK), and were filmed on two S-VHS 16mm video cameras(60fields/sec.) through 3-dimensional motion analysis methods, that postures of uke were shizenhontai (straight natural posture) and jigohontai(straight defensive posture), VRL of uke were 0% and 100%, respectively. The kinematical variable was COG variable, distance of COG, and distance of resultant COG between uke and tori(the thrower), velocity and acceleration of COG. The data of this study collection were digitized by SIMI Motion Program computed the mean values and the standard deviation calculated for each variables. When performing uchinmata according to each posture and VRL of uke and classifying. From the data analysis and discussion, the conclusions were as follows : 1. Displacement of COG Subject YH, COG was the highest in kuzushi(balance -breaking), vertical COG was low when following in tsukuri(positioning; set-up), kake(application; execution), and COG was pattern of same character each postures and resistance, respectively. Subject SDK, COG was low from kumikata(engagement positioning) to kake, and COG was that each postures and resistance were same patterns, respectively. Subject YH, SDK, each individual, postures and resistance, vertical COG was the lowest in kake phase, when performing. 2. Distance of COG between uke and tori The distance of COG between uke and tori when performing, subject YH was $0.64{\sim}0.70cm$ in kumikata, $0.19{\sim}0.28cm$ in kake, and SDK was $0.68{\sim}0.72cm$ in kumikata, $0.30{\sim}0.42\;cm$ in kake. SDK was wider than YH. 3. Distance of resultant COG between uke and tori The distance of resultant COG between uke and tori when performing, subject YH was $0.27{\sim}0.73cm$ from kumikata to kake. and SDK was $0.14{\sim}0.34cm$ in kumikata, $0.28{\sim}0.65cm$ in kake. Jigohontai(YH:$0.43{\sim}0.73cm$,SDK:$0.59{\sim}0.65cm$) was more moved than shizenhontai(YH:$0.27{\sim}0.53cm$, SDK: $0.28{\sim}\;0.34cm$). 4. Velocity of COG The velocity of COG when performing uchimata, subject YH was fast anterior-posterior direction in kuzushi, ant.-post. and vertical direction fast in tsukuri and kake. SDK was lateral, ant.-post. and vertical direction in kuzushi, ant.-post. and vertical direction in tsukuri and ant.-post. direction in take, respectively. 5. Acceleration of COG The acceleration of COG when performing uchimata, The trend of subject YH was showed fast vertical direction in kuzushi and tsukuri, ant.-post. and vertical direction fast in kake. The trends of SDK showed lateral direction in kuzushi, lateral and ant.-post. direction in tsukuri and ant.-post. direction in kake, respectively.